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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... USA) at 95°C for 5 min and then mixed with 2μL of 5mg/mL Truecut Cas9 protein (Thermo Fisher Scientific, USA) for 10 min at 37°C in a total volume of 50μL ...
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bioRxiv - Neuroscience 2019Quote: ... Solubilized material was centrifuged at 20,000 g for 10 minutes at 4°C and the supernatant was precleared with protein A/G sepharose beads (Thermo Fisher Scientific) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... per sample were reduced with DTT and denatured at 70°C for 10 min prior to loading onto Novex NuPAGE 10% Bis-Tris Protein gels (Invitrogen, Cat. # NP0315BOX). The gels were stained overnight with Novex Colloidal Blue Staining kit (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates were cleared by centrifugation at 12000 rpm for 5 min at 4°C and protein extracts were assayed for firefly and Renilla luciferase activity sequentially in a 96-well luminometry plate (Thermo Fisher Scientific) using a microplate luminometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... for 14-17 h at 4°C followed by the incubation with 100 μl of Dynabeads Protein A + G (2:1 A:G proportion, Invitrogen, 10001D and 10004D) for 2 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... IP were performed by incubation of lysates 4 h at 4 °C on a rotating wheel with either 50 µL of DynaBeads M-280 protein A magnetic beads (Thermo Fisher Scientific), pre-loaded with 8 µg of rabbit control (Dako ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell lysates containing 1 mg of protein were incubated with anti-HA antibody (1:100) for 1 h at 4 °C followed by incubation with Protein G Dynabeads (Thermo Fisher Scientific) for 1 h at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... The chromatin was incubated with antibodies at 4 °C overnight and then added to 30 μl of protein G-Dyna beads (Thermo Fisher Scientific) for an additional 2 hours with constant rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... incubating at 95°C for 5 min and then mixed with 2μL of 5mg/mL Truecut Cas9 protein (Thermo Fisher Scientific, USA) for 10 min at 37° ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary (4°C overnight) and secondary antibodies (1 h, room temperature) dilutions and proteins were visualized with enhanced chemiluminescence (ECL) (Pierce; Thermo Fisher Scientific). For loading control (Supplementary figures 3b and 3k) ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and used to amplify cDNA corresponding to the N and C termini of the spike protein and the plasmid backbone using Platinum SuperFi II DNA Polymerase (Thermofisher Cat# 12368010) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of lysates were incubated with anti-53BP1 for 2 hours at 4°C and subsequently with Protein A magnetic beads (Thermo Fisher Scientific) for 1 hour at 4 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... protein lysates (see section ‘Generation of C. elegans protein extracts’) were supplemented with LDS Sample Buffer (NuPAGE LDS sample buffer, Thermo Fisher Scientific) with 1 mM dithiothreitol (DTT) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein lysates were pre-cleared 1h at 4°C on a rotating wheel with 50μl of previously washed Dynabeads Protein G (Invitrogen, Thermo Fisher Scientific), to avoid aspecific bindings ...
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bioRxiv - Neuroscience 2022Quote: ... Cell debris was cleared by centrifugation at 1000×g for 15 min at 4°C and protein concentration was determined by bicinchoninic acid (BCA) assay (ThermoFisher Scientific #23225). Protein lysates were resolved on Bolt™ 4-12% Bis-Tris Plus gels (ThermoFisher Scientific #NW04125BOX ...
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bioRxiv - Biochemistry 2022Quote: ... which were supplemented with additional 800 μL of RIPA buffer and incubated overnight at 4°C with 15 μL of antibody-coupled protein G Dynabeads (Thermo Fisher Scientific). Before adding the cleared chromatin ...
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bioRxiv - Cell Biology 2023Quote: ... Extracts were then centrifuged at 10,000g for 10 min at 4°C and the protein concentration of the soluble fraction determined using the Bicinchoninic Acid (BCA) Assay (Thermo Fisher Scientific). Samples were denatured in 4X Laemmli sample buffer (BioRad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled at 80 °C for 10 min and proteins separated using precast NuPAGE 4–12 % Bis-Tris gels (Thermo Fisher Scientific, NP0322PK2) at 80 V for ca ...
