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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 3 Cyclohex 1 enyl acrylic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 1% Minimum Essential Medium (MEM) Non-Essential Amino Acids (Thermo Fisher Scientific, 11140050), and 1% Penicillin-Streptomycin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1% minimum essential medium non-essential amino acids (Gibco/Fisher Scientific, Hampton, NH), 25 mM HEPES (Gibco/Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 1% minimum essential medium non-essential amino acids (Gibco/Fisher Scientific, Hampton, NH), 25 mM HEPES (Gibco/Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... reaction was quenched by adding 1% trifluoroacetic acid (TFA, Fisher Scientific, O4902-100). The samples were desalted using Peptide Desalting Spin Columns (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 1% MEM Non Essential Amino Acids Solution 100x (Fisher Scientific 11-140-050), 1% sodium pyruvate 100mM (Life Technologies 11360070),1% penicillin-streptomycin-glutamine 100x (Life Technologies 10378016) ...
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bioRxiv - Neuroscience 2024Quote: ... reaction was quenched by adding 1% trifluoroacetic acid (TFA, Fisher Scientific, O4902-100). The samples were desalted using Peptide Desalting Spin Columns (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... containing 1mM Probenecid and 20mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco). Cells were imaged at Ex/Em = 490/525nm for Cal-520 AM fluorescence intensity for a total of 120 seconds ...
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bioRxiv - Biophysics 2024Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... or amino acids (MEM amino acids solution; Gibco, Waltham, MA, USA).
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bioRxiv - Biochemistry 2024Quote: ... Except for lauric acid (Henkel) and 1,18-Octadecanedicarboxylic acid (Thermofisher Scientific), lipids were from Merck ...
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ISL2 is an epigenetically silenced tumor suppressor and regulator of metabolism in pancreatic cancerbioRxiv - Molecular Biology 2020Quote: ... cells were stained by BODIPY™ FL C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific #D3922) to determine the amount of lipid droplet in regular conditions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The dried peptide samples were re-dissolved in 0.1% formic acid and loaded onto a C18 column (100 μm× 20 mm, 3 μm, Thermo Fisher Scientific, USA) then separated on a C18 analytical column (150 μm × 120 mm ...
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bioRxiv - Microbiology 2021Quote: Challenged A549 cells were lysed with modified RIPA buffer (Supplementary Data 3) and protein concentration was determined using a bicinchoninic acid (BCA) protein assay (Thermo Fisher Scientific). Phospho-p38 (p-p38) ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were reconstituted in LC-MS injection buffer (3% acetonitrile, 0.1% formic acid) and quantified by Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Product #23290).
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bioRxiv - Genetics 2020Quote: ... 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene 3-dodecanoic acid (BODIPY™ FL C12; Thermo Fisher Scientific, USA) (4 µg/mL ...
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bioRxiv - Microbiology 2022Quote: ... on the cell membrane potential was assessed by using the bis-(1,3-dibutylbarbituric acid) trimethine oxonol - DiBAC4(3) reagent (Invitrogen, Carlsbad, CA, USA) according to Veerana et al. ...
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bioRxiv - Microbiology 2022Quote: ... Roughly 1/3 of the plate (2-3 loopfuls) were added to 400 µl 1x Tris-Ethylenediaminetetraacetic acid (EDTA) pH 7.5 (Thermo Scientific, Waltham, USA) and heated at 80 ºC for 30 min ...
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bioRxiv - Biochemistry 2023Quote: Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Molecular Biology 2023Quote: ... P8 pups were fed 1 μl 4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Hexadecanoic Acid BODIPY™ FL C16 (BODIPY FL C16, ThermoFisher Scientific, D3821) diluted in olive oil at a concentration of 10 μg/μL ...
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bioRxiv - Pathology 2023Quote: Dried peptide samples were dissolved in 4.8 μL of 0.25% formic acid with 3% (vol/vol) acetonitrile and 4 μL of each sample was injected into an EasynLC 1000 (Thermo Fisher Scientific). Peptides were separated on a 45 cm in-house packed column (360 μm OD×75 μm ID ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting tryptic peptides were extracted by adding 50% digest dilution buffer (60 mM AB solution with 3% formic acid) and injected into a HPLC system coupled to a hybrid LTQ-Orbitrap XL mass spectrometer (Thermo Fisher Scientific) via a nano-electrospray ion source ...
