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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Cell Biology 2023Quote: Pooled HUVEC sample sets were grown to confluence before treatment with or without recombinant tumour necrosis factor alpha (TNF; 10 ng/mL) (ThermoFisher) in cell culture medium ...
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bioRxiv - Cell Biology 2023Quote: ... while islets were seeded onto the same coverslips coated with recombinant human laminin-521 (Gibco, #A29248, 0.5 μg/cm 2). Cells/islets were allowed to attach overnight ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant bacmid DNA that carried the mini-HA domain was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... High titre (>3 × 108 viral particles per ml) recombinant baculovirus was obtained using the Bac-to-Bac expression system (Invitrogen) in Sf9 (Spodoptera frugiperda ...
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bioRxiv - Molecular Biology 2023Quote: ... Recombinant baculovirus generation and large-scale expression in Sf9 cells were carried out using the Bac-to-Bac™ (Invitrogen) expression system.
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Systems Biology 2023Quote: ... supplemented with 2.5 µg of human recombinant epidermal growth factor (rEGF) and 25 mg of bovine pituitary extract (BPE) (Gibco, 17005042). Cells were passaged at 80% confluency using TrypLE Express Enzyme (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Plant Biology 2023Quote: The 6xHis-NBSNIK1 recombinant plasmid has been previously cloned in pDEST17 via LR-mediated recombination (Gateway Invitrogen Life Technologies, Inc). It harbors the NIK1 kinase domain fused to 6xHis under the control of an isopropyl-β-d-thiogalactopyranoside (IPTG ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Developmental Biology 2023Quote: ... transgenic fragments were further shuffled into the pLenti-Gateway vector via Gateway LR recombinant reaction using Gateway LR Clonase II Enzyme Mix (Invitrogen). In the case of KCNA2 and OVOL1 ...
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bioRxiv - Cell Biology 2023Quote: The baculovirus preparation for recombinant human HAUS complex expression was carried out according to the manufacturer’s protocol (Bac-to-Bac system, Life Technologies) using E ...
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bioRxiv - Cell Biology 2023Quote: Generation of replication-defective recombinant adenovirus expressing N-SREBPs under CMV promoter was performed using ViraPower Adenoviral Expression System (#K4930-00, Invitrogen) as described earlier (54) ...
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bioRxiv - Systems Biology 2023Quote: ... T cells were expanded in RPMI-1640 media supplemented with 10% heat-inactivated FBS (HI-FBS) as well as recombinant human IL-2 (50 U/mL; ThermoFisher) and IL-15 (1 ng/mL ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Recombinant PrP substrates containing the D178N or E200K mutations were created by site-directed mutagenesis with the GeneTailor system (Invitrogen) using the bank vole (BV ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2023Quote: ... and intracellular staining was performed using recombinant hu-mouse anti SeV-NP antibody conjugated with AlexaFluor-647 (1:1000) (Invitrogen) in combination with either anti-Krt5 (1:500 ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of the reverse transcription reaction was used to amplify regions of interest using gene-specific primers in a 50 µL PCR reaction using recombinant Taq DNA Polymerase (Invitrogen) (see Table S2) ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting plasmid was utilized for the generation of recombinant Baculoviruses according to the Bac-to-Bac expression system protocol (Invitrogen). Exponentially growing Sf9 cells (2 x 106 cells/mL in Lonza Insect-XPRESS medium supplemented with 0.1 mM biotin ...
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bioRxiv - Biophysics 2023Quote: ... These plasmids were used for the generation of recombinant baculoviruses following Bac-to-Bac expression system protocols (Invitrogen, Catalog # 10359016).
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bioRxiv - Cancer Biology 2023Quote: 33G needles were filled with Matrigel and L15-BSA with or without (control) addition of 25 nM human recombinant EGF (Invitrogen). Mice were anesthetized using 5% isoflurane and laid on their back ...
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bioRxiv - Neuroscience 2023Quote: ... Each tissue was homogenized in 500 μL of lysis buffer containing 400 units of RNase inhibitor (RNaseOUT Recombinant Ribonuclease Inhibitor, 10777-019, Invitrogen) and 0.1% Triton-X100 in low sucrose buffer (0.32 M sucrose ...
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bioRxiv - Cancer Biology 2023Quote: ... recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Recombinant His-tagged Endocan (R&D) was immobilized on 50 μl of HisPur Ni-NTA Magnetic Beads (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The recombinant plasmid and the empty pBI121 vector underwent digestion using the restriction enzymes XbaI and SacI (Thermo Scientific TM). The purified digested insert and vector ligated using T4 DNA ligase at a 3:1 ratio ...
