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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 7 2 Benzylamino ethyl 3 7 dihydro 1 3 dimethyl 1H purine 2 6 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Five microliters were loaded onto an Acclaim PepMap™ precolumn (75 μm × 2 cm, 3 μm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nL/min on a PepMap™ RSLC C18 Easy-Spray column (75 μm × 50 cm ...
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bioRxiv - Biochemistry 2023Quote: ... In between wash steps the beads were incubated for 3 min on a magnetic stand (DynaMag 2, DYNAL®, Invitrogen). Then ...
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bioRxiv - Cell Biology 2023Quote: ... C3H female mouse in origin) were cultured every 2-3 days using 0.25% Trypsin-EDTA and maintained in DMEM (GIBCO, 11965118) supplemented with 20% iFBS and 1% Penicillin/Streptomycin ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 min) and suspended in 50 µl of TSB-FBS supplemented with 2 U of DNase I (Thermo Fisher Scientific). After 5 min incubation at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 min) and suspended in 30 µl of TSB-FBS supplemented with 2 U of DNase I (Thermo Fisher Scientific). After 5 min incubation at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... Washes of 3 x 1ml PGAS and 2 x 1ml PBS followed by 1ml PBS containing 1μg/ml Hoechst 33342 (Life Technologies) and finally 2 washes in dH2O prior to mounting on glass slides with Mowiol 4-88 (Polysciences Inc.) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Libraries were prepared for long-read sequencing by digesting 2 µg of a level 3 plasmid library using Esp3I (Thermofisher) and purifying linearized fragments using a 0.5x volume of magnetic beads (Omega Bio-Tek ...
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bioRxiv - Genomics 2023Quote: ... For the construction of the RNH2A KO clones, RNASEH2A (Chr19, exon 2) gRNA (5’-TAACAGATGGCGTAGACCAT-3’) was cloned into GeneArtTM CRISPR Nuclease Vector with OFP reporter (Invitrogen) following manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The isolated cells were expanded in tissue culture flasks for 2-3 days in proliferation medium containing DMEM/F-12 media (Gibco), penicillin-streptomycin (Gibco) ...
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bioRxiv - Neuroscience 2023Quote: ... Ten microliters of each sample were loaded onto a trap column (100 mm i.d. x 2 cm; Acclaim PepMap C18, 3 mm, 100A; ThermoFisher 164564) in 0.1% formic acid in water ...
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bioRxiv - Bioengineering 2023Quote: ... cells in each well were firstly washed with flow cytometry staining buffer: 3% FCS with 2 mM EDTA (15575020; Invitrogen) in PBS− ...
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bioRxiv - Biochemistry 2023Quote: ... Each sample of peptides was loaded onto a C18 trap column (75 μm ID × 2 cm, 3 μm, Thermo Scientific) and then separated on a C18 analytical column (75 μm ID×50 cm ...
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bioRxiv - Cell Biology 2023Quote: ... induction medium was replaced every 2-3 days by mature medium containing StemPro-34 SF medium (Gibco, Thermo Fisher Scientific), GlutaMAX (10 μl/ml ...
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bioRxiv - Plant Biology 2023Quote: ... PI staining seedlings were washed for 2-3 min in water and transferred to a chambered cover glass (Thermo Scientific), and imaged either using Confocal laser scanning (CLSM ...
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bioRxiv - Immunology 2023Quote: ... The membrane was then washed 3 times with TBS-T and 2 times in TBS and developed using SuperSignal West Pico PLUS Chemiluminescence reagent (ThermoFisher). The resulting membrane was imaged on a BioRad ChemiDoc system and visualized using Image Lab software (BioRad) ...
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bioRxiv - Molecular Biology 2023Quote: ... hESCs were differentiated into cardiac mesoderm by culturing for 3 days in RPMI B27-medium (RPMI 1640 GlutaMAX + 2% B27 supplement minus insulin (ThermoFisher), 200 μM L-ascorbic acid (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... induction medium was replaced every 2-3 days by mature medium containing StemPro-34 SF medium (Gibco, Thermo Fisher Scientific), GlutaMAX (10 μl/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... MEFs and HeLa) or 2 µL of Lipofectamine 2000 (Calu-3 and VeroE6) according to the manufacturer’s recommendation (ThermoFisher and Invitrogen, respectively). For co-transfections ...
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bioRxiv - Bioengineering 2024Quote: ... and fresh media was added and incubated for a further 24 hours before performing the viability assay using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific). The media was replaced with fresh cell culture media containing MTT (0.25 mg/mL ...
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bioRxiv - Developmental Biology 2024Quote: ... cell vials were thawed 2-3 min in a 37°C water bath and transferred in T25 cell culture flasks (Gibco) with 5ml of hASC culture medium containing Minimum Essential Mediun (αMEM ...
