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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for 4 Chloro 6 fluorobenzene 1 3 diamine since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... Protein lysates were run at 200V for an hour through either a 4-12% Bis-Tris or 3-8% Tris Acetate gel (Invitrogen), and then transferred at 300 mA for one hour in 15% methanol 1x Transfer Buffer on a methanol activated PVDF membrane ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of total cellular proteins fractionated through 3-8% polyacrylamide Tris-acetate or 4-12% polyacrylamide Bis-Tris gels (Invitrogen) were subsequently transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: ... were crossed for 2-3 days and then transferred to fly food made from 0.6 g of Carolina Formula 4-24 (Fisher Scientific) and 2 mL of ddH2O ...
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bioRxiv - Microbiology 2020Quote: ... the same amounts of SOSIP trimer and SOSIP-I53-50NP were loaded on a 4-12% Bis-Tris NuPAGE gel or 3-12% Bis-Tris NuPAGE gel (both from Invitrogen), respectively.
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... For differential glucose concentration treatments between day 2 and 3 and between day 3 and 4, N2B27 was made using DMEM/F12 without glucose (L0091-500, Biowest) and Neurobasal without glucose (A2477501, ThermoFisher). For 1X and 3X glucose concentrations ...
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bioRxiv - Immunology 2023Quote: Following euthanasia, adult zebrafish heads (15 wpf, N=3) were fixed in a solution of 4% methanol-free formaldehyde (Thermofisher) in 60 mM HEPES buffer (pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell bodies were removed by centrifugation (1,150 g, 3 min, 4°C; Sorvall Legend XTR, Thermo Fisher Scientific). A sucrose cushion (10 ML of 25% sucrose in HMS ...
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bioRxiv - Molecular Biology 2023Quote: ... 5% CO2 in a humidified incubator and were passaged every 3-4 days using 0.05% Trypsin-EDTA (ThermoFisher Scientific, 25300). Stable U2OS-derived cell lines ...
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bioRxiv - Immunology 2023Quote: ... The cells were then blocked at 4℃ for 15 mins in 200 µl CyFACS buffer containing 3 µl rat serum (Invitrogen), 3 µl mouse serum (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (human female in origin) were cultured every 3-4 days using 0.05% Trypsin EDTA and maintained in DMEM (Gibco, 11965118) supplemented with 10% heat inactivated fetal bovine serum (iFBS ...
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bioRxiv - Neuroscience 2022Quote: ... slices for optical recordings were incubated for 30 minutes in oxygenated Krebs solution added to a 3% Di-4-ANEPPS (Invitrogen) stock solution and mixed with an equal volume of fetal bovine serum (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... mRNA samples of 4 ng were subjected to DNaseI treatment and 3’ dephosphorylation using FastAP Thermosensitive Alkaline Phosphatase (Thermo Scientific) and T4 PNK (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The clarified lysate was transferred to a 15 mL column containing 3 mL Ni-NTA Agarose affinity resin equilibrated with buffer A at 4 °C (Invitrogen). The column was washed using a gradient of Buffer A containing 20 mM ...
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bioRxiv - Genomics 2024Quote: ... and transfer plasmids were transfected at a mass ratio of 2:3:4 with Lipofectamine 3000 (Thermo Fisher Scientific L3000001) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were resolved on precast NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). Samples were transferred to 0.45 µm nitrocellulose membrane (0.9 A ...
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bioRxiv - Molecular Biology 2022Quote: ... The Gibson reaction mix was transformed into Mach1 competent cells (4 μl Gibson into 40 μl cells, 288 reactions in 3×96-well plates) following manufacture’s protocol (Invitrogen C862003). After transformation ...
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bioRxiv - Biophysics 2022Quote: ... N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (NBD-PE) was purchased from ThermoFisher (Waltham, MA). Cholesterol Oxidase from Streptomyces was purchased from MP Biomedicals (Santa Ana ...
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bioRxiv - Cell Biology 2022Quote: ... at 180V on precast either NuPAGE Novex 4-12% Bis-Tris Gels in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen), or NuPAGE™ 3-8% Tris-Acetate gels in NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... The cells were passaged every 3-4 days after they achieved confluency of 80-90% using Trypsin 0.25% EDTA (Life Technologies).
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Neuroscience 2022Quote: ... the cell cultures were washed 3 times with 37 °C DPBS+ and fixed using 4% w/v paraformaldehyde (PFA; Affymetrix) in PBS for 2.5 h ...
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bioRxiv - Genetics 2022Quote: ... LC-MS/MS based proteomics datasets (4 groups × 3 replicates) were then generated in a lab (code: NVG) using Q Exactive HF-X mass spectrometer (ThermoFisher). To eliminate confounding factors such as experimental sample-processing order with sample group ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were treated with either DMSO or 5 μM GolgiTracker ((BODIPY™ FL C5-Ceramide (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Pentanoyl)Sphingosine)) (Invitrogen. Cat# D3521) for 30 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Equal amounts of total cell lysates were loaded to 4–12% Bis-Tris or 3–8% Tris-Acetate gels (Invitrogen) and transferred to 0.2 µm PVDF membranes via wet tank or semi-dry method (specified in the supplementary information) ...
