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Citations for Thermo Fisher :
1851 - 1900 of 10000+ citations for 4 Chloro 6 fluorobenzene 1 3 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The C2C12 cells were transfected with 2 μg plasmids (L-Cas9n-EGFP:R-Cas9n-puro=3:1) by electroporation (1650 v/10 ms/3 pulses) with the Neon Transfection System Kit (Thermo Fisher Scientific). The cells were seeded overnight to 24-well plates containing an antibiotic-free complete mediums ...
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bioRxiv - Microbiology 2024Quote: ... 1 μg of plasmid and 3 μL of Lipofectamine 2000 (m/v = 1:3) were diluted in 0.1 mL of Opti-MEM (Gibco, 31985070, Gaithersburg, MD). After 5 min of incubation ...
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bioRxiv - Microbiology 2024Quote: ... was added at a 1:2000 dilutions for 1 h at 37 °C, followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was lawned with goat anti-human IgG Fc (50 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... and activated with a mixture of 400 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and 100 mM N-hydroxysuccinimide (Thermo Fisher Scientific). The activated chip was coupled directly to mAbs diluted to 10 µg/mL in 10 mM sodium acetate (pH 4.5 ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Microbiology 2023Quote: ... The chip was activated with a freshly prepared solution of 130 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (Pierce PG82079) and 33 mM N-hydroxysulfosuccinimide (Sulfo-NHS) (ThermoFisher Scientific 24510) in 0.1 M MES pH 5.5 using the SFC ...
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bioRxiv - Developmental Biology 2022Quote: ... Maturation of released oocytes was induced by incubating for 1h at 16C in 3 μM 1-Methyladenine (Fisher Scientific, 5142-22-3). All embryos were raised in 0.22 μm filtered sea water (FSW ...
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bioRxiv - Immunology 2022Quote: ... targeting SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA using RNAiMAX (ThermoFisher, 13778-075). 24 h after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... 5’-GAGACCCUAUCCGUGAUUAtt-3’ and antisense: 5’- UAAUCACGGAUAGGGUCUCtt-3’ (Silencer Select, rat negative control #1; scrambled siRNA and all siRNAs were from Ambion, Life Technologies). Transfection complexes were prepared in accordance with the instructions provided by the manufacturer and added to 2×105 cells seeded per well in 24-well plates.
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bioRxiv - Immunology 2024Quote: ... and activated by adding 50 mg/ml sulfo-NHS (N-hydroxysulfosuccinimide) and EDC [1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride] (Thermo Fisher Scientific). Each bead region was then incubated with 6.25 μg of the respective antigen yielding a final concentration of 6.25μg antigen/1 × 10^6 beads in coupling buffer [0.5 M 2-(N-morpholino ...
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bioRxiv - Microbiology 2024Quote: ... Serum samples were assayed at 3-fold dilutions starting at a 1:3 dilution in Blocker Casein in PBS (Thermo Fisher Scientific) diluent ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by transfer to a positively charged nylon membrane and then crosslinked with EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) at 60°C for 1–2 hours and prehybridized with ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen, cat # AM8670). The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen ...
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bioRxiv - Bioengineering 2021Quote: ... 50 μg of pre-aliquoted Tandem Mass Tag 6-plex (TMT-6, Thermo Scientific) was resuspended in 20 μL anhydrous acetonitrile ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma IL-6 was measured by immunoassay (ProQuantum Human IL-6 Immunoassay Kit, ThermoFisher) and run on the ABI 7300 (Applied Biosystems ...
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bioRxiv - Bioengineering 2023Quote: FsC[Fe3+] was labelled by 6-carboxyfluorescein (6-FAM) (Thermo Fisher Scientific, Waltham, USA) to indicate the targeting of FsC[Fe3+] to C ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit anti-Claudin-4 (1:200, Thermo Fisher Scientific, 36-4800), rabbit anti-Hopx (1:100 ...
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bioRxiv - Bioengineering 2021Quote: ... and ethidium homodimer-1 (4 µM, stains dead cell nuclei, Invitrogen) for later timepoints.
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bioRxiv - Neuroscience 2020Quote: Cells were loaded with 1 μM Fluo-4-AM (Thermo Fisher) in culture medium and incubated at 37 °C and 5% CO2 for 30 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... were used at 4°C overnight: anti-GFP (1:250, Invitrogen), anti-DsRed(RFP ...
