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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Cleaved PARP Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... rabbit anti-FITC (Invitrogen A889), and Mouse anti-Biotin (Invitrogen 03–3700 ...
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bioRxiv - Immunology 2024Quote: ... The native or chimeric 8H11 mAbs were biotinylated in a 20-fold molar excess of EZ-link NHS-PEG4-Biotin (Thermo Fisher Scientific, Waltham, MA, USA) following the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: CHO K1 mAb cell lysates obtained after reducing cell culture temperature were analysed using an Orbitrap Exploris™ 480 mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) online hyphenated to an UltiMate™ 3000 RSLCnano system using an EASY-Spray™ source ...
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bioRxiv - Microbiology 2023Quote: ... at 4°C and incubated with mouse anti-HA mAb clone 12CA5 conjugated and crosslinked to protein A/G magnetic agarose beads (Pierce; Rockford, IL. Thermo Fisher Scientific. Waltham, MA, USA). After rotation at 4°C for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... washed once with PBS and mixed with a total of 10 μg recombinant DNA in electroporation buffer R (Invitrogen). Cells were singularly electroshocked at 1600mV for 20 msec by placing the electroporation tip into the column filled by E2 buffer (following manufacturer specifications) ...
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bioRxiv - Microbiology 2021Quote: The HA pull down was performed using 50μl of the Immobilized Recombinant Protein G Resin (Generon) and specific antibodies against HA (Invitrogen). In order to perform the pull down ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Cancer Biology 2019Quote: ... human recombinant Epidermal Growth Factor 1-53 (EGF 1-53, 5 ng/ml, Thermo Fisher Scientific, catalog #17005-042) and 1% penicillin/streptomycin ...
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bioRxiv - Neuroscience 2019Quote: ... PBMCs were cultured for 9 to 12 days in an erythroblast enrichment medium [38] to expand the erythroblast population and 2.5×105 cells were transduced using recombinant Sendai viral vectors (Cytotune-iPSC 2.0TM, Thermofisher scientific), expressing the four reprogramming factors Oct4 ...
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bioRxiv - Microbiology 2019Quote: The PfCHC-2xFKBP-GFP soluble extract in buffer B was incubated with 4μl recombinant anti-GFP nanobodies covalently coupled to surface-activated Epoxy magnetic beads (Dynabeads M270 Epoxy, ThermoFisher) for 1 hour ...
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bioRxiv - Immunology 2019Quote: ... in OT-1 culture medium supplemented with 100 U/ml mouse recombinant IL-2 (Thermo Fisher Scientific, catalog 14802164) in a 37 °C/5% CO2 incubator ...
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bioRxiv - Systems Biology 2019Quote: Recombinant protein antigens were first labeled with biotin using EZ-Link™ NHS-LC-Biotin reagent (Thermo Fisher, #21366) and then hybridized with the PETAL array ...
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bioRxiv - Immunology 2021Quote: The recombinant chimera CD14-Fc protein was biotinylated using the EZ-Link Sulfo NHS-LC-LC-Biotin kit (Thermofisher) and a 5-fold molar excess of biotin ...
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bioRxiv - Bioengineering 2021Quote: ... and resuspended in B8 media with varying concentrations if iMatrix laminin and/or truncated recombinant human vitronectin (ThermoFisher #A14700). Cells were seeded at 5,000 cells/cm2 ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant proteins were affinity purified by overnight binding to HisPur Cobalt Resin (Thermo Fisher Scientific, U. S. A.) in buffer A (50 mM Tris-HCl (pH 7.6) ...
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bioRxiv - Immunology 2020Quote: ... The vector was linearized with BglII and transformation of yeast (Pichia pastoris) and induction of recombinant protein expression was carried out according to the manufacturer’s instructions (Invitrogen). Recombinant HELMCC protein was purified from yeast culture supernatants by ionexchange chromatography using Ni2+ affinity chromatography (Amersham Biosciences) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were purchased from Kerafast and grown in keratinocyte serum-free media supplemented with bovine pituitary extract and recombinant epidermal growth factor (Gibco, #37010022) with 1% Penicillin-Streptomycin ...
