Labshake search
Citations for Thermo Fisher :
1651 - 1700 of 10000+ citations for 6 Methyl 2 Mercaptobenzothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... in a thermal cycler (QuantStudio 6 Flex, Applied BioSystems) according to the manufacturer’s instruction (BioRad) ...
-
bioRxiv - Genetics 2023Quote: ... This NIM consisted of Essential 6 medium (Gibco #A1516401) supplemented with Dorsomorphin (Sigma Aldrich #P5499-5MG ...
-
bioRxiv - Biochemistry 2023Quote: Each plate (6-well or 10 cm, Fisher Scientific) of HEK293T cells at ∼70% confluency was transfected with plasmids encoding ‘POIN-CAGEN’ and ‘CAGEC-POIC’ with Lipofectamine 2000 (Thermo Fischer ...
-
bioRxiv - Molecular Biology 2023Quote: ... The different TMT-6 plex labels (Thermo Fisher Scientific) were dissolved in 41µL of anhydrous acetonitrile ...
-
bioRxiv - Plant Biology 2023Quote: ... 6 mg Dynabeads Protein A (Thermo Fisher Scientific K.K.) was added to the supernatant and incubated on ice for 30 min ...
-
bioRxiv - Plant Biology 2023Quote: ... 6 mg Dynabeads Protein A (Thermo Fisher Scientific K.K.) was added to the supernatant and incubated on ice for 30 min ...
-
bioRxiv - Cell Biology 2023Quote: ... in 6 well plates and mTeSR8 medium (Gibco, A1516901) supplemented with 100 U/mL penicillin/Streptomycin ...
-
bioRxiv - Cell Biology 2023Quote: ... and into pcDNA™6/myc-His A (Invitrogen), from an amplicon produced by RT-PCR from S2Xpress RNA ...
-
bioRxiv - Cell Biology 2023Quote: ... supplied with 6 µL Lipofectamine 2000 transfection reagent (Invitrogen) and 1-2 µg plasmids for 2 h ...
-
bioRxiv - Immunology 2023Quote: ... on a QuantStudio 6 Flex PCR system (ThermoFisher Scientific) with the primer pairs listed in Supplementary Table 1 ...
-
bioRxiv - Developmental Biology 2023Quote: ... with a 6-tube magnetic stand (Thermo Fisher #AM10055) used to secure beads between washes ...
-
bioRxiv - Cancer Biology 2022Quote: ... BMF: Hs00372937_m1,) on a QuantStudio 6 instrument (Thermo Fisher). Differential gene expression was calculated using the 2−ΔΔCt method with GAPDH as a housekeeping control gene in three technical replicates.
-
bioRxiv - Molecular Biology 2023Quote: ... 20 µl of 6 µM CCF4- AM (ThermoFisher Scientific) in Blam loading solutions (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2023Quote: ... 6-well glass bottom plates (Thermo Scientific, #NUNC-140675) were coated with Poly-L-Lysine 0.01% (v/v ...
-
bioRxiv - Cell Biology 2023Quote: ... 6-well glass bottom plates (Thermo Scientific, #NUNC-140675) were coated with Poly-L-Lysine 0.01% (v/v ...
-
bioRxiv - Biochemistry 2023Quote: ... 6 μL Gateway LR Clonase II enzyme mix (ThermoFisher), TE buffer to 30 μL ...
-
bioRxiv - Cell Biology 2023Quote: ... They were transfected with Rme-6 siRNA (GGAGAAUUCUUGAGUCGAUtt; ThermoFisher) or non-targeting siRNA (final concentration 200nM ...
-
bioRxiv - Cancer Biology 2023Quote: ... on a QuantStudio™ 6 Realtime PCR System (ThermoFisher). Following qPCR quantification ...
-
bioRxiv - Neuroscience 2023Quote: ... Human IL-6 ELISA kit was from Life Technologies. FAN1KO HD patient fibroblasts were generated and received from Synthego ...
-
bioRxiv - Developmental Biology 2023Quote: ... in a QuantStudio 6 Flex system (Thermo Fisher Scientific). The Ct cycles were normalized to the Actb gene ...