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bioRxiv - Microbiology 2023Quote: Recombinant baculoviruses (AcNPV) were generated using pFastBac1 AcGFP-JEVcore-FOS and a Bac-to-Bac system (Invitrogen, Carlsbad, CA, USA) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human recombinant epidermal growth factor (EGF 30µg/ml) and bovine pituitary extract (BPE 0.2 ng/ml) (Thermo Fisher Scientific). Primary Epidermal Keratinocytes ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using random hexamer primers (Mycrosynth) with recombinant M-MuLV reverse transcriptase (RevertAid, Thermo-Fisher Scientific, Cat #K1691) and used for quantitation of TTP-bound RNA and total RNA by RT-qPCR with HOT FIREPol EvaGreen qPCR Supermix (Medibena ...
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bioRxiv - Microbiology 2024Quote: ... 1μg recombinant PolyHis-p62 was adsorbed to 5 μl pre-equilibrated packed Ni-NTA resin (Thermo Fisher Scientific, Cat# 88221) via 1 hr ...
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bioRxiv - Microbiology 2024Quote: ... while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen). After digestion ...
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bioRxiv - Immunology 2024Quote: ... or 50 ml of 0.5 mg/ml MSP2 recombinant antigen (McCarthy et al., 2011a) in PBS were coated to 96-well flat bottom MaxiSorb plates (Nunc) overnight at 4°C ...
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bioRxiv - Immunology 2024Quote: Purified recombinant protein vaccines were analyzed on SDS-PAGE by staining with GelCode Blue Safe Protein Stain (Thermo Fisher Scientific). Proteins separated by SDS-PAGE were also transferred onto nitrocellulose membranes for immunoblotting ...
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bioRxiv - Cell Biology 2020Quote: ... rabbit and rat and Alexa Fluor 532 conjugated to antibodies raised against mouse and rabbit (Invitrogen) were used at 1/200 dilution in the saturation media ...
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bioRxiv - Neuroscience 2019Quote: ... Milipore) and a secondary Alexa Fluor 546 goat-anti-rabbit (1:400 anti-rabbit, Molecular probes) using an antibody diluent (Zytomed Systems) ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 680 donkey anti-rabbit or Alexa Fluor 800 donkey anti-rabbit (1:10,000; Invitrogen). To confirm equal protein loading ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit cardiac fibroblasts were treated with rabbit fibroblast media supplemented with either dimethylsulfoxide (DMSO, Thermo Fisher) vehicle or Etoposide (MedChemExpress) ...
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bioRxiv - Cancer Biology 2021Quote: ... and secondary antibody α-rabbit (1:5000, goat anti-rabbit IgG DyLight800 SA5-35571, Invitrogen, USA). Signal detection was performed with the LI-COR Odyssey Infrared Imaging System (LI-COR Bioscience ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... LAMP1 was stained with rabbit anti-LAMP1 and anti-rabbit Alexa Fluor 568 (Thermo Fisher Scientific).
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Bipartite viral RNA genome heterodimerization influences genome packaging and virion thermostabilitybioRxiv - Molecular Biology 2022Quote: ... a rabbit anti-FHV polyclonal antibody and an Alexa Fluor 488 goat anti-rabbit IgG (Invitrogen) were used to probe FHV alpha ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-rabbit-A647 (ref. A31573) and anti-rabbit-A488 (ref. A21206) were from Molecular Probes (Invitrogen). Alexa-conjugated antibodies A568 (ref ...
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bioRxiv - Cell Biology 2024Quote: ... AF488 donkey anti Rabbit IgG and AF647 polyclonal donkey anti-rabbit IgG antibody (all from Invitrogen) were used.
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bioRxiv - Neuroscience 2023Quote: ... tryptophan hydroxylase 2 (primary: rabbit anti-TpH, Novus #NB100-74555; secondary: goat anti-rabbit 546, Invitrogen #A-11035 or goat-anti rabbit 405 ...
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody against rabbit (Alexa-Fluor 488, donkey anti rabbit IgG, 1:1000, Invitrogen (Thermofisher) A21206 ...
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bioRxiv - Neuroscience 2023Quote: ... A secondary antibody against rabbit (Alexa-Fluor 488, donkey anti rabbit IgG, 1:1000, Invitrogen (Thermofisher) A21206 ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 488 goat anti-rabbit and Alexa Fluor 555 goat anti-rabbit (1:500, Invitrogen). After mounting ...
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bioRxiv - Cancer Biology 2021Quote: ... or anti-rabbit (A27034, Life Technologies) secondary antibody at 1:1000 dilution at RT for 1 hour ...