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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 6 Methoxy 2 methyl 1H benz de isoquinoline 1 3 2H dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... diluted 1:2 with DPBS (Gibco), and centrifuged over Ficoll-Paque Plus (GE Healthcare) ...
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 2-3 hours at room temperature with 2 µg/ml donkey anti-goat Alexa Fluor 488 (Cat# A21206, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 568 (Cat# A10037 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1h at room temperature and mounted in ProLong Diamond Reagent with DAPI (Molecular Probes). Images were captured on a Zeiss LSM880 confocal microscope and analyzed with Fiji freeware.
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bioRxiv - Genomics 2019Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
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bioRxiv - Cancer Biology 2021Quote: ... imatinib or sunitinib for 1h or 8h were labelled with 25 µM HPG (Invitrogen, C10102) for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1h at room temperature and proteins visualized by chemiluminescence (Thermo Fisher Scientific). Each experiment was repeated at least four times.
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bioRxiv - Cell Biology 2020Quote: ... EDL muscles were dissected from tendon-to-tendon and incubated for 1h in DMEM (Gibco) containing 0.25% collagenase I (Worthington) ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 594-conjugated secondary antibodies was added for 1h at room temperature (Molecular Probes), and nuclei were counterstained using SlowFade Gold Antifade reagent (Vector ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were precleared 1h at room temperature with magnetic beads blocked with BSA (ThermoFisher Scientific) to avoid aspecific binding ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10 % (vol/vol) Fetal Bovine Serum (Gibco; Heat-inactivated for 1h at 70°C). Stable cell lines were obtained by transfection with pMT puro vectors containing either Zasp52-PF or Zasp52-PF-ABM using Effectene (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... during 1h at room temperature before mounting in ProLong Gold Antifade reagent (P36941, Life Technologies). F-Actin was stained using Actistain (1:150 ...
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bioRxiv - Cell Biology 2023Quote: ... for 1h at 37°C and then with Proteinase K (Fisher Scientific, #25-530-049) for 2h at 55°C ...
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bioRxiv - Genetics 2023Quote: ... transiently transfected HEK293T cells were starved for 1h in methionine-free RPMI (Thermo Fisher Scientific) and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer) ...
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bioRxiv - Genomics 2024Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then dipped in the secondary antibody-containing buffer with the nuclear dye 4′,6-diamidino-2-phenylindole (DAPI, Cat# D1306, Life Technologies-Invitrogen, Carlsbad, CA, USA, dilution 1:400), for 2 hours at RT ...
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bioRxiv - Microbiology 2020Quote: ... DNA quantity and quality were assessed in 1% agarose gel and using a Nanodrop 2000c spectrophotometer (Thermo Fisher Scientific Inc., Wilmington, DE, USA) set for determining absorbance at the following wavelengths ...
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bioRxiv - Physiology 2020Quote: All forward and reverse quantitative PCR (qPCR) primers (Table 1) were designed using Primer Express 3.0.1 (Applied Biosystems, ThermoFisher Scientific, Wilmington, DE, USA). Primers were designed using sequences from the brook trout transcriptome from Sutherland et al ...
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bioRxiv - Plant Biology 2024Quote: The degradation and contamination of RNA were monitored on 1% agarose gels and the quality and integrity of RNA were assessed using a NanoDrop spectrophotometer (840-317400, Thermo Scientific, DE, USA). A total amount of 1.5 μg RNA per sample was used as input material for the RNA sample preparations ...
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bioRxiv - Cell Biology 2020Quote: ... Adult cardiac NMCs were isolated from adult C56BL/6 mice (6-8 weeks old) by digesting adult ventricles in buffer containing 1 mg/ml collagenase IV (Gibco 17104-019) and 1.2 mg/ml dispase II (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... using a 10 min loading at 3 µL/min flow rate to a trap column (Acclaim™ PepMap™ 100, 2 cm × 75 µm, 3 µm, 100 Å - ThermoFisher Scientific). The separation was performed on an EASY-Spray™ C18 analytical column (25 cm × 75 µm ...
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bioRxiv - Neuroscience 2022Quote: ... NIM was exchanged daily for 3:1-DMEM/F12-medium (3 parts DMEM (Thermo Fisher Scientific, #31966047) and one-part F12 medium (Ham’s F-12 Nutrient Mix ...
