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Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... slides were stained in 300 nM 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, D1306) and mounted in ProLong Diamond antifade mountant (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2ng/ml; Molecular Probes). Sections were mounted using ProLong Gold anti-fade reagent (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were counterstained with 4’,6-diamino-2-phenylindole (Prolong Gold antifade reagent DAPI; Invitrogen). The primary antibodies were vimentin ...
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bioRxiv - Biochemistry 2023Quote: ... at 4°C before centrifugation and bead removal using a DynaMag™-2 Magnet (Invitrogen). Supernatants mixed 1:1 with Co-IP elution buffer (2X NuPAGE® LDS ...
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bioRxiv - Cell Biology 2024Quote: ... Either 4 nM WR99210 (Jacobus Pharmaceuticals) or 2 μg/mL blasticidin S (Life Technologies, USA) was used for selecting transfectants ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis was evaluated thanks to 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, USA) and Annexin-V-APC (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were incubated 2-4 hrs in Earle’s balanced salt solution (EBSS; Gibco, # 24010043). Bafilomycin A1 (referred as BafA1 ...
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bioRxiv - Immunology 2024Quote: ... and stained with 200 ng/mL 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific, 62247) for 2 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... 1491-9 and 1861-10 were maintained in DMEM supplemented with 10% FBS (ThermoFisher Scientific) (19) ...
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bioRxiv - Molecular Biology 2021Quote: ... the cells were transiently transfected with HA-Flag-GHRHR-Rluc8 or HA-Flag-SV1-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using Lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were transiently transfected with HA-GLP-1R-Rluc8 and β-arrestin 1/2-Venus at a 1:9 mass ratio using lipofectamine 3000 reagent (Invitrogen) and cultured for another 24 h ...
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bioRxiv - Molecular Biology 2021Quote: The cDNA reactions were diluted 1:50 and 4 µL was used per 10 µL qRT-PCR reaction with PowerUp SYBR Green Master Mix (ThermoFisher, A25742) and final primer concentrations of 500 nM in 384-well plates (ThermoFisher ...
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bioRxiv - Bioengineering 2020Quote: ... Biotinylated mPA was then modified with 10 equivalents of sulfosuccinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate (sulfo-SMCC; Thermo Fisher Scientific) at r.t ...
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bioRxiv - Microbiology 2020Quote: GTPases at a final concentration of 10 μM were loaded with sub-stoichiometric (1/4 [GTPase]) concentrations of GDP-BODIPY-FL (Thermo Scientific) in the presence of 50 mM Tris pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... the beads were separated from the lysate and washed four times with 1 ml of CoIP buffer before resuspending in 15 μl of 1 x loading buffer (NuPAGE LDS sample buffer (4 x) and NuPAGE sample reducing agent (10 x) (ThermoFisher Scientific) diluted to 1 x with H2O) ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... 10 x 250 mm) at a flow rate of 4 ml min−1 using a Dionex UltiMate 3000 system (Thermo Scientific) fitted with a UV detector optimized to detect the O-phenyl functional group of the acceptor molecule at 270 nm ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells were passaged 1:4 into a new 15 cm dish and grown in DMEM supplemented with 10% FBS (Thermo Fisher) and Hygromycin (Thermo Fisher ...
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bioRxiv - Genomics 2022Quote: ... Sorted cells were centrifuged at 300g for 10 minutes at 4°C and resuspended in 1 ml of TRIzol (Invitrogen, 15596018). Spermatogonial RNA was purified according to the standard TRIzol protocol and contaminant genomic DNA was digested using the DNA-free kit (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... blocked again for 2h (10% CAS-Block / 90% PBT) and incubated overnight at 4°C with secondary antibody (1:250, Alexa-Fluor-594, ThermoFisher A32742) diluted in 100% CAS-Block ...
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bioRxiv - Pathology 2023Quote: ... 10 µg/L Amphotericin B (Gibco™ -15290026), 100 IU/mL Streptomycin (Gibco™-15140148 ...
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bioRxiv - Bioengineering 2022Quote: ... pulsing 2 s on and 4 s off for a total of 10 min of “on” time (Fisher Scientific, catalog no. FB120A220, probe CL-18). Note that from harvesting onward the libraries were kept at 4 °C ...
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bioRxiv - Microbiology 2023Quote: Epimastigotes and trypomastigotes lysates containing 2 x 107 parasites in 20 µL of SDS-PAGE sample buffer containing 5 mM DTT were boiled for 5 min and loaded onto precast Novex Value 4-12% Tris-Glycine gels (Thermo Fisher). The electrophoresis was performed in 50 mM MOPS-50 mM Tris Base ...