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bioRxiv - Microbiology 2020Quote: ... Mouse monoclonal anti-Claudin-4 (1:100, Novex, Life Technologies, USA) and rabbit polyclonal anti-claudin-4 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... a 1:4 dilution of trypsin was used (Gibco, Cat# 15400054) to split the cells into thirds.
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bioRxiv - Bioengineering 2022Quote: ... eBCs were stained with polyclonal Synaptotagmin-4 antibody (1:50; Invitrogen) and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2020Quote: ... 25 mM 4-(2-hydroxyethyl)-1-piperazineeethanesulfonci acid (HEPES; Thermo Fisher), and 1X non-essential amino acids (Gibco ...
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bioRxiv - Bioengineering 2020Quote: dLNs were fixed for 1 hour with 4% paraformaldehyde (Thermofisher Scientific), embedded in Tissue-tek OCT (VWR ...
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bioRxiv - Neuroscience 2021Quote: ... GPC-4 (#13048-1-AP, Thermo Scientific, and #MABN528, Sigma-Aldrich).
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bioRxiv - Cancer Biology 2020Quote: ... overnight at 4 °C and alpha-Tubulin (Invitrogen, 11224-1-AP) for 1-2 hours at room temperature ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Aliquots of 4 µL of 1 mM Mitotracker Green FM (ThermoFisher) stock solution were added to the samples and were kept in the dark at 15°C for 15–20 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200 rabbit anti-FABP-4 (Thermo Fisher Scientific, PA5-30591), 1:200 rabbit anti-Osteocalcin (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... the aliquots were incubated with 4-pentyn-1-o1 (ThermoFisher Scientific) and ADPRC (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2020Quote: ... KP-4 and PANC-1 cells were maintained in DMEM (Gibco). LOX-IMVI cells were maintained in RPMI 1640 medium (Gibco) ...
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bioRxiv - Cancer Biology 2022Quote: ... ki67 (ThermoFisher, Cat. RM-9106; 1:50 overnight at 4 °C), βIII-tubulin (Biolegend ...
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bioRxiv - Neuroscience 2024Quote: ... and mouse anti-HuC/D (Elavl3/4) (1:500, Invitrogen A21271) in PBST overnight at 4 °C in a humidified chamber ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 μL of 1 μM Fluo-4 AM (ThermoFisher Scientific, F14021) in GBO medium was added to each GBO at the ALI surface for at least 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... plus DAPI (Thermo Fisher Scientific, 1:1000 in 4% BSA PBS) at room temperature ...
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bioRxiv - Genomics 2023Quote: ... 1X 4-(2-hydroxyethyl)-1- piperazineethanesulfonic acid (HEPES) (Thermo Fisher Scientific), 1X MEM non- essential amino acids (Thermo Fisher Scientific) ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: Astrocytes were loaded with Fluo-4 AM (1 mM; Molecular Probes) in culture media for 20 min at 37°C followed by 10 min wash in HBSS (Sigma ...
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bioRxiv - Neuroscience 2024Quote: ... 4% Surecast solution (Acrylamide:Bis-acrylamide ratio of 29:1, ThermoFisher #HC2040) with 0.5% VA-044 activator (Wako ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C overnight or 1:500 AlexaFluor-488-streptavidin (Invitrogen) for 48 hours ...
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bioRxiv - Synthetic Biology 2024Quote: ... anti-mouse-IL-4-APC (17-7041-82, Invitrogen, 1:50) or anti-mouse-IL-17-eFluor™ 450 (48-7177-82 ...
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bioRxiv - Immunology 2022Quote: ... The 40 mg/ml 4-HT stock solution was diluted 1:1 in Kolliphor EL (MilliporeSigma) 59.Before injection the solution was further diluted 1:3 in PBS (Gibco) and warmed to 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Biochemistry 2020Quote: ... Insoluble fraction was removed by high speed centrifugation at 4°C (25658 g for 30 minutes, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was filtrated with 0.22 μm filter (Filtropur BT50 0.2 ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (Invitrogen 4’,6-diamidino2-phenylindole). Photomicrographs of immunofluorescence staining were taken using a Leica DMRA fluorescence microscope with a Leica DFC360 FX camera (Leica ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...
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bioRxiv - Immunology 2021Quote: ... bacteria were stained with 2µg/µL of 4’,6-diamidino-2-phenylindole (DAPI) and mounted in ProLong Diamond Antifade (Life Technologies, Eugene, OR, USA; P36961). Image acquisition was performed using a Leica confocal microscope (Leica ...