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Citations for Thermo Fisher :
1601 - 1650 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (hi-FBS, Gibco, 16140-071), 100U/mL Penicillin/100ug/mL Streptomycin (pen/strep ...
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bioRxiv - Plant Biology 2022Quote: ... the secondary antibody used was anti-6x-His tag monoclonal (Invitrogen, # MA1-21315) with 1:100 dilutions ...
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was then purified using Ni-NTA His affinity agarose (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6x-His Epitope Tag Monoclonal Antibody (#MA1-21315, Thermofisher Scientific; dilution 1:500) was used for His-tag staining of the TRPA1 channel in fixed cells for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... excluding the HI-FBS and supplementing with 0.1% BSA (Life Technologies 15260-037). CHIM was used for the experiments in Figures 4 ...
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bioRxiv - Microbiology 2024Quote: ... Bio-His-FIKK13 was incubated with different quantities of magnetic streptavidin beads (Invitrogen) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with an anti-His antibody with HRP conjugate (Invitrogen) in 1% BSA at 4°C overnight with gentle rocking ...
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bioRxiv - Genetics 2023Quote: ... Libraries were mixed with Novex 5X Hi-Density TBE Sample Loading Dye (Thermofisher), then loaded into a 6% TBE acrylamide gel with the Quick-Load pBR322 DNA-MspI Digest ladder and run for 65 minutes at 120 V ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Western blots were carried out probed with anti-His mAb (1:1000, Invitrogen) followed by anti-mice IgG-HRP (1:20000 ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed using anti-His antibody (Thermo Fisher, Cat. No. MA1-21315) at a dilution of 1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... neutralized by adding RPMI-1640 with 10% heat inactivated FBS (HI FBS, Gibco), and spun down at 200 x g for 10 minutes to pellet the cells ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Synthetic Biology 2024Quote: ... then diluted at 1:100 in Hi-Def Azure Media (Fisher Scientific # 3H5000) supplemented with 1% Glucose and various concentrations of peptide in 100 µl in a 96well plate ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were placed in HI buffer containing 1:1,000 CellMask Deep Red (Invitrogen) for 15 minutes at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Developmental Biology 2024Quote: Primary antibodies used were HRP-conjugated anti-His (Invitrogen 46-1009; 1:2000) and mouse anti-FLAG (Sigma F1804 ...
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bioRxiv - Molecular Biology 2024Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Molecular Biology 2020Quote: ... 5ug of total RNA was biotinylated using the Click-IT kit (ThermoFisher Scientific, #C10365), per the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: Biotinylated nascent RNAs were bound to 30 μL Dynabeads MyOne Streptavidin C1 (Invitrogen and) washed 3 times as described in 40 and purified with TRIZOL reagent.
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bioRxiv - Molecular Biology 2020Quote: ... The RNA was biotinylated by adding 200 μl Biotin-HPDP (Thermo Fisher Scientific #21341) dissolved to 1 mg/ ml in 30% DMF ...
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Direct analysis of ribosome targeting illuminates thousand-fold regulation of translation initiationbioRxiv - Molecular Biology 2020Quote: ... and 3′ biotinylated using the Pierce RNA 3′end biotinylation kit (Thermo Scientific 20160).
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bioRxiv - Neuroscience 2021Quote: ... RNA was biotinylated using the PierceTM RNA 3’ End Desthiobiotinylation Kit (Thermo Fisher Scientific). 50 pmol of RNA was incubated in T4 RNA ligase buffer at 16 °C overnight ...
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bioRxiv - Genomics 2020Quote: ... Then the Biotinylated-DNA was captured by Dynabeads MyOne Streptavidin C1 beads (ThermoFisher #65001) in 1X BW buffer (5mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2020Quote: ... the biotinylated RNA was resuspended in water and separated with M280 Streptavidin Dynabeads (Invitrogen). 100 μl/sample of beads were washed twice with 2 volumes of freshly prepared wash buffer (100 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2019Quote: ... IgG was biotinylated by EZ-Link Sulfo-NHS-LC-Biotin kits (Thermo Fisher Scientific). Different amounts of biotinylated IgG was linked into RBCs through SA-biotin interaction at RT for 30 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Oligos were ethanol-precipitated and filled-in with 1 mM biotinylated dUTP (Thermo Fisher) using Klenow fragment (NEB) ...
