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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for 6 Bromo 3 N ethylamino 1 2 4 triazolo 4 3 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... in a 1:3 ratio using lipofectamine (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... PBS containing 1 µM TO-PRO 3 (Thermo Scientific) was applied to each section followed by 3 washes with PBS ...
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bioRxiv - Cell Biology 2020Quote: ... with TO-PRO-3 (#T3605, ThermoFisher, 1:1000 dilution).
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bioRxiv - Neuroscience 2021Quote: ... 1-hexanol (99%, ACROS Organics, CAS 111-27-3), 2-oxovaleric acid (>98% ...
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bioRxiv - Microbiology 2021Quote: ... 3-cholamidopropyl dimethylammonio 1-propanesulfonate (CHAPS; zwitterionic; Thermo Scientific). In addition ...
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bioRxiv - Developmental Biology 2022Quote: IF blocking buffer: 1/3 Blocker Casein (ThermoFisher 37528), 2/3 HEPM with 0.05% Triton X-100
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bioRxiv - Molecular Biology 2021Quote: ... containing 1 M sodium chloride (S671-3, Fisher Scientific). The labelling protocol was carried out at pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: ... rabbit anti-Neuroligin-3 (1:1000, Thermo Fisher Scientific), rabbit anti-COX-2 (1:1000 ...
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bioRxiv - Developmental Biology 2023Quote: ... and TO-PRO-3 (1:10,000, Thermo Fisher Scientific). Images were taken using a 20x oil immersion and a 10x dry objective on a Leica TCS SP8 confocal microscope.
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bioRxiv - Developmental Biology 2023Quote: ... Immunofluorescence blocking solution: 1/3 Blocker Casein (ThermoFisher Scientific), 2/3 HEPM with 0.05% TX-100 ...
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bioRxiv - Cancer Biology 2023Quote: ... and benzonase (1:100, Fisher Scientific, 70-664-3) on ice for 30 minutes ...
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Biophysics 2019Quote: ... 2 mM EDTA (ACROS Organics, cat# AC118432500, CAS: 60-00-4), 40 mM tris ...
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bioRxiv - Neuroscience 2020Quote: Primary cortical neurons were loaded with 2 µM Fluo-4 (Invitrogen) in KRH (Krebs’–Ringer’s–HEPES containing (in mM) ...
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bioRxiv - Neuroscience 2020Quote: ... Cultures were loaded with 2 µM Fluo-4 AM (Thermo Fisher) in Neurobasal-A media supplemented with B27 without phenol red for 30 minutes at 37°C in a 5.5% CO2 atmosphere ...
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bioRxiv - Cell Biology 2019Quote: ... Then Fluo-4 AM (2 µM final concentration) (Thermo Fisher, F14201) was added directly to the medium and incubated for another 30 min at 37℃ ...
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bioRxiv - Cancer Biology 2019Quote: ... and 2 µM Fluo-4 AM (Thermo Fisher Scientific, Waltham, USA). After a loading time of 45 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were incubated with 2 μM Fluo-4 AM (Invitrogen) diluted in HBSS solution (0.4 gL−1 KCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 mM MgCl2 and 2 µl of Platinum Taq polymerase (Invitrogen) with added water to a final volume of 25 µl ...
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bioRxiv - Immunology 2020Quote: ... 25mM HEPES (2(−4-(2-hydroxyethyl)-1-piperanzinyl) ethansulfonacid) and supplemented with 10% (v/v) fetal bovine serum (FBS; Gibco, ThermoFisher Scientific), 10.000 U/mL (v/v ...
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bioRxiv - Cancer Biology 2023Quote: ... were mixed in a 3:1 with sample buffer (NuPAGE™ LDS Sample Buffer (4X) + 2-Mercaptoethanol in 2:1) and loaded onto a precast Gel (NuPAGE™ 4-12% Bis-Tris Gel, Invitrogen). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
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bioRxiv - Neuroscience 2019Quote: ... Fluo-3-acetoxymethylester (Fluo-3-AM) (Molecular Probes-Thermo Fisher Scientific) was used (Beauvais et al. ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2022Quote: ... 3-6 dpf larval zebrafish were anesthetized using 0.2 mg/mL Tricaine-S (Fisher Scientific, NC0872873) solution ...
