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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Heme oxygenase 1 HO 1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... AML12 cells were grown in Dulbecco’s Modified Eagle Medium/F12 (1:1) with 10% FBS and 1% Insulin-Transferrin-Selenium supplement (Gibco) at 37°C with 5% CO2.
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bioRxiv - Microbiology 2020Quote: ... the cells were washed in PBS and incubated for 1-h at RT with 1 mL anti-Early Endosomal Antigen 1 (EEA1) monoclonal antibody (F. 43.1; Invitrogen) diluted 1:200 in blocking buffer ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-©tubulin (mouse monoclonal, MilliporeSigma T6557, 1:200) and mouse anti-ZO-1 (Life Technologies, 1:300). Rabbit anti-Pard3 (rabbit polyclonal ...
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bioRxiv - Microbiology 2022Quote: ... with 7⍰.5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000μg.mL−1 respectively; Life Technologies) and supplemented with 1⍰% non-essential amino acids (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... in serum-free N2B27 medium (DMEM/F12 [Gibco] and Neurobasal [Gibco] 1:1 ratio, 1:100 N2 Supplement [Gibco] ...
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bioRxiv - Developmental Biology 2022Quote: ... Phalloidin (Abcam Phalloidin 405 ab176752 1:1000, Sigma Aldrich Phalloidin 555 P1951 1:400, Invitrogen Phalloidin 647 1:100) and secondary antibodies from Invitrogen ...
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bioRxiv - Microbiology 2022Quote: ... with 7 .5% heat-inactivated fetal calf serum (FCS; Life Technologies with 1% penicillin/streptomycin (PS, 5000U.mL−1 and 5000µg.mL−1 respectively; Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Vpr was probed using Anti-HIV-1 Vpr monoclonal (diluted 1:200) (Cosmo Bio) and Goat anti-Mouse HRP-conjugated (diluted 1:5,000) (Invitrogen) antibodies ...
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bioRxiv - Microbiology 2020Quote: ... followed by 1 h incubation with goat anti-mouse AlexaFluor488 (A21131, Invitrogen; 1:1000 in PBS with 1% BSA). Cell nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Immunology 2020Quote: ... CB (1:1) and buffy coats (1:2) were diluted with sterile 1xPBS (Gibco by Life Technologies, California, USA) and MNCs were isolated by density gradient centrifugation (Biocoll ...
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bioRxiv - Immunology 2020Quote: ... CB (1:1) and buffy coats (1:2) were diluted with sterile 1xPBS (Gibco by Life Technologies, California, USA) and MNCs were isolated by density gradient centrifugation (Biocoll ...
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bioRxiv - Cell Biology 2022Quote: ... antibodies in PBS containing 1% BSA for 1 h and then with Alexa Fluor secondary antibodies (1:1000, Invitrogen) and Hoechst 33258 (1:2000 ...
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bioRxiv - Neuroscience 2022Quote: ... incubated for 1 hr at 37°C with DiI (1:50) and DiO (1:50) (ThermoFisher cat. V2285; V2286), washed ...
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bioRxiv - Cell Biology 2023Quote: ... antibiotics (100 μg ml−1 penicillin, 100 µg ml−1 streptomycin and 0.25 µg ml−1 amphotericin B, Gibco), and 2 mM glutamine ...
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bioRxiv - Cell Biology 2023Quote: ... antibiotics (100 μg ml−1 penicillin, 100 µg ml−1 streptomycin and 0.25 µg ml−1 amphotericin B, Gibco), and 2 mM glutamine ...
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bioRxiv - Microbiology 2023Quote: ... and 1 uL diluted RNAse (1:100-1:6000 dilution from 10 U/uL stock EN0602, Thermo Fisher Scientific) were added to the cellular extracts ...
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bioRxiv - Cell Biology 2023Quote: ... antibiotics (100 μg ml−1 penicillin, 100 µg ml−1 streptomycin and 0.25 µg ml−1 amphotericin B, Gibco), and M-CSF (100ng/ml ...
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740).
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Cas13d-mediated isoform-specific RNA knockdown with a unified computational and experimental toolboxbioRxiv - Genomics 2023Quote: ... we combined 1 µg of total RNA + 2 µl 1:100 ERCC RNA Spike-in Mix #1 (Invitrogen #4456740). Libraries were prepared using the Kapa RNA HyperPrep Kit with RiboErase (Roche #08098131702) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 mM sodium pyruvate and 100 U mL−1 penicillin and 100 µg mL−1 streptomycin (Thermo Fisher Scientific) at 37°C and 5% CO2 in a humidified incubator.
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bioRxiv - Biophysics 2024Quote: ... cells transduced with the pLKO.1 puro backbone or sg-Bag6 1 with Ef1alpha Puro-P2A-BFP(1)(1) backbone were cultured in medium containing 0.5 µg/ml Puromycin (Gibco).
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bioRxiv - Microbiology 2024Quote: ... with 7.5% heat-inactivated Fetal Calf Serum (FCS; Life Technologies with 1% penicillin/streptomycin PS, 5000U.mL−1 and 5000µg.mL−1 respectively (Life Technologies) and supplemented with 1 % non-essential amino acids (Life Technologies) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1% Triton X, 0.5% sodium deoxycholate, 0.1% SDS, 1 mM EDTA, 1× Halt Protease Inhibitor Cocktail from Thermo Scientific, 1x Phosphatase Inhibitor Cocktail from Cell Signaling Technology) ...
