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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Phospholipase A and Acyltransferase 4 RARRES3 PLAAT4 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylated peptides (Biotin-PNTAYQK(Ac)QQDTLI) previously immobilized on magnetic Streptavidin beads (Dynabeads MyOne Invitrogen™ 65601) were incubated in presence or absence of SIRT1 −/+ NAD+ (0.5 nM ...
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bioRxiv - Molecular Biology 2023Quote: ... click chemistry reactions were performed for 1.5 h in the dark (10 μM Biotin-azide [Thermo Fisher, B10184] ...
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bioRxiv - Immunology 2023Quote: ... SARS-CoV-2 RBD (BEI Resources NR-52309) was biotinylated with Sulfo-NHS-LC Biotin (Thermo Fisher), then incubated with 1μm fluorescent neutravidin beads (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... Restricted-DNA fragments were filled-in with 15 nmol each of biotin-14-dATP (cat. 19524016, Thermofisher), dTTP ...
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bioRxiv - Developmental Biology 2023Quote: Biotin-labeled cDNA was synthesized and then hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 arrays (Affymetrix) in accordance with the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... EZ-Link™ Sulfo-NHS-Biotin (100uL A39256, Thermo Fisher Scientific, 1 mg/0.4 mL in PBS). After 30min circulation ...
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bioRxiv - Molecular Biology 2024Quote: ... Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific). Concentrations given are for Ydj1 dimers and for Ssa1 and luciferase monomers.
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bioRxiv - Molecular Biology 2024Quote: ... Yd1 proteins were labeled using a 1.5-fold excess of NHS-PEG4-Biotin (Thermo Scientific, Life Technologies). Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Yd1 proteins were labeled using a 1.5-fold excess of NHS-PEG4-Biotin (Thermo Scientific, Life Technologies). Excess biotin reagent was removed using 7K molecular weight cut-off Zeba Spin Desalting Columns (Thermo Scientific) ...
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bioRxiv - Genomics 2024Quote: ... Medium was furthermore supplemented with 250 nM Rapalog 1h before harvest and 50 µm Biotin (Invitrogen, B20656) 30 min before harvest ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated in surface biotinylation buffer (0.2 mg/ml sulfo-NHS-SS-biotin in DPBS, Thermofisher#A39258) for 45 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: Primary amines of recombinant EtpA were biotinylated with EZ-Link Sulfo-NHS-LC-LC-Biotin (ThermoFisher 21388) for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... EdU was chemically linked to Biotin-azide using the Click-iT™ Reaction Kit (Thermo fisher scientific) for 1 h at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... Fluorescent conjugates were purified using Zeba™ Dye and Biotin Removal Spin Columns (Thermo Fisher Scientific®) and 50mM of Tris-HCl were added to quench the reactivity of residual dye and avoid further artifactual conjugation of the dye with EV proteins.
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bioRxiv - Neuroscience 2024Quote: ... 8.0) and incubated for 30 min with 0.8 mg/ml NHS-SS-Biotin (21327, Thermo Fisher Scientific) in ECS buffer at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... rabbit anti-rhodanese (in-house, 1/5000 dilution) and CaptureSelect biotin anti-C-tag conjugate (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The bat albumin was biotinylated using EZ-Link® NHS-SS-Biotin (Thermo Fisher Scientific, Ref#21441) following a protocol recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: ... and cells were replenished with a complete medium containing FBS and 50 uM biotin (Thermo Scientific #29129). Cells were lysed 18 hours after treatment for protein collection and analyzed by immunoblotting ...
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bioRxiv - Physiology 2024Quote: ... They were incubated with 200 μM EZ-Link™ Hydrazide-Biotin (BHZ) (Thermo Fisher Scientific, Cat#: 21339) overnight at 37°C ...
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bioRxiv - Neuroscience 2024Quote: High-capacity streptavidin agarose beads (Amid Biosciences, #SA-101-1) and custom-made biotin-conjugated QDs (ThermoFisher, per manufacturer’s specification ...
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bioRxiv - Microbiology 2024Quote: ... Recombinant VP25s from both HAstV1 and HAstV8 were biotinylated using EZ-link NHS-PEG4-Biotin (Thermo Fisher) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Biotin labelling was performed by supplementing in vitro transcription reactions with with Bio-16-UTP (Invitrogen AM8452). RNA was quantified using Qubit RNA Broad Range Assay (Invitrogen Q10211).
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bioRxiv - Genomics 2024Quote: ... Both ends of the digested chromatin were filled in and labeled with biotin-14-dATP (Life Technologies) for MboI or biotin-14-dCTP (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Biotinylation was performed by incubating purified TcdB with a 15-fold molar excess of maleimide biotin (ThermoFisher) overnight at 4°C in 20mM Tris pH7.5 ...
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bioRxiv - Developmental Biology 2024Quote: ... Biotin-tagged DNA nicks were visualized using Alexa488- or Alexa647-conjugated streptavidin (Molecular Probes, diluted 1/1000) during the incubation with the secondary antibody.
