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Citations for Thermo Fisher :
1701 - 1750 of 10000+ citations for 6 Boc 1 oxa 6 azaspiro 3.3 heptan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, 62248). The percentage of infected cells at each time point was quantified using ImageJ software.
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bioRxiv - Molecular Biology 2023Quote: ... Nuclei were stained with DAPI (4′,6-diamidino-2-phenylindole) (Thermo Fisher Scientific). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... nuclei were stained with DAPI (4’,6-diamino- 2-phenylindole, dihydrochloride, Thermofisher, #D3571) solution (0.5 mg/ml) ...
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bioRxiv - Microbiology 2023Quote: ... 4 and 6 hpi RNA was extracted from cells using TRIzol (Invitrogen; 15596026). RNA was isolated and precipitated following the manufacturer’s institutions and 200ng was reverse transcribed into cDNA using the ABI cDNA synthesis kit (Applied Biosystems ...
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bioRxiv - Genetics 2023Quote: ... Taqman reactions were performed on a QuantStudio 6 qPCR machine (Thermo Fisher, #A43168) following manufacturer instructions using ∼25ng of DNA ...
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bioRxiv - Genetics 2023Quote: ... All samples were run on a QuantStudio 6 qPCR machine (Thermo Fisher, #A43168). CT values were normalized to standard curves generated from the BAC clones ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific). The preparations were analyzed under a confocal microscope Zeiss 510 LSM META and Zeiss 780-NLO (Carl Zeiss Microscopy ...
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bioRxiv - Cell Biology 2023Quote: ... and 6-diamidino-2-phenylindole (DAPI) were obtained from Invitrogen (Carlsbad, CA, USA). General laboratory chemicals and biochemicals ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2023Quote: ... PDiPSCs were first plated on gelatin coated 6-well plates (150239; Thermo Fisher) or 8-well cell culture slides (CCS-8 ...
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bioRxiv - Microbiology 2023Quote: ... Univ.F(G)+6 primers and Univ.R primers and SuperScript III Platinum kit (ThermoFisher) [51 ...
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bioRxiv - Neuroscience 2024Quote: ... All sections were counterstained with 4’,6’-diamidino-2-phenylindole (DAPI) (Thermo Fisher) before mounting.
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bioRxiv - Neuroscience 2024Quote: ... 6 μl of PowerUp™ SYBR™ Green Master Mix (Applied Biosystems, # A25742) and 3 μl of PCR grade water ...
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bioRxiv - Neuroscience 2024Quote: ... TNF alpha and IL-6 were quantified using the Procartaplex assays from Invitrogen. To assess functionality of KO in iPSC-derived myeloid cells ...
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bioRxiv - Neuroscience 2024Quote: ... Relative quantitation was determined using the QuantStudio 6 Flex detection system (Applied Biosystems) that measures real-time SYBR green fluorescence and then calculated by means of the comparative Ct method (2−ΔΔCt ...
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bioRxiv - Bioengineering 2024Quote: hMSCs were plated into a 6-well plate in antibiotic-free alphaMEM (Gibco) 24 h before siRNA transfection ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, 4485691). Fold change for each gene was determined using the 2UVV: ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were run on the QuantStudio 6 or 7 (ThermoFisher Scientific, Waltham, MA) using the qPCR reaction settings as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Cell nuclei were labeled with dapi (4 ’, 6-diamidino-2-phenylindole, Invitrogen, P36931). Images were obtained in the Zeiss LSM 800 Confocal Optical Microscope at Centro de Micro y Nanoscopía de Córdoba (CEMINCO-CONICET-UNC ...
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bioRxiv - Immunology 2024Quote: ... tibia and iliac bones from C57BL/6 mice were flushed with RPMI (Gibco) and red blood cells were lysed with ACK buffer (Gibco) ...
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bioRxiv - Biochemistry 2024Quote: ESAT-6 was buffer exchanged into PBS using a Zeba spin column (ThermoFisher) and diluted to a concentration of 50 µM ...
