Labshake search
Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Prostaglandin E2 PGE2 Multi Format ELISA Kit 1 Whole Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2019Quote: ... 1 mM DTT using Zeba spin desalting plates (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... 500 ng of each Acr vector and GFP plasmid used as a transfection control were transfected in a 6-well format using Lipofectamine 2000 (Invitrogen). The total DNA amount was kept constant by adding a stuffer plasmid in all cases.
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bioRxiv - Microbiology 2021Quote: Total RNA from 2×105 (24-well format) MDMs was isolated at one day post-infection using TRIzol reagent (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: Stably transfected derivatives of HEK293 FLP-In T-Rex cells that expressed WT DIS3 or the catatonically inactive version (G766R) of DIS3 were cultured for transfection in six-well format in DMEM (Gibco) supplemented with 10% FBS (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... Raw data files were converted to mascot generic file format using Proteome Discoverer 1.4 (Thermo Fisher Scientific, San Jose, CA). The MS/MS spectra were subjected to Mascot database search against UniProt mouse protein database ...
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bioRxiv - Neuroscience 2020Quote: ... The chips were washed and stained by the Affymetrix Fluidics Station using the standard format and protocols as described by Affymetrix. The probe arrays were stained with streptavidin phycoerythrin solution (Molecular Probes ...
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bioRxiv - Neuroscience 2022Quote: ... All assays were run in a pre-spotted 384-well format on a QuantStudio-12K-Flex system (Thermo Fisher Scientific). Samples were measured in triplicate ...
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bioRxiv - Molecular Biology 2020Quote: ... Amplifications were performed in technical triplicates in a 384-well format using the QuantStudio™ 12K Flex system (Life Technologies). Housekeeping genes (e.g ...
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bioRxiv - Molecular Biology 2020Quote: ... Raw MS/MS data were converted into an MGF format using Proteome Discoverer 1.2 (Thermo Fisher Scientific, Waltham, MA, USA). The exported MGF files were searched with Mascot v2.3.01 in a local server against the mouse SwissProt database ...
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bioRxiv - Cell Biology 2020Quote: ... The MS/MS data were converted to the mascot generic format with the Proteome Discoverer software (Thermo Scientific, ver. 1.1). The files were processed with the MASCOT algorithm (version 2.2.1. ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from rSARS-CoV-2/mCherry-Nluc-infected (MOI 0.01) Vero E6 cells (1.2 x 106 cells/well, 6-well format) using TRIzol reagent (Thermo Fisher Scientific) based on the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: RNAi in HeLa cells was performed in a 24-well format with 2.5 pmol of small interfering RNAs final concentration (siRNAs) using Lipofectamine® RNAiMAX Transfection Reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1×103 cells were seeded per well in either a 48-well or 8-chamber format in DMEM high glucose (Gibco) and refreshed every 3 to 4 days.
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bioRxiv - Cancer Biology 2019Quote: ... Clonal selected U2OS cells stably expressing MYCT58A under the control of the retroviral LTR were transfected with 50nM siRNA in a 96-well format using Dharmafect1 (Dharmacon) or Lipofectamine RNAiMAX (Invitrogen). 24 hours after transfection ...
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bioRxiv - Plant Biology 2019Quote: ... as well as custom pENpAtPAD4 StrepII-YFP (Supplemental Dataset 8 with sequence in.gbk format) was performed using LR Clonase II (11791100, Life technologies).
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bioRxiv - Developmental Biology 2022Quote: ... qPCR was performed in a 96-well format using the SYBR™ Select Master Mix for CFX (Thermo Fisher Scientific) and a Biorad CFX96™ Real-Time System ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were transfected for 48 hours with 25 pmol per well (24-well format) of miRNA mimic Pre-miR miRNA precursors (Ambion, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... and hsa-miR-485-5p (catalog#17100, PM10837) or with 50 pmol per well (24-well format) of miRNA miRVana inhibitors (Ambion, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The raw data files were converted to Mascot generic format (mgf) files by Proteome discoverer (version 1.3, Thermo Fisher Scientific) and were submitted to peptide identification using a MASCOT search engine (version 2.5.1 ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen). After 6-8h transfection ...
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bioRxiv - Microbiology 2023Quote: ... Raw data files were processed and converted to mascot generic file (mgf) format using Proteome Discoverer 1.4 (Thermo Fisher Scientific). The mgf files were then used for protein sequence database search using Mascot algorithm to identify proteins ...
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bioRxiv - Neuroscience 2023Quote: ... fusion protein was transfected in human embryonic kidney (HEK293) cells in a 6-well format with or without full-length wild-type BACE1 using Lipofectamine 2000 (Invitrogen) as described previously (31) ...