-
bioRxiv - Cell Biology 2023Quote: NPCs were cultured in 6-well (Fisher Scientific Corning) or black 96-well clear bottom (Fisher Scientific Corning ...
-
bioRxiv - Zoology 2024Quote: ... 6 μL of DreamTaq PCR Master Mix (Thermo Scientific) and 2,9 μL of nuclease-free water ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... FLIPR Calcium 6 Evaluation Kit (Molecular Probes, Oregon, US); PathHunter® Detection Kit (DiscoverX ...
-
bioRxiv - Neuroscience 2024Quote: ... or Cellbind plates (6 or 96 well, Fisher Scientific) depending on the experiment.
-
bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Mature sensory neurons in 8-well μ-slide chambers were co-stained with 10 nM tetramethylrhodamine methyl ester (TMRM; Life Technologies, Grand Island, NY) and 100 nM MitoTracker Green FM (Life Technologies ...
-
bioRxiv - Neuroscience 2023Quote: All fly lines were maintained in mixed sex groups in bottles on JazzMix media or our own food made following the same recipe (brown sugar, corn meal, yeast, agar, benzoic acid, methyl paraben and propionic acid; Fisher Scientific, Whitby, ON, Canada) at 25°C ...
-
bioRxiv - Biochemistry 2023Quote: ... Each well was then further passivated by adding 9 μL of a freshly prepared aqueous solution of methyl-PEG4-NHS-Ester (10 mg/mL, Thermo Fisher, Cat. No. 22341), followed by adding 1 μL of 1 M NaHCO3 (pH 8.5) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Firstly proteins were precipitated with 50 μL of acetonitrile followed by steroid extraction via liquid-liquid extraction with 1 mL tert-methyl butyl ether (MTBE, Acros Organics, Fisher Scientific, UK). The MTBE layer was then removed ...
-
bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
-
bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
-
bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
-
bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
-
bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
-
bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
-
bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
-
bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
-
bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
-
bioRxiv - Molecular Biology 2021Quote: Mitochondrial membrane potential of primary neurons was measured by fluorescent staining of Tetramethylrhodamine methyl ester (Image-iT™ TMRM Reagent, ThermoFisher Scientific, Waltham, MA, USA) according to the manufacturer’s instructions.
-
bioRxiv - Immunology 2022Quote: ... The mitochondrial membrane potential and dysfunctional mitochondria were measured by staining BM cells with Tetramethylrhodamine Methyl Ester (TMRM 500 nM, Thermo Fisher Scientific, Cat. No. T668), MitoTracker Green (100 nM ...
-
bioRxiv - Biochemistry 2022Quote: ... 2 mM Tris(2-carboxyethyl)phosphine (TCEP, ThermoFisher)) supplemented with a protease inhibitor tablet (Roche ...
-
bioRxiv - Genetics 2019Quote: ... 2 μl of probe (2 μM; Applied Biosystems) and 2 μl of template DNA (where extracts were normalised to 34 ng/μl) ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA was extracted from the amygdala of female offspring at P7 and P90 (n=6/mCHD, n=6/mHFD) using TRIzol Reagent (15596018; ThermoFisher Scientific, Ottawa, ON, CAD) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2020Quote: ... or 52- or 78-week-old C57BL/6 mice or C57BL/6-TgTM4SF5 mice by perfusion of the liver using collagenase type II (Life Technologies, Carlsbad, CA, USA) (Ateş et al ...
-
bioRxiv - Immunology 2023Quote: ... Monocytes were harvested from 6-week-old C57BL/6 mice and were differentiated into dendritic cells using supplemented culture medium: RPMI 1640 (Life Technologies, Waltham, Massachusetts, USA), 10% HIFBS (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...
-
bioRxiv - Molecular Biology 2020Quote: ... 2 (Thermofisher). A reverse transfection approach was used to deplete AurkA and CEP135 ...
-
bioRxiv - Systems Biology 2023Quote: ... 2% (ThermoFisher). Purified amplicons were quantified by Qubit™ dsDNA Quantification Assay Kits (ThermoFisher ...