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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for Fipronil Sulfone 3 Cyano Pyrazole 3 4 5 13C4 99%; 3 Cyano 5 15N2 98% 100 Ug Ml In Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cell cultures at 3 × 106/ml were transiently transfected using Expifectamine (Life Technologies) following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2019Quote: ... and 3 μg/mL of amphotericin B (Gibco Laboratories, Grand Island, NY, USA).
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bioRxiv - Bioengineering 2020Quote: ... and 3% (v/v) GlycoBlue co-precipitant (15mg/mL blue glycogen, Applied Biosystems). GlycoBlue was included in the complete lysis buffer as a hydrophilic carrier particle which associates with nucleic acids released during sample extraction ...
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bioRxiv - Cell Biology 2020Quote: Cells were first washed twice with 3 ml of Liebovitz’s medium (Life Technologies) supplemented with heat-inactivated 10% Fetal Bovine Serum (FBS) ...
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bioRxiv - Neuroscience 2020Quote: ... Hoechst 33342 was used to label cell nuclei (3 μg/ml, ThermoFisher Scientific). Sections were washed with PBS before mounting with Richard-Allan Scientific Mounting Medium (4112APG ...
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bioRxiv - Cell Biology 2024Quote: ... and cell number/ml was counted in Countess 3 (Invitrogen by Thermofisher Scientific). Cell numbers were corrected for trypan blue addition and volume of resuspension and presented as histograms.
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bioRxiv - Cell Biology 2024Quote: ... and cell number/ml was counted in Countess 3 (Invitrogen by Thermofisher Scientific). Cell numbers were corrected for trypan blue addition and volume of resuspension and presented as histograms.
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bioRxiv - Evolutionary Biology 2024Quote: ... 3 μL of 15 mg/mL GlycoBlue™ coprecipitant (ThermoFisher Scientific, MA, USA) was added and well-mixed followed by 600 μL of chilled −20 °C isopropanol ...
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bioRxiv - Bioengineering 2024Quote: ... Tissues were digested in 3 mg/mL type II collagenase (17101-015, Gibco) reconstituted in culture media for one hour at 37°C followed by digestion in 0.5 mg/mL type II collagenase overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... cells were placed under selection with medium containing 3 μg/ml puromycin (Gibco) which was replaced every 2-3 days as previously described [64].
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bioRxiv - Molecular Biology 2023Quote: ... The homogenized pellets were treated with 3 units/mL Rnase-free Dnase (Ambion) for two hours at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... plates were washed 3 times and 0.1ng/ml SA-alkaline phosphatase (ThermoFisher; 21324) in 0.1% BSA in PBS was incubated for 45 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: Purified TPPP was dialyzed overnight in 0.5–3 mL dialysis cassettes (Thermo Scientific) against PBS ...
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bioRxiv - Immunology 2023Quote: ... and the pellet resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 15-20 min at 37°C to dissociate the organoids into single cells ...
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bioRxiv - Immunology 2023Quote: ... and the pellet resuspended in 2-3 mL of TrypLE Express (Gibco # 12605010) for 12 min at 37°C to keep the LPC spheroids as aggregates ...
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bioRxiv - Cancer Biology 2023Quote: ... Following 3 days of selection with 2 µg/ml puromycin (Gibco, A11138-03), reference samples were collected (t=0 ...
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bioRxiv - Plant Biology 2023Quote: ... The cells were labeled with DiOC6(3) (Invitrogen, final concentration: 0.05 µg/ml) for 1 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: ... Spheroids were then embedded in rat-tail collagen (3 mg/ml Life Technologies). Spheroids were then cultured with unconditioned media ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 U/ml EPO (Zyrop 4000 IU injection) and 1× Pen-Strep (Gibco) as per established protocols ...
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bioRxiv - Biophysics 2023Quote: ... NucGreen™ Dead 488 (Invitrogen™, 1 drop per 3 ml in PBS); Alexa 647 Phalloidin (Invitrogen™ at 1/500) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were selected with 3 μg/ml puromycin (#A11138-03, Thermo Fisher Scientific). Single-cell clones that express similar TMEM192 levels were used in lyso-IP experiments.