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bioRxiv - Physiology 2022Quote: ... the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG; 6.83 μg/kg BW, Invitrogen) dissolved in 50% dextrose (1 g/kg BW ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated for 30min at 37°C with 150µM of 2-[N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose (ThermoFisher).
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Cancer Biology 2021Quote: Feeder-free iPS cell culture was maintained on matrigel coated 6-well plates and routinely passaged with 1 ml accutase or 0.5 mM EDTA (Thermo Fisher Scientific). To enhance single cell survival ...
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bioRxiv - Developmental Biology 2021Quote: ... and gene primers (Supplementary table 1) were used to perform qPCR on QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher) and normalized to housekeeping gene Tbp ...
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bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
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bioRxiv - Immunology 2019Quote: ... DRGs inside vertebral column and the depilated hairy skin from PFA-perfused animals (6-12 weeks) were postfixed with 4% PFA for 1 hr or Zamboni fixative (Fisher Scientific) overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
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bioRxiv - Neuroscience 2020Quote: ... with specific primer pairs (table 1) were used for all qPCR assays and analyzed via QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Technical and experimental triplicates were performed for all samples ...
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bioRxiv - Cell Biology 2021Quote: ... with 0.4 µm pore size in 6 well plates with 1 ml Slice Culture Medium as follows: 50% MEM with GlutaMAX (Gibco, 41090036) 18% EBSS ...
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bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
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bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
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bioRxiv - Genetics 2022Quote: ... Cells (1-2.5 x106/mL) were then cultured at 37 °C ex vivo for 2-6 hours in RPMI (Gibco, 21875-034) containing 10% FCS (IGC Technical Services ...
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bioRxiv - Microbiology 2022Quote: Adenovirus containing the MCMV IE1 gene was generated by transfecting purified pAd5-MCMV_IE1 into 293A cells in 1 well of a 6 well plate using Lipofectamine 3000 (ThermoFisher, Waltham, MA) according to manufacturer’s instruction ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were centrifuged at 135 x g for 1 minute and resuspended in 6 ml of hepatocyte media (William’s E media (Gibco, 22551-022) with 3% fetal bovine serum (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
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bioRxiv - Cancer Biology 2019Quote: ... VM-CUB1 and RT-112 cells were seeded at 1 × 105 cells per well in 6-well plates along with siRNA duplexes or miRNAs using Lipofectamine RNAiMAX Reagent (Life Technologies). At 72 hours after transfection ...
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bioRxiv - Synthetic Biology 2020Quote: ... 2 µL of 1.25 µM 6-FAM-labeled ADO-22 primer and 1 µl of 100 mM ddTTP (USB-Affymetrix, Cleveland, OH). Fluorescently-labeled cDNA fragments were analyzed by capillary electrophoresis (CE) ...
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bioRxiv - Immunology 2019Quote: Fabs from rabbits were expressed in 293F cells for 6 days and then affinity purified using a CaptureSelect™ CH1-XL Pre-packed 1 ml Column (ThermoFisher).
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bioRxiv - Cancer Biology 2020Quote: ... and 60°C for 1 min.) using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). A dissociation curve was generated afterwards by ramping the temperature from 60°C to 95°C to determine the specificity of the PCR product ...
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bioRxiv - Genetics 2020Quote: 3×105 HeLa cells were seeded to each well of 6-well plates and cultured in DMEM supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Life Technologies) 24 hours before transfection ...
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bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
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bioRxiv - Genetics 2020Quote: ... All organoids were passaged and split once a week 1:6 and filtered through a 40 micron cell strainer (ThermoFisher scientific) to remove differentiated structures from the culture.
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bioRxiv - Cell Biology 2019Quote: ... then further cleared by centrifugation at 4°C and 25658 g for 1 h (Thermo Fisher Scientific, #A23-6×100 rotor). The supernatant was passed through a 0.22 µm filter (Corning #431097 ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
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bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...