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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Heme oxygenase 1 HO 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The primary antibody was added at a ratio of 1:100 and incubated for at least 1 h at room temperature while the secondary antibody (1:300 Invitrogen) was allowed to incubate with the sample for 30 min at room temperature ...
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bioRxiv - Developmental Biology 2021Quote: ... 4° C following by re-suspending the cells in 0.5-1 of ml cell isolation media (2% FCS, DPBS:HBSS=1:1 and 1mM SYTOX Blue Dead Cell stain (ThermoFisher Scientific). Cells were imaged before FACS and after FACS to confirm successful purification of GFP+ cells.
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bioRxiv - Microbiology 2021Quote: ... 6-diamidino-2-phenylindole (DAPI) at 1 μg mL−1 concentration and 50 μL of Film Tracer 1-43 membrane marker (Invitrogen) at 1 μg mL-1 concentration in DMSO ...
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bioRxiv - Developmental Biology 2022Quote: ... N2B27 (DMEM/F12 [Gibco 11320-074], and Neurobasal in 1:1 ratio [Gibco 21103-049], with 1:200 N2 Supplement [Gibco 17502-048] ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant after enzymatic reaction was diluted (1:300) in methanol:water (1:1) and injected into a Dionex UltiMate 3000 LC system (Thermo Scientific) with a ZIC-pHILIC (150 mm x 4.6 mm ...
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bioRxiv - Cancer Biology 2022Quote: 22RV1 shSCRM and 22RV1 shMALAT1 cells (1 × 104) were plated in a serum-free stem cell medium consisting of DMEM-F12 (1:1, Invitrogen), 1X B27 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30, 1:250, Invitrogen; Rabbit anti-MyosinVIIa ...
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bioRxiv - Molecular Biology 2019Quote: ... Antibody-bound chromatin was captured with magnetic beads for 1 h at 4°C (50 μl 1:1 mixture of Dynabeads Protein A and Dynabeads Protein G, Invitrogen). DNA from both input and ChIP samples was then extracted using phenol/chloroform ...
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bioRxiv - Developmental Biology 2019Quote: ... 1% low melting point (LMP) agarose (ThermoFischer) was prepared as 1:1 v/v ratio of 1 × PBS and Schneider’s medium (ThermoFisher 21720024) then pipetted onto a 3 cm Petri dish (MatTek ...
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bioRxiv - Cell Biology 2019Quote: ... The parasites were subjected to incubation with secondary antibody (1:400 anti-rabbit Alexa 568, 1:400 anti-rabbit Alexa 488, 1:250 anti-mouse Alexa 594 from Invitrogen) for 1.5 hours followed by three washes with 1X PBS for 10 minutes each ...
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bioRxiv - Developmental Biology 2020Quote: ... After washing with PBS guts were incubated with secondary antibodies (1:500 Goat anti-MouseAlexa647 [Invitrogen]; 1:500 Goat anti-RatAlexa647 [Invitrogen]; 1:500 Goat anti-RabbitAlexa647 [Invitrogen]) and DAPI (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were washed again in PBS and incubated for 2 h at room temperature in secondary antibody in carrier solution (donkey anti-goat 647, 1:1000, ThermoFisher; donkey anti-rabbit 405, 1:1000, Abcam; donkey anti-rabbit 555, 1:1000, ThermoFisher). After four final washes ...
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bioRxiv - Cancer Biology 2021Quote: ... blocked with 3% BSA in PBS with 0.1% Tween20 for 1 hour and immunostained with rabbit anti-nestin (1:200, PA-1-86334; Invitrogen, USA), rabbit SOX2 (1:200 ...
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bioRxiv - Physiology 2021Quote: ... We performed the assay using a modified 1-plex protocol for Fast Blue Detection using Type 1 Mouse AGER probe and Type 1 Mouse GAPDH probe (Affymetrix). The sections were counterstained with Nuclear Fast Red ...
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bioRxiv - Developmental Biology 2019Quote: The following primary antibodies were used: anti-ZO-1 (Zymed, #40-2200, 1:100 or Invitrogen, #33-9100, 1:100); anti-N-cadherin (BD Biosciences ...