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bioRxiv - Neuroscience 2019Quote: ... and monoclonal rat anti-mouse IL-6 (1:50; Invitrogen, MP5-20F3) were diluted in 3% normal donkey serum and incubated overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti phospho-tau PHF-6 (Thr231) (1:1000, Thermofisher #35-5200); mouse anti-β-Actin (1:20000 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged 1:6 once at 70% confluency using Versene (Gibco). Monocyte factories were set up following a previously reported protocol (van Wilgenburg et al. ...
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bioRxiv - Neuroscience 2023Quote: ... diluted 1:6 in DMEM/F12 media (11320033, Thermo Fisher, Waltham, USA). The iPSCs were maintained ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were passaged 1:6 once at 70% confluency using Versene (Gibco). Monocyte factories were set up following a previously reported protocol (van Wilgenburg et al. ...
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bioRxiv - Cell Biology 2023Quote: ... The remaining material was resuspended in 6 mL sterile 1× PBS (Gibco). Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated for 2–3 min at RT in trypsin (0.05% v/v; Gibco 25300-054), and tapped to release ...
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bioRxiv - Cancer Biology 2020Quote: ... and 12 mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494). After 4 hours ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μg of RNA was digested with Turbo DNase (2 U/μl, # AM1907, ThermoFisher Scientific). 500 ng of DNase-digested RNA was tagged with G/I tails in a 10 μl reaction ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated for 3 days in presence of 100 U/mL IL-2 (Gibco) and 1 µg/mL PHA (Remel).
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bioRxiv - Microbiology 2020Quote: ... The modified nucleotide 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated by PCR using DreamTaq™ DNA Polymerase (Thermo Scientific™) ...
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bioRxiv - Microbiology 2021Quote: ... or with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) for 30 min at room temperature (1:150 in AnnexinV binding buffer-Biolegend) ...
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bioRxiv - Microbiology 2021Quote: ... and 2-3 cm) after measuring the DNA concentration with Qubit (QubitⓇ 2.0 Fluorometer, Invitrogen). We obtained eight metagenomes from Lake Sarnen (deepest and shallow_inflow station for March ...
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bioRxiv - Evolutionary Biology 2022Quote: ... where an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles; Thermo Fisher) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Molecular Biology 2019Quote: ... an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles, Thermo Fisher Scientific) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2019Quote: ... Primer 2: 5’-GGATGTTCGTCCAGTGAGATTAG-3’) using a 7500 Fast Real-Time PCR System (Applied Biosystems) and accompanying software to analyze qPCR data.
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bioRxiv - Microbiology 2021Quote: ... an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)) assay (M6494, Thermo Fisher Scientific) was conducted in parallel to the infection and kinase inhibitor treatment according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Biochemistry 2021Quote: ... a pEntryslot3 vector containing the let-858 or tbb-2 3’UTR and pDEST (ThermoFisher) in an LR reaction ...
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bioRxiv - Biophysics 2019Quote: ... Medium was changed every 2-3 days and cells were harvested with TrypLETM Express (Invitrogen) at 80%-90% confluency ...
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bioRxiv - Cell Biology 2021Quote: siRNA transfections were performed 2 or 3 days before each experiment using Lipofectamine RNAiMax (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines were passaged every 2-3 days using 0.05% Trypsin/EDTA solution (Gibco).
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Genomics 2022Quote: ... The remaining RNA (< 3 µg) was treated with 2 U TURBO™ DNase (Invitrogen, USA) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Samples were DNase treated (3 µL of Turbo RNase-free DNase, 2 U/µL, ThermoFisher) for 20 min at 37°C and then precipitated by addition of 290 µL of water ...
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bioRxiv - Molecular Biology 2024Quote: ... passage 2-3 NPCs were detached with Stempro Accutase cell dissociation reagent (A1110501, Life Technologies) and kept in cold PBS 1%FBS for the duration of the protocol ...
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bioRxiv - Biochemistry 2024Quote: ... Cytotoxicity was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (ThermoFisher: M6494) reduction analysis according to manufacturer’s instructions ...