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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 6 Methoxy 2 methyl 1H benz de isoquinoline 1 3 2H dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 250 ng/ml 4′,6-diamidino-2′-phenylindole dihydrochloride (DAPI) or 500 nM SYTOX Orange (Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... After another set of gentle washes and 4′,6-diamidino-2-phenylindole (DAPI) nuclear staining (Thermo Scientific, R37606 ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei were visualized with 4′,6-diamidino-2-phenylindole (DAPI) in SlowFade Diamond antifade mounting medium (ThermoFisher). Primary antibodies were incubated with tissues overnight at 4°C in 1% normal donkey serum ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei staining was performed with 4’,6 diamidino-2-phenylindole dihydrochloride (DAPI, Molecular Probes, Eugene, OR, USA) at 300 nM in PBS ...
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bioRxiv - Genomics 2024Quote: ... Sections were stained with DAPI (4 ',6' -diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Imaging was performed on a Nikon Eclipse Ti Confocal at 20X objective and processed using Nikon Elements-AR ...
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bioRxiv - Neuroscience 2024Quote: HeLa cells (ATCC, #CCL-2) were plated in DMEM (Cellgro, #10-013CV) containing 6% FBS (Gibco, #26140) at 10,000 cells per well in 96-well tissue culture plates ...
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bioRxiv - Microbiology 2024Quote: ... hMDM nuclei were stained with 50 ng/ml 4’,6-diamidino-2- phenylindole (DAPI) (Invitrogen Cat. # D1306) for 7 min at room temperature ...
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bioRxiv - Genomics 2019Quote: ... RNA quantification was determined by NanoDrop 2000c Spectrophotometer (Thermo Fisher Scientific, Wilmington, DE).
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bioRxiv - Molecular Biology 2019Quote: ... TLPs were de-salted with a 5 ml Zeba spin column (Thermo Fisher) into 20 mM Tris pH 8.0 ...
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bioRxiv - Bioengineering 2019Quote: ... and a NanoDrop 1000 UV-vis spectrophotometer (NanoDrop Technologies/Thermo Scientific, Wilmington, DE). mRNA was isolated using an NEBNext Poly(A ...
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bioRxiv - Neuroscience 2020Quote: ... Isolated RNA was quantified using a NanoDrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA). 100 microgram of RNA was reverse transcribed using the iScript cDNA Synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... DNA concentration was then measured via Nanodrop Lite (Thermo Scientific, Wilmington, DE, USA) and subsequently diluted to 25 μg/μl ...
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bioRxiv - Plant Biology 2021Quote: ... DNA concentrations were measured using a Nanodrop spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and standardized to a concentration of 80 ng/μl.
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bioRxiv - Plant Biology 2022Quote: ... Samples were then de-paraffinized using Histo-Clear (Fisher Scientific, 50-899-90147) and re-hydrated by going through an ethanol series of 100 ...
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bioRxiv - Evolutionary Biology 2019Quote: ... DNA purity was checked using a NanoDrop ND2000 spectrophotometer (Thermo scientific, Wilmington, DE) and size distribution and degradation were assessed using a Fragment analyzer (AATI ...
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bioRxiv - Cell Biology 2021Quote: ... the actin de-polymerizer latrunculin B (10 μM; Tocris Bioscience; Thermo Fisher Scientific), or the YAP inhibitor verteporfin (0.5 μM ...
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bioRxiv - Microbiology 2020Quote: ... Aliquots were quantified using a Nanodrop 2000 (Thermo Fisher Scientific Inc.; Wilmington, DE). Whole genome shotgun libraries were prepared using the NEB Ultra Library Prep kit (New England Biolabs ...
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bioRxiv - Microbiology 2020Quote: ... were digested with BamH I and Xho I (NEB) (Thermo Scientific, DE, USA) and ligated together with T4 DNA ligase (Thermo Scientific) ...
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bioRxiv - Immunology 2020Quote: ... The protein was de-salted using Zeba Quick Spin columns (Thermo Fisher, 89849) and incubated with Streptavidin-AlexaFluor-647 (SA-AF-647 ...
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bioRxiv - Cell Biology 2021Quote: Paraffin sections of formaldehyde-fixed pancreas were de-paraffinized with Citrisolv (Fisher Scientific), re-hydrated in a decreasing graded series of ethanol ...
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bioRxiv - Genomics 2021Quote: ... For endoderm differentiation we used a PSC Definitive Endoderm (DE) Induction Kit (Gibco, ThermoFisher Scientific Cat ...
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bioRxiv - Microbiology 2024Quote: ... and quantified using a NanoDrop ND-2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). All RNA samples were pooled for sequencing using PacBio ISO-Seq and BGISEQ-500 Denovo polyA mRNA workflow (BGI Genomics Co. ...
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bioRxiv - Genomics 2023Quote: ... Aliquots were quantified using a Nanodrop 2000 (Thermo Fisher Scientific Inc.; Wilmington, DE). Genomic DNA extraction from clinical or mock specimens using the MagNA Pure platform (Roche Applied Science ...
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bioRxiv - Molecular Biology 2022Quote: ... De-salted peptides were dried using a SpeedVac vacuum centrifuge concentrator (Thermo Fisher) before TMT labelling.
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bioRxiv - Physiology 2023Quote: ... RNA purity/quantity was confirmed by Nanodrop spectrophotometry (Thermo Fisher Scientific, Wilmington, DE). Standard reverse transcriptase was performed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems by Life Technologies ...
