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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 7 2 Benzylamino ethyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... cell density was adjusted to 106 cells/mL (and was never allowed to go over 3×106 cells/mL) and puromycin selection (2 μg/mL, Gibco, A1113803) was started ...
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bioRxiv - Biochemistry 2022Quote: ... Between 2 to 3 million cells were used per electroporation and were resuspended in 90 μl of buffer R (Thermo Fisher). Proteins were spun down at 16700 g for 10 minutes and then diluted with buffer R in a 1:1 ratio ...
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bioRxiv - Genetics 2022Quote: ... primary rat astrocytes were thawed into T75 flasks and fed once every 2-3 days with glial media {(MEM (Thermofisher 11095080) supplemented with 5% FBS (Gemini 900-108) ...
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bioRxiv - Microbiology 2022Quote: Proteins (8 μM final) were incubated with 0.16 mM of (2'-(or-3')-O-(trinitrophenyl) adenosine triphosphate (TNP-ATP) (Thermo Fisher Scientific), in 50 μl of Binding Buffer (10 mM HEPES-KOH (pH 8) ...
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bioRxiv - Microbiology 2022Quote: ... The grids were blotted with force 2 for 3 seconds and plunged into liquid ethane using a Vitrobot (FEI Thermo Fisher). Cryo-EM data were collected with a Titan Krios microscope (FEI ...
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bioRxiv - Molecular Biology 2022Quote: ... 3-4 mg total protein and 2 ug of KIF1C antibody or 10 ug mCherry antibody (Invitrogen mCherry Monoclonal Antibody (16D7)) were used ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA on a C18 Acclaim PepMap100 trap-column (75 μm ID x 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 min at 6 μL/min with 2% ACN ...
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bioRxiv - Neuroscience 2022Quote: OE sections from mice injected with retrograde tracer were stained with Alexa Fluor 555- conjugated streptavidin to detect biotin-labeled dextran (1:500 in phosphate-buffered saline containing 3 % normal donkey serum and 0.3 % Triton-X100 for 2 h at room temperature, S32355, Thermo Fisher Scientific), or GAP43 (see above ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were injected onto an Acclaim PepMap100 C18 trap column (75 μm i.d., 2 cm long, 3 μm, 100 Å, Thermo Scientific) and further separated on an Acclaim PepMap100 C18 analytical column (75 μm i.d. ...
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The effects of caloric restriction on adipose tissue and metabolic health are sex- and age-dependentbioRxiv - Physiology 2023Quote: ... Tissues in formalin were fixed at 4°C for 2-3 days before being washed and stored in DPBS (Life Technologies). Tissues on dry ice were stored at −80°C prior to downstream analysis.
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bioRxiv - Molecular Biology 2022Quote: ... C18, 3 μm, 100A), then separated using analytical column Acclaim PepMap RSLC (75um x25cm, nanoViper, C18, 2 μm, 100A) (Thermo Fisher). The flow rate was 300 nL/min ...
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bioRxiv - Microbiology 2023Quote: ... Samples were injected and concentrated on a trap column (PepMap100 C18, 3 μm, 100 Å, 75 μm i.d.×2 cm, Thermo Scientific) equilibrated with 0.05% trifluoroacetic acid in water ...
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bioRxiv - Neuroscience 2023Quote: ... the cell pellet was then resuspended in enzyme assay buffer again and sonicated at 3-second intervals for 2 minutes at 50% amplitude (Cat# FB120220, Fisher Scientific). The cell lysate was centrifuged at 1000 x g at 4°C for 10 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were typically passaged every 2-3 days by treating the cultures with 0.05% Trypsin-EDTA (Thermo Fisher Scientific, cat# 15400054) for 8-10 min at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptide mixtures were injected in 0.1% TFA into a C18 Acclaim PepMap100 trap-column (75 μm ID × 2 cm, 3 μm, 100Å, Thermo Fisher Scientific) for 3 minutes at 5 μL/min with 2% ACN ...
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bioRxiv - Plant Biology 2023Quote: Qdot-labeled Cel7A was prepared by mixing 3 nM biotinylated Cel7A with 2 nM Qdot 655 (Thermo Scientific; catalog number: Q10123MP) in 50 mM sodium acetate ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Peptides were loaded first on a Trap column (Acclaim PepMap 100 3 µm C18 column of 2 cm length and 75 µm inner diameter, ThermoFisher Scientific) and then injected on an analytical column (EasySpray 15 cm column 2 µm 100 Å C18 column with 75 µm inner diameter ...
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Pan-tissue mitochondrial phenotyping reveals lower OXPHOS expression and function across tumor typesbioRxiv - Biochemistry 2023Quote: ... of 3 to 30% acetonitrile using a 2□μm EASY-Spray PepMap RSLC C18 75□μm × 250□mm column (Thermo Fisher) with a column temperature of 55□°C ...
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bioRxiv - Biochemistry 2023Quote: ... High-resolution micrographs for single-particle reconstructions (Figure 2 and 3) were collected on a Titan Krios G4 (Thermo Fisher Scientific), which was operated at 300 kV acceleration voltage ...
