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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 6 Chloro 2 3 diphenylimidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2019Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/µL) ...
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bioRxiv - Developmental Biology 2021Quote: ... After 30 min incubation with 100 ng/mL 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific D1306), specimens were washed three times with PBS−/− ...
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bioRxiv - Neuroscience 2021Quote: ... Isolated MEFs were plated in 2 wells of a 6-well plate and cultured overnight in DMEM (Gibco) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2021Quote: ... Cells fixed in methanol were incubated with 4’,6-Diamidino-2-pheny-lindoldihydrochloride (DAPI; Thermo Fisher Scientific #D1306) for 10 minutes and subsequently imaged at 6 locations per condition using a Leica DMi8 microscope ...
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bioRxiv - Cell Biology 2021Quote: ... Slides were mounted using ProLong™ Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific). Imaging was performed using a Leica TCS SP8 confocal microscope.
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bioRxiv - Neuroscience 2021Quote: ... incubated for 10 min in PBS/10 ug/mL 4’-6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to stain nuclei ...
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bioRxiv - Neuroscience 2022Quote: Immunofluorescence was performed using glial fibrillary acidic protein (GFAP) and 4′,6-diamidino-2-phenylindole (DAPI) solution (ThermoFisher). GFAP primary and secondaries were diluted to a ratio of 1:1000 and 5:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... Nuclei and F-actin were counterstained with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, 300 nM, D1306, ThermoFisher) and phalloidin-AlexaFluor 647 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... The slices were mounted with ProLong Diamond Antifade Mountant with 4’,6-diamino-2-phenylindole (DAPI) (ThermoFisher Scientific). Fluorescent images were analyzed with a Nikon confocal microscope (Nikon A1RMP ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... dishes were mounted with ProLong Diamond mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Waltham, MA), and imaged using a Zeiss LSM 780 laser confocal microscope equipped with a C-Apochromat 40x/1.2W objective.
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bioRxiv - Microbiology 2022Quote: C57BL/6 or db/db mice were ear-punched using a 2 mm Animal Ear-Punch (Fisher Scientific) and 1×108 CFU of bacteria topically applied to the entire mouse using sterile cotton swabs at Day 0 and Day 4 post ear-punch ...
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bioRxiv - Neuroscience 2022Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog #D3571), washed again ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected as follows: 2 μg eGFP-SHLD3 constructs and 6 μl Lipofectamine 2000 (Thermo Scientific) were incubated in Opti-MEM for 20 min at room temperature before being added to the cells whose media had been changed to McCoy’s with 10% FBS and no antibiotics ...
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
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bioRxiv - Zoology 2023Quote: ... The samples were stained by the 4’,6-diamidino-2-phenylindole (DAPI) and the rhodamine-conjugated phalloidin (ThermoFisher). The signals were detected by fluorescent microscopy (Nicon ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were washed 4 times with PBST and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) at 1:1000 dilution ...
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bioRxiv - Bioengineering 2023Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
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bioRxiv - Immunology 2023Quote: ... Slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted with ProLong Diamond Antifade Mountant (ThermoFisher). Images were acquired using a 20X objective lens Zeiss LSM 880 Confocal Laser Scanning Microscope.
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bioRxiv - Immunology 2023Quote: ... RD114 cells were seeded at 2 × 105 cells/well in a 6-well plate with DMEM (Gibco, USA) supplemented with 10% FCS (Sigma-Aldrich ...
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bioRxiv - Biophysics 2023Quote: ... Sample were rinsed in PBS and incubated with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific™, USA) for 5 mins and Alexa 594-phalloidin (Invitrogen™ ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
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bioRxiv - Cell Biology 2023Quote: ... HSPCs were incubated with 20µM NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen) for 30 minutes at 4°C for Golgi staining or Cytopainter (Abcam ...
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bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... ATs and TTs were fixed in 4% PFA and stained with 4′,6- diamidino-2-phenylindole (DAPI) (Invitrogen) to identify cell nuclei ...
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bioRxiv - Synthetic Biology 2024Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were washed five times in PBST and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D1306) and mounted in Aqua-polymount (Polysciences Ince. ...
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bioRxiv - Microbiology 2024Quote: ... Then, 4′,6-diamidino-2-phenylindole (DAPI, Solabio) and Alexa Fluor 488 Conjugate Concanavalin-A (Invitrogen, CA, USA) were added to each slide and incubated for 5 min at room temperature ...
