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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 6' ETHYL SPIRO 1 3 DIOXANE 2 3' INDOLIN 2' ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
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bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
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bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...
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bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... Caspase-3/7 activation in live cells was monitored using CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific, C10423, 1:1000) in IncuCyte Live Cell Analysis System ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were washed in 3 times in 3% PBST (10 min) and incubated in goat anti-chicken Alexa Fluor 488 (Invitrogen, A11039, 1:1000) in blocking buffer at room temperature for 2hrs ...
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bioRxiv - Developmental Biology 2024Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16°C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3).
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bioRxiv - Molecular Biology 2023Quote: ... Naïve hESCs were routinely passaged as single cells every 3 days at a ratio of 1:3 using TrypLE™ Express (1X) (Gibco, Thermo Fisher Scientific) and plated in fresh media supplemented with 10 μM of Y-27632 (Stemcell Technologies).
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (5,000 cell/well) ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2020Quote: ... The qRT-PCR assays were performed to quantify the amount of SARS-CoV-2 RNA with QuantStudio 3 instrument (Thermo Fisher Scientific, Waltham, MA, USA) using primers and probes described previously8,9 (N2 set or E set).
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™, Thermo Fisher Scientific, cat. no. V13154). Cells were plated in triplicate in a 96-well plate (3,000 cell/well in 200 μL of complete medium and cultured under standard conditions for 48 h ...
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bioRxiv - Neuroscience 2021Quote: ... Hippocampal neurons were seeded onto polyethylenimine-coated 3-cm dishes (2 × 105 cells/dish) and cultured in neurobasal medium (Gibco; Thermo Fisher, Waltham, MA, USA) supplemented with B-27 and 2 mM Glutamax (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Each sample was injected onto a PepMap C18 trap column (75 μM X 2 cm, 3 μM, 100 Å, Thermo Fisher Scientific, San Jose, CA) at 5 μL/min for 6 min using 0.05% trifluoroacetic acid (TFA ...
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bioRxiv - Evolutionary Biology 2023Quote: ... with each sample being prepared from 5 individuals (3 karyotypes x 2 temperatures x 9 isochromosomal lines = 54 samples) using the MagMAX™-96 Total RNA Isolation Kit (ThermoFisher Scientific, Waltham, MA, USA) on a MagMAX™ Express Magnetic Particle Processor (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA). Viral supernatants were collected 48 hours post-transfection and filtered through a 0.45 μm Nalgene syringe filter SFCA (Whatman ...
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bioRxiv - Biochemistry 2020Quote: ... HEK 293 cells were plated onto 6-well plates (2.5-4.5 × 105 cells per well) in 3 ml of MEM (Life Technologies) with 10% FBS (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were incubated with lentiviral supernatant and 3 days later selection begun with 6 μg/mL puromycin (Thermo Fisher), which was maintained during all subsequent culturing.
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Microbiology 2022Quote: ... and N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine (biotin-X DHPE; Molecular Probes, Life Technologies). Planar bilayers were formed from 200-nm liposomes in channels of a PDMS flow-cell using vesicle spreading method (36) ...
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bioRxiv - Molecular Biology 2020Quote: ... flushed with argon prior to adding hydrochloric acid (3 mL of 6 M sequencing grade solution; Thermo Scientific #PI24308). Sealed tubes were kept at 125° C for 48h (oil bath ...
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bioRxiv - Physiology 2019Quote: ... Each 6 μL assay master mix (AMM) consisted of 3 μL of TaqMan® Gene Expression assay 20X (ThermoFisher) and 3 μL of 2X Assay Loading Reagent (Fluidigm) ...
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bioRxiv - Evolutionary Biology 2020Quote: 3-6-day old female ovaries were dissected from each experimental genotype and placed directly in Trizol reagent (Invitrogen), and homogenized ...
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bioRxiv - Neuroscience 2024Quote: ... n=6) before being perfused intravenously with fluorescent Texas Red 40 kDa Dextran (3 mg/kg; D1829, Fisher Scientific) as described in previous studies (25,26 ...
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bioRxiv - Microbiology 2023Quote: ... coverslips were washed once with 0.1M MOPS buffer (pH 3) and stained with 50 μg / ml 5,(6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen) in MOPS buffer for 1 h at 37°C in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... The parental hiPS cell line (8858-3) was maintained in 6-well plates using StemFlex medium (Life Technologies, A3349401). Cas9 ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Neuroscience 2021Quote: ... fixed larvae were stained with 2.5 ug/ml 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS to label all cell nuclei ...
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bioRxiv - Molecular Biology 2019Quote: ... 6’-Diamidino-2-phenylindole (DAPI; Cat#D1306; RRID: AB_2629482) were purchased from Invitrogen (Carlsbad, CA). Neurobasal® medium ...
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bioRxiv - Immunology 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and LysoTracker Red DND-99 were purchased from Invitrogen, Carlsbad ...
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bioRxiv - Microbiology 2019Quote: ... nuclei were stained using Prolong gold antifade containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). All preparations were observed with a confocal microscope (ZEISS LSM 700 inverted ...
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bioRxiv - Genomics 2021Quote: ... the cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, R37606). Cells were then viewed and photographed using a fluorescence microscope (Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... Mounting media with DAPI (4’,6-diamidino-2-phenylindole) used was either ProLong Gold (Invitrogen) or ProLong Diamond (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... cell nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, dilactate; 2μM, Life Technologies). Cells were visualized in PBS with calcium and magnesium (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were stained with 4′-6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, CA, USA) before examination under a fluorescence microscope (Zeiss LSM 710).
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bioRxiv - Microbiology 2020Quote: ... cell nuclei were stained with DAPI (4 =,6 =-diamidino-2-phenylindole) (Thermo Fisher Scientific, D1306) and mounted on slides with Prolong diamond antifade mounting medium (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... 2 µg DNA and 6 µL PEI were incubated with 50 µL Opti-MEM (Gibco) seperately for 5 minutes before combining and incubating for 20 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The culture medium was replaced with fura-2 AM (6 μM, F1221; Thermo Fisher Scientific) in Opti-MEM (11058-021 ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2ng/ml; Molecular Probes). Acti-stain 555 phalloidin (Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2022Quote: ... Cell nuclei of HUVECs were stained by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, D1306) at 1:500 dilution for 15 min ...
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Involvement of nucleus accumbens D2-MSN projections to the ventral pallidum in anxious-like behaviorbioRxiv - Neuroscience 2022Quote: ... All sections were stained with 4’,6-diamidino-2-phenylindole (1mg/ml; Invitrogen, MA, USA) and mounted using Permafluor (Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... 6-Diamidino-2-Phenylindole (DAPI, Ex 360 nm and Em 460 nm, Thermo Fisher, D1306) or (SYBR Green I ...
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bioRxiv - Biophysics 2020Quote: ... Di-4-ANEPPDHQ and Laurdan (6-dodecanyl-2-dimethylaminonaphtalene) were from Molecular Probes (Eugene, OR). L-α-phosphatidylcholine from chicken egg (EggPC) ...