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bioRxiv - Neuroscience 2024Quote: ... Joachim Herz) for 2 h at 4°C followed by overnight incubation with 20 μL of protein G Ultralink resin (ThermoFisher Cat# 53128). The resins were washed with immunoprecipitation buffer and precipitated proteins were eluted with reducing SDS sample buffer ...
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts were clarified by centrifugation at 1,500x g for 5 min at 4 °C and then protein concentrations in the supernatant were determined using the bicinchoninic acid (BCA) assay (ThermoFisher Scientific #23227). Detergent-extracted brain homogenates were diluted to a concentration of 5 mg/mL in 1X detergent buffer and then incubated on ice for 20 min ...
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bioRxiv - Neuroscience 2024Quote: ... 1h at RT and subsequently incubated overnight at 4°C in primary antibody solution using Rabbit anti-Green-Fluorescent-Protein (GFP; 1:1000 ThermoFisher Scientific, A6455). The following day sections were rinsed three times in PBTriton 0.5% and incubated overnight at 4°C in secondary antibody solution using Goat anti-Rabbit-Oregon Green 488 (1:500 Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... and then re-frozen slowly at −80°C and finally re-thawed at 4°C before determination of protein concentration with a Pierce BCA assay against bovine serum albumin (BSA) as a standard (Thermo Fisher Scientific). Proteins were separated by denaturing SDS-PAGE (10 - 15% (w/v ...
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bioRxiv - Physiology 2022Quote: ... Samples were centrifuged for 20 minutes (12,000 rpm at 4°C) and the protein concentration of each supernatant was determined using the Pierce BCA assay (Thermo Fisher, UK). Proteins were denatured by 2-mercaptoethanol at 95°C and separated using SDS-PAGE in MOPS running buffer on a NuPAGE Bis-Tris 4-12% gel (ThermoFisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 21130 g for 10 min at 4°C and quantified for protein concentration using an albumin standard (Thermo Fisher 23209) and Bradford reagent (Bio-Rad 5000006) ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated at 4 °C overnight with primary antibody rabbit anti-Myosin VI (1:400 Proteus Bioscience) and mouse anti-green fluorescent protein (Invitrogen, cat. A11120). The tissues were incubated with 1:800 diluted secondary antibodies for 2 hrs at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 5-10 µg of total protein was heated at 70°C for 10 min and then resolved on a 4–12% NuPAGE gel (Thermo Fisher Scientific) by SDS-PAGE ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained with primary antibodies overnight at 4°C targeting the following proteins: MAP2 (PA1-10005, ThermoFisher Scientific, 1:5000), AT8 (MN1020 ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 30 minutes at 4°C before sonication and protein quantification was measured using BCA assay (Pierce™, ThermoFisher Scientific, Waltham, MA). 20 μg protein was separated on SDS-PAGE (4-15% Criterion Precast Gel ...
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bioRxiv - Cancer Biology 2023Quote: ... spun at 13,000 rpm for 30 minutes at 4°C and supernatant protein concentration determined using the BCA assay kit (#23225; Thermo Fisher Scientific). 30-50 μg aliquots of lysate proteins were resolved on sodium dodecyl sulfate-7.5% polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Biochemistry 2023Quote: ... Lysates were centrifuged at 14,000xg for 15 minutes at 4°C and protein concentrations were determined using a standard Pierce™ bicinchoninic acid (BCA) assay (ThermoFisher, 23225). Lysate (30 µg ...
