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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for OF 1 CAS 919973 83 4 99% since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... plugs were washed 4 times in 1×Wash Buffer (BNG) and 5 times in 1× TE Buffer (ThermoFisher Scientific, Waltham, MA). Then ...
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bioRxiv - Immunology 2023Quote: NK cells were activated by co-culturing NK cells with irradiated K562 cells expressing membrane bound interleukin-21 and 4-1BBL at 1:1 ratio in RPMI (Gibco, Thermo Fisher) supplemented with 10% FCS (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once with PBS and a mixture of 1:1:2 (w/w) of pcDNA5-αSyn (4 μg): pOG44 Flp-recombinase expression vector (pOG44, Invitrogen #V600520):PEI in 2 mL of DMEM was added for 8 h ...
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bioRxiv - Biophysics 2024Quote: ... protein fractions were mixed 1: 1 with SDS loading buffer and loaded on a NuPageTM 4-12% Bis-Tris PAGE Gel (Invitrogen™) along with Novex™ Sharp Pre-stained Protein Standards (range from 3.5 to 260 kDa ...
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bioRxiv - Cancer Biology 2024Quote: ... these plasmids were transfected into HEK293T cells alongside psPAX2 and pMD2.G at a mass ratio of 1 pMD2.G : 3 psPAX2 : 4 pLKO.1 using Lipofectamine 2000 (Invitrogen 11668019), and media containing the lentivirus was concentrated with Lenti-X Concentrator (Takara Bio 631232 ...
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bioRxiv - Cell Biology 2024Quote: ... All organoid media were refreshed every 3-4 days and organoids were passaged 1:4-1:8 every week by either mechanical disruption or enzymatic digestion using TrypLE Express Enzyme (Gibco, 12604013). If organoids were passaged by enzymatic digestion into single cells ...
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bioRxiv - Neuroscience 2024Quote: ... and probed with the primary antibody overnight at 4°C (anti-Pyk2 [1:500; sc-1514, Santa Cruz Biotechnology] and anti-pPyk2 [1:500; 44-618G, Invitrogen]). After several washes with TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... and then washed in 0.1M PBS containing 0.1% tween before a 4 hr incubation in a half block solution containing 1:1000 goat anti-rabbit 488 (Invitrogen, A11008). Finally ...
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Malaria parasites undergo a rapid and extensive metamorphosis after invasion of the host erythrocytebioRxiv - Microbiology 2024Quote: Wildtype parasites and parasites lacking PV6 were allowed to invade erythrocytes for 4 hours in cRPMI and then washed and resuspended at 1% haematocrit in PBS containing 1% Albumax (Life Technologies). The retention rate was evaluated by microsphiltration (microsphere filtration ...
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bioRxiv - Biochemistry 2024Quote: ... Following centrifugation at 48,000 rcf for 1 hour at 4°C the supernatant was applied to 1 mL Ni-NTA agarose beads (ThermoFisher Scientific) and rocked for 1 hour at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... A total of 4 μg of protein were extracted from each lysate and reduced with 1:1 dilution of 2-mercaptoethanol (Thermo Scientific) and 2× Laemilli Buffer (Biorad) ...
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bioRxiv - Cell Biology 2024Quote: Ovaries from 8 to 15 females were dissected in PBSTw (PBS with 0.1% Tween-20) and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) in PBS and heptane (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2021Quote: ... Secondary antibodies: donkey anti- mouse Alexa Fluor 647 (1:10, ThermoFisher Scientific, Carlsbad, CA, USA) and donkey anti-rabbit Alexa Fluor 555 (1:10 ...
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bioRxiv - Genomics 2020Quote: ... (1) total RNA of 12 samples was extracted by TRIzol Reagent (Invitrogen, Carlsbad, CA, USA), and DNA contaminants were removed with RNase-free DNase I (TaKaRa ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then maintained in the appropriate primary antibodies – LNX1 1:200 (Invitrogen, Carlsbad, CA) or Numb 1:200 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... and the sorted cells were counterstained with 1 μg/mL Hoechst 33342 (Invitrogen, Carlsbad, CA), then washed and resuspended with 1% BSA/PBS for imaging or 16S sequencing.
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488-conjugated donkey anti rabbit (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA), Alexa Fluor 568-conjugated donkey anti rabbit (1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... at a dilution of 1:100 and goat anti-rabbit-Alexa 488 (Invitrogen, CA, USA) at a dilution of 1:500 ...
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bioRxiv - Biophysics 2020Quote: ... and 0.2 μm fluorescent beads at a dilution of 1:1000 (Molecular Probes, Carlsbad, CA). After adding a photo-initiator ...
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bioRxiv - Cell Biology 2021Quote: ... AlexaFluor™ secondary antibodies were used to visualize the samples (Invitrogen, Carlsbad, CA, 1:200) and DAPI (2µg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Tissues were homogenized and resuspended in 1 ml of TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and then we proceeded according to manufacturer’s instructions for RNA extraction ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used were as follows: mouse anti alpha-SMA (1:200, Invitrogen, Carlsbad, CA, USA); Myosin-IIB (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... or Alexa-488 conjugated goat anti-rabbit secondary antibody (1:500; Invitrogen, Carlsbad, CA, USA). The staining results were examined and photographed under a fluorescence microscope (BX63 ...
