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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated with primary antibody (D1R: anti-D1R [mouse] 1:1500, Novus Biologicals, 48 hrs at 4°C; Th: anti-Th [rabbit] 1:1000, ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: Cell culture supernatants from cerebral cortex neurons were centrifuged at 10,000 g and 4°C and subjected either to protein precipitation or to immunoprecipitation using Reelin antibody G10 and Dynabeads Protein G (ThermoFisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... 40 μl of streptavidin magnetic beads were incubated with 4 μg of anti-CD171 (L1CAM, neural adhesion protein) biotinylated antibody (clone 5G3, #13-1719-82, Thermo Fisher) for three hours on ice ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation of sections from cerebral organoids in primary antibodies solution was performed overnight at 4°C and in secondary antibodies solution at room temperature for 1 hour (Alexa Fluor, Molecular Probes). The following primary antibodies were used ...
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bioRxiv - Cell Biology 2022Quote: ... This was followed by blocking and incubation with appropriate primary antibodies overnight at 4 °C and species specific secondary antibodies [polyclonal Alexa fluor 488 goat anti-rabbit,1:1000 (A11070 of Invitrogen Biosciences), polyclonal Alexa fluor 555 rabbit anti-mouse,1:1000 (A21427 of Invitrogen Biosciences)] for 1 hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... cells were extensively washed and incubated with secondary antibodies in the presence of 4′,6-diamidino-2-phenylindole (DAPI, Molecular Probes) for 45 min at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for two days at 4°C with rabbit monoclonal anti-GFP antibody (1:2,000; Invitrogen, Waltham, USA) or rabbit polyclonal anti-c-fos antibody (1:2,000 ...
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bioRxiv - Cancer Biology 2020Quote: ... Pre-cleared chromatin (150 μg) was incubated with 4 μl (2 μg) of monoclonal mouse anti-V5 antibody (Invitrogen R960-25) for 1 hr at 4C with rocking ...
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bioRxiv - Microbiology 2021Quote: ... and incubated with primary antibodies (1% BSA/PBS-T, overnight at 4°C) at the following dilutions: mouse anti-SAG1 (1:1000, Thermo Scientific) and rat anti-HA (1:1000 ...
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bioRxiv - Molecular Biology 2020Quote: ... overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:400, Invitrogen, Carlsbad, CA, cat. A1102) incubation for 1 hr at room temperature ...
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bioRxiv - Plant Biology 2021Quote: ... The samples were washed for 8h at 4°C with gentle agitation with PBS (0.2% v/v Tween-20) replacing the buffer every 2 hours and incubated overnight at 4°C with the appropriate secondary antibodies (ThermoFisher Scientific), anti-Rabbit-Alexa 488 (RA3) ...
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bioRxiv - Neuroscience 2021Quote: ... Tissues were then incubated overnight at 4 °C in tertiary antibody diluted in block (goat anti-chicken Alexa 488, Life Technologies, A11039 ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4 °C and with horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... primary antibodies were incubated for three nights at 4°C followed by incubation with donkey anti-mouse or anti-rabbit IgG secondary antibodies (1:1000, Life Technologies). Fluorescent images were acquired with an Olympus FV1000 at 2048 x 2048 pixel resolution using a 40x objective lens ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated for 1 h with Alexa Fluor 488-conjugated secondary antibody and nuclei were counterstained for 15 min with 4 µg/ml Hoechst 33342 (Molecular Probes). 96 and 384 well plates were scanned with Cellvoyager CV6000 (Yokogawa Inc.) ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were rinsed three times in the blocking solution and incubated for 4 hours in goat polyclonal secondary antibody to mouse IgG conjugated with AlexaFluor 555 (1:100, Invitrogen A31570) and donkey polyclonal secondary antibody to rabbit IgG conjugated with AlexaFluor 647 (1:100 ...
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bioRxiv - Cell Biology 2022Quote: ... for 1 hr at 4°C, then proceeded for immunoprecipitation with specific antibodies (Rab5A, Rab 11 and Rab7) and Protein A/G bead (Thermo Fisher) for overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cell Signaling)] at 1:1,000 in blocking buffer overnight at 4°C with rocking and subsequently with HRP-conjugated goat α-rabbit or α-mouse secondary antibodies at 1:5,000 (ThermoFisher) in blocking buffer for 2 h at room temperature with rocking ...
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bioRxiv - Systems Biology 2022Quote: ... equal amounts of protein lysates were incubated with pull-down antibody overnight at 4°C followed by 2 h treatment with Dynabeads Protein G magnetic beads (10004D, ThermoFisher, MA) for pull down ...
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bioRxiv - Developmental Biology 2020Quote: ... overnight at 4°C and further stained with 1:200 Alexa Fluor 633 Goat anti-Rabbit IgG (H+L) Secondary Antibody (Invitrogen A21071) at a 20°C for 3 hours ...
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bioRxiv - Developmental Biology 2020Quote: ... while the remainder was immunoprecipitated for 2 hr at 4°C in the following antibodies: rabbit anti-GFP (Invitrogen, A-11122), rabbit anti-Cen ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with an anti-CD63 primary antibody (1:20 dilution, Thermo Fisher Scientific, cat. # MA1-19281) in blocking buffer ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc. ...
