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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Recombinant Mouse Trem2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: GFP-HUVECs were added to well 1 and GFP-HUVECs co-tagged with Hoechst (NucBlue Live ReadyProbes, Invitrogen) were added to wells 2-4 ...
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Biochemistry 2022Quote: Cloning of full-length N-terminal strepII-tagged hItln1 into the pcDNA4/myc-HisA vector backbone (Life Technologies) was described previously (14) ...
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bioRxiv - Cell Biology 2023Quote: ... To visualise the cell surface with Alexa488-tagged wheat germ agglutin (Thermo Fisher Scientific, UK, 1:2000 dilution) an incubation was done prior to permeabilization ...
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bioRxiv - Molecular Biology 2023Quote: ... Genes encoding tagged and untagged SOSIP.664 Env homotrimers were expressed by transient transfection of Expi293 cells (ThermoFisher). Env heterotrimers were purified from co-transfections involving a 20:1 expression plasmid DNA ratio of untagged to tagged Env constructs ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged β-catenin variants were expressed in transient transfection assays using the pCDNA3.1 vector (Thermo Fisher Scientific). A plasmid expressing human β-catenin containing a S33Y mutation (pCDNA3-S33Y ...
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bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5’ ends were synthesized by Thermo Scientific, while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged ribosomes were then eluted by incubating the beads with 200 µg/mL HA peptide (Thermo Fisher Scientific) for 15 minutes at 30°C with constant agitation ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were transfected with plasmids encoding fluorescence-tagged PARP2 or XRCC1 using Lipofectamine 2000 (Invitrogen, Cat. 11668019) or Lonza 4D-Nucleofector™ X ...
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Biophysics 2021Quote: ... Control Drosophila melanogaster biotin-tagged kinesin-1 motors were labeled with streptavidin-conjugated Qdot 655 (Life Technologies, Carlsbad, CA) at a 1:4 motor:Qdot ratio as previously described [16 ...
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Cell Biology 2020Quote: ... The pDONR201 clones were transferred into GFP or SFB tagged DEST vectors using the Gateway LR cloning system (Invitrogen). KDM5AΔPID deletion mutant was generated by PCR in pDONR201 vector following standard cloning method and then subcloned into Gateway destination vector derived from pcDNA5/FRT/TO plasmids containing the NLS and SFB- or GFP-tagged in N-terminal ...
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bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells grown on 100-mm dishes were cotransfected with Flag-tagged ASK1_KR and PKA or empty vector using Lipofectamine 2000 (Invitrogen). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Genomics 2020Quote: ... 20.25 μg 3×Flag-tagged dCas9 plasmid and 20.25 μg sgRNA plasmid were transfected into cells using Lipofectamine 3000 (Invitrogen). Two biological replicates were performed for each condition ...
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bioRxiv - Cancer Biology 2021Quote: Control MCF-10A cells or cells expressing HA-tagged PEAK3 were cultured in -EGF medium (DMEM/F-12 (Gibco) supplemented with 0.5◻μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
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bioRxiv - Biochemistry 2021Quote: N-terminal GST-tagged MBNL1 (amino acids 1-260)59 was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... and the tagged SΔTM was purified by affinity chromatography using Ni-NTA agarose beads (Invitrogen™, Waltham, MA, USA) and size exclusion chromatography as described above.
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bioRxiv - Microbiology 2021Quote: ... bacteria were tagged by Gfp or stained by cell membrane stain FM4-64 (10 µM final concentration, Molecular Probes) for 2 mins in the dark (without flow) ...
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bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Cells at 40% confluence were transfected with 1 ug of GFP-tagged LRRK2 plasmid using Lipofectamine 2000 (Invitrogen, 11668019) as described for 12-15 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...
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bioRxiv - Microbiology 2021Quote: ... were used to pull-down FLAG-tagged proteins and any associated proteins rather than the Dynabead Protein G (Invitrogen) pre-bound with anti-FLAG M2 antibody (SigmaAldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified A-C/EBP (30 - 40 μg) was tagged with Alexa Fluor 488 according to manufacturer’s protocol (Thermo fisher). Time lag expression of A-C/EBP protein was observed using EGFP tagged A-C/EBP plasmid (pCMV-A-C/EBP-EGFP ...
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bioRxiv - Immunology 2019Quote: ... The labeled cells were counter-stained with a fluorophore-tagged secondary goat anti-rabbit IgG (H+L) antibody (Invitrogen), before acquisition on a LSR Fortessa (BD Biosciences) ...
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bioRxiv - Biophysics 2020Quote: ... The C-terminally SBP-tagged DISP1-A or DISP1-A-NNN was captured using Streptavidin-agarose affinity resin (ThermoFisher). The eluted protein was concentrated by an Amicon Ultra filter (100 kDa cutoff ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Biochemistry 2022Quote: A DNA fragment coding HA-tagged Siwi was amplified by PCR and cloned into pIZ vector (Thermo Fisher/Invitrogen) by In-fusion cloning kit (Takara) ...
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bioRxiv - Microbiology 2022Quote: ... The cells were subsequently washed with TSB and incubated with WGA tagged with Alexa Fluor 488 (WGA-488, Invitrogen) at a final concentration of 2 μg/ml for 5 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... Transgenic S2 cells carrying the SIN3 187 transgene tagged with an HA epitope were grown in medium containing 0.1 mg/ml penicillin and streptomycin (Gibco). For treatment with CuSO4 ...
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bioRxiv - Plant Biology 2023Quote: ... Epitope-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... Myc-tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Plant Biology 2023Quote: ... mYFP tagged proteins were detected by the HRP activity on SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher 34095) using a Gel Doc™ XR+ Gel Documentation System (Bio-Rad).
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bioRxiv - Molecular Biology 2022Quote: ... cells were transfected with uLimch1 and mLimch1 epitope tagged expression plasmids using Lipofectamine 3000 following the manufacturer’s protocol (catalog number L3000015, Invitrogen). mLimch1 and uLimch1 epitope tagged expression plasmids were commercially synthesized by GenScript ...
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bioRxiv - Neuroscience 2024Quote: HEK293 cells were seeded in 6-well plates and transfected with 0.25μg LgBiT-miniG (miniGs or miniGsq45) or LgBiT-β-arrestin240 and 0.25μg SmBiT-tagged receptor plasmids using 3µL Lipofectamine 2000 (Thermo Fisher). 24 hours after transfection ...
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bioRxiv - Cancer Biology 2024Quote: ... or Flag-tagged proteins were generated by inserting PCR-amplified fragments into the pcDNA3.1(+) mammalian expression vector (V79020, Invitrogen). Eukaryotic expression vectors encoding green fluorescent protein (GFP)-tagged proteins were generated by inserting PCR-amplified fragments into pd1-EGFP-N1 vector (6073-1 ...