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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Tert Butyldimethyl 3 4 4 5 5 Tetramethyl 1 3 2 Dioxaborolan 2 Yl Phenoxy Silane since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... then 5 μg were loaded and separated on 4–12% NuPage acrylamide gels (Invitrogen) with NuPage MOPS running buffer for 2 h at 110 V ...
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bioRxiv - Microbiology 2021Quote: ... Lysates containing around 5 × 106 cells were separated on 4-20% polyacrylamide gels (Invitrogen) in running buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... the premixed solution containing 4 µl of 5×RT buffer (Invitrogen, Cat no. #18080044), 2µl of 0.1 M DTT (kit component of Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... HIOs were cultured in group of 5 per well using 4-well plates (ThermoFisher). Lumens of individual HIOs were microinjected with glass caliber needles with 1 μl of PBS or different strains of Salmonella (105 CFU/HIO or 103 CFU/HIO for 24h time point experiments) ...
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bioRxiv - Immunology 2021Quote: ... 94°C 5 min) with 4 μl of RNA and random hexamers (Thermofisher scientific). A PCR was further performed based on the protocol established by Tiller et al (Tiller et al. ...
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bioRxiv - Neuroscience 2022Quote: ... were passaged every 4-5 days using Stem-Accutase (Cat# A11105-01, Life Technologies) in the presence of 1 µM Thiazovivin (THZ Cat# SML1045-25 MG ...
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bioRxiv - Microbiology 2022Quote: ... rabbit anti-TLR-4 polyclonal antibody (5 µg/ml, PA5-23124, Thermo Fisher Scientific), and rabbit anti-TLR-5 polyclonal antibody (10 µg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... Sections of 4-5 µm were cut using a rotary microtome (HM355S, Thermo Scientific). Sections were deparaffinized in xylene and rehydrated in a descending series of ethanol (96%–50% ...
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bioRxiv - Microbiology 2024Quote: ... After 4–5 days of culture in the Expi293 Expression Medium (Thermo Fisher Scientific), supernatants were collected and passed through a 0.22-µm filter ...
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bioRxiv - Physiology 2024Quote: ... Intracellular Ca2+ ([Ca2+]i) measurements were conducted using Fluo-4/AM (5 µM; Invitrogen)-loaded cells ...
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bioRxiv - Neuroscience 2024Quote: ... the reaction was quenched with 4 μl of 5% hydroxylamine (Termo Fisher Scientific, 90,115) for 15 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged as small clumps every 4 to 5 days with Dispase (Gibco). All cells were cultured at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... 94°C 5 min) with 4 μl of RNA and random hexamers (Thermofisher scientific). A PCR was further performed based on the protocol established by Tiller et al (Tiller et al ...
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bioRxiv - Neuroscience 2022Quote: ... the reaction was quenched with 4 μl of 5% hydroxylamine (Thermo Fisher Scientific, 90115) for 15 min ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 5 µg/ml NeutrAvidin (Thermo Fisher Scientific), then blocked in PBS containing 1% BSA for 1 hour at room temperature (RT) ...
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bioRxiv - Biochemistry 2023Quote: ... 5-7 μg BACMID DNA was incubated with 4 μL Fugene (ThermoFisher, Cat# 10362100) in 200 μL of ESF 921 media for 30 minutes at 230C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... muscles were injected with 5 µM Fluo-4 penta-potassium salt (ThermoFisher Scientific, USA) as previously described and viewed with DIC optics on a Nikon Eclipse TE300 inverted light microscope (400× ...
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bioRxiv - Developmental Biology 2023Quote: ... Sections of 4-5 μm were cut using a rotary microtome (HM355S, Thermo Scientific). Sections were deparaffinized in xylene and rehydrated in a descending series of ethanol (96%–50% ...