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bioRxiv - Biophysics 2023Quote: ... 0.5% NP-40 alternative) with protease inhibitors and cleared for 2 hours at 4 °C with magnetic Protein G Dynabeads (Thermo Fisher, #10009D) before overnight immunoprecipitation with 1 ug per 250ug of proteins of either normal serum IgGs or specific antibodies as listed above ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at 4°C before the addition of 25 μL of Pierce Protein A/G Magnetic Beads (Thermo Fisher Scientific) for an additional 2 h under constant rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Insoluble cell components were separated at 20000 × g for 15 min at 4°C and total protein concentration was assessed using BCA assay (Thermo Fisher Scientific). Protein denaturation was performed in SDS sample buffer at 95°C for 10 min.
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bioRxiv - Molecular Biology 2023Quote: ... lysates were incubated for overnight at 4°C with 2 μg of antibodies recognizing MTR4 or IgG control and then incubated for 1 h at 4°C with Dynabeads™ Protein A (ThermoFisher Scientific). After incubation ...
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bioRxiv - Genetics 2024Quote: ... Samples were denatured at 99°C for 5 minutes and separated on a 4-20% tris-glycine protein gel (Thermo Fisher Scientific) at 150 V for 75 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were centrifuged at 15,000 x g at 4°C for 20 min and protein concentration was quantified using bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific). 10-25 µg of proteins were resolved in 8% SDS-PAGE gels before being transferred onto polyvinylidene (PVDF ...
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bioRxiv - Neuroscience 2024Quote: ... Striatal homogenates were immunoprecipitated by end-over mixing for 30 min at 4°C with Dynal protein G magnetic beads (Invitrogen, Carlsbad, CA) pre-coated with 50ug each of two anti-GFP antibodies (clones 19C8 and 19F7 ...
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bioRxiv - Neuroscience 2024Quote: ... 500 μg protein was incubated with rabbit IgG control or rabbit anti-tau antibody overnight at 4°C and pulled down by protein A/G Magnetic Agarose beads (Thermo Scientific, 88802) for 6 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... lysates of larval fat body or transfected S2 cells were incubated overnight at 4°C with protein A Magnetic beads (Thermo Scientific, #2736141) pre-coated with GFP antibody (Invitrogen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... mammalian expression vector with an amino-terminal protein C tag followed with a 3C protease cleavage site and transfected into Expi293 cells (Thermo Fisher Scientific) using FectoPRO (Polyplus-transfection ...
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bioRxiv - Physiology 2024Quote: ... Samples were centrifuged at 13 000 g for 15 min at 4°C and the protein concentration of each sample determined via Pierce Bicinchoninic Acid (BCA) assay (#23225, Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... We incubated the cells (1 million cells / 1 ml HBSS) obtained after digestions with 1 µl Near-IR fluorescent reactive dyeLIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen; Cat: L34961; Ex: 633/635nm; Em: 775nm) for 30 min on ice and stopped the reaction by addition of 1 ml HBSS supplemented with 5% FBS ...
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bioRxiv - Microbiology 2023Quote: ... protein concentrations were determined by Bradford Protein Assay protein using Coomassie protein assay reagent (Thermo Fisher Scientific-Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... The membranes were blocked with StartingBlockTM T20 (TBS) Blocking Buffer (ThermoFisher Scientific, 37543) at RT for 30 min before incubation with anti-RNF8 antibody (Proteintech ...
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bioRxiv - Neuroscience 2021Quote: ... or Alexa647-conjugated secondary antibodies diluted in blocking solution (1:1000, ThermoFisher Scientific). After rinsing in PBTx ...
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bioRxiv - Developmental Biology 2019Quote: ... After hydration slides were blocked using SuperBlock (TBS) blocking buffer (Thermo Fisher Scientific) and incubated with validated primary antibodies (all diluted 1:250 in SuperBlock ...
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bioRxiv - Cell Biology 2019Quote: ... washed with blocking solution and incubated with fluorophore-conjugated secondary antibodies (Molecular Probes) and DAPI in blocking solution (1h) ...
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bioRxiv - Biochemistry 2019Quote: ... Membranes were blocked with TBS Starting Block Blocking Buffer (catalog no. 37542, ThermoFisher) and probed with primary antibodies (1:1,000 dilutions ...