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Citations for Thermo Fisher :
1801 - 1850 of 10000+ citations for 6 CHLORO 4 METHYL 3 PHENYLCOUMARIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... using a QuantStudio 6 (Thermo Fisher Scientific). Relative LepR mRNA expression levels were determined using the 2−ΔΔCT method normalized to β-Actin mRNA expression levels ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (ThermoFisher, #D1306) at a concentration of 10 µg/ml in PBS / 3.0%BSA for 15 minutes then rinsed 3x 5 minutes in distilled water ...
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bioRxiv - Immunology 2023Quote: ... with a QuantStudio 6 Pro (Applied Biosystems). For murine Slc2a5 ...
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bioRxiv - Cell Biology 2023Quote: ... puromycin (6 μg/ml; Thermo Fisher Scientific) was added to the select transduced cells ...
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bioRxiv - Immunology 2023Quote: ... 6 U/ml DNase I (ThermoFisher Scientific) and 60 U/ml hyaluronidase (Millipore Sigma ...
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bioRxiv - Genetics 2022Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). The Ct values were analyzed by the relative fraction of input method.
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bioRxiv - Genetics 2022Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). Samples were analyzed using a two-step amplification and melt curves were obtained after 40 cycles ...
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bioRxiv - Developmental Biology 2023Quote: ... using QuantStudio 6 Flex system (Applied Biosystems). Gapdh was used as endogenous control and data analyzed with ΔΔCt method ...
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bioRxiv - Immunology 2023Quote: ... no UNG (Life Technologies, Quant Studio 6). Reactions were carried out for 40 cycles ...
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bioRxiv - Physiology 2024Quote: ... on a QuantStudio 6 Pro (Applied Biosystems). Probes included ...
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bioRxiv - Microbiology 2023Quote: ... 6’-diamidino-2-phenylindole (DAPI; Life Technologies) diluted 1:10000 in PBS ...
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bioRxiv - Microbiology 2023Quote: ... Novex TBE 6 or 10% gels (Invitrogen) were pre-run in 0.5x TBE running buffer (pH ∼8.4 +/- 0.5) ...
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bioRxiv - Genomics 2023Quote: ... using a QuantStudio 6 FLEX machine (Thermofisher). See Supplementary Data for primers ...
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bioRxiv - Immunology 2024Quote: ... on the QuantStudio 6 platform (ThermoFisher Scientific). Ct values of Ifng were normalized to the Ct values for Gapdh ...
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bioRxiv - Immunology 2023Quote: ... 6 μl DNase I (Thermo Fisher Scientifiс) was added for additional 15 min ...
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bioRxiv - Immunology 2024Quote: ... 6 μl DNase I (Thermo Fisher Scientifiс) was added for additional 15 min ...
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bioRxiv - Immunology 2019Quote: ... 5’-CCCTACTGTATCCTCATG-3’/5’-CTTACCTCCTCTTCAATAGC-3’ PRKDC: 5’-GGGGCATTTCCGGGTCCGGG-3’/5’-TGCCCTGCCCCCCACTCTGC-3’ Amplicons were cloned using the Zero Blunt TOPO PCR Cloning kit (ThermoFisher), prepared as plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... Guide RNA primer sets A (KS40 5’-ACCGACCACAGAAACTGCGACTAG -3’ and KS41 5’-AACCTAGTCGCAGTTTCTGTGGTC-3’) and B (KS42 5’-CCGTCCCACTGGCACCGCTTTATG-3’ and KS43 5’-AAACATAAAGCGGTGCCAGTGGGA-3’) were phosphorylated with T4 polynucleotide kinase (ThermoFisher) at 37°C for 30 min and annealed by cooling down from 85°C to 25°C at 0.1°C/sec ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 4 μM Mag-Fluo-4 (ThermoFisher Scientific) and 1 μM F-127 (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... and/or ToPro-3-3 (1 μM; Thermo-Fisher Scientific, Waltham, MA) for 10 minutes at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) was purchased from Life Technologies/Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... #3: 5′-GAAUAUUGAACUGGAAGCAGCACAU-3′) were purchased as Stealth RNAi siRNAs from Invitrogen. The target sequences were designed using the Block-iT RNAi Designer tool (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... 20 mL of 3 mM of 3-deoxyadenosine (cordycepin; Thermo Fisher Scientific) was added to the buffer to obtain a final concentration of 0.6 mM (150 mg/L) ...