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bioRxiv - Cancer Biology 2021Quote: Recombinant lentiviral and retroviral vectors produced from previous steps were amplified using the GeneJET Plasmid Maxiprep Kit (Thermo Scientific). The 3rd generation lentiviral (pRSV.REV ...
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bioRxiv - Neuroscience 2020Quote: ... Some mice received a mixture of muscimol and Cholera Toxin Subunit B (Recombinant) Alexa Fluor (647 conjugate, Thermo Scientific) to localize site of infusion ...
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bioRxiv - Microbiology 2020Quote: ... Purified soluble recombinant proteins were tested for bacterial endotoxins using the Limulus Amebocyte Lysate (LAL) assay kit (Invitrogen, USA).
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bioRxiv - Cell Biology 2020Quote: ... containing the two recombinant Gateway sites attB1.1 and attbB.1 and subcloned into a pDONR/Zeo Gateway Donor vector (Invitrogen) as the entry clones ...
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bioRxiv - Cell Biology 2021Quote: ... Ift81 and Wdr35 were amplified by PCR with oligos carrying the recombinant sites and cloned into pcDNA3.1(+) vector (Thermofisher) in which a FLAG or HA epiptope was introduced prior to the multicloning site ...
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bioRxiv - Systems Biology 2022Quote: ... and recombinant synthetic genes fused to the GFP-coding sequence at the 3-termini were ordered from GeneArt (ThermoFisher). Genes were further cloned into pDUAL-PuroR lentivectors (Escors et al. ...
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bioRxiv - Biochemistry 2022Quote: ... Pierce™ recombinant protein A/G and clear round-bottom immuno nonsterile 96-well plates were obtained from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The recombinant P0 virus for each gene were generated in ExpiSf9 insect cells following the manufacturer’s recommendations (Thermo Fisher). The amount of each virus needed for infection has been optimized for maximal production of the two proteins ...
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bioRxiv - Cell Biology 2022Quote: All recombinant condensin complexes were expressed using insect cell strains from the Bac-to- Bac Baculovirus Expression System (ThermoFisher) as previously described (Kinoshita et al. ...
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bioRxiv - Plant Biology 2021Quote: The recombinant vectors were then transferred to Agrobacterium tumefaciens ElectroMAX™ LBA4404 cells by electroporation (Invitrogen, Carlsbad, CA, USA). The expression vector in A ...
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bioRxiv - Immunology 2020Quote: ... and 1µg/ml biotinylated recombinant hemagglutinin (rHA) (Whittle et al., 2014) conjugated to streptavidin APC (cat. no: S868, Invitrogen). To exclude dead cells ...
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bioRxiv - Cell Biology 2021Quote: ... reaction mixes were prepared using 2.5 μg recombinant His-ACBD5 with or without the addition of 0.3 mM ice-cold ATP (Thermo Fisher) and 0.1 ug GST-GSK3β (Abcam) ...
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bioRxiv - Microbiology 2020Quote: ... Generation of bacmid DNA and recombinant baculovirus was performed according to protocols from the Bac-to-Bac system (Invitrogen). All secreted proteins were purified from culture supernatant using streptactin beads (IBA ...
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bioRxiv - Molecular Biology 2022Quote: ... these plasmids were transformed into DH10MultiBac electrocompetent cells and recombinant baculoviruses produced according to manufacturer’s instructions (Bac-to-Bac expression system, Invitrogen). All constructs were verified by sequencing (VK8 or VK10/WA2 ...
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bioRxiv - Cell Biology 2022Quote: All recombinant Cdh23 variants for in-vitro studies were expressed in the ExpiCHO suspension cell system (A29129 ThermoFisher Scientific), following the prescribed protocol for transfection in ExpiCHO cells ...