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bioRxiv - Cancer Biology 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Biochemistry 2024Quote: ... Sample digests were acidified with formic acid to a pH of 2-3 before desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for liquid chromatography-mass spectrometry analysis.
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bioRxiv - Immunology 2022Quote: ... were harvested bilaterally into cold complete RPMI media (cRPMI; 10% FBS [Gibco], 1% penicillin/streptomycin [Gibco], 1% sodium pyruvate [Gibco], 1% non-essential amino acids [Gibco] ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (EuroBio) and 1% Pen/Strep + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids solution (Gibco) at 37°C in 5% CO2 humidified incubators ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 700 μL complete RPMI media (cRPMI; 10% FBS [Gibco], 1% penicillin/streptomycin [Gibco], 1% sodium pyruvate [Gibco], 1% non-essential amino acids [Gibco] ...
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bioRxiv - Developmental Biology 2024Quote: ... Single-cell suspensions were cultured in the culture medium (MEMα + 20% FBS + 1% penicillin-streptomycin + 1 [v/v%] 100× non-essential amino acid solution [NEAA; Gibco], 1 [v/v%] 100× GlutaMAX [Gibco]). At 80%– 90% confluence ...
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bioRxiv - Cell Biology 2024Quote: ... a premium microscope slide (Fisher-finest, 3″ × 1″ × 1 mm; Thermo Fisher Scientific, 12-544-1) or chambered coverglass (1 well ...
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bioRxiv - Cell Biology 2020Quote: ... 5 micromols sodium sulfo-NHS and 5 micromols 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Thermo Fisher #22980) in 10 μL of DMSO ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Immunology 2020Quote: ... and 50 µL of 50 mg/mL 1-ethyl-3-[3-dimethyl-aminopropyl]-carbodiimidehydrochloride (Thermo Fisher Scientific, 22981) were simultaneously added to the reaction tubes ...
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bioRxiv - Biochemistry 2020Quote: ... 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) and N-hydroxysulfosuccinimide (Sulfo-NHS) were obtained from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Immunology 2023Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Bioengineering 2024Quote: ... EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride) and Sulfo-NHS (N-hydroxysulfosuccinimide) were purchased from ThermoFisher (USA). Unless otherwise stated all other chemicals were received from Sigma (St Louis ...
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bioRxiv - Cancer Biology 2020Quote: ... we added 1:20-1:100 (volume) of 1 mg/ml fluorescent tag (Alexa Fluor® succinimidyl esters of the desired excitation/emission spectrum, Invitrogen™, Molecular Probes®) to the gel premix immediately before polymerization.
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bioRxiv - Biophysics 2021Quote: ... 1 µM of TO-PRO-3 iodide (Thermo Fisher, T3605) was added for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... anti-CENP-A (Clone 3-19, Invitrogen; 1:1000 dilution) and anti-“Bonsai”/NDC80 (68 ...
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bioRxiv - Cell Biology 2021Quote: ... anti-Lamin B2 (mouse, Invitrogen, clone E-3, 1:500), anti-EEA1 (mouse ...
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bioRxiv - Cell Biology 2020Quote: ... 50 μg mL−1 Cascade Blue (3 kDa, Thermo Fisher) was added to the antibiotic-free expansion media in the top epithelial channel with a flow rate of 30 μL hr−1 ...
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bioRxiv - Immunology 2022Quote: ... cDNA was diluted 1:3 in nuclease-free water (Ambion) and added to 5 μL SyGreen HiROX mix (PCR Biosystems ...
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bioRxiv - Neuroscience 2022Quote: ... rat anti-Galectin-3 (1:200, Invitrogen, 14-5301-82); rabbit anti-Fibronectin (1:500 ...
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bioRxiv - Bioengineering 2022Quote: ... or Toto-3 iodide (T3604, Invitrogen, MA, USA; 1:500) diluted in PBST ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were counterstained with TO-PRO-3 (1:5,000, Invitrogen) together with secondary antibodies when necessary ...
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bioRxiv - Microbiology 2020Quote: 1 μL of random primers at 3 μg/μL (Invitrogen) were added to fragmented RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... nuclei were stained with 1 µg/mL TOTO-3 (Invitrogen) in PBS for 5 minutes ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Transfection was performed using 1:3 DNA: Lipofectamine 2000 (Invitrogen) ratio ...
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bioRxiv - Cancer Biology 2021Quote: ... mouse anti-INSR (1:50, clone CT-3, Invitrogen, AHR0271).