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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Somatostatin Receptor 1 SSTR1 Rabbit Polyclonal affinity purified Alexa488 labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... THP-1 cells were labeled with calcein-AM (Invitrogen) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: ... THP-1 labeled with CellTracker Deep Red (ThermoFisher, C34565) were resuspended in HBSS supplemented with 1 mM Ca2+ ...
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bioRxiv - Plant Biology 2019Quote: For the co-immunoprecipitation (CoIP) 8 μl affinity-purified anti-CP31A antibody (Kupsch et al., 2012) was bound to 50 μl Dynabeads ProteinG (Invitrogen) under rotation (15 rpm) ...
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bioRxiv - Immunology 2019Quote: ... The purified mAbs were immediately passed over a custom made rhTNFα affinity column (NHS-activated agarose beads, Thermo Fisher Scientific) in gravity mode ...
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bioRxiv - Immunology 2022Quote: ... CCL2 was affinity purified from the in vitro reactions using Phytip streptavidin coated tips (Phynexus) bound to biotinylated anti-CCL2 antibody (Invitrogen) using the Phytip protocol ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Lysate was cleared by centrifugation and the protein was purified by nickel affinity chromatography (Thermo Scientific HisPur Ni-NTA Resin). After applying cleared lysate to the resin on-column ...
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bioRxiv - Biophysics 2020Quote: ... The proteins in the periplasmic extract were released by osmotic shock and purified by immobilized metal affinity chromatography (IMAC) using Ni-NTA resin (Invitrogen) in TBS buffer (20 mM Tris ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody was affinity purified on phospho-peptide covalently bound to a SulfoLink column according to the manufacturer’s instructions (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... The RM19R IgG was produced by transient transfection of Expi293 cells and purified using Protein A affinity chromatograph by Invitrogen. The RM19R Fab heavy chain plasmid was made introducing two stop codons following residue D234 in the RM19R IgG1 heavy chain vector using the QuikChange® Lightning Site-Directed Mutagenesis kit (Agilent) ...
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bioRxiv - Microbiology 2020Quote: ... The His-tagged protein was purified from the cleared cell lysate by nickel affinity chromatography (Ni-NTA agarose beads, Invitrogen). After washing with increasing concentrations of imidazole ...
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bioRxiv - Immunology 2021Quote: ... 2-43 and H4 by digestion with Papain in the presence of the reducing agent 30 mM cysteine and were purified by affinity chromatography on protein A following the manufacturer’s protocols (Thermo fisher). The purity of the resultant Fabs was analyzed by SDS-PAGE ...
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bioRxiv - Biochemistry 2022Quote: ... The proteins were first purified by affinity chromatography on a Ni-nitrilotriacetic acid (Ni-NTA) agarose resin (Thermo fisher scientific). The column was washed with buffer A supplemented with 20 mM imidazole ...
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bioRxiv - Immunology 2022Quote: ... Antibodies were purified on protein G affinity purification column and purity was assessed by SDS-PAGE followed by SafeStain blue staining (ThermoFisher). All antibody preparations were >95% pure.
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bioRxiv - Synthetic Biology 2022Quote: Fluorescent proteins were purified in batch using His-tag affinity chromatography at room temperature according to the resin manufacturer’s instructions (Thermo Fisher). Lysates were supplemented with 10 mM imidazole and 600 μl was applied to HisPur Cobalt resin (300 μl ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was isolated by centrifugation for 30 min at 4°C at 18,500 × g and purified by glutathione affinity chromatography (Thermo Fisher) on a gravity column ...
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bioRxiv - Biochemistry 2022Quote: ... proteins were purified via immobilized-metal ion affinity chromatography (IMAC) using a HisPur™ Cobalt Spin Plate (Thermo Fisher Scientific) equilibrated with equilibration buffer (50 mM Na2HPO4/NaH2PO4 ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µg of affinity-purified antibodies were coupled to 15 µL of pre-equilibrated Protein G Dynabeads (30 µL bead suspension, Invitrogen) for one reaction ...