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Neuroscience 2021Quote: ... the Ca+2 indicator Fluo-4-AM (Molecular Probes, Eugene, OR; 2–5 µl of 2 mM dye) were dropped over S1 cortex ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Cell Biology 2020Quote: ... in PBS and incubated for 2-3 hr in secondary antibodies (conjugated to Alexa-488, Alexa-568, and Alexa 647, Life Technologies,1:500) at room temperature ...
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bioRxiv - Developmental Biology 2020Quote: ... Rehydrated embryos were blocked with 10% BSA (in PBST) for 2-3 hours and incubated with anti-GFP antibody (1:1000; ThermoFisher Scientific, A-11120) overnight at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... HEK293T cells were seeded into 12-well plates and were transfected with 1.5 μg the RPL plasmid and 0.5 μg Cas13b gRNAs (NTC, U1-1, U1-2, U1-3) while ∼80% confluency using Lipofectamine 3000 (Thermo Fisher Scientific, Cat.# L3000015). For RIP experiments ...
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bioRxiv - Microbiology 2021Quote: ... The phospholipolytic activity was measured according to 83 using a synthetic fluorescent substrate 1-palmitoyl-2-(10-pyrenyldecanoyl)-sn-glycero-3-phosphocholine (Molecular Probes, The Netherlands) and a Hitachi F-4000 spectrofluorimeter.
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bioRxiv - Microbiology 2020Quote: ... Cells were infected in a BSL-3 lab with the UF-1 strain of SARS-CoV-2 at MOI of 4 in media containing 3% low IgG FBS (Fisher Scientific, Cat. SH30070.03).
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bioRxiv - Neuroscience 2019Quote: ... 3 PBS washes were performed and then 2 hours of incubation in CY-5 goat anti-rabbit (1:1000, Invitrogen, ThermoFisher Scientific, A10523) diluted in 2%NGS-PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were rinsed (3 times) with 2 ml incubation buffer and resuspended with anti-mouse Alexa Fluor 647 secondary antibody (1:2000, Invitrogen, Carlsbad, CA, USA) for 60 minutes at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were washed with cold 1× PBS-T for 3 times each for 10 min then incubated for 2 hours at RT with secondary antibodies (AF546-Ms: Invitrogen A11003, 1:500) diluted in 1× PBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... in 1X PBS-0.3% Triton X-100 (0.3% PBST) before 2-3 day incubation with primary antibodies at 4C for PCP4 (1:250, Invitrogen Cat# PA5-52209, RRID:AB_2645298) or NECAB2 (1:250 ...
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bioRxiv - Developmental Biology 2024Quote: ... HaCaT cell transfection with a 75 pM siRNA mix (#2316-1, #2316-2, and #2316-3) was conducted using Lipofectamine RNAiMAX transfection reagent (Invitrogen, Carlsbad, CA, USA), following the manufacturer’s guidelines.
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bioRxiv - Microbiology 2020Quote: ... we used 15 μL of a mixture of 4’,6-diamidin-2-phenylindole (4 μg mL−1) in SlowFade Gold Antifade Mounting medium (both Thermo Fisher Scientific, Waltham, MA, USA). All solutions and buffers for virus-targeted genomeFISH experiments were prepared with molecular grade water (Carl Roth ...
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bioRxiv - Plant Biology 2020Quote: Lines with defective stomatal responses to at least one stimulus were subjected to sequencing of “usual suspects” either by NGS-based sequencing of PCR amplicons obtained with the use of gene-specific primers (Supplementary Data Set 2) and Phusion DNA polymerase (ThermoFisher Scientific; ROUND 1 to 6), or whole-genome sequencing (ROUND 7 and 8) ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by 4’,6diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) staining for 10 min ...
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bioRxiv - Neuroscience 2021Quote: ... 2°: goat anti-mouse AF488 (1:500) (Invitrogen, A-11001), and donkey anti-rabbit Cy5 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and ethidium homodimer-1 (2 mM in DMSO, L3224, ThermoFisher) were added directly to the cell medium to a final concentration of 1 μM each ...
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bioRxiv - Neuroscience 2021Quote: ... stained with Neurotrace for 2 hours (1:500, N21479; Invitrogen), rinsed twice with 2x SSCT and mounted on a slide with Fluoromount-G ...
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bioRxiv - Microbiology 2019Quote: ... N-2 supplement (1:200; catalog number 17502-048; Invitrogen), 20 ng/ml fibroblast growth factor (catalog number 4114-TC-01M ...
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bioRxiv - Molecular Biology 2020Quote: ... and HA tag (2-2.2.14; ThermoFisher Scientific 26183, 1:10,000), used with goat anti-mouse IgG AlexaFluor®568 (ThermoFisher Scientific A-11004 ...