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bioRxiv - Biochemistry 2021Quote: ... 3-[p-(6-phenyl)-1,3,5-hexatrienyl] phenylpropionic acid was purchased from Molecular Probes (Eugene, OR, USA) and 1-stearoyl-2-linoleoyl-sn-glycerol-3-phosphocholine (SLPC ...
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bioRxiv - Neuroscience 2023Quote: The brains of P9-11 C57 mice were immersed and fixed for 3 days in cold 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide HCl (EDC, Thermo Fisher Scientific, in 0.1 M PB, pH 7.4) at 4°C ...
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bioRxiv - Physiology 2020Quote: Caspase-3-like activity was quantified using the EnzChek Caspase-3 Assay kit #1 (Molecular Probes, Eugene, OR, USA). Tissue samples were thawed on ice for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Cell Biology 2022Quote: ... HCEnCs were rinsed in DPBS and passaged at a ratio of 1:2 or 1:3 with TrypLE (Thermo Fisher Scientific) for 10-15 min at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... The sporozoite-infected culture was maintained for 3 or 5 or 7 days after which the cells were fixed with 4% paraformaldehyde (ThermoFisher Scientific: catalogue number 28906) for 10 minutes ...
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bioRxiv - Immunology 2021Quote: 293T cells grown in 96-well plates (3-4×104 cells / well) were co-transfected by Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA USA) with reporter plasmids ...
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bioRxiv - Neuroscience 2021Quote: ... Neurons were transfected at DIV 3-4 with mRFP-Rab7 (0.8 μg DNA per well) using Lipofectamine 2000 (Thermo Fisher Scientific, Waltham, MA, USA) followed by fixation (4% PFA ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was centrifuged at 1000 g for 3 min at 4 °C to remove debris and then the supernatant was incubated with anti-HA magnetic beads (Thermo Fisher Scientific, cat. # 88837) for 6 minutes at 20 °C ...
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bioRxiv - Biochemistry 2023Quote: ... blotted from both sides for 3 seconds at 4 ℃ under 99% humidity and plunge frozen in liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, MA). Images were recorded using a Titan Krios G4 microscope at the University of Tokyo (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
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bioRxiv - Microbiology 2021Quote: ... 1,2-Bis(2-aminophenoxy)ethane-N,N,N’,N’-tetraacetic acid tetraacetoxymethyl ester (BAPTA-AM, Invitrogen), calcium Ionophore A23187 (Sigma) ...
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bioRxiv - Physiology 2020Quote: ... 6-diamidino-2-phenylindole (DAPI, 1:25000, Thermo Fisher) diluted in PBS and mounted with Prolong Gold Antifade Medium (Invitrogen) ...
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bioRxiv - Molecular Biology 2020Quote: ... linker oligos with a 5’-terminal amine modification were obtained from IDT (Coralville, Iowa) and activated using sulfo-SMCC (Sulfosuccinimidyl-4-(N-maleimidomethyl)cyclohexane-1-carboxylate) reagent (Thermo Fisher, #A39268) according to manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... and 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Gibco 15630080), and incubated at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... buffered with 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... stock solutions were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The thrombin-selective inhibitor PPACK.2HCl and MMP Inhibitor V (ONO-4817 ...
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bioRxiv - Cell Biology 2021Quote: ... total 2 μg was diluted into 6 μl of reagent (1:3 ratio) in the 100 μl of Opti-MEM I medium (31985062, Thermo Fisher Scientific) and incubated for 15 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were rinsed with PBS (3×10min) and incubated for 6 hours with secondary donkey anti-rabbit 488 antibodies (1:1000, ThermoFisher Scientific, A11006) and streptavidin 594 (1:750 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Biochemistry 2020Quote: ... Coverslips were mounted and cells were counterstained on the glass slides using ProLong Gold with DAPI (4’, 6-diamidino-2-phenylindole) (Molecular Probes, Eugene, OR, US). Images were collected using an Axio Plan2/AxioCam microscope and image processing was performed with MrC5/Axiovision software (Zeiss ...