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Citations for Thermo Fisher :
1451 - 1500 of 8720 citations for Recombinant Human LDLR His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... Biotinylated proteins were captured using Pierce Streptavidin Magnetic Beads (Thermo Fisher Scientific, 88817) at 4°C on a rotator for 3 h ...
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bioRxiv - Genomics 2022Quote: ... Biotinylated fragments were purified using the My T1 Streptavidin coated beads (Life Technologies) and subject to on-bead library preparation as described previously (Rao et al. ...
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bioRxiv - Immunology 2022Quote: ... was biotinylated using the EZ-link Sulfo-NHS biotinylation kit (Thermo Scientific, 2143) according to the protocol provided ...
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bioRxiv - Microbiology 2022Quote: ... biotinylated RNA was detected by DyLight™800-conjugated Streptavidin (Invitrogen, USA, Waltham) using the Odyssey CLx platform (LI-COR ...
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bioRxiv - Cancer Biology 2024Quote: ... Biotinylated proteins were detected with NeutrAvidin DyLight 800 (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Biophysics 2023Quote: ... Biotinylated fluoresceine isothiocyanate (b-FITC; 732.8 Da) was purchased from Thermo Scientific (# 10752905). A tandem repeat of the Z domain of protein A connected through a flexible spacer (12 amino acids ...
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bioRxiv - Immunology 2022Quote: ... B6 were biotinylated with EZ-link Sulfo-NHS-LC-LC-Biotin (Thermo Fisher) and excess biotin was removed using a Zeba size exclusion column (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... Biotinylated proteins were detected with streptavidin coupled to Alexa Fluor 594 (Thermo Fisher).
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bioRxiv - Immunology 2023Quote: ... The biotinylated proteins were tetramerized by adding APC-conjugated streptavidin (Thermo Fisher Scientific) at an allergen ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by capture of biotinylated DNA fragments using streptavidin beads (MyOne C1, Invitrogen). The second end of the captured DNA were repaired using an enzymatic cocktail (15 U T4 DNA polymerase ...
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bioRxiv - Cancer Biology 2023Quote: ... The biotinylated DNA fragments were purified using Dynalbeads M-280 streptavidin beads (Invitrogen) as outlined in the Illumina Mate-Pair protocol ...
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bioRxiv - Genomics 2023Quote: ... The slide surface was functionalized with biotinylated BSA (bBSA; ThermoFisher, 2 mg/mL) for 20-30 minutes and then washed for 5-10 minutes with 50 mM HEPES (pH 8.0) ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotinylated probes were detected using Chemiluminescent nucleic acid detection module (Thermo Scientific # 89880) following manufacturer’s protocol.
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bioRxiv - Genomics 2023Quote: ... were biotinylated using the EZ-Link™ Sulfo-NHS-LC-Biotin kit (ThermoFisher) as per the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... Fragmented biotinylated RNA was bound to the streptavidin magnetic beads (Thermo Fisher Scientific), washed ...
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bioRxiv - Molecular Biology 2023Quote: ... (37) using a complementary biotinylated probe JW32 (TTATGAGCCCGACGAGCTACCAGGCT-Biotin) and streptavidin agarose (Invitrogen). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated proteins were enriched using magnetic streptavidin beads (Dynabeads M-280, Invitrogen, USA). The beads were washed with a series of SDS buffer ...
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bioRxiv - Cell Biology 2024Quote: ... followed by biotinylated cDNA enrichment using Dynabeads MyOne Streptavidin C1 (Thermo Fisher Scientific). The 3′ cDNA adapter was ligated by T4 RNA ligase 1 (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... biotinylated Ube2d2S22R was immobilised onto Nunc MaxiSorp 96-well plates (Thermo Fisher Scientific) that were coated with streptavidin or neutravidin (New England BioLabs) ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated proteins were captured with Pierce™ Streptavidin Agarose (Thermo Fisher Scientific, 20353) on a rotator for 4 h at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... we injected 50 or 2×50 nl biotinylated dextrane amine (BDA-3000, Invitrogen) into the medial septal area (MS ...
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bioRxiv - Cell Biology 2024Quote: ... Biotinylated Rpl16a/b was detected by HRP-conjugated Streptavidin (1:10’000, ThermoFisher #N100).
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bioRxiv - Microbiology 2024Quote: ... . Biotinylated proteins were detected with streptavidin Alex Fluor 594 conjugate (Invitrogen; S-11227)
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bioRxiv - Synthetic Biology 2024Quote: ... and then biotinylated in-house using the EZ-Link Biotinylation kit (Fisher Scientific). After equilibrating tips in BLI buffer (1X PBS + 0.05% Tween-20 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Molecular Biology 2021Quote: ... We immunoprecipitated GFP-tagged Znf684 with 1 μg anti-GFP antibody (G10362, Life Technologies) overnight followed by a 2-hour incubation with Protein G Dynabeads (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR8-KLK4-KLKP1 (DDK tagged) was recombined with pLenti6/V5-Dest™ (Life Technologies) or pAD/CMV/V5-Dest™ (Life Technologies) ...
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... and/or AlexaFluor-594-tagged goat anti-guinea pig IgG (1:500; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated with the appropriate Alexa 546-tagged secondary antibody (Invitrogen, Carlsbad, CA) for 2 hrs at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged RNF219 and CNOT1 cDNAs were cloned into pCDNA5/FRT-TO vector (Invitrogen). The expression vectors were then transfected into HEK-293 Flp-In-TRex cells and followed by selection with hygromycin to generate stable cell lines ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-tagged GSK3β constructs were cloned into the pDEST™27 vector (ThermoFisher Scientific) as described previously66 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
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bioRxiv - Immunology 2022Quote: ... and RAW 264.7 EROS FLAG-tagged cells were maintained in DMEM medium (21969035, ThermoFisher) containing 10 % FBS (F7524 ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
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bioRxiv - Biochemistry 2023Quote: ... 6xHis-tagged CapZ was purified as previously described30,49 using HisPur Cobalt resin (Thermo Scientific), and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... A fluorescently tagged secondary antibody (Goat anti-rabbit, Alexa Fluor 647, Thermo Fisher Scientific) diluted in the same antibody solution was applied for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 488-tagged donkey-α-goat secondary antibody (1:1000; Molecular Probes) for one hour ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...
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bioRxiv - Cell Biology 2023Quote: ... and His6-tagged VASP (Benz et al., 2009) using Lipofectamine 2000 (Invitrogen, Karlsruhe, Germany). Cells were lysed in buffer A (40 mM HEPES-NaOH ...
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bioRxiv - Developmental Biology 2023Quote: ... The primary labelling was detected using appropriate Alexa-Flour tagged secondary antibody (ThermoFisher Scientific).