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bioRxiv - Neuroscience 2022Quote: ... Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach; Floor Plate Progenitor Kit expansion protocol (ThermoFisher; A3165801) or terminally differentiated to DA neurons with all prior factors except in neurobasal base medium after day 25.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 were measured according to the manufacturer’s instructions in plasma and peritoneal lavage supernatant using Novex ELISA Kits (Thermofisher Scientific Ltd.).
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Neuroscience 2019Quote: Aß1-40 and Aß1-42 concentrations from cleared supernatants of differentiated iPSCs were quantified by using the Amyloid beta 40/ 42 Human ELISA Kits (#KHB3441 and KHB3481; Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... IL-23 and tumor necrosis factor alpha (TNF-α) were quantified in lung tissue lysates and BAL using commercial ELISA kits (Invitrogen, San Diego ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... to improve sensitivity and loading in ELISA or BLItz binding assays (EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit, ThermoFisher 21435).
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bioRxiv - Cell Biology 2021Quote: The cellular amounts of cAMP were quantified by using the non-acetylated cyclic AMP competitive ELISA Kit (Thermo Scientific, Cat. #EMSCAMPL) following to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of IL-2 in 2x diluted supernatants was measured using an IL-2 mouse ELISA kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... IL-6 and TNF-α in RAW264.7 cells culture supernatants were analyzed using ELISA kits (No. EMIL6RA, 88-7324-22, bms6002; Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... of hiPSC-aCMs and -vCMs were measured by a competitive enzyme-linked immunosorbent assay (ELISA) using a commercially available kit (Invitrogen, CA). The assay was conducted according to the manufacturer’s protocol and was measured using a spectrophotometric plate reader.
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bioRxiv - Cell Biology 2020Quote: ... The concentration of cAMP in the lysates was then measured by using a competitive ELISA kit for cAMP (Invitrogen, ref. EMSCAMPL) following the recommendations of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... The cells were incubated overnight and IFNγ levels in the supernatant were detected using the human IFN-γ ELISA Ready-SET-Go! kit (Invitrogen) accorded to manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: ... TNF-α was measured using a commercially available equine TNF-α ELISA reagent kit (ESS0017; Thermo Fisher Scientific, Frederick, MD, USA), with anti-equine TNF-α coating antibody and biotinylated anti-equine TNF-α detection antibody both diluted 1:100 ...
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bioRxiv - Physiology 2023Quote: ... Insulin concentrations were determined using a commercially available direct insulin enzyme-linked immunosorbent assay (ELISA) kit (#KAQ1251; Thermo Fisher Scientific, UK). Insulin sensitivity index (ISI ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... The quantification of Aβ40 and Aβ42 peptides present in the cell culture medium was performed utilizing the Amyloid beta 40 Human ELISA Kit (Thermo Fisher Scientific) and the Amyloid beta 42 Human ELISA Kit ...
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bioRxiv - Immunology 2024Quote: ... The amount of TNF-α and IL-6 in culture supernatants was quantified using commercial mouse ELISA kits (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... The IL-6 levels in plasma were analyzed using a Mouse IL-6 Uncoated ELISA kit (#88-7064, Invitrogen, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... The Alexa-647 labelling for detection used in SMC and in ELISA assays was performed using the Alexa Fluor-647 Monoclonal Antibody Labeling Kit from Thermo Fisher Scientific (catalog #A20186 ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then measured by using human Aβ1-42 enzyme-linked immunoassay (ELISA) kit (Catalog No. KHB3441, Invitrogen by Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: EV-depleted media/ACSF samples (50 µL volume) were used for measurement of Tau concentration by a mouse-specific total Tau ELISA kit (KMB7011, Thermo Scientific) or pS199 Tau ELISA kit (KMB7041 ...
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bioRxiv - Immunology 2023Quote: ... IL-1β and IL-18 levels were assayed in supernatant by duplicate using commercial ELISA kits (Thermo Fisher Scientific, MA, USA).
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bioRxiv - Microbiology 2023Quote: ... IL6 and IFNγ in sera were quantified using sandwich ELISA (83) according to manufacturer’s instructions using the respective cytokine estimation kits (ThermoFisher Scientific, USA). 3,3’,5,5’-Tetramethylbenzidine (TMB ...
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bioRxiv - Immunology 2023Quote: ... as well as between stratified patient plasma samples (n=7) over three time points using an IL-23 Human ELISA Kit (BMS2023-3, ThermoFisher, Canada), as per manufacturer’s protocol ...
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bioRxiv - Systems Biology 2023Quote: ... IgG1 and IgG4 in the basolateral media were quantified using human ELISA) IgG1 and IgG4 quantification kits (Invitrogen, EHIGG1 and BMS2095) according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples that were determined to be below the detection limit were quantified using <0.3 pg/mL sensitivity porcine TNF-α ELISA kit (Invitrogen, USA).