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Citations for Thermo Fisher :
1901 - 1950 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: Osteoblasts on glass coverslips were loaded with 2 µM Fura-2 AM-ester (Invitrogen) for 30 minutes at 25 °C in 107 mM NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed three times with 2× saline sodium citrate (2×SSC, ThermoFisher AM9765), and equilibrated with encoding-probe wash buffer (30% formamide (ThermoFisher AM9342 ...
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bioRxiv - Immunology 2020Quote: ... Fluorescent dyes including 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, D21490, 2 μg/mL), wheat germ agglutinin (WGA ...
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bioRxiv - Immunology 2020Quote: ... 2 mM L-Glutamine and 50 µm 2-Mercaptoethanol) with 10% FCS (all Gibco). Colon and caecal tissue were digested in an enzyme cocktail of 0.45 mg/ml collagenase V (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Caco-2 cells were seeded into LabTekII 2-well chambered coverglass (#155380, ThermoFisher Scientific) pre-coated with 0.01% Poly-L-lysine (#P4707 ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: ... Fluorescent dyes including 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen, D21490, 2 μg/mL), wheat germ agglutinin (WGA ...
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bioRxiv - Microbiology 2021Quote: ... 2% fetal bovine serum (S182H-500, Eurobio) and N-2 Supplement (17502048, Thermo Fisher) at 37°C and 5% CO2.
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bioRxiv - Neuroscience 2023Quote: ... and 5ng/mL bovine fibroblast growth factor-2 (bFGF-2, #13256-029, Gibco™)) (proliferative conditions) ...
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bioRxiv - Cancer Biology 2023Quote: ... animals were subjected to intracardiac perfusion with 2% PFA-2% glutaraldehyde (A17876, ThermoFisher Scientific) in 0.1 M phosphate buffer (PB ...
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bioRxiv - Genomics 2023Quote: ... and 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Gibco 15630080), and incubated at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... (2) cells were incubated with 2 μg/mL BODIPY FL Vancomycin (Thermo Scientific, V34850) for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 2% SDS) and 2 µL 20 mg/mL proteinase K (25530049, Thermo Fisher Scientific). Samples were incubated overnight at 65 °C and 800 rpm ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 µl 10% SDS plus 2 µl proteinase K (Thermo Fisher Scientific, Cat# EO0492) were added and incubated for 60 min 37°C ...
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bioRxiv - Cell Biology 2024Quote: Fura-2 acetoxymethyl ester (fura-2/AM) was from Molecular Probes (Leiden, The Netherlands). High-glucose Dulbecco’s modified Eagle’s medium (DMEM) ...
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bioRxiv - Neuroscience 2024Quote: ... and then triturated with 2 ml of plating medium (2 mM L-glutamine (Gibco), 0.45% glucose (Sigma□Aldrich) ...
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bioRxiv - Genetics 2024Quote: ... 55 mM 2-mercaptoethanol or 2) CTS OpTmizer T cell Expansion medium (ThermoFisher #A1048501) supplemented with 5% FBS ...
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bioRxiv - Biophysics 2021Quote: ... and HA-mUNC79 plasmids or their various mutants (in a modified BacMam expression vector 27) at a ratio of 2:1:1:1 using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 24 hours before recording ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for overnight with primary antibody at 4 degrees and further incubated with secondary antibodies (1:500) for 1 hour followed by 4′,6-diamidino-2-phenylindole (DAPI) or phalloidin 488 (1:400, Thermo Fisher, A12379) staining ...
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bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
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bioRxiv - Physiology 2023Quote: ... Cells were grown on DMEM/Hams F12 with 2% fetal bovine serum and standard cell culture hormones (Dexamethasone 1 1 mM; mM, T3 1 μM, ITS 100X Gibco cat # 41400045) on 6-well ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated B cells were activated at 1 x 106 cells mL-1 for 2 days in IMDM medium (ThermoFisher) with 10% fetal bovine serum ...
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bioRxiv - Genomics 2020Quote: ... 0.5-1×106 proliferating TILs were seeded with 100×106 normal donor peripheral blood mononuclear cells irradiated with 5,000 rad in a 1:1 mixture of TIL media and AIM-2 media (ThermoFisher), supplemented with 30 ng/mL OKT3 (Miltenyi Biotec ...
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bioRxiv - Genomics 2021Quote: ... After 2 days of transduction hNGN2-eGFP media was replaced with 1:1 ratio of Neurobasal A (ThermoFisher, 10888022) and DMEM/F12 medium containing GlutaMAX ...
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bioRxiv - Biochemistry 2020Quote: ... air-drying overnight and incubating with 2 μM TOTO-1 iodide (1 mM solution in DMSO, Thermo Fisher Scientific) for 15 min.
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bioRxiv - Microbiology 2021Quote: ... Previously pelleted spheroplasts were resuspended in 1 mL 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer solution (Gibco) and 100 μl were collected as whole spheroplasts ...
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bioRxiv - Microbiology 2020Quote: ... The monolayers were then overlaid with 1 to 2 ml of a mixture of 1 part 0.6% agarose (Invitrogen) and 1 part complete Eagle Minimal Essential Medium (VWR ...
