Labshake search
Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 6 Chloro 9 3 N 2 chloroethyl ethylamino propylamino 2 methoxyacridine dihydrochloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... After 30 min incubation with 100 ng/mL 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific D1306), specimens were washed three times with PBS−/− ...
-
bioRxiv - Neuroscience 2021Quote: ... Isolated MEFs were plated in 2 wells of a 6-well plate and cultured overnight in DMEM (Gibco) supplemented with 10% FBS ...
-
bioRxiv - Neuroscience 2021Quote: ... Cells fixed in methanol were incubated with 4’,6-Diamidino-2-pheny-lindoldihydrochloride (DAPI; Thermo Fisher Scientific #D1306) for 10 minutes and subsequently imaged at 6 locations per condition using a Leica DMi8 microscope ...
-
bioRxiv - Cell Biology 2021Quote: ... Slides were mounted using ProLong™ Gold Antifade Mountant with 4’,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific). Imaging was performed using a Leica TCS SP8 confocal microscope.
-
bioRxiv - Neuroscience 2021Quote: ... incubated for 10 min in PBS/10 ug/mL 4’-6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) to stain nuclei ...
-
bioRxiv - Neuroscience 2022Quote: Immunofluorescence was performed using glial fibrillary acidic protein (GFAP) and 4′,6-diamidino-2-phenylindole (DAPI) solution (ThermoFisher). GFAP primary and secondaries were diluted to a ratio of 1:1000 and 5:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
-
bioRxiv - Bioengineering 2022Quote: ... the cell nuclei were stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific #62248) in DPBS for 15 min at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... The slices were mounted with ProLong Diamond Antifade Mountant with 4’,6-diamino-2-phenylindole (DAPI) (ThermoFisher Scientific). Fluorescent images were analyzed with a Nikon confocal microscope (Nikon A1RMP ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... dishes were mounted with ProLong Diamond mounting medium containing 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Waltham, MA), and imaged using a Zeiss LSM 780 laser confocal microscope equipped with a C-Apochromat 40x/1.2W objective.
-
bioRxiv - Microbiology 2022Quote: C57BL/6 or db/db mice were ear-punched using a 2 mm Animal Ear-Punch (Fisher Scientific) and 1×108 CFU of bacteria topically applied to the entire mouse using sterile cotton swabs at Day 0 and Day 4 post ear-punch ...
-
bioRxiv - Neuroscience 2022Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog #D3571), washed again ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Slides were washed five times in PBST and counterstained with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D1306) and mounted in Aqua-polymount (Polysciences Ince. ...
-
bioRxiv - Microbiology 2024Quote: ... Then, 4′,6-diamidino-2-phenylindole (DAPI, Solabio) and Alexa Fluor 488 Conjugate Concanavalin-A (Invitrogen, CA, USA) were added to each slide and incubated for 5 min at room temperature ...
-
STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
-
bioRxiv - Microbiology 2023Quote: ... and counterstained with 4′,6-diamidin-2-phenylindole (DAPI, 4 μg mL-1, Thermo Fisher Scientific, MA, USA). From the 18 biofilms from each year ...
-
bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected as follows: 2 μg eGFP-SHLD3 constructs and 6 μl Lipofectamine 2000 (Thermo Scientific) were incubated in Opti-MEM for 20 min at room temperature before being added to the cells whose media had been changed to McCoy’s with 10% FBS and no antibiotics ...
-
bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained by incubation with 0.5 – 1 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, D1306, Invitrogen) in PBS for 10 min at RT ...
-
bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
-
bioRxiv - Zoology 2023Quote: ... The samples were stained by the 4’,6-diamidino-2-phenylindole (DAPI) and the rhodamine-conjugated phalloidin (ThermoFisher). The signals were detected by fluorescent microscopy (Nicon ...
-
bioRxiv - Bioengineering 2023Quote: ... The cells were washed 4 times with PBST and counterstained with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) at 1:1000 dilution ...
-
bioRxiv - Bioengineering 2023Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
-
bioRxiv - Immunology 2023Quote: ... Slides were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) and mounted with ProLong Diamond Antifade Mountant (ThermoFisher). Images were acquired using a 20X objective lens Zeiss LSM 880 Confocal Laser Scanning Microscope.
-
bioRxiv - Immunology 2023Quote: ... RD114 cells were seeded at 2 × 105 cells/well in a 6-well plate with DMEM (Gibco, USA) supplemented with 10% FCS (Sigma-Aldrich ...
-
bioRxiv - Biophysics 2023Quote: ... Sample were rinsed in PBS and incubated with 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Scientific™, USA) for 5 mins and Alexa 594-phalloidin (Invitrogen™ ...