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bioRxiv - Genomics 2020Quote: ... samples and the rest was used for immunoprecipitation overnight at 4°C with rotation with 16 μg of anti-GFP antibody (Invitrogen A11122). The chromatin was then cleared by centrifugation (12,000 g for 10 minutes at 4°C ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin with antibody were rotated overnight at 4 °C before 1:40 protein A/G agarose beads (Thermo Fisher Scientific, #20423) were added and incubated for another 6 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200) respectively at 4°C overnight and secondary antibody conjugated to Alexa Fluor 594 or Alexa Fluor 488 (Life Technologies) at room temperature for 1h ...
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bioRxiv - Developmental Biology 2020Quote: ... Sheared chromatin was cleared and incubated overnight at 4 degrees with primary antibodies that were pre-incubated with protein G magnetic DynaBeads (Thermo Fisher). Primary antibodies used included anti-H3K27Ac (9ug/sample ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...
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bioRxiv - Neuroscience 2021Quote: ... sections were washed in PBS-0.5%Triton X-100 at RT before overnight incubation at 4°C with Alexa555-conjugated donkey-anti-rabbit antibody (Invitrogen, #A32794) in PBS containing 5% NHS ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated for 2 days at 4°C with rabbit anti-GFP antibody (1:2,000; Thermo Fisher Scientific, USA). The sections were then incubated with biotinylated donkey anti-rabbit IgG antibody (1:1,000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies were washed with 1x PBS and secondary antibodies diluted in blocking solution were added 4 hrs at RT: anti-Rabbit 555 (1:800; Invitrogen; A31572), anti-Chicken Cy2 (1:800 ...
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bioRxiv - Biochemistry 2021Quote: ... resuspended in 100 μL of secondary antibody (1:500 α-mouse IgG-Alexa647 [ThermoFisher, Waltham, MA] in TBST with 4% BSA), and incubated in the dark for 60 minutes at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... or mouse monoclonal anti-N-Cadherin clone GC-4 followed by AlexaFluor 647-conjugated goat anti-mouse antibody (1:200 dilution, ThermoFisher Scientific). Staining was performed for 30 min at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were washed five times in TBSN/BSA for one hr at 4°C whilst rotating and incubated with secondary antibodies overnight at 4°C at a dilution of 1:400 (Alexa Fluor 488 goat anti-mouse (Invitrogen, #A11001) and 1:400 Poly-HRP-conjugated secondary antibody from Tyramide SuperBoost Kits with Alexa Fluor 568 (ThermoScientific ...
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bioRxiv - Immunology 2021Quote: ... targeting four different T cell populations as well as 4 μg mL−1 of goat-anti-mouse IgG AF488 secondary antibody (Thermo Scientific). Acquisition and data analysis were performed as mentioned above.
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bioRxiv - Immunology 2020Quote: ... HNSCC PBL or healthy donor PBL were stained with fluorescently labeled antibodies for 25 mins at 4°C in PBS (Thermo Fisher) supplemented with 2% FBS (Atlanta Biologicals ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% Triton X-100 in PBS) and incubated overnight (4 °C) with primary antibodies against TH (OPA1-04050 1:1000, Thermo Scientific) and HA (3F10 1:200 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed three times with PBS or PBST for 10 min and incubated with secondary antibody anti-mouse (4 μg/ml, Thermofisher #A11030) or anti-rabbit (4 μg/ml ...
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bioRxiv - Molecular Biology 2023Quote: ... then washed 4× 5min in PBS with subsequent incubation with secondary antibodies (Alexa-Fluor 488, 555, 568 or 647 (Molecular Probes or Invitrogen)) ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 hours at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... in 5% FBS overnight at 4°C and Alexa Fluor® 488 goat anti-mouse IgG secondary antibody (1:200; #10696113, ThermoFisher) for one hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... After 3 washes cells were stained with a combination of Alexa Fluor secondary antibodies and 4′,6-Diamidino-2-Phenylindole (DAPI; Molecular Probes) for 1 h at room temperature in the dark ...
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bioRxiv - Genomics 2023Quote: ... or 4 μl of rabbit polyclonal anti-GAF [101] antibodies per sample were immobilized on Protein G Dynabeads (Thermo Fisher Scientific), incubated with lysates for 90 min at 25°C ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were incubated for 2 hours with secondary antibody AlexaFluor 488 Goat Anti-Rabbit IgG (1:1000 in 4% BSA, Invitrogen #A11008) at RT in the dark ...
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bioRxiv - Developmental Biology 2023Quote: ... for one hour at RT and stained overnight at 4°C with the primary antibody rabbit anti-GFP diluted 1:500 (#A11122 Thermo Fisher). Samples were washed three times using 0.3% PBT and stained with secondary antibody Alexa Fluor 488 Goat anti-Rabbit diluted 1:1000 (#R37116 ...
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were incubated overnight at 4°C with the following antibodies: mouse anti-Beta-actin (1:1000, Thermo Fisher Scientific, AM4302) and rabbit anti-PROX1 (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... Immunoprecipitations were performed overnight at 4°C on 40μg of chromatin with antibodies pre-bound to Dynabeads protein G (Thermo Fisher Scientific). The antibodies used are listed in Table S10 ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed five times with the same blocking solution and then incubated with the corresponding secondary antibodies conjugated with Alexa fluorophores at 4°C with gentle agitation for 24 h (1:1000, Molecular Probes). Finally ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated with the respective monoclonal antibodies overnight at 4°C and detected by SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific). Membranes were exposed and visualized with the Bio-Rad ChemiDoc MP Imaging System.