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Immunology 2020Quote: ... FcR receptor blocking antibodies were added for 15 min at 4°C and surface staining antibodies added together with live/dead stain (Fixable Live/Dead stains Thermo Fisher) and incubated for 20 mins at room temperature in the dark ...
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bioRxiv - Genetics 2021Quote: ... for 30 min at room temperature and then incubated overnight at 4°C with primary antibodies against rhodopsin (MS-1233-R7, Thermo Fisher), green opsin (AB5405 ...
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bioRxiv - Genetics 2021Quote: ... for 30 minutes after which they were incubated overnight at 4°C with a 1:25 dilution of F4/80 monoclonal rat anti-mouse antibody BM8 (ThermoFisher Scientific) or 1:100 F4/80 rat anti mouse antibody clone A3-1 (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were incubated in secondary antibodies over night at 4°C [Alexa Fluor 546 goat anti-Mouse (#A11030; Invitrogen, Carlsbad, CA)] ...
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bioRxiv - Molecular Biology 2021Quote: ... was carried out overnight at 4° C followed by secondary antibodies (conjugated with Alexa Fluor 488 or Alexa Fluor 647 (1:250; Molecular Probes, Invitrogen) at RT for 4-5 hours ...
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bioRxiv - Neuroscience 2021Quote: ... followed by incubation overnight at 4 °C in secondary antibody diluted in block (1:500 goat anti-chicken Alexa 488, Life Technologies, A11039 ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µg of protein was incubated overnight at 4°C with primary antibody as indicated (anti-AHCY [MBL RN126PW], anti-HA [Millipore 05-904], anti-IgG Rb (Invitrogen 10500C). 20 µl of Protein G Dynabeads (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then subsequently washed with PBS-0.2% Tween and incubated overnight at 4 °C in the dark with and the Alexa Fluor TM 555 Goat anti-rabbit antibody (Invitrogen #A32727), diluted 1:500 ...
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bioRxiv - Genomics 2020Quote: ... Blotting with primary antibodies was carried out at 4°C overnight followed by incubation with the appropriate secondary antibodies and developed using chemiluminescent West Pico detection kit (Thermo Scientific). Western blot quantification was carried out by subtracting the background signal from the individual bands ...
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bioRxiv - Genomics 2020Quote: ... and then incubated overnight with rocking in a humidity chamber at 4°C with anti-myc primary antibody (ThermoFisher #MA1-21316) diluted 1:1000 in blocking solution ...
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bioRxiv - Biophysics 2020Quote: ... cells were incubated in blocking buffer with 4 ng/µl of multiple-labeled goat anti-rabbit IgG secondary antibodies (Invitrogen, 31212) with different DOLs for the α-tubulin ...
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bioRxiv - Genetics 2020Quote: ... secondary antibodies diluted to 4 μg/ml in PBS containing 5 μg/ml Hoechst 33342 dye (Molecular probes, cat no. H3570). After treatment with secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Cat# ABIN6952432) overnight treatment at 4 °C followed by staining with secondary antibody Alexa-647 (goat anti-mouse, Thermo Fisher, A21235), Hoechst-33342 pentahydrate (bis-benzimide ...
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bioRxiv - Immunology 2021Quote: ... Samples were stained with surface antibody-cocktails for 20min at 4°C followed by fixable live/dead cell stain (Molecular Probes-Invitrogen). Cells were then fixed and permeabilized (eBioscience Transcription Factor Staining Buffer Kit ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then incubated overnight (4°C) in the same blocking solution containing the primary rabbit anti-PV antibody (1:1000; Thermo Scientific) and mouse anti-SST antibody (1:250 ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5-1.0 mg of cell lysate was incubated with 2-4 µg of antibody for 1 h followed by incubation with 8-10 µl of Protein G agarose (Thermo Fisher) for another 30-45 min ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in serum before incubation with mouse monoclonal β-endorphin primary antibody (Abeam ab54205,1:200) for 24 h at 4°C and Alexa-fluor 488 chicken anti-mouse secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...
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bioRxiv - Cancer Biology 2020Quote: ... or Insulin Receptor beta (ab69508) overnight at 4°C followed by anti-rabbit secondary antibody conjugated to horseradish peroxidase (ThermoFisher Scientific). Proteins were detected by Luminata Crescendo immunoblot HRP substrate (WBLUR0500Millipore Corporation ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times in HBSS/1% BSA and incubated at 4 °C for 15 minutes in the dark with the following antibodies: LDLR (1/100; MAB CL 472413; Fisher scientific), CD36 (1/10 ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
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bioRxiv - Systems Biology 2021Quote: ... Cells were washed with PBS for 4×10 min and incubated with secondary antibodies (goat anti-rabbit Alexa488 (A11034, Thermo Fisher), goat anti-mouse Alexa555 (A21424 ...
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bioRxiv - Neuroscience 2020Quote: ... then placed overnight at 4 0C in rabbit anti-GFP primary antibody (1:1000 in blocking solution, Invitrogen, La Jolla, CA). Following incubation ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... overnight at 4 °C and visualised with a fluorescent secondary antibody (goat-anti-mouse IgG Alexa 488-conjugated (1:5000, A11029, Life Technologies)) ...