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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for Somatostatin Receptor 1 SSTR1 Rabbit Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Alexa Fluor 488 labeled donkey anti-Rabbit IgG (2 μg/ml, Molecular Probes, Eugene, OR USA) and a Cy3 conjugated AffiniPure donkey anti-rat IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... and secondary goat anti-rabbit IgG fluorescently labeled with Alexa Fluor 488 (A-11008, ThermoFisher Scientific). Fluorescence images were acquired using a 60X oil immersion objective on the EVOS M5000 Imaging microscope.
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bioRxiv - Neuroscience 2021Quote: ... brain tissues were incubated in secondary antibody solution (Alexa488 donkey anti-mouse secondary antibody, 1:1,000; A21202, Life Technologies; RRID:AB_141607) at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... The following secondary antibodies from Thermo Fisher Scientific were incubated at room temperature for one hour: Donkey anti-rat Alexa488 (1:100, Thermo Fisher, Cat # A-21208), Donkey anti-rat Alexa594 (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... The NFAp were incubated with primary antibodies for 1 h followed by secondary antibodies conjugated with either Alexa488 or Alexa594 (Invitrogen). SlowFade Gold Antifade Mountant (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated with primary antibodies in 10% NGS/NDS in PBST over night at 4°C following 1h incubation at 24°C with appropriate Alexa488/568 coupled secondary antibodies (1:1000, Invitrogen). Cell nuclei were stained with DAPI and final images were acquired using Leica TCS SP8 confocal microscope (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... slices were washed 3 times in PBS and incubated for a further hour at room temperature in diluted blocking solution containing 1:500 goat anti-mouse Alexa488-conjugated IgG secondary antibody (#A28175, RRID:AB_2536161, Invitrogen, Carlsbad, CA) and 1:5000 Hoechst stain (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were washed with permeabilization/blocking solution and stained with a 1:400 dilution of Alexa488-conjugated anti-mouse antibody (Invitrogen) for 1h at 4°C ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibodies used were 1:5000 anti-rat Alexa488 and anti-mouse Alexa594 conjugated antibodies (Cat #A32740 Invitrogen, CA, USA). The sections were mounted using ProLong Gold with DAPI (Cat #S36964 Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were labeled overnight at 4 °C with Alexa Fluor 488-labeled wheatgerm agglutinin (1:200; W11261, Thermo Fisher) in combination with a rabbit polyclonal primary antiserum ...
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bioRxiv - Cell Biology 2021Quote: The SFI1 antibody was raised in rabbit against the GST-fused C-terminal domain of SFI1 (aa1021 to aa1240) (Figure S1) and affinity-purified on AminoLink® Coupling Resin (20381 Thermo Fisher) coupled to the MBP-fused C-terminal domain (using the same aa sequence).
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bioRxiv - Evolutionary Biology 2020Quote: ... Hexahistidine-tagged chitinase proteins were purified from the concentrated culture filtrates or cellular lysates by metal affinity chromatography (HisPur Co2+ Resin, Thermo Fisher Scientific) and the chitinase-containing elution fractions exchanged into PBS by ultrafiltration ...
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bioRxiv - Immunology 2021Quote: ... Recombinant S and RBD trimeric proteins secreted in cell culture supernatants were purified using the CaptureSelect™ C-tag Affinity Matrix (ThermoFisher Scientific). Concentration of purified recombinant Abs were determined by PierceTM BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Biotinylated constituents in 600 μg of cell protein lysate were affinity purified by incubation with 30 μL of NeutrAvidin conjugated Sepharose beads (Thermo Fisher Scientific) for 2 h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Five days following transfection cellular supernatants were harvested and SIgA complexes were purified using CaptureSelect™ LC-lambda (Mouse) Affinity Matrix (Thermo Fisher) and Superose 6 (GE Healthcare Life Sciences ...