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bioRxiv - Systems Biology 2019Quote: ... Biotinylated DNA was purified using Pierce Streptavidin magnetic beads (Thermo Fisher Catalog No. 88816). While on beads ...
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bioRxiv - Cell Biology 2019Quote: ... we first cover the epoxy slides with biotinylated BSA (1 µg/µl, Thermo Scientific). Streptavidin (0.5 µg/µl Neutravidin ...
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bioRxiv - Neuroscience 2019Quote: Biotinylated peptides were linked to StreptAvidin-and NeutrAvidin-Agarose (#29200 Thermo Scientific, Rockford IL) or SpeedBeed magnetic NeutrAvidin (GE Healthcare #78152104011150 ...
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bioRxiv - Cell Biology 2021Quote: ... followed by affinity precipitation of biotinylated molecules by streptavidin-conjugated Dyna beads (Thermo Fisher). The eluted samples were subjected to Western Blotting analyses using rabbit monoclonal anti-MT1-MMP antibody.
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bioRxiv - Cancer Biology 2019Quote: ... Biotinylated proteins were isolated by incubating the cell lysates with Avidin-coated beads (ThermoFisher) for 1h at 4°C ...
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bioRxiv - Bioengineering 2020Quote: ... biotinylated CD19 (10 µg / mL; Acro Biosystems #CD9-H8259) and Streptavidin-APC (ThermoFisher #S868) were used according to manufacturer instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Porcine mucosal heparin was biotinylated at the reducing end using hydroxylamine biotin (ThermoFisher, UK) as described by Thakar et al ...
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bioRxiv - Biophysics 2021Quote: ... the cells were incubated with a biotinylated secondary antibody (ThermoFisher Scientific 13-4013-85) at a dilution of 1:250 for two hours ...
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bioRxiv - Neuroscience 2020Quote: ... 2 μl biotinylated dextran amine 10,000 (BDA, Invitrogen, 10% wt/vol in sterile saline) was injected at the same coordinates as the AAVs into the left motor cortex six weeks after SCI ...
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Comparison of the neuraminidase antigenicity in recently circulating influenza A and vaccine virusesbioRxiv - Microbiology 2021Quote: Biotinylated fetuin was made using the EZ-Link NHS-PEG4-Biotinylation kit (Thermo Scientific). Briefly ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were probed with either biotinylated anti-C-tag conjugate (ThermoFisher, Cat. No. 7103252100) for the detection of baits ...
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bioRxiv - Microbiology 2020Quote: ... biotinylated cells were subsequently incubated with 0.1 mg ml-1 Dylight488-streptavidin (21832, Invitrogen) for 3 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and column purified RNA was biotinylated using Pierce RNA 3’ End Biotinylation Kit (ThermoFisher). Recombinant His-tagged NSP12 (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... Biotinylated proteins were then purified using high capacity neutravidin-conjugated beads (Thermo Fisher Scientific) via incubation for three hours at 4°C after which beads were washed extensively in dilution buffer plus 0.5M NaCl ...
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bioRxiv - Neuroscience 2021Quote: ... which was detected by incubating the sections with secondary biotinylated antibodies (Life Technologies™) for 2 h at room temperature and then with an avidin-biotin complex at 37°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Porcine mucosal heparin was biotinylated at the reducing end using hydroxylamine biotin (ThermoFisher, UK) as described by Thakar et al ...
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bioRxiv - Immunology 2022Quote: ... The biotinylated whole-cell lysate was then added to FITC neutravidin beads (ThermoFisher, F8776) at a ratio of 5µg antigen:1µL beads ...