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bioRxiv - Biophysics 2019Quote: ... Cells were lysed by sonication and recombinant proteins purified by Ni2+ affinity chromatography in Ni-NTA agarose (Thermo Fisher) using 50 mM TrisHCl/500 mM NaCl/10 mM imidazole [ph 8.0] as binding buffer ...
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bioRxiv - Cancer Biology 2019Quote: ... The following additional niche factors were added to generate “Full” media: 50 ng/ml mouse recombinant EGF (Life Technologies), 100 ng/ml mouse recombinant Noggin (Peprotech) ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transfected with recombinant lentiCRISPRv2 together with psPAX2 and pMD2.G using Lipofectamine 2000 (Thermo Fisher Scientific). Undigested lentiCRISPRv2 plasmid lacking sgRNA sequence was used for pseudovirus production as a control ...
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bioRxiv - Immunology 2020Quote: Recombinant SARS-CoV-2 S-2P trimer was biotinylated using the EZ-Link Sulfo-NHS-LC Biotinylation Kit (ThermoFisher) and tetramerized with streptavidin-APC (Agilent ...
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bioRxiv - Cell Biology 2020Quote: ... the nucleotide sequence was inserted by means of a BP recombinant reaction (Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) according to the manufacturer’s protocol into a pDONR221 donor vector ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL human recombinant epidermal growth factor (Gibco, USA). Cells were maintained in a humidified incubator at 37°C with 5% CO2 level ...
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bioRxiv - Microbiology 2020Quote: ... at day 0 the animals were subcutaneously administered either approximately 10,000,000 fffus of detergent-inactivated UHV or 0.1 mg of recombinant UHV NP emulsified in Freund’s incomplete adjuvant (ThermoFisher Scientific), the total volume per individual was 125 μl ...
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bioRxiv - Microbiology 2021Quote: Cells were transfected with recombinant plasmid p3xFLAG-NS5A or NS5A mutant expression vectors using Lipo3000 (Thermo Fisher Scientific, USA) according to the manufacturer’s directions ...
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bioRxiv - Genomics 2021Quote: Total RNA was extracted using the hot-phenol method (Schmitt et al., 1990) and treated with recombinant DnaseI (Ambion). For real-time reverse transcription PCR ...
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bioRxiv - Genetics 2020Quote: ... the cells and medium were transferred to fresh vitronectin (VTN)-coated 6-well tissue culture plates (10μl truncated vitronectin recombinant human protein (Invitrogen) + 1ml PBS (5μg/ml VTN ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μL of capture Ab (~4 μg/ml) or SARS-CoV-2 recombinant antigen (~0.2 mg/ml) in 1x PBS (Gibco) was loaded in each channel ...
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bioRxiv - Immunology 2022Quote: ... recombinant GBPs were incubated with Alexa Fluor 488 C5 maleimide dye or Alexa Fluor 647 C2 maleimide dye (Invitrogen) in a ratio of 1:1 or 1:2 on ice for 10–20 min ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Neuroscience 2023Quote: Sf9 cells for generation of baculovirus and expression of recombinant antibody fragment are from Thermo Fisher (12659017, lot 421973). The cells were not authenticated experimentally for these studies ...
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bioRxiv - Physiology 2023Quote: A recombinant adenovirus expressing mouse Sult2b1 (Ad-Sult2b1) was produced using the ViraPower Adenoviral Expression System (Invitrogen, Carlsbad, CA), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Endpoint PCR for all mouse CASK experiments was performed using 0.25 U recombinant Taq DNA Polymerase (Thermo Fisher Scientific) with 2 mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were cultured on 6-well plates coated with vitronectin recombinant human protein (A14700, Gibco, concentration 0.5 µg/cm) and maintained in Essential 8 Flex medium (A2858501 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Batches of ten 8-10 weeks old female BALB/c mice were inoculated intraperitoneally (i.p) with 105 PFU of Addavax-adjuvant recombinant virus in 200 μL of phosphate-buffered saline (PBS; Gibco). At twenty-one days post immunization ...