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Citations for Thermo Fisher :
1601 - 1650 of 10000+ citations for 6 Chloro 7 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... anti-Thy1.1 (1:500, OX-7; Invitrogen) anti-CD4 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... The QuantStudio 7 Flex cycler (Thermo Fisher) was used for amplification ...
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bioRxiv - Microbiology 2023Quote: ... with the Quantstudio 7 Flex (Life Technologies) to quantitate relative levels of transcriptional activation or viral RNA compared to the house keeping gene HPRT1 ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were stained with 7-AAD (Thermofisher), and live cells were collected using BD FACSMelody™ Cell Sorter (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... then with 7-AAD (Invitrogen; cat # A1310). The cells were sorted into live (7-AAD- ...
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bioRxiv - Cancer Biology 2023Quote: ... and the QuantStudio 7 System (Applied Biosystems). The following primer/probes were used ...
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bioRxiv - Microbiology 2023Quote: ... GL7 (clone GL-7, Thermo Fisher Scientific), CD21/35 (clone eBio4E3 ...
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bioRxiv - Cancer Biology 2023Quote: ... 7-AAD (Invitrogen, Carlsbad, CA; 1:1000) was used to determine dead cells ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.2 with 7% SDS (Thermo Fisher) for 1 h at 45 °C ...
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bioRxiv - Microbiology 2023Quote: ... and a QuantStudio 7 Flex (Life Technologies) or a 7500 Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genomics 2024Quote: ... and analyzed on QuantStudio 7 (Applied Biosystems) normalized to TBP/HPRT1 Taqman probe (Supplementary Table 6).
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bioRxiv - Genetics 2024Quote: ... and 7 mL nuclease-free water (Invitrogen). 1 µL of 1:40 diluted fluorescent probes were added and the nematodes incubated overnight at 30°C in the dark ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... anti-Claudin-7 (rabbit, Invitrogen #34-9100), 1:100 ...
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Loss of intermicrovillar adhesion impairs basolateral junctional complexes in transporting epitheliabioRxiv - Cell Biology 2024Quote: ... anti-Claudin-7 (rabbit, Invitrogen #34-9100), 1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... for 1h at room temperature before rinsing and incubation with streptavidin-HRP (SNN1004, Invitrogen, 1:5000) 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... After incubation for 1h with the appropriate secondary antibodies (Alexa Fluor 488 or 594 (Thermo Fisher)) ...
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bioRxiv - Molecular Biology 2020Quote: ... and incubated for 1h on ice with or without RNase (10μg/mL RNase A) (Thermo Fisher). Lysate was centrifuged at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: Cells were harvested via centrifugation at 21,000xg 4°C 1h (Thermo scientific, Sorvall Lynx 4000 centrifuge), the supernatant discarded and the resulting pellet resuspended in 1ml of spent medium ...
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bioRxiv - Cell Biology 2022Quote: ... followed by PBS washes (3x) and staining with Hoechst (Life Technologies, 1h, 1:10000 in PBS). Cells were imaged using a 20x water immersion objective on a high content confocal microscope (Molecular Devices™ ImageXpress Micro XL).
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bioRxiv - Synthetic Biology 2019Quote: ... cells were incubated with a goat anti-rabbit secondary antibody (Alexa 633, Invitrogen; 1:100, 1h) and then analysed by a Fluorescence Activated Cell Sorter (Aria III ...
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bioRxiv - Physiology 2021Quote: ... The reactions were then incubated for 1h at 4°C with protein A/G dynabeads (Invitrogen) pre-blocked in PBS/0.5% BSA/0.5% Tween ...
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bioRxiv - Molecular Biology 2021Quote: ... for 1h followed by incubation with goat α-rabbit Alexa Flour 488 (Thermo Fisher, A-11008) for 1h ...
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bioRxiv - Cell Biology 2022Quote: ... and goat anti-mouse Alexa Fluor 488 secondary antibody (1:500, 1h; A-21121, Invitrogen, Germany) were used ...
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bioRxiv - Genomics 2022Quote: ... primary incubation was performed for 1h (anti-fibrillarin monoclonal mouse, 1:100, MA3-16771, Thermo Scientific), followed by incubation with secondary antibody (anti-mouse monoclonal FITC ...
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bioRxiv - Developmental Biology 2023Quote: ... after 1h of secondary antibody incubation fluorochrome conjugated phalloidin (Alexa Fluor™ 546 Phalloidin, Invitrogen™) was added to the secondary antibody solution for F-actin membrane staining for another hour ...
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bioRxiv - Cancer Biology 2023Quote: ... Each coverslip was then incubated for 1h at 37ºC with Duolink PLA probes (Thermo Fisher Scientific) diluted in blocking solution ...