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bioRxiv - Genomics 2021Quote: ... these droplets were transferred at a density of 6 droplets per 1 well of a 6-well plate into STEP3 medium containing a 1:1 mixture of DMEM/F12 (Invitrogen) and Neurobasal with 0.5X N2 supplement (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... at 1:1000, goat α-rat (AF488, RRID:AB_2534074) at 1:2000, goat α-mouse (AF594, RRID:AB_141372) from Invitrogen (1:2000). Images were quantified using FIJI software (National Institutes of Health ...
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bioRxiv - Cell Biology 2020Quote: ... media was changed gradually into a gradient of (1/4) Neurobasal media (NB: based medium contained 1% Lglutamine, 1% N2 and 2% B27 (all from Invitrogen)) plus reduced gradient of (3/4 ...
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bioRxiv - Microbiology 2020Quote: ... PBMCs were counted and cryopreserved at a concentration of 1×108 cells/ml in 1:1 RPMI and PBMC freezing medium of 85% fetal bovine serum (Gibco) and 15% dimethyl sulfoxide (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were incubated overnight at 40C in 5% NDS/PBS (Nkx2-1 [abcam Cat. Ab227652] 1:500, St18, 1:1000, Ki67 [Invitrogen Cat ...
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bioRxiv - Microbiology 2022Quote: ... mice were given a mixture of antibiotics (ampicillin 1 mg/mL, vancomycin 0.5 mg/mL, neomycin 1 mg/mL, metronidazole 1 mg/mL; Fisher Scientific) in drinking water for 3 consecutive days (Figure 2G).
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bioRxiv - Biochemistry 2022Quote: ... 1:100 dilution of mouse anti- alpha 1 Spectrin (ab11751) and 1:500 dilution of rabbit anti-KCNN4 (76647, Invitrogen). For co- immunostaining experiments with rabbit primary antibodies ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% Fetal Bovine Serum (EuroBio) and 1% Pen/Strep + 1% Sodium Pyruvate + 1% Non-Essential Amino Acids solution (Gibco) at 37°C in 5% CO2 humidified incubators ...
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bioRxiv - Cell Biology 2022Quote: ... anti-HA(C29F4, Cell Signalling Technology,WB,1:1000, IF,1:500),anti-His (MA1-21315, Invitrogen; WB,1:10,000), anti-EEA1 antibody was a kind gift from Prof ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with 1 x PBS with 1% FBS and incubated with 1:2000 Alexa Fluor 488-streptavidin (S32354, Invitrogen) in 1 x PBS with 1% FBS for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... The 40 mg/ml 4-HT stock solution was diluted 1:1 in Kolliphor EL (MilliporeSigma) 59.Before injection the solution was further diluted 1:3 in PBS (Gibco) and warmed to 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were incubated in rabbit anti-RIM 1/2 antibody (1:200) followed by donkey anti-rabbit AF647 antibody (1:500; Invitrogen).
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bioRxiv - Neuroscience 2022Quote: ... On day 1, medium was switched to N2-medium (DMEM/F12 without L-glutamine, 1%, Glutamax, 1% N2 (all from Gibco), 0.3% glucose ...
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bioRxiv - Neuroscience 2024Quote: ... and anti-Iba-1 (1:1000, 019-19741, Wako) were visualized using goat-anti-rabbit Alexa Fluor 555 (1:500, A21428, Invitrogen). Wisteria floribunda lectin (1:1000 ...
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bioRxiv - Pathology 2024Quote: ... Antibodies to TOM70 (14528-1-AP, 1:200, Proteintech; Alexa Fluor 488-conjugated secondary antibody: A-11034, 1:500, Invitrogen), and troponin T (1:100 ...
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bioRxiv - Pathology 2024Quote: ... were stained in LV cryosections of 10 µm using a rabbit antibody to TOM70 (14528-1-AP, 1:200, Proteintech; Alexa Fluor 488-conjugated secondary antibody: A-11034, 1:500, Invitrogen) and a mouse Alexa Fluor 647-conjugated antibody to TnT (#565744 ...
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bioRxiv - Bioengineering 2024Quote: ... Tissue constructs were cultured for three weeks in chondrogenic medium (DMEM supplemented with 1% penicillin-streptomycin-glutamine, 1% HEPES (1M), 1% sodium pyruvate (100 mM), 1% ITS (100x) (Insulin, Transferrin, Selenium) (all from Gibco), 0,47 mg/ml linoleic acid (Sigma) ...