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bioRxiv - Cell Biology 2023Quote: ... Medium was exchanged on DIV 4-5 for phenol-free Neurobasal medium (Thermo Fisher) supplemented with GlutaMAX 1x (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were cultured at 37 °C and 5 % CO2 on 4-well plates (Nunc) (300 µl/well ...
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bioRxiv - Neuroscience 2023Quote: ... the reaction was quenched with 4 μl of 5% hydroxylamine (Thermo Fisher Scientific, 90115) for 15 minutes ...
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bioRxiv - Biophysics 2024Quote: ... Cells were incubated with 5 µM Fluo-4-AM (Thermo Fisher scientific, Waltham, MA) along with 0.1% pluronic F-127 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... 7.3) containing 5 μM of the cytosolic Ca2+ indicator Fluo-4 AM (Invitrogen, Switzerland) solubilized in Krebs solution (in mM ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Biophysics 2021Quote: ... Protein samples (12 μL) were mixed with 3 μL 5 M DTT and 5 μL 4x NuPAGE™ LDS sample buffer (Thermo Fisher Scientific) and incubated (95 °C ...
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 3.5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Biophysics 2023Quote: ... The grids were blotted for 5 s with 3 force after waiting for 5 s and immersed in liquid ethane using Vitrobot (Mark IV, Thermo Fisher Scientific/FEI) in condition of 100% humidity and 8°C.
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Molecular Biology 2024Quote: ... co-transfection of pX-CRISPR and qTAG repair plasmids was performed at a 1:1 ratio using 4 μg of total DNA (2 μg cut + 2 μg repair) in Opti-MEM Reduced Serum Medium (31985070, Gibco) with Lipofectamine 3000 (L3000008 ...
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bioRxiv - Neuroscience 2024Quote: Intracellular calcium imaging was carried out on isolated taste bud cells loaded with 4 µM of the ratiometric calcium indicator Fura-2-acetoxymethyl ester (Fura-2 AM, Invitrogen) in Tyrode’s with 0.05% pluronic acid F-127 (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... Cultured colonies from organoids were fixed with 4% PFA for 2-4 hrs at room temperature followed by immobilization with Histogel (Thermo Scientific) for paraffin embedding ...
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 mm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive ...
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bioRxiv - Systems Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 μm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive high-performance benchtop quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... sham and uPA-CBP were anesthetized (4% isoflurane, 2 liters of oxygen) and transcardial perfusion fixed with 4% paraformaldehyde (PFA) (Cat #50-980-494, Fisher Scientific) in PBS 1x until stiff ...
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bioRxiv - Developmental Biology 2024Quote: ... with a Dionex IonPac AG11-HC-4 μm guard column (2 mm i.d × 50 mm, 4 μm particle size, Thermo Fisher Scientific) and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm ...
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bioRxiv - Developmental Biology 2024Quote: ... and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... then loaded with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethylester (BCECF-AM, 1.6 μM, Life Technologies) for 30 min at 37°C ...
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bioRxiv - Pathology 2022Quote: ... as above and left to attach for 2 hours before adding bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrDU, Invitrogen B23151) to a final concentration of 75 µM and left to proliferate for 6 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Samples were washed at least 5 times 20 min and transferred to 0.25% PBT with 1:400 TO-PRO-3 (ThermoFisher #T3605) for 2 nights ...
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bioRxiv - Bioengineering 2022Quote: ... cells were washed 3 x 5 min with PBS before adding secondary antibody (1:200 Alexafluor-488 goat anti-mouse, Invitrogen) and rhodamine phalloidin (1:100 ...
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bioRxiv - Neuroscience 2021Quote: ... Then slides were washed 3×5’ in TBS and incubated in donkey-α-sheep-488 (1:500, Life Technologies, A11015) in TBS for 90’ ...
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bioRxiv - Developmental Biology 2020Quote: ... Slides were then washed with PBS-T (3 × 5 min) and incubated with fluorophore-conjugated secondary antibodies (1:500 in blocking buffer, Invitrogen). Hoechst 33258 (Invitrogen ...