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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: The determination of serum TNF-α and IFN-γ concentration was performed using Mouse IFN gamma Uncoated ELISA and Mouse TNF alpha Uncoated ELISA (Invitrogen). The protocols recommended by the vendor were strictly adhered to.
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bioRxiv - Neuroscience 2020Quote: ... was added to the plate after which a colour reaction was generated by incubation with TMB (3,3’,5,5’-tetramethylbenzidine) colorimetric substrate (1-Step Ultra TMB-ELISA Substrate Solution, ThermoFisher Scientific). The reaction was stopped with 2 M H2SO4 and the plate read at 405nm with a Tecen Infinite M1000 plate-reader ...
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bioRxiv - Biochemistry 2022Quote: ... followed by three washes in 1% BSA and incubation with TMB-ELISA substrate (Thermo Fisher Scientific, Cat. no. 34028) until the light blue color appeared ...
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bioRxiv - Biochemistry 2021Quote: ... Binding was visualized through the addition of 50 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (ThermoFisher, #34028), incubated in the above plate shaker for approximately 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... A volume of 25 μL/well of 1-step Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, cat. # PI34029) was added to the plates and incubated for 5 to 10 min at RT ...
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bioRxiv - Immunology 2021Quote: ... and CXCL10 via enzyme linked immunosorbent assay (ELISA) (ThermoFisher). ELISA data was acquired on a SpectraMax M5 Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Neuroscience 2021Quote: ... 150uL room temperature TMB-ELISA reagent (Thermo Fisher, 34028) was added to each well ...
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bioRxiv - Bioengineering 2020Quote: ... Mouse IFN-γ Platinum ELISA (Invitrogen, Carlsbad, CA, USA) was performed on the supernatants.31
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bioRxiv - Microbiology 2021Quote: ... ELISAs were developed with ABTS solution (Thermo Fisher 002024) and absorbance was measured at 415 nm and 695 nm.
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... and incubated with Ultra TMB-ELISA Substrate Solution (ThermoFisher) for 20 min ...
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bioRxiv - Microbiology 2021Quote: ... 0.05% Tween20 TBST ELISA wash buffer (ThermoFisher Scientific; N503). Plates were coated for 2 hours at room temperature while shaking at 500 rpm on a Benchmark Orbishaker™ ...
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bioRxiv - Immunology 2021Quote: ... Onestep ultra TMB 32048 ELISA substrate (Thermo Fisher Scientific) was added and incubated until color change was visible and the reaction was stopped by addition of 100 µl of 1M H2SO4 ...
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bioRxiv - Neuroscience 2020Quote: ... Total Aβ42 was measured via ELISA (Thermo Fisher, KHB3544), total apoE was quantified via ELISA (Abcam ab108813) ...
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bioRxiv - Neuroscience 2020Quote: ... using ELISA assays (both Invitrogen, KHB0041 and KHO0631, respectively) according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... ELISAs were developed with ABTS solution (Thermo Fisher 002024) and absorbance was measured at 415 nm and 695 nm ...
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bioRxiv - Molecular Biology 2020Quote: ... and SuperSignal ELISA Femto Maxiumum Sensitivity Substrate (Thermo Scientific). Relative Light Units (RLU ...
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bioRxiv - Immunology 2020Quote: ... and IL-18 ELISA (Thermo Fisher Scientific, BMS618-3) according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... TNFα was quantified by ELISA (Invitrogen, 88-7346-88) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasma concentrations of corticosterone were measured using ELISA (ThermoFisher), per manufacturer specifications ...
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bioRxiv - Cell Biology 2024Quote: ... human IL-6 Uncoated ELISA (88-7066-88, Invitrogen) and human GRO alpha (CXCL1 ...
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bioRxiv - Neuroscience 2023Quote: were measured in cell culture supernatant using ELISA (Invitrogen). Absorbance was read using a Thermo Scientific MultiSkan Go at 450nm.
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bioRxiv - Cancer Biology 2022Quote: ... ELISA assays used were AREG (EMAREG; Thermo Fisher Scientific).
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bioRxiv - Immunology 2023Quote: ... Onestep ultra TMB 32048 ELISA substrate (Thermo Fisher Scientific) was added to each well and incubated until color change was visible before stopping the reaction with 100 µl of 1 M H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... and incubation with ELISA Pico Chemiluminescent Substrate (ThermoFisher Scientific). Chemiluminescence was read on an EnVision 2105 Multimode Plate Reader (Perkin Elmer) ...
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bioRxiv - Microbiology 2024Quote: ... followed by ELISA Stop Solution (ThermoFisher Scientific, MA, USA). The plates were read using BioTek 800TS absorbance reader at 450 nm (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... α- Hemoglobin was quantified by ELISA (ThermoFisher Scientific, Waltham, MA). Total glutathione peroxidase activities were measured by enzymatic assay (Cayman Chemical ...
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bioRxiv - Microbiology 2024Quote: ... We utilized the mouse IL-17a (homodimer) ELISA (ThermoFisher) according to the kit instructions ...