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bioRxiv - Bioengineering 2023Quote: ... 330 µl of rat tail type-I collagen (3 mg/ml; Gibco A1048301) was gently diluted in 660 µl 1X PBS for a final collagen concentration of 1 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 3% calf serum and 10 μg/mL gentamicin (Thermo Fisher Scientific). When noted ...
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bioRxiv - Cell Biology 2024Quote: ... the cell media were replaced with 3 ml of FluoroBrite DMEM (#A1896702, Gibco) supplemented with 10% FBS and 10 mM HEPES (pH 7.5) ...
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bioRxiv - Bioengineering 2024Quote: ... T cells were cultured in XVivo15 based media as described above with the addition of 5 ug/mL of puromycin (Puromycin Dihydrochloride, stock concentration of 10 mg/mL in 20 mM HEPES buffer, ThermoFisher). Concentrations used in dose titration experiments ranged from 1.0 ug/mL to 10 ug/mL as indicated in Extended Data Fig ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: HeLa cell pellets (∼2×107) or MCF7 cell pellets (∼4×107) were lysed with 3 mL Mammalian Protein Extraction Reagent (Thermo Scientific), supplemented with 2X HALT protease inhibitor (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: ... The medium was changed every 2-3 days and organoids were passaged every 2-4 weeks by dissociation with 1 ml of TrypLE Express (Life Technologies) at 37°C for 5 min ...
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bioRxiv - Cancer Biology 2021Quote: ... 5’ CAGACAGUGCAGUAUUCACTT 3’) and control siRNA (#4427037) as in (56) and OSR1 siRNA (s19303 Silencer® Select) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... sonicated 3 three times for 5 sec each and analysed for protein content using the BCA protein assay kit (Thermo Fisher scientific). Samples containing 30 μl (1 μg/μl ...
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bioRxiv - Immunology 2021Quote: ... cells were washed and fed with the complete Lifeline medium plus antibiotics and transfected with 50 nM custom mirVana miRNA inhibitor (inhibitor sequence: 5′-GAGCCCGACGAGCUACCAGACUGCUCCA-3′, #4464086, Thermo Fisher Scientific) or 50 nM mirVanna inhibitor negative control#1 (#4464077 ...
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bioRxiv - Genetics 2021Quote: ... the extracted DNA was directly Sanger sequenced from in 5’ and 3’ directions for each sample with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to the manufacturer’s protocol with the same primers as for the amplification (Prdm9ZnFA_Bal_R/Prdm9ZnFA_Bal_F) ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of the extracted RNA samples were reverse-transcribed using a Uni-12 primer (5′-AGCRAAAGCAGG-3′) and Superscript III reverse transcriptase (Thermo Fisher Scientific). Ten-fold diluted cDNAs were PCR amplified using KOD FX (Toyobo ...
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bioRxiv - Cell Biology 2021Quote: ... or siGENOME SMARTpool siRNA D-019800-17 DSP (Dharmacon, Lafayette, CO) (siDP1) or stealth siRNA oligonucleotides 5′-CAGGGCUCUGUCUUCUGCCUCUGAA-3′ from Life Technologies (siDP2) using the Amaxa Nucleofector System (Lonza ...
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bioRxiv - Microbiology 2019Quote: ... and antisense primer 3Vif (5-AGCTAGTGTCCATTCATTG-3) using a Superscript III single RT-PCR system with Platinum Taq DNA polymerase kit (Thermo Fisher Scientific) as per the manufacturers instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... were co-electroporated with the gRNA-5’/3’-expressing construct and a circular donor plasmid carrying the synthesized knock-in template (GeneArt®Invitrogen™).
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bioRxiv - Molecular Biology 2021Quote: ... and 5 μL of input and 10 μL of eluate were separated on NuPAGE 3-8 % Tris-Acetate gels (Thermo Fisher Scientific) run at 4 °C and 35 mA/gel for 1.5 h ...
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bioRxiv - Cancer Biology 2019Quote: ... All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP ...
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bioRxiv - Molecular Biology 2020Quote: ... and then resuspended in aliquots of 3-5×106 CD34+ cells in a total volume of 750ul of Opti-MEM (Thermo Fisher Scientific). An aliquot of RNPs were added to the cell suspension and electroporated using the BTX ECM 830 Square Wave Electroporation System (Harvard Apparatus ...