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bioRxiv - Physiology 2023Quote: ... RNA quantity was measured with NanoDrop ND-1000 (Thermo Scientific, Wilmington, DE, USA) and quality was assessed with 2100 BioAnalyzer system (Agilent Technologies ...
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bioRxiv - Genetics 2023Quote: ... Following de-crosslinking of the ligated DNA concatemers with proteinase K (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was assessed with a Nanodrop Spectrophotometer (Thermo Scientific, Wilmington, DE, USA) and the concentration quantified using a broad-range assay in Qubit 3.0 Fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... DNA concentrations were determined using a Nanodrop-3300 fluorospectrometer (ThermoFisher, Nanodrop Wilmington, DE) with PicoGreen® reagent according to the manufacturer’s instructions (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was quantified using either Nanodrop (ND-1000, Thermo Fisher Scientific, Wilmington, DE) and/or a Qubit 2.0 Fluorometer (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... de-salted with a peptide cleanup C18 spin column (Thermo Scientific catalog #89870), then resuspended in 35 µL 0.1% formic acid (FA) ...
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bioRxiv - Cell Biology 2020Quote: ... 3 and 5 using Presto Blue (1:10 dilution in EM, 1h of incubation at 37°C, ex/em 560/590, Thermo Fisher Scientific, Waltham, MA). After 10 min of fixation using 4% neutral buffered formaldeyde (VWR ...
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bioRxiv - Neuroscience 2024Quote: ... samples were washed 3x 5min with PBST and counter stained for 1h with nuclear stain (Hoechst 33258) and secondary antibodies: rabbit antibodies - goat anti-rabbit conjugated with Alexa Fluor 647 (ThermoFisher, A27040, 1/1000 dilution); goat antibody - rabbit anti-goat conjugated with Alexa Fluor 647 (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... the following secondary antibodies were incubated at RT for 1h: anti-IgG rabbit (1:1000; Alexa Fluor 488 or 546, Life Technologies, A11008 or A11010), anti-IgG mouse (1:1000 dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... in a 2:2:1 molar ratio using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... and THPTA in ratio 1:2:2 in HBSS (Gibco #14025092). The later two components were also dissolved in water and a stock solution of 10 mM concentration each was prepared ...
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bioRxiv - Microbiology 2020Quote: ... and 1 ug/ml of interleukin-2 (IL-2; Gibco BRL) in complete RPMI 1640 containing 2mM L-glutamine ...
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bioRxiv - Cancer Biology 2019Quote: ... in a 2:2:1 molar ratio using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... 1% N-2-hydroxyethylpiperazine-N’-2-ethanesulfonic acid (HEPES, Gibco, #15630080) and 1% MEM nonessential amino acids (MEM-NEAA ...
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bioRxiv - Cancer Biology 2023Quote: ... in a 2:2:1 molar ratio using lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... or a 1:1 mixture of two siRNA to CHMP2A (5’-CAGGCCGAGAUCAUGGACAUG-3’ and 5’-GAAGAUGAAGAGGAGAGUGAC-3’) using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... were transfected 2h after shake off with 300 nM of siRNA oligonucleotides and Oligofectamine (Thermo Fisher Scientific; 12252011). Cells were fixed for immunofluorescence ...
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bioRxiv - Cell Biology 2021Quote: ... DSS fixation was performed for 2h at room temperature following slightly modified manufacturer’s protocol (Thermo Fisher Scientific MAN0011240). The FA fixed cells were washed with PBS and replaced with 0.5mM DSS dissolved in DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... Template plasmid was digested with DpnI for 2h at 37°C before amplification in Top10 competent cells (Invitrogen). Presence of S391A mutation was confirmed by sequencing.
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bioRxiv - Microbiology 2023Quote: ... The harvested lipid-rich material went through 2h dialysis within 2K molecular-weight cut off cassettes (Thermo Scientific) to be reconstituted into phosphate-buffered saline (PBS) ...
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bioRxiv - Plant Biology 2020Quote: ... the samples were centrifuged before receiving 50 µL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1% trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Systems Biology 2021Quote: ... the samples were centrifuged before receiving 50 μL of N-Methyl-M (trimethylsilyl) trifluoroacetamide (MSTFA) + 1 % trimethylchlorosilane (TMCS) (ThermoFisher Scientific, Waltham, MA, USA), briefly vortexed and incubated at 60 °C for 40 min ...
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bioRxiv - Synthetic Biology 2020Quote: ... Dried samples were dissolved in 250 µl of silylation-grade acetonitrile followed by addition of 250 µl of N-methyl-N- (trimethylsilyl)trifluoroacetamide (MSTFA) with 1% trimethylchlorosilane (TMCS) (Thermo Scientific, Bellefonte, PA) and heated for 1 hr at 70 °C to generate trimethylsilyl derivatives ...
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bioRxiv - Molecular Biology 2021Quote: ... cryosectioned zebrafish larvae were washed 3x in wash buffer for 5 min and subsequently stained with Filipin solution for 60 min in a humidified dark chamber and co-stained with BODIPY TR methyl ester (1:300, Life Technologies, Darmstadt, Germany). Staining solution was removed and slides were washed twice in wash buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... Firstly proteins were precipitated with 50 μL of acetonitrile followed by steroid extraction via liquid-liquid extraction with 1 mL tert-methyl butyl ether (MTBE, Acros Organics, Fisher Scientific, UK). The MTBE layer was then removed ...