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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor (AF) 647-conjugated streptavidin (1:300 diluted from 2 mg ml−1 stock solution, Invitrogen S32357) was added ...
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bioRxiv - Developmental Biology 2021Quote: ... Islets were handpicked twice and then incubated in regular islet media (RPMI 1640, supplemented with 5 mM glucose, 10% fetal bovine serum, 2 mM glutamine, 1% antibiotics (Gibco, Gaithersburg, MD)) at 37°C for 3 hrs or ...
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bioRxiv - Microbiology 2020Quote: ... cells or tissue explants were incubated with virus (MOI 0.05-5) for 1-2 hr or overnight respectively at 37°C using fresh media supplemented with fetal bovine serum (Thermo Fisher Scientific). For co-infection assays ...
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bioRxiv - Microbiology 2020Quote: TSA assays were performed by mixing 5 μL protein solution at 2 mg/mL with 5 μL SYPRO®Orange (1:50 diluted from 5000x stock solution, Invitrogen, USA), 5 μl of 8-fold buffer (1200 mM NaCl and 400 mM Tris pH 8) ...
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bioRxiv - Bioengineering 2020Quote: The rADSCs of the 4th passage were seeded on a six-well culture plate at a density of 5 ×105 cell/ml with 1 ml of neurosphere medium containing serum-free DMEM/F12 with 2 % B27 supplement (Thermo Fisher, USA) and 20 ng/ml of basic fibroblast growth factor (bFGF ...
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bioRxiv - Bioengineering 2020Quote: ... 30 and 60 min after induction of neutralization of Col 1 and enzymatic gelation (n=2-5 samples/group) of freshly prepared materials with a Multiskan™ GO Microplate Spectrometer (Thermo Scientific). The following solutions were analyzed ...
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bioRxiv - Immunology 2020Quote: ... cells were resuspended at 5×106 cells/mL in 1 mM MgCl2/PBS and treated with 2 mM DSG cross-linker (Thermo Fisher Scientific) at room temperature for 30 min on a rotator ...
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bioRxiv - Immunology 2023Quote: ... Duplicate wells of 200,000 CFSE-stained cells per sample were then incubated for 91 hours at 37°C (5% CO2) at a 1:2 bead-cell ratio with Human T-Activator CD3/CD28 DynaBeadsTM (Thermo Fisher, #11161D) and 1 U/mL of recombinant human IL-2 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: The RBDs of G614, Alpha, Beta, and Omicron (BA.1, BA.2, BA.4/5) variants were ordered as GeneString from GeneArt (Thermo Fisher Scientific). All sequences of the RBD (aa 319-541 in GenBank ...
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bioRxiv - Physiology 2023Quote: ... and incubated for a period of 1 h in a dark room in 2 ml of extracellular solution containing 5 µM fluo- 4 AM (Thermo Fisher Scientific) or 7 µM Corona Green AM (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... three T175 flasks of Ishikawa cells at 60-70% confluence were supplemented with 1 µM 5-ethynyl-2’-deoxyuridine (EdU, Thermo Fisher Scientific) and incubated overnight to allow EdU incorporation into the DNA ...
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bioRxiv - Neuroscience 2023Quote: ... the fish were paralyzed before being mounted in agarose by bathing them for 2-5 min in a solution of 1 mg/mL α-bungarotoxin (Thermofisher Scientific) in E3 medium ...
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bioRxiv - Immunology 2024Quote: ... Cell concentrations were assessed by acquiring 30 μL of resuspended cells diluted 1:5 in PBS-4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific) without any prior washing steps ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... containing 2% heat-inactivated autologous plasma and 2 mM L-glutamine (Life Technologies), unless otherwise stated ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Neuroscience 2022Quote: ... GlutaMAX and FGF-2 (2 ng/ml) (all reagents from Thermo Fisher Scientific) in 24-well plates for 2 weeks on coverslips coated with poly-D-lysine (100 µg/ml) ...
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bioRxiv - Microbiology 2021Quote: ... 2 min) to nitrocellulose membranes using an iBlot 2 Dry Blotting System (Invitrogen). Membranes were briefly washed in PBS-T (0.1% Tween-20/PBS) ...
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bioRxiv - Synthetic Biology 2020Quote: ... at 2 mA cm−2 in a semi-dry electroblotter (Thermo Fisher Scientific) for 45 min using 1 × TBE buffer ...
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bioRxiv - Microbiology 2021Quote: ... Gibco Hepes (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, 0.05 mM; Invitrogen), ROCK Inhibitor Y-27632 (1 mM ...
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bioRxiv - Cell Biology 2020Quote: ... and 30 U/ml of interleukin-2 (IL-2; Thermo Fisher Scientific, PHC0026) at 37°C ...
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bioRxiv - Microbiology 2021Quote: ... adding 2 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) and 1 μM QUMA-1 23 to the final wash ...