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bioRxiv - Cell Biology 2019Quote: ... BMSCs were cultured in 12-well plate and incubated with 5 μM Fluo-3/AM dye (Invitrogen, Life Technology, Carlsbad, CA, USA) for 30 min in α-MEM at 37 °C ...
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bioRxiv - Bioengineering 2021Quote: ... and Heparin scaffold conditioned media groups (n=5) across 21 days (Days 0, 3, 7, 14, 21) using a RNAqueous™-Micro Total RNA Isolation Kit (ThermoFisher Scientific). RNA was then reverse transcribed to cDNA using a QuantiTect Reverse Transcription Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Flow settings were adjusted to allow individual cells to become encapsulated with single hydrogel beads presenting cell-specific barcoding oligonucleotide primers inside 3-5 nL encapsulation mixture droplets that contain 20 U μL−1 reverse transcriptase (SuperScript III, Thermo Fisher Scientific), First-Strand Buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: A pair of oligomers containing a 20-bp sgRNA (5’-AAAGACGAGTGATGCCATTT-3’) sequence targeting the surrounding genomic segment of HOXA9 stop codon was synthesized (Thermo Fisher Scientific) and cloned into the all-in-one vector ...
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bioRxiv - Biochemistry 2020Quote: ... Sequencing primer 5’-AAGCTGGAGCTCCACCGCGG-3’ was annealed and sequencing was performed with the USB Sequenase Version 2.0 DNA sequencing kit (Thermo Fisher Scientific - 70770).
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bioRxiv - Cancer Biology 2021Quote: ... The amplified linear products were then annealed to a 5’ adaptor with 7bp 3’ random nucleotide overhang and PCR-amplified using adaptor oligos (Sangon, Shanghai, China) and Phusion (Thermo Fisher Scientific), resulting in a library with proper Illumina sequencing adaptor ends ready for NGS ...
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bioRxiv - Cancer Biology 2021Quote: ... 4μl of the purified PCR products were added to 10μM primers and 2μM probe (forward: TACCGGTTAGTAATGAC; reverse: TAAAGCGCATGCTCCAG; probe: 5’-FAM-AAAAGCGCCTCCCCTACCCGGTAGGTA-3’-Eclipse) and 5μl TaqMan™ Fast Advanced Master Mix (Applied Biosystems™). The following thermocycling program was used ...
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bioRxiv - Cancer Biology 2020Quote: ... R: 5’-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAGCGCCTCGTGCTGGAGTAG-3’) and reacted with the gateway donor vector pDONR223 by using BP clonase II (11789020, ThermoFisher Scientific, Madrid, Spain). The resulting entry clones containing SRRM4 WT and DM were reacted with the gateway destination vector pCW57.1 (a gift from David Root ...
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bioRxiv - Systems Biology 2021Quote: ... Tissue was then washed in 1X PBS 3 × 5 min followed by incubation with Alexa Fluor 488 secondary antibody (Invitrogen, Carlsbad, CA) diluted to 1:500 in Antibody Dilution Buffer (DAKO ...
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bioRxiv - Neuroscience 2021Quote: Viral titer was estimated by qPCR with an MGB-NFQ FAM-labeled probe that recognized GFP (Life Technologies, sequence: 5’-CTTCAAGGAGGACGGCAA-3’) and 20 µg of Hsp68-EGFP vector plasmid as a standard ...
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bioRxiv - Plant Biology 2020Quote: ... qPCRs were carried out on an Applied Biosystems QuantStudio 3 and 5 Real-Time PCR Systems (Applied Biosystems, Foster City, California, USA) using 80 - 150 ng of genomic DNA as template ...
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bioRxiv - Bioengineering 2022Quote: ... PACE60 nanoparticles were formulated as described previously by double emulsion solvent evaporation using miR200b mimic (Invitrogen mirVana miRNA Mimic, hsa-miR-200b-3p, 5’-UAAUACUGCCUGGUAAUGAUGAC −3’, Cat# 4464066, Ambion, Austin, TX) or a negative control miR (Invitrogen mirVana miRNA Mimic ...