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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Staining solutions were HEPES-Ca-BSA-T with SYTO BC (1:1000, Thermo Fisher Scientific) and StrepMAB classic DY649-conjugate (1:150 ...
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bioRxiv - Immunology 2023Quote: ... at a final concentration of 1 µg/mL using Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Neuroscience 2023Quote: ... or GAD65/67 (1:500, Cat. No. PA5-104543, anti-rabbit, Invitrogen, Carlsbad, CA, USA), or MAP2 (1:500 ...
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bioRxiv - Physiology 2024Quote: ... 1 μg of RNA was used to generate cDNA (Superscript III; Invitrogen, Carlsbad, CA, USA). Oligo dT primers and primers for real-time PCR were obtained from Integrated DNA Technologies (Coralville ...
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bioRxiv - Immunology 2024Quote: ... Th17 consisted of 1 µg/mL anti-murine IFNγ (Cat# 16-7312 Invitrogen Carlsbad, CA), anti-murine IL-4 (Cat# 16-7041 Invitrogen Carlsbad ...
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bioRxiv - Physiology 2024Quote: ... the sections were incubated with AlexaFluor 488-conjugated secondary antibodies (1:200, Invitrogen, CA, USA), followed by nuclear staining with DAPI (Sigma Chemical Co.) ...
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bioRxiv - Physiology 2024Quote: ... following an isotype-specific secondary antibody conjugated with Alexa 488 (1:200, Molecular Probes, CA) binding ...
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bioRxiv - Immunology 2024Quote: ... or goat anti-rabbit AlexaFluor 594 secondary antibody (1:1000, Invitrogen #A-11012, Carlsbad, CA)) diluted in 0.1% Triton X-100 in PBS and then mounted with Vectashield Mounting Medium containing 4′,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Developmental Biology 2024Quote: ... Slides were then blocked for 1 hour in Cas Block (Thermo Fisher, cat number 008120). Primary antibody incubations were done at 4°C overnight (for catalog numbers and dilutions ...
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bioRxiv - Developmental Biology 2024Quote: ... Detection of antibody binding was with the appropriate species-specific Biotin-SP-conjugated goat anti-antibodies (1:400) followed by AlexaFluor 555-conjugated Streptavidin (1:600) (Life Technologies, Carlsbad, CA; 1:400).
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bioRxiv - Bioengineering 2022Quote: ... 4 μl of 4 mM resazurin sodium salt (Acros Organics, Belgium), 0.002 U purified Castellaniella defragrans geraniol dehydrogenase ...
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bioRxiv - Neuroscience 2024Quote: The DIV 13-14 cells growing on coverslips were fixed using 4 % PFA freshly prepared from a 16 % stock (Thermo Fisher Scientific, dissolved 1:4 in 1xPBS) for 10 min RT ...
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bioRxiv - Neuroscience 2020Quote: ... (Invitrogen, Carlsbad, CA), supplemented with B27 (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... (Invitrogen, Carlsbad, CA).
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bioRxiv - Immunology 2020Quote: (Gibco, Carlsbad, CA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Genomics 2021Quote: ... CA-beads (Invitrogen) and 19% Polyethylene glycol PEG ...
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bioRxiv - Cell Biology 2024Quote: ... (Gibco, Carlsbad, CA) and 10 ng/mL EGF (Peprotech)86 ...
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bioRxiv - Cell Biology 2020Quote: Fluorescent microtubules were polymerized from 4 mg/ml porcine tubulin (80% unlabeled and 20% Alexa Fluor 647 NHS ester-labeled; Invitrogen, Thermo Fisher Scientific, Carlsbad, CA, USA) for 2 h at 37 °C in BRB80 (80 mM PIPES ...
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bioRxiv - Biochemistry 2024Quote: ... CAS 7778-77-0, CAT P386-500), and dibasic potassium phosphate (K2HPO4, CAS 7758-11-4, CAT P288-500) were purchased from Fisher Scientific (Hanover Park, IL, USA). Naphthalene-2,3-dicarboxaldehyde (NDA ...
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bioRxiv - Neuroscience 2024Quote: WT and Fmr1 KO mice (4 months-old) received a bilateral injection of CholeraToxin (β subunit) conjugated to Alexa Fluor™ 555 Conjugate (catalog number: C34776; Invitrogen™, Carlsbad, CA). Prior to the injections ...
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bioRxiv - Cancer Biology 2024Quote: ... Flow-through and IP fractions were collected and subjected to electrophoresis using NuPAGE™ 4-12% Bis-Tris or NuPAGE™ 3-8% Tris-Acetate precast gels (Invitrogen, Carlsbad, CA, USA). After migration ...
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bioRxiv - Cancer Biology 2020Quote: ... MA and were cultured in a 1:1 mixture of F-12 Nutrient Mixture and Dulbecco’s Modified Eagle Medium (DMEM) (GIBCO, Carlsbad, CA) and supplemented with 20% knockout serum replacer ...