-
bioRxiv - Synthetic Biology 2023Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/μL) ...
-
bioRxiv - Physiology 2023Quote: ... slides were stained with 1:10,000 DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific; Cat. No.: D3571) for 10 min at room temperature before coverslips were applied with PBS and glycerol (1:1 ...
-
bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
-
bioRxiv - Developmental Biology 2023Quote: ... ATs and TTs were fixed in 4% PFA and stained with 4′,6- diamidino-2-phenylindole (DAPI) (Invitrogen) to identify cell nuclei ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells were washed in FACS buffer and then stained with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher) or 7AAD (Biolegend ...
-
bioRxiv - Microbiology 2024Quote: ... the cell nucleus was stained with 1:1000 DAPI (4’,6’-diamidino-2-phenylindole; Invitrogen; Catalog No: D1306) nuclear stain for 10 minutes at RT ...
-
bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
-
bioRxiv - Cell Biology 2022Quote: ... 6 or 9 μl of 20 μM siRNA and 4 μl of lipofectamine RNAi MAX (Invitrogen, 13778150) were suspended in 100 μl of water (Sigma ...
-
bioRxiv - Microbiology 2020Quote: ... 10 ml l1 glycerol and 20 g l−1 Bacto agar) supplemented with 25 µg ml−1 5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal, Thermo Fisher Scientific). Overnight cultures of the control strains were normalized to OD600 = 1.0 and inoculated as 20 µl spots on the agar plates containing the biosensor ...
-
bioRxiv - Genetics 2021Quote: ... RNA was extracted from control and CRISPR-Cas9 targeted Calu-3 cells (N = 3 biological replicates, with 3 technical replicates per experiment per condition) and prepared using Trizol Reagent (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2020Quote: ... the sections were sequentially incubated with mouse polyclonal antibody to SARS-CoV-2 N protein (1:500 dilution) and HRP-conjugated goat anti-mouse IgG secondary antibody (1:5000 dilution, Invitrogen). The sections were observed under microscope (Olympus ...
-
bioRxiv - Molecular Biology 2020Quote: ... at 200 V for 40 min in NuPAGE 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (cat. no. NP0002, Invitrogen), supplemented with NuPAGE antioxidant (cat ...
-
bioRxiv - Immunology 2021Quote: ... virus-infected cells were detected using an in-house rabbit polyclonal antibody specific for SARS-CoV-2 N/M/E followed by horseradish peroxidase-conjugated goat anti-mouse IgG (Invitrogen). TCID50 was calculated using the Reed-Muench method.
-
bioRxiv - Synthetic Biology 2021Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
-
bioRxiv - Microbiology 2020Quote: ... The plasmids were purified from 2 ml of the O/N culture using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), and sent for Sanger sequencing to DNA Sequencing and Genomics ...
-
bioRxiv - Microbiology 2022Quote: ... The blocking buffer was then aspirated and 50 μL of a 1:4000 dilution of rabbit anti-SARS-CoV-2 nucleocapsid (N) antibody (MA536086, Invitrogen) in blocking buffer was added and incubated for 2 h at 37°C ...
-
bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
-
bioRxiv - Microbiology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
-
bioRxiv - Immunology 2021Quote: ... IF staining was performed on deparaffinized and rehydrated tissue sections using rabbit anti-SARS-CoV-2-N protein antibody together with AF568-conjugated goat anti-rabbit IgG (Invitrogen). Briefly ...
-
bioRxiv - Bioengineering 2021Quote: ... The ovaries were dissected into 2–4 pieces and transferred into maintenance media (α-MEM, P/N 32561, Gibco, USA) and placed in a CO2 incubator prior to encapsulation.
-
bioRxiv - Microbiology 2019Quote: ... prepared minipreps from 2 ml of O/N cultivation at 37 °C using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific), checked for the presence of insert using restriction digest and agarose gel electrophoresis ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting PCR product (N linker::CeTurboID::c-Myc::mNeongreen::3XFLAG) was cloned into position 2 of a Multisite Gateway pDONR vector (ThermoFisher).
-
bioRxiv - Immunology 2021Quote: ... The SARS-CoV-2 N standards were generated by in-vitro transcription of the generated SARS-CoV-2 N cDNA using the MegaScript T7 Transcription kit (Invitrogen), followed by using the MEGAclear Transcription Clean-Up Kit ...
-
bioRxiv - Immunology 2021Quote: Codon-optimized sequences for the N or the spike S protein of SARS-CoV-2 were cloned into the pVAX1 expression plasmid (ThermoFisher) optimized for DNA vaccinations referred to as pVAX1-SARS2-N and pVAX1-SARS2-S 81 ...