Labshake search
Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Recombinant Mouse Trem2 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... optiMEM (1X) reduced serum medium and heat-inactivated fetal bovine serum (HI-FBS) were from Gibco chemicals ...
-
bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
-
bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
-
An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using a pcDNA 3.1-V5/His TOPO-TA cloning kit (Invitrogen K480001) per manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...
-
bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
-
bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
-
bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
-
bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
-
bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
-
bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
-
bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
-
bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...
-
bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
-
bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
-
bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
-
bioRxiv - Biochemistry 2023Quote: ... Lysates were incubated 10 minutes at 4°C with Dynabeads™ His-Tag Isolation (Invitrogen, 10103D). After one wash with NLB buffer ...
-
bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
-
bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
-
bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
-
bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... before preparation for sequencing with using Ion PGMTM Hi-QTM View Sequencing Kit (Thermo Fisher Scientific). The library was added to an Ion 318TM Chip Kit v2 BC (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... The digestion cocktail for RNase HI-negative samples was further supplemented with 120 U RNaseOUT (Invitrogen), while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen) ...
-
bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
-
bioRxiv - Molecular Biology 2020Quote: ... these PER2 cDNAs were sub-cloned into various tagged vectors including pcDNA3.0.HA (Thermo Fisher Scientific, Waltham, MA), 3XFLAG-CMV-7.1-2 (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2022Quote: ... FAM fluorescent tagged TaqMan® copy number assay probes detecting the ETNK1 gene (Hs01860263_cn) (Life Technologies, Carlsbad, CA) was used to quantify the copy number of 12p ...
-
bioRxiv - Cell Biology 2020Quote: Overexpression of mCherry-tagged actin constructs in HEK293T cells was carried out using Lipofectamine 2000 transfection reagent (ThermoFisher) at a ratio of 1:2.5 ...
-
bioRxiv - Bioengineering 2021Quote: ... The Arabidopsis eEF-1 α gene (Assay ID. No. At02337969_g1) tagged with VIC dye (Life Technologies, Carlsbad, CA) was added in each well as an internal control ...
-
bioRxiv - Biochemistry 2021Quote: ... GST-tagged TFs assayed in PBMs were detected either with Alexa488-conjugated anti-GST antibody (Invitrogen A-11131), or with a TF-specific PCRP antibody ...
-
bioRxiv - Biophysics 2021Quote: ... 0.2 µg DNA encoding GFP-tagged T-Plastin (WT or mutant) and 0.25 µL Lipofectamine2000 (Thermo Fisher Scientific), mixed in 20 µL OptiMEM (Thermo Fisher Scientific) ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The expressed C-terminal 6xHis-tagged Nluc was Ni-NTA purified using HisPurTMNi-NTA Resin (ThermoFisher scientific, #88221) as per the HisPurTMNi-NTA Resin protocol ...
-
bioRxiv - Cell Biology 2021Quote: ... AF555-and AF647-tagged secondary antibodies (donkey; 1:1000, cat. num. A-21208, A-31572, A-31571, ThermoFisher).
-
bioRxiv - Cell Biology 2022Quote: 6-dpf zebrafish larvae were gavaged with Alexa Fluor 488-tagged dextran 10,000MW (fDex) solution (Molecular Probes, USA) or purified mCherry solution (prepared in the laboratory ...
-
bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
-
bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
-
bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
-
bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
-
bioRxiv - Biochemistry 2021Quote: Flag-tagged UCH37 (WT, C88A, ΔCTD, ΔCTD/C88A, EWI, EWI/C88A) were cloned into pcDNA5/FRT/TO (Invitrogen). Stable cell lines that inducibly express these proteins were constructed from Flp-InTM T-RExTM 293 Cells following manufacturer’s instructions.
-
bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
-
bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
-
bioRxiv - Cell Biology 2020Quote: ... Inserts were then subcloned into pCS2+ N’ HA tagged vectors that had been converted into Gateway® (Invitrogen) destination vectors ...
-
bioRxiv - Molecular Biology 2020Quote: ... at a 1:5000 dilution and endogenous LC3 was tagged with an LC3B/MAP1LC3B primary antibody (ThermoFisher, L10382) used at a dilution of 1:500 for 72 hours at 4°C ...
-
bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... and the HA-glmS-tagged parasite lines were coated on diagnostic slides (Epredia; Thermo Fisher Scientific; Waltham, US) and air-dried ...
-
bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
-
bioRxiv - Evolutionary Biology 2023Quote: Lamprey embryos were injected with MYC-tagged lamprey (pou5) or Xenopus (pou5f3.1, pou5f3.2, pou5f3.3) mRNA and a FITC dextran tracer (ThermoFisher AAJ6360622) into one blastomere at the two-cell stage at the same concentration used for injections in Xenopus ...
-
bioRxiv - Biochemistry 2024Quote: Construct containing Avi-tagged HormR/GAIN domains of ADGRL3 was expressed in High Five cells (Thermo Fisher, B85502) grown in Insect-Xpress medium (Lonza ...
-
bioRxiv - Bioengineering 2024Quote: ... cells were either transduced to include a fluorescent reporter or fluorescently tagged using Hoechst (NucBlue Live ReadyProbes, Invitrogen) or general membrane cell tracker (Cellvue Claret Far Red ...
-
bioRxiv - Cell Biology 2023Quote: ... strep tagged APC/C was pre-incubated on ice with streptavidin conjugated Alexa Fluor 700 (ThermoFisher Scientific S21383) for 30 minutes ...