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked in 5% milk for 1 hour at RT prior to incubation with primary antibodies (Muc2: 1:1000 from Proteintech, IRE1β: 1:500 and AGR2: 1:1000 from Invitrogen, and Fmo5: 1:1000 from Cell Signalling) overnight at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: ... The cells were then washed three times with PBS containing 1% BSA followed by 1 h incubation with donkey anti-mouse Alexa Fluor 488-conjugated secondary antibody (1:1000, ThermoFisher) at 4°C in the dark ...
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bioRxiv - Cell Biology 2020Quote: ... or on YPD plates (1% yeast extract, 1% peptone and 1% glucose) containing 2% agar supplemented with 100 µg/mL zeocin (Invitrogen), 300 µg/mL hygromycin B (Invitrogen ...
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bioRxiv - Developmental Biology 2021Quote: ... The final pellet was resuspended in advanced DMEM/F12 supplemented with 1% Glutamax/1% HEPES/1% penicillin/streptomycin (all Gibco) and the desired amount of cell suspension mixed with Basement Membrane Extract (Type 2 ...
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bioRxiv - Genomics 2020Quote: ... 100 ng of total RNA spiked with 1 μl of 1:500 diluted ERCC Mix 1 (Life Technologies, Carlsbad, CA) was used to start cDNA synthesis ...
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bioRxiv - Microbiology 2022Quote: ... anti-mouse surface antigen 1 (SAG1) mab DG52 1:500 46 and then with goat anti-rat-Alexa546 1:300 (Invitrogen), goat anti-rat-Alexa375 1:300 (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2019Quote: ... were cultured in (DMEM containing D-glucose [1 g L-1] and sodium pyruvate [110 mg L-1] (Life Technologies), supplemented with penicillinstreptomycin [100 U mL-1] (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... the apical and basolateral sides were gently washed three times with 1xDPBS and the filters were 1% PFA fixed and stained with polyclonal rabbit anti-zona occludens-1 (ZO-1) antibody (Invitrogen, Rockford ...
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bioRxiv - Bioengineering 2020Quote: CellTrace™ prelabeled cells from encapsulations were removed from the well plate surface by a sterile spatula and at least three gels were batched per sample into an enzymatic digestion solution consisting of 1:1 solution of HBSS (calcium and magnesium supplemented) and 1% bovine serum albumin in PBS with 1 µM HEPES (Gibco), 300 U/mL collagenase type IV (Worthington Biochemical) ...
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bioRxiv - Neuroscience 2019Quote: ... and subsequently incubated in secondary antibody (goat anti-chicken 488 1:200, Invitrogen A32931; goat anti-rabbit 647 1:200, Invitrogen A21244; goat anti-rabbit 568 1:200, Invitrogen A11011 ...
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bioRxiv - Biochemistry 2020Quote: ... which consists of DMEM/F12 and Neurobasal medium (1:1) with 1% B27 supplement (Thermo Fisher Scientific, Carlsbad, CA, USA). The medium was changed every five days for 30 days and consisted of the removal of half of the content and addition of this same volume of fresh medium ...
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bioRxiv - Bioengineering 2021Quote: ... and enzymatically digested in a solution consisting of 1:1 solution of HBSS (calcium and magnesium supplemented) and 1% bovine serum albumin in PBS with 1 μM HEPES (Gibco), 300 U/mL collagenase type IV (Worthington Biochemical) ...
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bioRxiv - Cell Biology 2021Quote: ... with 10% FBS (CellMax, Lanzhou Minhai Bio-Engineering, Lanzhou, China) and RPE-1 cells (ATCC, CRL-4000) in DMEM/F12 (1:1) (GIBCO) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2020Quote: ... the inoculum was removed, and 2 mL overlay media was applied (MEM with 10% FBS containing 1% methylcellulose, 1% HEPES, 1% GlutaMAX (Gibco), and 1% Pen/Strep ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202 ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were washed and incubated with fluorescent secondary antibodies diluted in 1% BSA in PBS for 1 h (1:400; anti-mouse-Alexa488, Invitrogen A21202; anti-rabbit-Alexa568, Invitrogen A10042 ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200) was performed for 1 h at 37°C in combination with 1 μM HCS cell mask (Invitrogen; H32720) that stains the entire cell ...
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bioRxiv - Microbiology 2022Quote: ... a-tubulin (1:500, 2144, Cell Signalling), acetyl-a-tubulin (1:500, 1215, Cell Signalling) GAPDH (1:10,000, G9545, Invitrogen, Fisher Scientific) and GAPDH (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... Virus was produced by transfecting all plasmids at a 1:1:1 molar ratio (30 μg total DNA) into HEK293T cells with Lipofectamine3000 (Invitrogen). Viral particles were collected at 72 hours by filtering spent medium through a 0.45 μm cellulose acetate filter (Millipore Sigma) ...
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bioRxiv - Immunology 2022Quote: ... were harvested bilaterally into cold complete RPMI media (cRPMI; 10% FBS [Gibco], 1% penicillin/streptomycin [Gibco], 1% sodium pyruvate [Gibco], 1% non-essential amino acids [Gibco] ...
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bioRxiv - Cell Biology 2022Quote: ... slices were labelled with SPY650-DNA (1:1000, #SC501, Spirochrome) for 1 h and CellTraceTM CFSE (1:1000, #C34554, ThermoFisher) for 30 min ...