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bioRxiv - Biochemistry 2023Quote: ... Proteins were resolved on NuPAGE 3-8% (wt/vol) Tris-acetate or 4-12% (wt/vol) Bis-Tris gels (Invitrogen) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... 16 mL of HMA-10 buffer was added and the preparation was put on a shaking rocker for one hour at 4 °C with 3 units/mL Rnase-free Dnase (Ambion) added ...
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bioRxiv - Cell Biology 2024Quote: ... islets from 3-4-week-old control and β-LonP1KO mice were handpicked and then dispersed with trypsin (Thermo Scientific). Islet cells were counted and incubated with adenovirus expressing an empty vector control (Ad-RIP2 EV ...
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bioRxiv - Pathology 2024Quote: ... retinas were post-fixed in 4% PFA for 3 minutes at room temperature before mounting in Prolong Gold antifade medium (Invitrogen). Images were acquired using a Zeiss LSM710 and Zeiss LSM880 confocal microscope ...
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bioRxiv - Cell Biology 2024Quote: ... 30 to 50 μg of protein extracts were resolved by SDS-polyacrylamide gel electrophoresis using 4–12% NuPAGE Bis-Tris gradient gel or 3–8% Tris-acetate gel and run (Invitrogen) and MOPS or Tris-acetate running buffer (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... The 10µl of cells suspension infected with virus was plate micromass cultures (3-4 spots per 35mm culture dish, NUNC #150318) at a density of 2 x 107 cells/ml ...
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bioRxiv - Cell Biology 2024Quote: ... red blood cells were removed by adding 1 ml ACK lysis buffer (150 mM NH4Cl and 10 mM KHCO3) and incubated on ice for 3 minutes before washing with HBSS containing 4% fetal bovine serum (HI FBS, Gibco). During the optimization of the protocol outlined above ...
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bioRxiv - Genetics 2024Quote: ... Proteins were loaded and run on a 4-12% Bis-Tris pre-cast gel or a 3-8% Tris-Acetate pre-cast gel (Invitrogen) and were electrophoretically transferred to a PVDF membrane (Millipore) ...
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bioRxiv - Cell Biology 2024Quote: ... or BODIPY 558/568 C12 (C12) (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (ThermoFisher, #D3835) were complexed with fatty acid-free bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2021Quote: ... HEK293T cells were transfected with (pRMB EDEN15) and BASU plasmids (6:1 ratio) using Lipofectamine 2000 (1:1 ratio, Life Technologies, California, USA). 40 hours post-transfection ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... pH 7.4) for 10 min and later incubated with 4 uM fluo-4/AM (1 mM, Molecular probes, #F14201) and 0.02% pluronic F-127 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... 6 hours prior to collection organoids were incubated with 1 μM EdU (Thermo fisher, #C10636).
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with DAPI (4ʹ,6-diamidino-2-phenylindol) 1:10,000 (Thermo Fisher Scientific). Images were recorded using an Axioimager 2 Microscope with cooled CCD camera (SPOT Imaging Solutions ...
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bioRxiv - Neuroscience 2022Quote: ... using samples diluted to 1:1000 while interleukin (IL)-6 (Thermo Fisher Scientific, Cat. #BMS625) was evaluated using samples diluted 1:2 ...
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bioRxiv - Immunology 2022Quote: ... in 6-well plates and cultured in 1 ml/well RPMI-1640 medium (Thermo Fisher) containing 4% B-27 (Life Technologies) ...
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bioRxiv - Neuroscience 2022Quote: ... Fresh media was added and passaged 1:6 using the StemPro EZpassage tool (Life Technologies) as per the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... The GLT-1 encoding sequence was subcloned into pcDNA™6 V5 Myc (Thermo Scientific).
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bioRxiv - Systems Biology 2023Quote: Approximately 1*10^6 UWB1.289 were lysed in 650 µl Tri reagent (Thermo Fisher, #AM9738) and bead milled with the following settings ...
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bioRxiv - Cell Biology 2022Quote: ... Suspension cells (THP-1) were first seeded for 6 h on poly-D-lysine (Gibco)-coated wells before starting the assay ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Systems Biology 2022Quote: ... with the addition of 6 μl ERCC Spike-In Control Mix 1 (Ambion Thermo Fisher) diluted 1:10,000 ...
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bioRxiv - Immunology 2024Quote: ... 1 µM or 0.4 µM of 5(6)-carboxyfluorescein diacetate succinimidyl ester (CFDA-SE; Invitrogen), which is metabolized within cells to carboxyfluorescein succinimidyl ester (CFSE) ...
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bioRxiv - Microbiology 2024Quote: ... 6 pmol of siRNA (siCHC-1: Silencer Select s475, siCHC-2: s477 Thermo Fisher 4390824) were mixed with 100 µl of OptiMEM reduced serum media (Thermo Fisher 31985070 ...