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bioRxiv - Immunology 2024Quote: ... Step four: 10uL of ELISA Pico Chemiluminescent Substrate (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... Tissue was processed and EdU was detected in whole mount using Click-iT EdU imaging kit (Life Technologies, catalog no. C10338 or C10340). Images were acquired by confocal microscopy and analyzed using Volocity 6 software.
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bioRxiv - Cancer Biology 2020Quote: ... was used for whole transcriptome library construction according to the Ion Total RNA-Seq Kit for the AB Library Builder™ System (Life Technologies, 4482416) protocol for barcoded libraries ...
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bioRxiv - Cancer Biology 2020Quote: ... prior to preparing single stranded complimentary DNA (sscDNA) from 200ng of total RNA (Ambion WT Expression Kit for Affymetrix GeneChip Whole Transcript WT Expression Arrays; Applied Biosystems, Carlsbad, CA) according to the Affymetrix User Manual (Affymetrix ...
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bioRxiv - Molecular Biology 2023Quote: ... The whole cell lysate were obtained with RIPA and the protein concentration was measured using BCA protein assay kit (23225, Thermo Scientific, MA, USA). Monoclonal antibody anti-DNMT1 (ab188453 ...
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bioRxiv - Plant Biology 2023Quote: ... and whole genomic DNAs were quantified using the Qubit dsDNA BR Assay Kit and Qubit 2.0 Fluorometer (Life Technologies, Carlsbad, CA, United States). Samples with high-molecular-weight DNA (>15 kb ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentration of the membrane fractions and whole cell lysates were measured using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). Myc-tag immunoprecipitation (IP ...
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bioRxiv - Genetics 2024Quote: ... 1:10 dilution of whole cell lysates in RIPA were used to quantify protein content using Pierce BCA Assay Kit (Thermo Fisher Scientific #23227). 20 μg protein was loaded per sample into 15-well Novex WedgeWell Tris-Glycine Mini gels (Invitrogen #XP08165BOX) ...
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bioRxiv - Zoology 2023Quote: ... Reverse transcription using 300 ng of whole purified RNA and oligo dT primers followed manufacturer instructions and reaction times for the SuperScript IV kit (ThermoFisher, Waltham, MA, USA). A no reverse transcriptase reaction was included as a control.
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bioRxiv - Systems Biology 2023Quote: Total RNA was extracted from whole blood samples using the MagMAX for Stabilized Blood Tubes RNA Isolation Kit (Thermo Fisher Scientific, Waltham, MA), respectively ...
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bioRxiv - Molecular Biology 2023Quote: ... Constructed clones were validated by a whole plasmid sequencing were used as a template to generate infectious RNA by using in vitro transcription (ThermoFisher SP6 mMessage kit) after a linearization with Not I enzyme ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration from whole cell lysate was measured by BCA assay using Pierce™ BCA Protein Assay Kit (Cat# 23227, Thermo Scientific™) as per manufacturer protocol and 40 µg of protein per well was separated on 12% SDS PAGE ...
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bioRxiv - Molecular Biology 2023Quote: ... 1:200 dilution of primary culture was added to sterile 96-well plate black/clear bottom plates (Thermo Scientific) in a final volume of 200 µL with 0.1 mM IPTG and antibiotics ...
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bioRxiv - Molecular Biology 2020Quote: ... and their whole brains were carefully dissected and placed individually in Eppendorf tubes containing 1 mL of TRIzol Reagent (Life Technologies). Total RNA was isolated using TRIzol Reagent according to the manufacturer’s recommendation and was subsequently purified on columns with Quick-RNA MiniPrep (Zymo Research ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes containing whole-cell lysates were also incubated with an HRP-conjugated rabbit anti-β-actin (Invitrogen MAB-32540, 1:1000). The Pierce ECL (Amersham ...
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bioRxiv - Neuroscience 2023Quote: ... Secondary antibodies used are: ECL anti-mouse immunoglobulin G (IgG) horseradish peroxidase linked whole antibody (1/10 000; Fisher Scientific; NXA931V) for anti-MAP1B ...
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bioRxiv - Cell Biology 2023Quote: Whole bone marrow from 1 femur and whole periosteum from 2 femurs were isolated and digested as previously described and plated in α-MEM (Gibco) containing 20% FBS (HyClone) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 488-conjugated donkey antibody (Invitrogen A21206), (2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 647-conjugated donkey antibody (Invitrogen A31573). Washed three times for 30 minutes each in TNTT at 4 ℃ to remove secondary antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... the absorbance was evaluated in Varioskan™ LUX Multi-function microplate reader (Thermo Scientific, USA) at a wavelength of 490 nm ...
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bioRxiv - Genomics 2021Quote: ... After Permeabilization multi-stain solution (10 units of Alexa Fluor™ 568 Phalloidin (ThermoFisher #A12380), 0.01mg/ml Hoechst 33342 (Invitrogen #H3570) ...
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bioRxiv - Bioengineering 2019Quote: ... at 100 rpm or end-over-end mixer (Multi-Purpose Tube Rotator 88861049, Fisher Scientific) at 15 rpm for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were reverse transcribed with random hexamer primers using Multi-Scribe Reverse Transcriptase (Life Technologies). Real-time reverse transcription PCR was done with RPL27 as housekeeping gene ...