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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for 6 Chloro 7 fluoro 1H indole 2 3 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... plated at a density of 7 x 106 cells in a 175 cm2 tissue culture flask in 2% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were washed and re-suspended in PBS buffer free of KCl and incubated in the dark with 25-μM of 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) suspended in dimethylsulfoxide (DMSO) for 60 min at room temperature (Molecular Probes, Invitrogen). After incubation ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Library concentration was normalized to 2 nM and concentrations validated by qPCR on a ViiA-7 real-time thermocycler (Applied Biosystems), using qPCR primers recommended in Illumina’s qPCR protocol and Illumina’s PhiX control library used as a standard ...
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bioRxiv - Physiology 2023Quote: ... cells were incubated with fresh DMEM containing DDAO galactoside (9H-(1,3-dichloro-9,9-dimethylacridin-2-one-7-yl) β-D-Galactopyranoside) (Molecular Probes™) for 2 hours ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... fluorescence was observed under a confocal microscope (LSM880NLO, Zeiss, Germany) using 2’,7’-dichlorodihydro fluorescein diacetate (H2DCFDA, 10 InvM, Invitrogen, USA) as described by Yu et al ...
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bioRxiv - Plant Biology 2024Quote: ... Ten-μg protein of each sample were separated by electrophoresis in 15% SDS-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (Roth) for 7 min at 20V using the iBlot 2 Dry Blotting System (ThermoFisher Scientific), before blocking in TBS-T with 5% (w/v ...
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bioRxiv - Microbiology 2024Quote: ... 7 μl per injection was loaded onto a 2 mm by 0.3 mm Acclaim PepMap C18 trap column (Thermo Fisher Scientific) in 0.1% TFA at 15 μl/min before the trap being switched to elute at 0.25 μl/min through a 50 cm by 75 μm EASY-Spray C18 column ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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bioRxiv - Cell Biology 2021Quote: ... 6 EV samples (2 per cell type, 1 ZIKV and 1 control each) were fixed in 2% paraformaldehyde (PFA; Fisher Scientific, Cat #50-980-487) and processed as cited 59 ...
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bioRxiv - Neuroscience 2020Quote: ... Some slices (used for experiments in Fig. 2 and 3) were cultured in media that included 2 % B-27 Supplement (Thermo Fisher Scientific). Slices were maintained at 37°C and 5 % CO2 and used for experiments at DIV10-15.
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bioRxiv - Cell Biology 2022Quote: ... then incubated for 2-3 hours at room temperature with 2 µg/ml donkey anti-goat Alexa Fluor 488 (Cat# A21206, Thermo Fisher Scientific), 2 µg/ml donkey anti-rabbit Alexa Fluor 568 (Cat# A10037 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5 mM DTT, 50 mM HEPES, 3 mM MgCl2, 2 mM PMSF, 1 Pierce™ protease inhibitor mini tablet/2 L; Thermo Scientific). The lysate was sonicated then incubated with 500 units/1 L culture Benzonase Nuclease (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... followed by culturing with the neural induction medium supplemented with 20 ng/ml human FGF-2).61 The passage was performed twice per week (1:2 or 1:3) using Accutase (Cat #A1110501, Thermo Fisher Scientific) by vigorously breaking pellets to remove neuronal cells ...
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bioRxiv - Cancer Biology 2022Quote: ... for 1h at room temperature and mounted in ProLong Diamond Reagent with DAPI (Molecular Probes). Images were captured on a Zeiss LSM880 confocal microscope and analyzed with Fiji freeware.
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bioRxiv - Genomics 2019Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
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bioRxiv - Cancer Biology 2021Quote: ... imatinib or sunitinib for 1h or 8h were labelled with 25 µM HPG (Invitrogen, C10102) for the last 30 min of the drug treatment in L-methionine-depleted medium containing 5% dialyzed fetal calf serum (FCS ...
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bioRxiv - Cancer Biology 2020Quote: ... were incubated for 1h at room temperature and proteins visualized by chemiluminescence (Thermo Fisher Scientific). Each experiment was repeated at least four times.
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bioRxiv - Cell Biology 2020Quote: ... incubated 1h at room temperature with Alexa Fluor-conjugated secondary antibodies at 1:1,000 (ThermoFisher), washed 3 times in PBS and counterstained with DAPI at 1 μg.mL−1 in PBS before mounting.
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bioRxiv - Cell Biology 2020Quote: ... EDL muscles were dissected from tendon-to-tendon and incubated for 1h in DMEM (Gibco) containing 0.25% collagenase I (Worthington) ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 594-conjugated secondary antibodies was added for 1h at room temperature (Molecular Probes), and nuclei were counterstained using SlowFade Gold Antifade reagent (Vector ...
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bioRxiv - Microbiology 2021Quote: ... Supernatants were precleared 1h at room temperature with magnetic beads blocked with BSA (ThermoFisher Scientific) to avoid aspecific binding ...