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mTORC1-dependent SOCE activity regulates synaptic gene expression and muscle response to denervationbioRxiv - Physiology 2024Quote: ... 1/250 for IF, 1/1000 for WB), SARAF (PA5-24237, 1/250 for IF, 1/1000 for WB) from Invitrogen; Naked2 (#2073 ...
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Regulation of Diseases-Associated Microglia in the Optic Nerve by Lipoxin B4 and Ocular HypertensionbioRxiv - Molecular Biology 2024Quote: ... Sections were then incubated in primary antibodies (anti-CD74, 1:100, BioLegend; anti-Iba1, 1:100; anti-p-PI3K, 1:100, Invitrogen) dissolved in blocking buffer overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 700 μL complete RPMI media (cRPMI; 10% FBS [Gibco], 1% penicillin/streptomycin [Gibco], 1% sodium pyruvate [Gibco], 1% non-essential amino acids [Gibco] ...
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bioRxiv - Cell Biology 2024Quote: ... plates were prepared with 2 % agar in YPD (1 % yeast extract, 1 % peptone, and 1 % glucose) supplemented with 100 μg/mL zeocin (Invitrogen), 300 μg/mL hygromycin B (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were transfected at a concentration of 106 cells ml-1 with plasmid DNA (1 mg l−1) and 25 kDa linear polyethyleneimine (2 mg l−1) in OptiPRO SFM (Gibco). After 6 d of culture ...
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bioRxiv - Molecular Biology 2023Quote: ... 150 mM NaCl, 1% NP-40, 10% glycerol) and protease inhibitors (1: 1,000 DTT, 1: 100 Halt protease inhibitors (ThermoFisher Scientific), 1 mM PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... Trypsin activity was stopped by diluting 1+1 with a 1 mg/mL solution of soy bean trypsin inhibitor (#10684033 Gibco) in PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... N2B27 (DMEM/F12 [Gibco 11320-074], and Neurobasal in 1:1 ratio [Gibco 21103-049], with 1:200 N2 Supplement [Gibco 17502-048] ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated overnight at 4 °C with primary antibodies diluted in 1% NGS in 1% BSA/PBS at 1:50 for rabbit anti-Cytokeratin-20 (CK20) polyclonal antibody (Invitrogen), or 1:30 for mouse anti-uroplakin-III (UPIII ...
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bioRxiv - Bioengineering 2023Quote: ... Cryosections were blocked with 1% bovine serum albumin in PBS for 1 h at room temperature and incubated with primary antibodies (anti-perilipin 1, PA5-72921, 1:200, ThermoFisher, Waltham ...
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bioRxiv - Cell Biology 2023Quote: ... HCT116 cells were transfected with 2μg of total DNA of px330 cas9 and homology arms plasmids (mAID-mClover-HygR and mAID-mClover-NeoR for AID cell line and APEX2-mClover-HygR and APEX2-mClover-NeoR for APEX cell line) in 1:1:1 ratio and selected with 100 μg/mL hygromycin B (Invitrogen) and 700μg/ml of Geneticin (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 15% FBS, 1% NEAA, 1 mM sodium pyruvate [Gibco] ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated with anti-CD16/32 (FC receptor block) at 1:100 and LIVE/DEAD Fixable (1:400-1:800; cat no., 15519340; Invitrogen) in 20 μl PBS for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used for immunofluorescence: anti-ACA (1:300; 15-235; Antibodies Inc.) and anti-CENP-A (1:100, MA 1-20832, Thermofisher). The secondary antibodies used were Alexa Fluor® 488 AffiniPure donkey anti-human IgG ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Torics Bioscience) ...
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bioRxiv - Neuroscience 2023Quote: ... six-well plates containing prewarmed neural differentiation medium (NDM; Neurobasal A, 2% B27, 1% N2, 1% Glut Amax, 1% P/S; all from Gibco) supplemented with 20 ng/mL of EGF and FGF2 (both PeproTech ...
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bioRxiv - Neuroscience 2023Quote: ... 47,5% DMEM/F12, 2% B27 w/o Vitamin A, 1% N2 supplement, 1% GlutaMAX, 1% Penicillin/Streptomycin; all from Gibco) supplemented with 10 µM SB-431542 (Tocris Bioscience) ...