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bioRxiv - Immunology 2023Quote: Affinity purified SARS-CoV-2 (Wuhan) RBD was biotinylated using EZ-Link Sulfo-NHS-LC-biotin (Life Technologies, USA, A39257) accordingly to manufacturing protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and NbE contained in periplasmic lysate purified by Ni2+-affinity (HisPur Ni NTA Resin; Thermo Fisher Scientific, Catalog number 88221) and eluted by low pH by adding Acetate Buffer (50 mM NaAc,1M NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... The fusion proteins were first purified in batch by a Ni2+-affinity step (HisPur Ni-NTA Resin; Thermo Fisher Scientific), before being further purified by size-exclusion chromatography (Superdex 75 Increase 10/300 GL) ...
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bioRxiv - Molecular Biology 2024Quote: ... nuclear lysates of inputs and affinity-purified elution were loaded on a 4-20% Tris-glycine gel (Invitrogen, Cat# XP04205BOX), 10% acetic acid and 50% methanol was used to fix the gel in for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Alexa488 anti-rat and Cy3 anti-rat (1:250, Jackson) secondary antibodies or with Alexa647-conjugated Streptavidin (1:200, Thermo Fisher Scientific) and by counterstaining with DAPI (0.2 µg/ml ...
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bioRxiv - Cell Biology 2019Quote: We used the following mouse monoclonal antibodies against: transferin receptor (13-6800, Invitrogen, 1/200); CD63 [53] ...
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bioRxiv - Neuroscience 2020Quote: ... IgG1 anti-transferrin receptor (TfR, clone H68.4, 1:500, ThermoFisher Scientific, catalog no. 13-6800), IgG1 anti-GAPDH (1:10,000 ...
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bioRxiv - Physiology 2023Quote: ... The anti-transferrin receptor mouse monoclonal antibody (TransR, clone H68.4, Thermo Fisher Scientific, 1:1000) and the anti-alpha 1 Na+/K+-ATPase mouse monoclonal antibody (Na+/K+-ATPase α1 ...
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bioRxiv - Biophysics 2022Quote: ... The blot was then incubated for 10 min at room temperature with a 1:1000 dilution of rabbit polyclonal anti-ASIC1a antibody (OSR00097W, Thermo Fisher Scientific) in blocking buffer followed by 10 min incubation at room temperature with goat anti-rabbit IgG/horseradish peroxidase-conjugate (1:10,000 dilution of 1 mg/mL stock in blocking buffer ...
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bioRxiv - Epidemiology 2019Quote: ... for 2 h at room temperature and incubated overnight at 4°C with rabbit polyclonal anti-myeloperoxidase (MPO, 1:100, Invitrogen, CA, USA) or rabbit monoclonal anti-F4/80 (1:100 ...
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Cell culture dimensionality influences mesenchymal stem cell fate through cadherin-2 and cadherin-11bioRxiv - Cell Biology 2019Quote: ... Primary antibodies used were rabbit polyclonal anti-cadherin-11 and mouse monoclonal anti-cadherin-2 (3B9) (both 1:100; Thermo Fisher Scientific). Secondary antibodies used were goat anti-mouse Alexa Fluor 488 and goat anti-rabbit Alexa Fluor 647 (both 1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies [Anti GFP rabbit polyclonal antibody (1:500 dilution; #catalog no: A-11122, Thermo Fisher Scientific, Tokyo, Japan)] diluted with the blocking solution overnight at 4°C ...
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bioRxiv - Genomics 2021Quote: ... nuclei were incubated for 1 hour in a solution containing 5% FBS and 1:500 primary antibody (H3K9me3; Invitrogen rabbit polyclonal 49-1008 or H3K9Ac; Invitrogen 49-1009) in 1x PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were again rinsed in PBS and incubated in carrier solution with AlexaFluor-568 secondary anti-rabbit polyclonal goat antibody (1:1000; Thermo Fisher Scientific) and Streptavidin AlexaFluor-488 (1:750 ...