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bioRxiv - Bioengineering 2022Quote: ... cells were rinsed in DPBS and incubated with 2 μg/ml DAPI (4′,6-diamidino-2-phenylindole, 62248; ThermoFisher) and 1:500 Alexa Fluor Plus 647 conjugated goat anti-rabbit secondary antibodies (A32733 ...
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Cancer Biology 2021Quote: ... and TO-PRO-3 (1:3000; Thermo Fisher) for fluorescent labelling of living and dead cells (‘Imaging medium’) ...
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bioRxiv - Bioengineering 2019Quote: ... TOPRO-3 (1:1000, in PBS) (Invitrogen, USA) was used for 15 min at room temperature to stain the nuclei.
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bioRxiv - Genetics 2021Quote: ... 8ul of 1:3 Vectashield (Thermo Fisher Scientific) DAPI:PBS was added to samples and allowed to incubate for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... 1× 10−3 М glutamine (Gibco, Cat:25030149) and 2 mg/ml gentamicin (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... in 1:3 ratio using lipofectamine (Life Technologies) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... TO-PRO-3 (Thermo Fisher T3605, 1:10,000) to label dead cells ...
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bioRxiv - Neuroscience 2021Quote: ... the DNA dye TOPRO-3 (1:1000, Invitrogen), diluted in a blocking buffer for 2 h at 24 °C ...
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bioRxiv - Cell Biology 2021Quote: ... pan-cytokeratin (ThermoFisher, 3-9003-82, 1/50), pSmad1/5/8 (Merck ...
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bioRxiv - Molecular Biology 2022Quote: ... then washed 3 times with 1×PBS (Invitrogen). Roots were then squashed under coverslips onto slides that had been pre-treated with 3-Aminopropylthiethoxysilane (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2022Quote: ... 3) donkey anti-rabbit 488 (1:250, Invitrogen). To visualize immunofluorescence ...
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bioRxiv - Neuroscience 2023Quote: ... anti-V5 (Invitrogen, #R96025, 1:800, 3 days), anti-cFOS (Abcam ...
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bioRxiv - Developmental Biology 2019Quote: Stock solutions of 5-ethynyl-2’-deoxyuridine (EdU) dissolved in dimethyl sulfoxide were prepared per manufacturer’s instructions (Thermo Fisher). Axolotls (3-6cm tail to snout ...
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bioRxiv - Molecular Biology 2022Quote: ... Cultures were passaged every 5 to 7 days with collagenase type IV (Invitrogen; 1 mg/mL). The identities of all parental hESC and hiPSC lines were confirmed by DNA fingerprinting and all cell lines were regularly tested to exclude mycoplasma contaminations using a PCR-based assay ...
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bioRxiv - Microbiology 2021Quote: ... 1% Penicillin/Streptomycin and 7% of heat-inactivated fetal bovine serum (FBS) (all from ThermoFisher Scientific). VeroE6/TMPRSS2 cells (NIBSC 100978 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 minute at 60°C)] on a QuantStudio 5 or QuantStudio 7 qPCR machine (Applied Biosystems). For qPCR assessments ...
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bioRxiv - Immunology 2024Quote: ... Anti-CD49-PerCPefluor710 (TS2/7) and anti-PD-1-PeCy7 (eBioJ105) were supplied by Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using ‘SnakeSkin’ dialysis tubing with a 7 kDa molecular weight cut-off (ThermoFisher). His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Integrin αV was stained with a commercial antibody (ThermoFisher, USA, clone RMV-7, 1:1000 diluted). Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech ...
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bioRxiv - Synthetic Biology 2023Quote: ... Huh-7 and TCMK-1 cells were maintained in high glucose DMEM media (Gibco, 41966-029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and then 1 h at 4°C at 7-10 rpm with dynabeads protein A (Invitrogen), equilibrated with the EB ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 3 mins at 90C and separated on a 6% denaturing PAGE gel (Invitrogen). The RNA was then transferred to a HyBond-N+ nylon membrane (GE Life Sciences ...
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bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... and Nuclear DNA was counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher). Alternatively ...
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bioRxiv - Biophysics 2020Quote: ... Nuclei were detected by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:200, ThermoFisher Scientific, USA) for 10 minutes at RT ...
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bioRxiv - Neuroscience 2020Quote: ... sections were counterstained with 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, D3571, Molecular Probes, dilution 1:1000), re-washed ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with 4’,6-diamino-2-phenylindole dihydrochloride (DAPI, 1:10000 in PBS, Thermo Scientific) and mounted with a cover glass using Fluoromount (Diagnostic BioSystems ...