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bioRxiv - Immunology 2021Quote: ... IL-2 ELISA was performed using IL-2 Mouse Uncoated ELISA Kit (Invitrogen) following manufacturer’s protocol.
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bioRxiv - Systems Biology 2023Quote: ... 2 µL Invitrogen TURBO DNase and 2 µL SUPERase•In RNase Inhibitor (Invitrogen). After digest ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Myocytes were then loaded with Fura-2 acetoxymethyl ester (Fura-2 AM; Invitrogen) as described previously (Batiste et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM N-2-hydroxyethyl piperazine-N-2-ethane sulfonic acid (HEPES; Gibco, 15630080), and cOmplete ...
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bioRxiv - Neuroscience 2024Quote: ... 150uL of Tyrode’s containing 2 uM Fura 2-AM (Invitrogen F1221, Lot 2559176)/ Pluronic F-127 (Invitrogen P3000MP ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Neuroscience 2024Quote: ... they were loaded with 2 µM Fura-2-AM (Invitrogen/Thermo Fisher Scientific) dissolved in normal Ca2+-buffer (150 mM NaCl ...
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bioRxiv - Immunology 2022Quote: ... 2 h goat anti-rabbit IgG F(ab’)2-AF488 secondary antibody (Invitrogen), 15 min DAPI (1:1000) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 2 μM Fura-2 AM (Thermo Fisher Scientific, F1225) for 30 min at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... 2 mM glutamine and 2 mM HEPES (Thermo Fisher Scientific, Waltham, MA, USA). Cell culture medium was supplemented with 1.5 μg mL−1 recombinant ApoE (Sigma) ...
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bioRxiv - Bioengineering 2024Quote: ... 10 mM N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES, Gibco 15630080) and 1 % penicillin-streptomycin (PS ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10% glycerol (2 mM 2-mercaptoethanol (BME) and protease inhibitor tablet (ThermoFisher Scientific) added immediately before use) ...
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bioRxiv - Cell Biology 2021Quote: ... followed by an anti-mouse secondary antibody (V5: 1 hr, RT, 1/500; Invitrogen, Cat #A21200; FLAG: 2 hr, RT, 1:200; Invitrogen, #A11004) according to the manufacturer instructions ...
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bioRxiv - Microbiology 2023Quote: ... Resuspended bacteria were then lysed (2 × 108 total CFU) by a 1:1 addition of 1% dodecyltrimethylammonium bromide (DTAB) (AC409310250; Fisher Scientific) for 5 min with agitation ...
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bioRxiv - Microbiology 2024Quote: ... 2% B27, 1% N2, 1% MEM non-essential amino acids, 1% penicillin/streptomycin and 0.1% β-mercaptoethanol, all from ThermoFisher Scientific) supplemented with dual SMAD inhibitors (10 µM SB-431542 and 100 nM LDN-193189) ...
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bioRxiv - Immunology 2024Quote: ... were coated with 1 µg/mL (full dose, or diluted to 1/2, 1/4, 1/8) of anti-CD3 antibody (16-0032-81, Thermo Fisher) in PBS at 4 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Following 2 weeks of selection in 250 μg ml−1 Hygromycin B (Thermo Fisher) and 4 μg ml−1 Blasticidin (Thermo Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysate equivalent to 1-2 × 105 cells was resolved by SDS-PAGE (Life Technologies) and transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cell Biology 2020Quote: ... followed by staining using 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI; Thermo Scientific) for 5 min ...
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bioRxiv - Cell Biology 2020Quote: ... and F(ab’)2 goat anti-mouse Alexa Fluor 633 (Molecular Probes, 1:1000).
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bioRxiv - Genetics 2021Quote: ... Primary antibodies used were: 1:1000 mouse anti-SARS/SARS-CoV-2 N (ThermoFisher Scientific; ...
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bioRxiv - Molecular Biology 2021Quote: ... Preamplified products were treated with 4µl of 1:2 diluted ExoSAP-it (Affymetrix Inc.) and incubated for 30 min at 30°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and the fluorescent dye 4’,6-diamidino-2-phenylindole (DAPI; 1:1,000, Molecular Probes), respectively.
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bioRxiv - Developmental Biology 2020Quote: ... and 4′,6-diamidino-2-phenylindole (DAPI: nuclear counterstain; 1:1000; ThermoFisher, Waltham, MA).
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bioRxiv - Neuroscience 2021Quote: ... THP-1 macrophages were labeled with 2 μM CellTracker Green CMFDA dye (Invitrogen, C2925) in HBSS containing Ca++ and Mg++ (Gibco ...
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bioRxiv - Neuroscience 2021Quote: ... Brain slices were incubated with 4’,6-diaminodino-2-phenylindole (DAPI, Invitrogen, 1:1000) for 15 min ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (1:106, 62248, Invitrogen) for 10 min at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were serum-starved for 1 hour in 2 mL of Opti-MEM (Gibco). Cells were subsequently incubated with ligand and test compounds (1 ...