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bioRxiv - Immunology 2020Quote: ... The C-tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 5 mL CaptureSelect™ C-tag Affinity Matrix (ThermoFisher Scientific). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Immunology 2021Quote: ... BirA was expressed recombinantly in Escherichia coli as a genetic fusion to thioredoxin and purified with HisPur Ni-NTA affinity resin (Thermo Fisher Scientific). BirA-thioredoxin was subsequently loaded onto a GE Superdex S200 increase 10/300 GL column pre-equilibrated in 1X PBS on a GE ÄKTA Pure system before being aliquoted and frozen with 10% glycerol.
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bioRxiv - Immunology 2022Quote: ... VRC07 IgG antibodies were then purified from the culture supernatant using affinity chromatography with Pierce Protein A/G Agarose (Thermo Fisher Scientific) followed by size-exclusion chromatography using a Superdex 200 column (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Fc-fused antibodies and full-length antibodies and their respective complexes with cSC were purified using Capture Select IgA affinity resin (Thermo Scientific: 194288010) and eluted using 0.1M glycine at pH=3 ...
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bioRxiv - Cell Biology 2022Quote: ... An antibody against GFP (Millipore, ab16901, and Alexa488-anti-chicken, Thermo Scientific) was used to enhance the GFP signal if needed ...
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bioRxiv - Biophysics 2019Quote: ... was acquired from Lumiprobe and Alexa Fluor® 488 C5-Maleimide (Alexa488) was acquired from ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... followed by Alexa488 conjugated goat anti-mouse secondary antibodies (Invitrogen A-11001) diluted at 1:100 and 1:500 ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with anti-goat Alexa488 (A-11055, Thermo Fisher Scientific) and anti-mouse Alexa555 (A21427 ...
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bioRxiv - Molecular Biology 2020Quote: ... Following three PBST washes a secondary goat antimouse Alexa488 antibody (Molecular Probes) was incubated for 30 minutes at room temperature before PBST washes ...
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bioRxiv - Biochemistry 2022Quote: ... and Alexa488-Goat-anti-Mouse IgG (H+L) (Life Technologies A-11029). After washing and resuspension in FACS buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies used for immunofluorescence were goat anti-mouse-Alexa488 (ThermoFisher, A11029), goat anti-mouse-Alexa568 (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies included donkey-anti-goat-Alexa488/568 (Life Technologies A3214; A3214), goat-anti-rabbit-Alexa488/568 (Life Technologies A32731 ...
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bioRxiv - Immunology 2022Quote: ... CaptureSelect™ Alexa488 anti-C-tag conjugate (Thermo Fisher Scientific, Cat#7213252100), MLKL polyclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies included donkey-anti-goat-Alexa488/568 (Life Technologies A3214; A3214), goat-anti-rabbit-Alexa488/568 (Life Technologies A32731 ...
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bioRxiv - Bioengineering 2023Quote: ... The secondary antibody conjugated to Alexa555 or Alexa488 were purchased from Invitrogen, Thermofisher Scientific (France).
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bioRxiv - Microbiology 2024Quote: ... cells were stained with Alexa488-conjugated anti-mouse secondary antibody (Thermo Fisher) for 1 h at 37°C and then with DAPI (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2022Quote: ... Bound Q-GRFT was detected using an HRP-conjugated goat anti-rabbit polyclonal antibody at 1:5000 dilution and TMB (Cat. #65-6120 and #N301, respectively, Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Systems Biology 2019Quote: ... the membrane was probed overnight at 4°C with the Rabbit anti-TAP polyclonal primary antibody (Thermo Fisher, 1:5000 dilution in blocking solution), which recognizes an epitope at the region between the TEV-cleavage site and His6 ...
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bioRxiv - Biophysics 2019Quote: ... Cells labeled for Syt-7 were then incubated for 60 minutes with polyclonal cross-adsorbed un-conjugated F(ab’)2-Goat anti-Rabbit IgG (Invitrogen, ThermoFisher, Cat. # A24539, 1:300) diluted in TBS-0.2% BSA solution to bind the possibly remaining free primary antibody sites (anti-Syt-7 ...