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bioRxiv - Biochemistry 2023Quote: ... 5’-UUCUCCGAACGUGUCACGUTT-3’ and 5’-ACGUGACACGUUCGGAGAATT-3’ using Lipofectamine RNAiMIX reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... 300,000/6-well) and IDC (5,000/96-well; 100,000/6-well) cells were seeded in regular (Thermo Fisher Scientific) or ULA (Corning Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were forward transfected in 6-well dishes by using 6 μl of FuGENE® HD (PRE2311, Fisher Scientific) with 3 μg of sgRNA ...
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2020Quote: Cells in the 6-well plates were transfected with 2μg plasmid of AMPKα or OGG1 via 6 μl Lipofectamine 2000 (Invitrogen) for 6 hours following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The Cas9-sgRNA-containing plasmid and donor vector were co-transfected at a molar ratio of 1:6 into XX mESCs cells on a 6-well plate using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher). Transfected cells were passaged at different densities into three Petri dishes with feeders ...
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bioRxiv - Biochemistry 2021Quote: ... 6% native PAGE gels were prepared by first making a gel solution containing 6% acrylamide (29:1) (Fisher Scientific).15 This solution was then degassed under vacuum and mixing ...
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bioRxiv - Microbiology 2020Quote: ... and resuspended in 0.1 ml of PBS supplemented with the lipophilic styryl membrane dye N-(3-triethylammoniumprpyl)-4-(p-diethylaminophenyl-hexatrienyl) pyridinium dibromide (FM4-64, Molecular Probes, Invitrogen; 10 μg.ml-1) (Pogliano et al. ...
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bioRxiv - Neuroscience 2022Quote: ... brain slices were washed (5 min at RT x 3 times) in PBS and incubated (overnight at 4°C) with streptavidin-Alexa 488 (ThermoFisher Scientific, S-11223, 1:500) in PBS-triton 0.05% ...
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bioRxiv - Physiology 2022Quote: ... The samples were then mixed with 1 mL of a 3:13 solution of Ehrlich’s reagent (1.5 g of 4-[dimethylamino] benzaldehyde [ThermoFisher]; 5 mL ethanol; 337 µL sulfuric acid) to isopropanol and incubated for 30 min at 58°C ...
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bioRxiv - Neuroscience 2020Quote: ... was added prior to sonication on the lowest power setting (Output power: 3-4 Watts; 3x for 10 sec on, 30 sec off; Fisher Scientific Sonic Dismembrator Model 100). The samples were incubated with 150 units micrococcal nuclease (Cell Signaling ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA). Viral supernatants were collected 48 hours post-transfection and filtered through a 0.45 μm Nalgene syringe filter SFCA (Whatman ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 3, 5, 8, and 24 h incubation and centrifuged (21500 xg, 4 °C, 20 min) (Thermo Scientific SL 8R Centrifuge, Thermo Fisher Scientific, Darmstadt, Germany). The fluorescence intensity of the supernatant was measured measured (485 nm excitation ...
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bioRxiv - Genomics 2020Quote: ... Day 3 SP34 (Invitrogen) with 5 ng/ml BMP4 ...
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bioRxiv - Neuroscience 2020Quote: ... 3’-Diaminobenzidine (Invitrogen, 750118) as a substrate ...
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bioRxiv - Neuroscience 2020Quote: ... 3 mM DTT (Invitrogen) and 40 units RNAse OUT (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μM MMC (ThermoFisher) for 6 hours ...
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bioRxiv - Cell Biology 2022Quote: A 3% agarose (Invitrogen) gel solution was prepared in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... IL-3 (Life Technologies), Dexamethasone (Sigma) ...
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bioRxiv - Systems Biology 2020Quote: ... QuantStudio 3 qPCR (Thermofisher) with KAPA Library Quantification Kit (Roche) ...
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bioRxiv - Cell Biology 2020Quote: ... SUMO-2/3 (Invitrogen); β-Catenin (BD Transduction Laboratories) ...
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bioRxiv - Immunology 2022Quote: ... and 3% FBS (Invitrogen). Epidermal sheets were separated from the dermis after incubation for 45 min at 37°C in 2.4 mg/ml of Dispase and 3% FBS and the epidermis was further digested for 30 min in PBS containing 1 mg/ml collagenase D ...
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bioRxiv - Neuroscience 2022Quote: ... QuantStudio 3 from ThermoFisher was used ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... on QuantStudio 3 (ThermoFisher) and data were quantified by the 2-ΔΔCT method.
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bioRxiv - Biophysics 2023Quote: ... DiIC18(3) stain (Invitrogen). Transferrin from Human Serum ...