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bioRxiv - Neuroscience 2021Quote: ... The specimens were blocked and permeabilized using permeabilization buffer for 2 h and incubated with rabbit polyclonal antibody anti-eNOS (1:200; PA1-037; Thermo Fisher Scientific) and goat polyclonal antibody anti–collagen IV (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4 °C with the primary antibody rabbit anti-CaMKIIα polyclonal antibody (1:1000, Invitrogen PA5-84083), mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Microbiology 2022Quote: ... 3D cultures were incubated at 4 °C overnight with primary antibodies in 1% bovine serum albumin (BSA)/PBS as follows: rabbit anti-uroplakin-1A (UPK1A) polyclonal antibody (1:100 dilution, PA5-49668, Thermo Fisher Scientific), rabbit anti-uroplakin-II (UPKII ...
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bioRxiv - Biochemistry 2022Quote: ... the membrane was incubated overnight with SARS/SARS-CoV-2 Coronavirus Spike Protein (subunit 1) rabbit polyclonal antibody (Thermo Fisher scientific, France) at a dilution of 1:1000 in the same buffer as for saturation ...
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bioRxiv - Molecular Biology 2023Quote: The following primary antibodies were used for the immunological detection of specific proteins on PVDF membranes: anti-Tap (CAB1001 rabbit polyclonal antibody, Invitrogen, 1:10.000), anti-HA (12CA5 mouse monoclonal antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 hours at room temperature before overnight incubation with rabbit polyclonal anti-ELAPOR1 antibody (Thermo Fisher Scientific, PA5-72691, 1:150). Cells were then washed three times with PBS for 15 min and incubated with anti-rabbit IgG (H+L ...
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bioRxiv - Neuroscience 2024Quote: ... Detection was performed with appropriate secondary antibodies: polyclonal goat anti-rabbit coupled to Alexa Fluor 488 (1:1000, Thermo Fisher, A-11008), polyclonal goat anti-rabbit coupled to Alexa Fluor 568 (1:1000 ...
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bioRxiv - Zoology 2021Quote: ... sections were incubated successively in PBST with 1) an anti-phosphorylated ribosomal protein S6 (pS6) rabbit polyclonal antibody (Ser244, Ser247 anti-pS6, 44-923G, Thermo Fisher Scientific, MA, USA; 1:5000) that was used in mouse OSNs as validated in Sharma et al ...
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bioRxiv - Neuroscience 2019Quote: ... followed by affinity purification using the peptide as the affinity ligand (Thermo Fisher Scientific). Anti-PCNA antibody (Santa Cruz sc-56 ...
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bioRxiv - Cell Biology 2020Quote: Purified AHs were covalently labeled via cysteines using Alexa C5 maleimide probes (either 488 or 568; Thermofisher). Plin4 12mer ...
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bioRxiv - Bioengineering 2020Quote: Purified tmSCF protein was first labeled with Alexa Fluor™ 488 Microscale Protein Labeling Kit (Thermo Scientific), then tmSCFPLs and tmSCFNDs were fabricated using this labeled protein ...
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bioRxiv - Developmental Biology 2020Quote: Primary prenatal human microglia were MACS-purified as described above and labeled with DiI (Thermo Fisher, V22885) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: An ~800-bp purified P2A-mCherry DNA fragment was labeled with a red-dUTP (AF594, Molecular Probes) by nick translation ...
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bioRxiv - Microbiology 2021Quote: ... Anion exchange purified HMPV P was labeled in a similar manner using Alexa 594 NHS ester (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: ... 50 μM purified Tau was labeled with Alexa Fluor 594 dye following the manufacturer’s instruction (Thermo Fisher).
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bioRxiv - Bioengineering 2024Quote: ... The labeled IgG was purified using a Zeba Spin® desalting column 40K (Thermo Scientific, Waltham, MA). The purified labeled IgG was collected ...