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bioRxiv - Microbiology 2021Quote: ... sc-376764) and anti-PbGAPDH rabbit polyclonal serum (1:100 dilution) or anti-mouse CD3 rat monoclonal antibody (Invitrogen, 14-0032-82; 1;100 dilution) for 16 h at 4°C ...
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bioRxiv - Immunology 2024Quote: ... sections were blocked for endogenous peroxidases and non-specific binding before applying primary antibodies rabbit polyclonal anti-Gas6 (ThermoFisher, # PA5-79300, dilution 1:100) and rabbit polyclonal anti-Spp1 (ThermoFisher ...
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bioRxiv - Molecular Biology 2023Quote: ... briefly fixed with 4% PFA in PBS and probed again with the other rabbit primary antibody directly labeled using either the Zenon rabbit IgG labeling kits (Invitrogen, Z25302 or Z25306), or the Mix-n-Stain CF647 Antibody labeling kit (Sigma ...
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bioRxiv - Genomics 2020Quote: ... The membranes were incubated with diluted primary rabbit polyclonal ptprb (VE-PTP)(PA5-68309, Invitrogen, USA) (1:1000) ...
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bioRxiv - Microbiology 2020Quote: ... The membranes were incubated with rabbit anti-SARS coronavirus spike protein polyclonal antibody (Invitrogen, PA1-41513) diluted 1:10,000 ...
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bioRxiv - Immunology 2020Quote: ... 50μL of polyclonal anti-human β2M HRP-conjugated IgG detection antibody raised in rabbits (ThermoFisher Scientific), diluted 1:2500 in 2% BSA (0.2μg/mL) ...
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bioRxiv - Cell Biology 2021Quote: ... the following peptide was used to generate polyclonal antibodies in rabbits: ARLDH(AcK)FDLMYAK (ThermoFisher Scientific). The antibodies were purified via negative selection against non-acetylated peptide ARLDHKFDLMYAK (ThermoFisher Scientific).
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bioRxiv - Developmental Biology 2022Quote: ... saline tween (TBST) and then probed with a rabbit polyclonal antibody (Thermo Fisher Scientific, Waltham, MA) at 1:100 in blocking buffer overnight at 4C ...
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bioRxiv - Microbiology 2021Quote: For the reciprocal Co-IP using CHMP4B antibody (Thermo Fisher, rabbit polyclonal, cat no. PA5-64271), 7.5µg of antibody was conjugated to Dynabeads per manufacturer guidelines ...
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bioRxiv - Physiology 2021Quote: ... Samples were then incubated in secondary antibody (goat polyclonal antibody to rabbit IgG, A11008, Life Technologies) 1:200 in blocking solution ...
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Cellular identity and Ca2+ signaling activity of the non-reproductive GnRH system in the Ciona larvabioRxiv - Neuroscience 2020Quote: ... rabbit anti-green fluorescent protein (GFP) polyclonal (A11122; Thermo Fisher Scientific; for G-CaMP8 and CFP), rat anti-red fluorescent protein (RFP ...
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bioRxiv - Physiology 2020Quote: ... Blocks were sectioned at 16 um at stained using rabbit-anti GFP Polyclonal Antibody (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... or polyclonal goat anti-Rabbit Alexa Fluor546 to detect SP140 protein (Orange color) (A-11035, Invitrogen). Slides were mounted in SlowfadeGold reagent containing DAPI (4′,6-diamidino-2-phenylindole ...
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bioRxiv - Immunology 2020Quote: ... Cells were washed and then incubated with polyclonal anti-rabbit IgG (H+L) (Thermo Fisher Scientific) for 1 hour at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... tissue sections were incubated with the primary antibody (rabbit polyclonal anti-Influenza A virus NP [ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was diluted 1:1 with cold H2O and loaded onto a C-tag Affinity Column (ThermoFisher) equilibrated with C-tag Binding Buffer ...