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... co-transfection of pX-CRISPR and qTAG repair plasmids was performed at a 1:1 ratio using 4 μg of total DNA (2 μg cut + 2 μg repair) in Opti-MEM Reduced Serum Medium (31985070, Gibco) with Lipofectamine 3000 (L3000008 ...
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bioRxiv - Physiology 2019Quote: Total RNA samples were extracted from one thousand 4-7 day-old Culex female antennae with TRIzol reagent (Invitrogen, Carlsbad, CA). Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... were added and incubated for 4 hours at 4°c followed by one wash with lysis buffer (Pierce) with added Halt protease and phosphastase inhibitors (Thermo Scientific) and two washes with PBS and resuspended in PBS for Mass spectrometry.
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bioRxiv - Biochemistry 2023Quote: ... A drop of 3.5-4 µl sample was applied on one side in a Vitrobot (Vitrobot Mk IV, Thermo Fisher Scientific) at 4 °C and 100 % humidity ...
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bioRxiv - Microbiology 2023Quote: We diluted freshly purified virus samples in DPBS and combined three volumes of the virus with one volume of NuPAGE LDS Sample Buffer (4×; Thermo Fisher) containing 100 mM dithiothreitol ...
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bioRxiv - Developmental Biology 2019Quote: ... with successfully electroporated embryos fixed using 4% Paraformaldehyde (PFA; Fisher Scientific, 30525-89-4) at 4°C overnight.
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bioRxiv - Physiology 2019Quote: TT’s were visualized by staining with di-4-ANEPPS (4 µmol/l. Invitrogen, UK) as described in detail previously 8,32,70 ...
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bioRxiv - Cell Biology 2022Quote: ... with an Eagle 4 K x 4 K CCD camera system (Ceta CMOS, ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... 4 µg protein was resolved on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher) and transferred to PVDF membrane (Fisher) ...
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bioRxiv - Immunology 2022Quote: ... Hemocytes were fixed in 4% PFA (4% in PBS; J19943-K2, Thermo Fisher Scientific) for 30 min at RT ...
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bioRxiv - Molecular Biology 2021Quote: ... Thawed CM aliquots were mixed 1:1 with basal media (BM) consisting of 2% B-27 (Gibco 12587001), 10 mM Nicotinamide (Sigma-Aldrich N0636) ...
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bioRxiv - Neuroscience 2024Quote: ... Wisteria floribunda lectin (1:1000, B-1355-2, Vector) was visualized using Streptavidin 568 (1:500, S11226, Invitrogen).
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bioRxiv - Cancer Biology 2020Quote: ... incubated 5-10 minutes in DRAQ5 (1/1000, Thermofisher) for DNA staining and finally washed in PBS ...
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bioRxiv - Neuroscience 2021Quote: ... followed by 5 min incubation with 500 µL DPBS plus 4 µM Hoechst 33342 (Life Technologies). Images were acquired using an Eclipse Ti2-E epifluorescence microscope (Nikon ...
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bioRxiv - Immunology 2021Quote: ... for 20 min at 4°C or with 5 ng/mL of BODIPY 500/510 (Invitrogen). Cells were washed with PBS and immediately analyzed.
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bioRxiv - Plant Biology 2021Quote: ... 0.3-5 µg of total RNA was treated with 4 U of TURBO DNase (Life Technologies) in 2 consecutive incubation steps ...
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bioRxiv - Microbiology 2020Quote: ... pA1* cells were treated with 500µM of the protonophore TCS (3,3’,4’,5-Tetrachlorosalicylanilide; Fisher scientific). The fluorescent signal from at least 100,000 individual bacteria per condition was measured by flow cytometry with a MCSQuant® VYB analyzer (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2022Quote: ... Running conditions used were 4–12% Bis-Tris gel in 5% MOPS Running Buffer (Invitrogen, NP0001) at 200 V for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen) at 2 mg/ml in PBS for 10 min or with Laurdan (see above ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Total RNA was isolated from liver tissues (n=4-5 mice/group) using Trizol (ThermoFisher Scientific). Concentration was measured by nanodrop (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were incubated for 5 min at 65°C and 4 ml 5x FSB buffer (Invitrogen), 1 ml murine RNase inhibitor ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) (Thermo Scientific) and 1 mM isopropyl beta-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 5 min at 37 °C with 4 ml pre-warmed TrypLE 1X Express (Gibco), pipetted off the cell culture dish ...
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bioRxiv - Cell Biology 2021Quote: ... incubated for 5 min at 37 °C with 4 ml pre-warmed TrypLE 1X Express (Gibco), quenched with pre-warmed growth medium ...
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bioRxiv - Cell Biology 2021Quote: ... and boiled for 5 minutes before fractionating on a 4–12% NuPAGE Bis-Tris gel (Invitrogen). Proteins on a gel were transferred to a nitrocellulose membrane and analyzed by using the antibodies at 1:3000-10,000 dilutions ...