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bioRxiv - Microbiology 2021Quote: ... 800 µl of isolation medium (DMEM, supplemented with 2% FBS, 1% penicillin-streptomycin and 1% amphotericin B, ThermoFisher Scientific) was added to each well ...
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bioRxiv - Physiology 2020Quote: ... the medium was switched to DMEM/F12 (1:1) plus 2% New Zealand fetal bovine serum (Thermo Fisher Scientific), hormones ...
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bioRxiv - Immunology 2021Quote: ... at 1:0.5:2 molar ratio using Lipofectamine™ 2000 (0.6 ul per 1 ug DNA; Invitrogen™, 11668019); growth medium was refreshed 16 h after transfection ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The tumor was excised and was washed in homogenization buffer (0.25 M sucrose [Merck, CAS 57–50–1], 10 mM HEPES, 1 mM EDTA, 2% antibiotic and antimycotic [ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... for 1 h at room temperature and stained for 10 min with 4,6-diamidino-2-phenylindole (DAPI; 1 mg/ml stock solution diluted 1:50,000 in PBS; Thermo Fisher) at room temperature with at least four PBS washes in-between ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged with 80-90% confluence at a split ratio of 1:2-1:4 using TrypLE (Gibco) for 8 min at 37°C ...
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bioRxiv - Developmental Biology 2023Quote: ... washed 1 x 30 min at room temperature in PBSTE-1% BSA supplemented with 2% phalloidin-AF488 (Invitrogen A12379), washed 4 x 30 min at room temperature in PBSTE-1% BSA and washed 2×30’ at room temperature in PBSTE ...
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bioRxiv - Molecular Biology 2023Quote: ... Inoculated cells were incubated for 1 h at 37°C and then overlaid with a 1:1 mixture of 1.2% Oxoid agar and 2× DMEM (Gibco) with 10% (vol/vol ...
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bioRxiv - Neuroscience 2024Quote: ... pCMVR8.74 and pLVX-EF1α-mitoSRAI-IRES-Puro at a 1:1:2 molar mass ratio using Lipofectamine 3000 (Invitrogen). The next day ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Neuroscience 2024Quote: ... The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Immunology 2022Quote: ... resuspended in 1 mL nuclei wash buffer (2% BSA and 0.2LULul−1 RNase inhibitor (Thermo Fisher, cat. no. AM2694) in 1X PBS ...
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bioRxiv - Neuroscience 2023Quote: ... p21) and pAAV target plasmid in a 4:1:1:2 molar ratio by use of Lipofectamine 2000 (ThermoFisher) according to company protocol ...
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bioRxiv - Immunology 2022Quote: ... OT-I T-cells (∼400,000 in 1 mL growth media) were loaded with 1 μM Fura-2 AM (F1221, ThermoFisher) for 40 min RT ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1-2 h at room temperature (1:1000) for viral titration or an anti-GFP antibody (A11122, Invitrogen) for measurement of oxGFP-positive cells ...
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bioRxiv - Bioengineering 2020Quote: ... chips were washed with 100 ul PBS per channel and stained with the following secondary antibodies diluted 1: 100 in 5% BSA in PBS for 2 hours at room temperature: goat anti-rat Alexa Fluor 488 (Thermo Fisher Scientific A11006), donkey anti-rabbit Alexa Fluor 647 (Thermo Fisher Scientific A31573) ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then incubated in secondary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Alexa 488 anti-Chicken IgY (Invitrogen A11039; 1:400); Atto 647N anti-mouse IgG (Rockland 610-156-121 ...
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bioRxiv - Immunology 2022Quote: ... cells (1.5-2×105 cells/mL) were cultured in complete IMDM ((IMDM [Life Technologies], 10% FBS [26140079, Life Technologies], 1% Penicillin-Streptomycin [Life Technologies] ...
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bioRxiv - Neuroscience 2024Quote: ... The sections were again re-blocked with 5% NGS for 2 hr (second serum blocking) and subsequently stained with rat anti-GFAP (1:500; Invitrogen Cat. #13-0300), and rabbit anti-Megf10 (1 ...
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bioRxiv - Cancer Biology 2024Quote: ... EDTA was added to the samples at a final concentration of 5 mM and the suspension was diluted 1:5 in collection buffer (2 % heat-inactivated fetal bovine serum, Life Technologies; 26140079; 25 mM HEPES, Life Technologies; 15630056) in PBS to reduce the collagenase activity ...
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bioRxiv - Synthetic Biology 2024Quote: ... were mixed at a 17:2:1 molar ratio (85%, 10%, 5%) and transfected using lipofectamine 2000 (Thermo Fisher Scientific; Cat. No. 11668027) following manufacturer protocol ...
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mTOR inhibition enhances delivery and activity of antisense oligonucleotides in uveal melanoma cellsbioRxiv - Cancer Biology 2021Quote: ... 2 mM L-glutamine (Gibco) and 100 IU/ml penicillin/streptomycin (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... N-2 supplement (Gibco, 17502048), B27 supplement minus vitamin A (Gibco ...
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bioRxiv - Bioengineering 2021Quote: ... + 2% human serum albumin (ThermoFisher) + 5 μM ROCKi (Tocris) ...