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bioRxiv - Cancer Biology 2023Quote: ... The tumors were placed into a chilled conical tube containing 2-3 mL of TrypLE Express Enzyme (Gibco; cat. no. 1260413) and transported on ice to a biosafety hood ...
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bioRxiv - Neuroscience 2024Quote: ... Each sample was loaded onto an Acclaim PepMap trap column (75 µm × 2 cm, 3 µm, C18, 100 Å, Thermo Scientific) and then separated on a nanoEase BEH C18 column (150 µm × 100 mm ...
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bioRxiv - Neuroscience 2024Quote: ... Control 1 (C1) and 2 (C2) were derived by StemBANCC from an unaffected volunteer and control 3 (C3) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... cells were passaged every 2–3 days by washing twice with phosphate-buffered saline (PBS) and dissociation with 0.25% Trypsin (Life Technologies, 25200056).
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bioRxiv - Cell Biology 2023Quote: ... slides were rehydrated with 25mM HEPES (Sytox Green) or dPBS (Hoechst, TOTO-3) for 2 minutes followed by incubation in 1µM Sytox Green (Invitrogen, Waltham, MA) in 25mM HEPES ...
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bioRxiv - Immunology 2024Quote: ... We electroporated 3 μg of ODNs or gDNA into 2 × 106 HEK293 and 293A cells by Neon Transfection System (Thermo Fisher) based on the manufacturer’s introductions.
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bioRxiv - Molecular Biology 2024Quote: ... Fractionated samples and bead-enriched samples were individually loaded onto a C18 trap column (3 μm, 75μm × 2 cm, Thermo Fisher Scientific) connected in-line to a C18 analytical column (2 μm ...
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bioRxiv - Molecular Biology 2024Quote: Protein concentrations from fractions 2 and 3 of fed human milk and digesta were determined using a Micro BCATM kit (Pierce, Thermo Fisher), following manufacturer instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Recipient cells were incubated with either PKH67 dye-labelled or fluorescent-labelled sEVs for 2-3 days in media supplemented with 5% exosome-depleted FBS (Gibco, USA). Cells were imaged with time-lapse microscopy or washed with PBS ...
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bioRxiv - Cancer Biology 2024Quote: For acute versus chronic proliferation measurements total cell number for acute and chronic was counted at day 0 and every 2-3 days until day 10 using Countess automated cell counter (Thermo Fisher). For Incucyte® proliferation assay plastic flat bottom 96 well plates were coated with 5 μg/mL human fibronectin in PBS for 1h in 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... either for 2-3 cm or ran completely and stained with colloidal coomassie dye G-250 (Gel Code Blue Stain Reagent, Thermo Scientific). The lane was cut into bands ...
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Plant Biology 2020Quote: ... Data acquisition and quantification was performed with Chromeleon 7 (Thermo Scientific).
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bioRxiv - Biophysics 2021Quote: ... cells were harvested at day 7 using trypsin-EDTA (Fisher Scientific) and centrifugation (320g ...
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bioRxiv - Cell Biology 2020Quote: ... on a QuantStudio 7 Flex real-time PCR detector (Thermo Fisher). Relative mRNA levels were normalized to those of GAPDH mRNA in each reaction and undetermined raw Ct values were set to 40 for analysis purposes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... on a Viia 7 Real-Time PCR system (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... 7 µL of molecular grade water (Fisher Scientific UK Ltd, UK) and 2 μL of DNA template at the original sample concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). All primers were used at 10 μM and sequences can be found in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). Each biological replicate was measured in technical duplicates ...
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bioRxiv - Plant Biology 2019Quote: ... in a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). Ct values for the gene of interest are expressed relative to that Ct value found for primers targeted to AT1G13320 ...
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bioRxiv - Immunology 2020Quote: ... and the ViiA 7 Real-time PCR system (Thermo Fisher Scientific). Primers used in this study are listed in Suppl ...
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bioRxiv - Biochemistry 2019Quote: ... The coding region for MCM3-7 were cloned into pFastbac (ThermoFisher). MCM3 was cloned with an N-terminal FLAG tag (DYKDDDDK ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 7 µl of 100X Halt protease inhibitor (Thermo Fisher Scientific). After 15 min of thawing and resuspension ...
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bioRxiv - Physiology 2019Quote: ... QuantStudio 7 Flex Real time PCR system (Applied Biosystems Life Technologies)
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bioRxiv - Physiology 2019Quote: ... QuantStudio 7 Flex Real time PCR system (Applied Biosystems Life Technologies)
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bioRxiv - Bioengineering 2021Quote: ... in a ViiA 7 Real-Time PCR instrument (Thermo Fisher Scientific). The sequences of all used RT-qPCR primers are listed in Table 2 ...
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bioRxiv - Immunology 2021Quote: ... on a ViiA™ 7 Instrument (Applied Biosystems, Thermo Fisher Scientific).
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bioRxiv - Immunology 2021Quote: ... on a ViiA™ 7 Instrument (Applied Biosystems, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: ... using a QuantStudio 7 Flex Real Time PCR System (Applied Biosystems). Primers are listed in Supplementary Table 1.
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bioRxiv - Bioengineering 2020Quote: 293T and Huh-7 cells were cultured in DMEM (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco ...