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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... either with 1 μM FluoZin-3-AM (Thermo Fisher scientific) at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... 3-Isobutyl-1-methylxanthine (IBMX) was from Acros Organics (#228420050). PF-04447943 was purchased from MedChem Express (#HY-15441/CS-0942) ...
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bioRxiv - Neuroscience 2023Quote: ... Anti-Adenylate Cyclase 3 (IF: 1:500, Invitrogen, PA5-35382); Anti-Ephrin-B1 (IF ...
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bioRxiv - Microbiology 2022Quote: ... with a 1:3 ratio of siRNA:RNAiMax (Thermo Fisher Scientific). 48 hours post-transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... All lines were maintained in 3:1 DMEM (Gibco, 11960044): F12 media (Gibco ...
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bioRxiv - Bioengineering 2022Quote: 1” x 3” glass microscope slides (12-550-A3, ThermoFisher) were cleaned with 0.1% Triton X-100 (T9284 ...
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bioRxiv - Synthetic Biology 2022Quote: ... at 3:1 bead:cell ratio in AIM-V media (Gibco) supplemented with 5% FBS ...
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bioRxiv - Neuroscience 2023Quote: ... in HBSS and 1 μM YOYO-3 iodide (Thermofisher, Y3606) in the culture media ...
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bioRxiv - Immunology 2024Quote: ... at a mass ratio of 1:3 in OptiMEM (ThermoFisher). After incubating for 25 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were diluted (1:3) using loading buffer (ThermoFisher), heat-denatured for 3 minutes at 95°C ...
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bioRxiv - Biophysics 2021Quote: ... A Methyl-β-Cyclodextrin solution (200 mM) in Live Cell Imaging Solution (Thermo Fisher Scientific) was added to the cholesterol lipid film at a molar ratio of 10 to 1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin was stained with To-Pro-3 (1/200 dilution, 1 mM, Invitrogen T3605).
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bioRxiv - Neuroscience 2022Quote: ... dried and coverslipped under glycerol:TBS (3:1) with Hoechst 33342 (1:1000; ThermoFisher Scientific). Primary antibodies with the following dilutions used in this study include ...
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bioRxiv - Biochemistry 2024Quote: ... columns were washed with 3 column volumes (CV) of 1:1 RNase ZAP (Ambion) followed by 3 CV nuclease-free water (DEPC-treated MilliQ water) ...
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bioRxiv - Pathology 2024Quote: ... and 3-hydroxybutyrate dehydrogenase 1 (15417-1-AP, Thermo Fisher Sci, Waltham, MA, USA). HRP-conjugated secondary antibodies (NA9340 ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody (2-3% serum, 0.4% PBST, 1:500 Invitrogen A11035, 1:500 DAPI) was applied and incubated for one hour at room temperature ...
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bioRxiv - Bioengineering 2021Quote: ... and non-essential amino acids (10 mM, glycine, L-alanine, L-asparagine, L-aspartic acid, L-glutamic acid, L-proline, L-serine; Gibco). The pre-incubated viruses were then applied to Vero-E6 cell monolayers ...
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bioRxiv - Systems Biology 2021Quote: Cells were grown in Iscove’s Modified Dulbecco’s Medium (IMDM) medium +10% FBS +1% Non Essential Amino Acids +1% PennStrep (Gibco). All transfections used the Invitrogen Neon electroporation machine using a 100 ul tip ...
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bioRxiv - Neuroscience 2021Quote: ... Values of Rmin were obtained in situ by incubating preparations in Ca2+-free HL6 with 1 mM EGTA and 100 mM BAPTA-AM (1% DMSO, 0.2% Pluronic Acid) for 20min (Cat.No. B6769; Invitrogen). Values of Rmax were obtained in situ by incubating preparations in HL6 containing 10 mM Ca2+ and 100 μM ionomycin (Cat.No ...
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bioRxiv - Microbiology 2024Quote: ... 2% B27, 1% N2, 1% MEM non-essential amino acids, 1% penicillin/streptomycin and 0.1% β-mercaptoethanol, all from ThermoFisher Scientific) supplemented with dual SMAD inhibitors (10 µM SB-431542 and 100 nM LDN-193189) ...
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bioRxiv - Biophysics 2022Quote: ... and 2-3 μL of the freshly phase-separated sample was placed into a chamber made on a glass slide (Fisher Scientific 3” × 1” × 1 mm). The chamber made by using double-sided tape was then sealed with a square coverslip to avoid evaporation of the sample ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with following primary antibodies and/or labelling agents: anti-GFP (Invitrogen, #11122, 1:800, 3 days or Invitrogen, #10262, 1:800, 3 days), anti-V5 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Nucleic acids were stained using SYBR gold Nucleic Acid Gel Stain (Invitrogen) and visualized using Gel Doc™ XR+ (Bio-Rad) ...
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bioRxiv - Biochemistry 2022Quote: ... formic acid (FA) and trifluoroacetic acid (TFA) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Physiology 2020Quote: Caspase-3-like activity was quantified using the EnzChek Caspase-3 Assay kit #1 (Molecular Probes, Eugene, OR, USA). Tissue samples were thawed on ice for 5 min ...
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bioRxiv - Biophysics 2020Quote: ... hexanoyl)-2-(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-pentanoyl)-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine) (Invitrogen). Cleavage of PED-6 eliminates a self-quenching effect that results in the release of a BODIPY-FL dye ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: Fibroblasts were plated in 96-well plates (10,000 fibroblasts/well) for 3 days in 2% FBS in DMEM supplemented with ascorbic acid (50 μg/mL) (Fisher Scientific, Waltham, MA, USA). Fibroblasts and matrix were then fixed and immunostained as detailed above ...
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bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
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Controlled Fluorescent Labelling of Metal Oxide Nanoparticles for Artefact-free Live Cell MicroscopybioRxiv - Biophysics 2021Quote: ... 3.2 μL of 12.1 mM fresh Alexa Fluor 488 or 647 – SDP ester (Life Technologies, Alexa Fluor 488 or Alexa Fluor 647 ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2 mg SSHELs were labeled with 10 µM of Alexa 488 Succinimidyl Ester (Life Technologies) for 1 h at room temperature prior to conjugation with affibodies ...
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bioRxiv - Genetics 2020Quote: ... that had been conjugated with Alexa647 using the N-hydroxysuccinimidyl ester (Molecular Probes, Eugene, OR) of this fluorophore and 2 µg/mL Alexa488-conjugated goat anti-chicken IgG (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... Transgenic lymph node cells were labelled with 5-carboxyflurorescein diacetate succinimidyl ester (CFSE) (Molecular Probes) as described (14 ...
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bioRxiv - Biophysics 2020Quote: ... SgrS and RyhB probes were labeled with Alexa Fluor 647 NHS ester (A647, Invitrogen A20006). The16S rRNA probe was labeled with Alexa Fluor 405 NHS ester (A405 ...
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Dissociation of β2m from MHC Class I Triggers Formation of Noncovalent, Transient Heavy Chain DimersbioRxiv - Cell Biology 2022Quote: Purified antibodies were labeled with Alexa Fluor 647 NHS ester (Thermo Fisher Scientific, Darmstadt, Germany) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2022Quote: ... SKM was prelabeled with Alexa Fluor™ 568 NHS Ester (Thermo Fisher Scientific, Waltham, MA). SKM was incubated with the dye on ice for an hour to ensure complete binding ...
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bioRxiv - Biophysics 2022Quote: ... and were conjugated with biotin and silicon rhodamine (SiR) NHS esters (Invitrogen: B1582, Spirochrome: SC003) to produce “SiR-biotin-Fab” ...
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bioRxiv - Immunology 2020Quote: ... Purified Pf merozoites were stained with pHrodo ™ iFL Red STP amine-reactive ester (ThermoFisher) at 8 M in RPMI and incubated to allow for opsonization with 2 mg/ml plasma IgM or IgG antibodies for 1 h at RT ...
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bioRxiv - Microbiology 2021Quote: ... target cells were labelled with 100 ng/ml of pHrodo Green STP ester (Invitrogen, P35369) pH 7.8 for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... The samples were treated with 0.1 mg/ml Acryoloyl-X succinimidyl ester (Thermo Fisher, A20770) in 1x PBS for 30 minutes at room temperature ...
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bioRxiv - Pathology 2021Quote: ... thymocytes were labeled with carboxyfluorescein succinimidyl ester (CFSE; CellTrace CFSE dye, Invitrogen, Eugene, OR, USA) according to manufacturer’s instructions and exposed to UV radiation (254 nm ...
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bioRxiv - Immunology 2021Quote: Autologous B-cells were stained with 0.5μmol/L carboxyfluoroscein succinimidyl ester (CFSE, Thermo Fisher Scientific) before wild-type or variant peptide loading at 1μg/mL for one hour ...
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bioRxiv - Microbiology 2021Quote: ... and 4 mg/l 5-(and-6)-carboxyfluorescein and succinimidyl ester (FITC; Thermo Fisher Scientific), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... Purified integrins were labelled with Alexa Fluor 647 succinimidyl ester following the manufacturer’s protocol (Thermofisher). Integrins were frozen in liquid nitrogen and stored at −80◦C in a buffer containing detergent (20 mM Hepes pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... SuperSignal Chemiluminescent substrate and 2’,7’-dichlorodihydrofluorescein diacetate acetyl ester (H2DCFDA) were from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The purified monocytes were labelled with carboxyfluorescein diacetate succinimidyl ester (CFSE, 10 μM, V12883, ThermoFisher) and 2.5×106 cells injected intraveneously via the tail vein ...
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bioRxiv - Physiology 2023Quote: Isolated cardiomyocytes were briefly incubated with 0.2 µM Fura-2-acetoxymethyl ester (Fura-2AM, Invitrogen), a calcium sensitive ...
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bioRxiv - Plant Biology 2023Quote: ... 0.1% Triton X–100 and 400 μM Maleimidobenzoyl–N–hydroxy succinimide ester (ThermoFisher Scientific, 22311), washed two times with 1x Phosphate–buffered saline (PBS ...
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bioRxiv - Cell Biology 2024Quote: ... they were exposed to 100 μL of 2.5 μM TMRE (tetramethylrhodamine, ethyl ester; Life Technologies) that covered the bacterial lawn ...