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bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
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bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
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bioRxiv - Immunology 2024Quote: ... overnight at 4°C and the relevant secondary for 1h at room temperature (Streptavidin-AF488 Invitrogen S11223 at 2 µg/mL final ...
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bioRxiv - Molecular Biology 2021Quote: ... The probes were then washed and the cells/slides incubated with secondary antibodies (goat anti-rabbit Alexa fluoro 488, Invitrogen, 1:200 dilution) for 1hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were stained according to the same protocol as adult spinal cords using anti-2H3 (1:300, DHSB AB2314897) and Alexa fluoro-568 anti-mouse (1:250, Thermo Scientific, A-21124). Images were taken using a 20x lens on a Zeiss Axio Scan Z1 slide scanner ...
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bioRxiv - Physiology 2021Quote: ... and incubated with secondary antibody (conjugated goat anti-rat Alexa fluoro 546, 1:1,000 dilution, A11081; Invitrogen by ThermoFisher Scientific; Waltham, MA, USA) for 30 minutes ...
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bioRxiv - Bioengineering 2023Quote: ... The suspension was prepared by adding 40 μm diameter polystyrene beads (Fluoro-Max Green 35-7B, Thermo-Fisher) to 50 mL of 1X PBS (Gibco PBS, Fisher Scientific) to achieve a final concentration of approximately 30 beads/mL.
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bioRxiv - Neuroscience 2023Quote: ... followed by two final washing steps in PBS for 15 minutes each before they were mounted in Fluoro mount-G (Invitrogen, Waltham, MA, USA). Z-stacks were acquired using a Leica TCS SPE confocal microscope (Leica Microsystems ...
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bioRxiv - Biochemistry 2023Quote: Kinetic measurements of TrpBwt and its mutants were performed by monitoring 5-fluoro-L-Trp formation in a plate reader (Thermo Scientific Varioskan Lux) over 20 min at 290 nm using ΔE290 = 1.89 mM−1·cm−1 ...
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bioRxiv - Bioengineering 2020Quote: ... 3 and 7) were chosen for staining the actin filaments of the cultured cells using Alexa Fluor™ 488 phalloidin (Thermo Fisher Scientific, A12379, the Netherlands), and nuclei using 4,6-diamideino-2-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Interleukin 6 (IL-6) was measured using an IL-6 ELISA kit following manufacturer’s instruction (Invitrogen, Catalog # CHC1263). Similarly ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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bioRxiv - Synthetic Biology 2020Quote: ... cells from 2-D or 3-D cultures were harvested from one well of a 6-well plate and dissociated with Accutase (Thermo Fisher #A1110501), washed three times with 1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... total 2 μg was diluted into 6 μl of reagent (1:3 ratio) in the 100 μl of Opti-MEM I medium (31985062, Thermo Fisher Scientific) and incubated for 15 min at RT ...
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bioRxiv - Bioengineering 2022Quote: ... pools of 10 couples (n=8) were maintained up to 1 month in 6 well plates (9.6 cm2) using 3 mL of M199 (GIBCO, 41150-020, UK), DMEM (GIBCO ...
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bioRxiv - Immunology 2020Quote: ... were mixed by adding 3 μL of each (150 pmol) and 6 μL (180 pmol) of TruCut Cas9 Protein v2 (Thermo Fisher Scientific) and incubated for 10 minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... 1ml of bone marrow was incubated in one well of 6-well plate with 3 ml StemPro™ MSC serum-free medium (Gibco, Thermo Scientific). Media was changed after every two days until it reached to confluency ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher, A2644501). After 3 days from electroporation the cells were selected with StemFlex supplemented with G418 (25 µg/ml ...
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bioRxiv - Neuroscience 2021Quote: ... and replated at a density of 3*106 cells/well in Matrigel-coated 6-well plates containing NB media supplemented with BDNF (Thermo Fisher PHC7074), GDNF (Thermo Fisher PHC7044) ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Cell Biology 2021Quote: ... (Horizon Discovery Dharmacon, Lafayette, CO) in DharmaFECT 3 reagent (Horizon Discovery Dharmacon, T-2003) for 6-8 hours in OptiMEMm (Invitrogen, Waltham, MA)65 ...
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bioRxiv - Neuroscience 2020Quote: ... The sections were rinsed with PBS (3×10min) and incubated for 6 hours with secondary donkey anti-rabbit 488 antibodies (1:1000, ThermoFisher Scientific, A11006) and streptavidin 594 (1:750 ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Genomics 2020Quote: ... The cells were passaged every 2-3 days using 0.25% Trypsin-EDTA (1X) (Gibco, Cat# 25200056) as the dissociation buffer.