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bioRxiv - Cancer Biology 2020Quote: Both HME and HRE cell lines were cultured in a 1:1 mixture of F-12 Nutrient Mixture and Dulbecco’s Modified Eagle Medium (DMEM) (GIBCO, Carlsbad, CA) and supplemented with 5% fetal bovine serum (GIBCO) ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 3 mL containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Cell Biology 2021Quote: ... rabbit polyclonal anti-CLC7 (1:4000; AP11863b, Abgent, San Diego, CA) or mouse monoclonal anti-GAPDH (1:10,000; AM4300, Thermo Fisher Scientific) and horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit secondary antibodies (1 ...
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bioRxiv - Microbiology 2020Quote: ... hACE2 receptor (hACE2 Recombinant Rabbit Monoclonal Antibody, 1:50, Thermo-Fisher, Cat#SN0754) and DAPI nuclear stain (1:20,000, Invitrogen, Carlsbad, CA). The primary antibodies were detected with goat anti-rabbit-AP developed with Permanent Red warp (mach3 Biocare Medical).
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bioRxiv - Microbiology 2021Quote: ... and monolayers were overlaid with 3ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Microbiology 2020Quote: ... and monolayers were overlaid with 3ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco. Carlsbad. CA) with 10% (vol/vol ...
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bioRxiv - Microbiology 2022Quote: ... 1 mL of culture was added to 1 mL of Trizol pre-warmed to 37°C (Life Technologies, Carlsbad, CA, USA) and incubated at room temperature for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and monolayers were overlaid with 3 ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Immunology 2024Quote: ... or LAMP-1/CD107a monoclonal antibody specific for the lysosomal-associated membrane protein 1 (LAMP-1) of the parasitophorous vacuole (rat IgG2a FITC, Invitrogen, CA). Revelation was performed with 1.5 μg/ml streptavidin conjugated to Texas Red (Molecular Probes ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were lysed in a 1:1 solution of 2× Tris-Glycine SDS Sample buffer (Invitrogen Life Technologies, Carlsbad, CA, USA) and Tissue Protein Extraction Reagent (Pierce ...
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bioRxiv - Microbiology 2023Quote: ... and monolayers were overlaid with 3 ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Physiology 2021Quote: ... were immunostained for insulin (guinea pig anti-insulin pig from Abcam, 1:1000, overnight, 4’C, secondary antibody; Alexa Fluor 647-conjugated anti-guinea pig from Invitrogen; 1:500, 1 h room temperature) and nuclei were stained with DAPI (Sigma-Aldrich ...
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bioRxiv - Genetics 2024Quote: ... were incubated overnight at 4°C followed by the secondary antibodies (anti-rabbit 488, A11034 Invitrogen, 1:400; anti-mouse 555, A31570, Invitrogen, 1:400; DAPI, Roth, 1:400). Finally ...
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bioRxiv - Cell Biology 2020Quote: ... Infected MEFs were maintained in induction medium composed of DMEM/High Glucose:Medium 199 (4:1, HyClone:Gibco), 10% FBS (Gemini) ...
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bioRxiv - Biophysics 2020Quote: KPL-4 cells were transfected with the GV gene constructs and 1 µg/ml puromycin (Invitrogen) was added to cell cultures to select only the transfected cells ...
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bioRxiv - Neuroscience 2021Quote: ... slices were incubated with 4’,6-diaminodino-2-phenylindole (DAPI, Life Technologies D-21490, 1:2000) for 15 min ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclear dye 4’,6-diamidino-2-phenylindole (DAPI) at 1 l ng/ml (Molecular Probes) was added to visualise all cells ...
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bioRxiv - Physiology 2020Quote: ... Cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; 1:10,000; ThermoFisher, Table S13). Confocal images were acquired using an inverted laser scanning confocal microscope (Nikon Eclipse C1) ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were generally run in 1 mm polyacrylamide 4-20 % Novex MES/SDS gels (Thermo Scientific) and using a Mini Gel Tank ...
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bioRxiv - Immunology 2021Quote: Freshly isolated thymocytes or virus-transduced thymocytes were incubated with 4 μM Indo-1-AM (Invitrogen) in TCM at 37°C for 40 min ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
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bioRxiv - Cancer Biology 2021Quote: ... Chromatin immunocomplexes were precipitated for 1 hour at 4°C with blocked Dynabeads Protein G (Invitrogen), subjected to multiple wash steps and the chromatin eluted in elution buffer (50 mM Tris-HCl [pH 8.0] ...
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bioRxiv - Immunology 2021Quote: ... Nuclei were counterstained with 1/2500 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, ThermoFisher Scientific, USA). The cover slips were fixed in fluorescence mounting medium (Fluoromount-G ...
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bioRxiv - Cancer Biology 2021Quote: ... with a ratio of 1 μg DNA and 4 μl transfection reagent diluted in OptiMEM (Gibco). Lipofectamine 2000 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were further ruptured using a syringe and centrifuged at 14,000g for 30 min at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... and 1 mM of the serine protease inhibitor 4-(2-aminoethyl) benzenesulfonyl fluoride hydrochloride (ThermoFisher Scientific). The samples were sonicated and centrifuged at 14,000g for 30 min at 4°C ...