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bioRxiv - Neuroscience 2022Quote: ... and Alexa-594 conjugated goat anti-rabbit secondary antibody (1:500; Invitrogen, Carlsbad, CA, USA) or Alexa-488 conjugated goat anti-rabbit secondary antibody (1:500 ...
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bioRxiv - Molecular Biology 2021Quote: ... and goat-anti-mouse AlexaFluor 647 (1:2000, #A31571, Invitrogen/Life Technologies, Carlsbad, CA, USA) in blocking solution (0.5% BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... and goat-anti-mouse AlexaFluor 647 (1:2000, #A31571, Invitrogen/Life Technologies, Carlsbad, CA, USA) in blocking solution (0.5% BSA ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 568-conjugated donkey anti rabbit (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA)and Alexa Fluor 488-conjugated donkey anti mouse (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 488-conjugated donkey anti rabbit (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA), Alexa Fluor 568-conjugated donkey anti rabbit (1:2000 ...
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bioRxiv - Neuroscience 2020Quote: ... Alexa Fluor 568-conjugated donkey anti rabbit (1:2000, Life Technologies-Invitrogen, Carlsbad, CA, USA)and Alexa Fluor 488-conjugated donkey anti mouse (1:2000 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunoblots were developed using HRP-conjugated Goat anti-Rabbit (Life Technologies, Carlsbad, CA, 1:30,000) or Goat anti-Mouse (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... and TaqPath™ 1-Step RT-qPCR Master Mix (Applied Biosystems, Foster City, CA, USA). PCR cycler conditions were 2 min at 95 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... After blocking for 2h at RT in CAS-Block (1:10 in PBST; ThermoFisher, #008120) blocking reagent was replaced by antibody solution (diluted in CAS-Block ...
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bioRxiv - Immunology 2021Quote: ... the co-cultures were contra-stained with 1 µg/mL Hoechst 33342 (Invitrogen, Carlsbad, CA) and imaged using Nikon inverted microscope Eclipse Ti-E (Nikon Instruments ...
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bioRxiv - Genomics 2022Quote: ... and a 1-μl aliquot was quantified with a Qubit 3.0 Fluorometer (Invitrogen, Carlsbad, CA). The purified library was loaded onto flow cells for sequencing on a PromethION (Biomarker ...
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bioRxiv - Microbiology 2022Quote: ... 100 µL of asynchronous parasites were incubated with 1 µg/mL DAPI (Invitrogen, CA, USA) and 30 nM MitoTracker CMX-Ros (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) was obtained from Life Technologies (Carlsbad, CA) and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Immunology 2022Quote: ... A 96-well plate was coated with anti-CD3e (1 mg/mL; Thermofisher, CA, USA) and anti-CD28 (5 mg/mL ...
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FGF21 has a sex-specific role in calorie-restriction-induced beiging of white adipose tissue in micebioRxiv - Physiology 2022Quote: ... 1 μg of RNA was used to generate cDNA (Superscript III; Invitrogen, Carlsbad, CA, USA). Oligo dT primers and primers for real-time PCR were obtained from Integrated DNA Technologies (IDT ...
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bioRxiv - Physiology 2022Quote: ... 1 μg of RNA was used to generate cDNA (Superscript III; Invitrogen, Carlsbad, CA, USA). Oligo dT primers and primers for real-time PCR were obtained from Integrated DNA Technologies (IDT ...
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bioRxiv - Systems Biology 2024Quote: ... frozen cell pellets were resuspended in 1 ml of Trizol reagent (15596018; Invitrogen, Carlsbad, CA) and total RNA was purified following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted in 1 ml of TRI Reagent™ Solution (Ambion, Invitrogen; Carlsbad, CA) using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted in 1 ml of TRI Reagent™ Solution (Ambion, Invitrogen; Carlsbad, CA) using bead-beating for 30 min at RT followed by purification using Direct-zol RNA Miniprep kit (Zymo Research ...
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bioRxiv - Immunology 2024Quote: ... and goat anti-mouse IgG antibody conjugated with AlexaFluor 647 (1:1000; Invitrogen, Carlsbad, CA) in blocking buffer were added and incubated for 2 hours in the dark ...
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bioRxiv - Microbiology 2024Quote: ... The cell pellets were immediately suspended in 1 mL TRIzol reagent (Invitrogen, Carlsbad, CA, USA), vortexed thoroughly ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were incubated with Alexa Fluor-conjugated secondary antibody (1:200; Invitrogen, Carlsbad, CA) and/or Alexa Fluor 594-conjugated phalloidin (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... the RPE single cell suspension was stained with 1 μl SYTO64 dye (Invitrogen, Carlsbad, CA) in 1mL PBS for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were incubated in rabbit anti-GFP antibody (1:1000, A111222, Life Technologies, Carlsbad, CA) in PBS at RT for 24 hours ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stained with Hoechst-33342 (diluted 1:10,000 in PBS; Invitrogen, Carlsbad, CA, USA). Z-stack images with an interval of 5 µm and an overall z-layer of 500 µm were acquired using a Lionheart FX automated microscope with a 10× objective (BioTek ...
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bioRxiv - Cancer Biology 2023Quote: ... and cDNA was generated from 1 μg of RNA with SuperscriptII (Invitrogen, Carlsbad, CA, USA). PCR primers (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... Staining solutions were HEPES-Ca-BSA-T with SYTO BC (1:1000, Thermo Fisher Scientific) and StrepMAB classic DY649-conjugate (1:250 ...