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bioRxiv - Developmental Biology 2022Quote: ... and 10 % (vol/vol) Fetal Bovine Serum (Gibco; Heat-inactivated for 1h at 70°C). Stable cell lines were obtained by transfection with pMT puro vectors containing either Zasp52-PF or Zasp52-PF-ABM using Effectene (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (Invitrogen; SNN1008). Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... during 1h at room temperature before mounting in ProLong Gold Antifade reagent (P36941, Life Technologies). F-Actin was stained using Actistain (1:150 ...
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bioRxiv - Cell Biology 2023Quote: ... for 1h at 37°C and then with Proteinase K (Fisher Scientific, #25-530-049) for 2h at 55°C ...
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bioRxiv - Neuroscience 2023Quote: ... they were probed for 1h at RT with the fluorescent secondary antibodies (1:1000: ThermoFisher). Cell nuclei were stained with Hoechst 33342 (10 μg/mL ...
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bioRxiv - Genetics 2023Quote: ... transiently transfected HEK293T cells were starved for 1h in methionine-free RPMI (Thermo Fisher Scientific) and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer) ...
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bioRxiv - Genomics 2024Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
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bioRxiv - Microbiology 2023Quote: ... for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (SNN1008, Invitrogen). Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2021Quote: ... using a 10 min loading at 3 µL/min flow rate to a trap column (Acclaim™ PepMap™ 100, 2 cm × 75 µm, 3 µm, 100 Å - ThermoFisher Scientific). The separation was performed on an EASY-Spray™ C18 analytical column (25 cm × 75 µm ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... Mucociliary Transport (MCT) was assessed by automatically tracking the displacement of fluorescent microspheres (1 μm diameter, Fluoro Max Dyed Green, G0100, ThermoFisher, Waltham, MA) added to the surface of live ALI cultures by using the Fiji plugin TrackMate (38) ...
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bioRxiv - Biophysics 2019Quote: ... 0.2 μM Rhodamine B solution was used to align the confocal pinhole and 50 nm fluorescent polystyrene particles (Fluoro-Max Fluorescent Polymer Microspheres, 1% solid, Thermo Fisher Scientific) was prepared in de-ionized water as reference standard (D = 9.5 × 10−8 cm2/s in water ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated for 2–3 min at RT in trypsin (0.05% v/v; Gibco 25300-054), and tapped to release ...
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bioRxiv - Cancer Biology 2020Quote: ... and 12 mM 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium (MTT) (Thermo Fisher Scientific, #M6494). After 4 hours ...
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bioRxiv - Biochemistry 2020Quote: ... and SARS-CoV-2 [3]) was performed with Proteome Discoverer version 2.4 (Thermo Fisher Scientific). The search was restricted to human and SARS-COV-2 proteins ...
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bioRxiv - Cell Biology 2021Quote: ... 3 μg of RNA was digested with Turbo DNase (2 U/μl, # AM1907, ThermoFisher Scientific). 500 ng of DNase-digested RNA was tagged with G/I tails in a 10 μl reaction ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated for 3 days in presence of 100 U/mL IL-2 (Gibco) and 1 µg/mL PHA (Remel).
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bioRxiv - Microbiology 2020Quote: ... The modified nucleotide 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated by PCR using DreamTaq™ DNA Polymerase (Thermo Scientific™) ...
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bioRxiv - Microbiology 2021Quote: ... and 2-3 cm) after measuring the DNA concentration with Qubit (QubitⓇ 2.0 Fluorometer, Invitrogen). We obtained eight metagenomes from Lake Sarnen (deepest and shallow_inflow station for March ...
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bioRxiv - Evolutionary Biology 2022Quote: ... where an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles; Thermo Fisher) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Molecular Biology 2019Quote: ... an Acclaim PepMap 100 (2 cm x 75 μm, 3 μm particles, Thermo Fisher Scientific) pre-column was connected in front of an EASY-Spray PepMap RSLC C18 reversed phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2019Quote: ... Primer 2: 5’-GGATGTTCGTCCAGTGAGATTAG-3’) using a 7500 Fast Real-Time PCR System (Applied Biosystems) and accompanying software to analyze qPCR data.
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bioRxiv - Microbiology 2021Quote: ... an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)) assay (M6494, Thermo Fisher Scientific) was conducted in parallel to the infection and kinase inhibitor treatment according to manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...
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bioRxiv - Biochemistry 2021Quote: ... a pEntryslot3 vector containing the let-858 or tbb-2 3’UTR and pDEST (ThermoFisher) in an LR reaction ...
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bioRxiv - Biophysics 2019Quote: ... Medium was changed every 2-3 days and cells were harvested with TrypLETM Express (Invitrogen) at 80%-90% confluency ...
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bioRxiv - Cell Biology 2021Quote: siRNA transfections were performed 2 or 3 days before each experiment using Lipofectamine RNAiMax (Invitrogen) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2020Quote: ... All cell lines were passaged every 2-3 days using 0.05% Trypsin/EDTA solution (Gibco).
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bioRxiv - Cell Biology 2022Quote: ... Coverslips were prepared by incubating with a 2% solution of 3-aminopropyltrimethyoxysilane (313255000, Acros Organics) diluted in isopropanol ...