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Citations for Thermo Fisher :
1551 - 1600 of 10000+ citations for 6 Chloro N 5 ethoxy 1H pyrazol 3 yl 3 nitropyridin 2 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... Biotin labelled fragments which contained the 5’ and 3’ connecting region was enriched using Dynabeads M280 streptavidin (Invitrogen) and incubating at room temperature for 30 min ...
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bioRxiv - Microbiology 2022Quote: ... Vero-E6 cells and Calu-3 were treated with 5 μg/ml or 1 μg/ml puromycin (Gibco), respectively ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Sections were washed 3 times 5 minutes in PBS and mounted using ProLong Gold mouting media (Thermo Fisher) under glass cover slips.
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bioRxiv - Developmental Biology 2023Quote: 5’ - capped and 3’- polyadenylated hCas9-2xNLS mRNA was transcribed in vitro using mMessageMachine T7 (ThermoFisher, Cat# AM1344) and Poly(A ...
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bioRxiv - Neuroscience 2023Quote: ... Real-time PCR (RT-PCR) were performed on a QuantStudio 3 or QuantStudio 5 machines (Thermo Fisher Scientific). Primers/probe sets from Applied Biosystems were selected from the Thermo Fisher Scientific website (Supplementary Table 3) ...
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bioRxiv - Molecular Biology 2023Quote: ... An RNA linker (5’-CACGACGCUCUUCCGAUCU-3’) was ligated to the polyA RNA with T4 RNA ligase (Thermo Scientific) to capture RNAs with a 5’ monophosphate (5’P) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Slides were again washed with PBS-/- 3×5 min and mounted using Prolong Gold Antifade Mountant (Thermo Scientific). Sections stained with secondary only antibodies were included to determine fluorescence threshold before image acquisition in each channel ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were passaged in clumps at ∼ 70% confluency every 3 – 5 days by dissociation with Versene (Life Technologies) at typical ratios between 1:6 and 1:12 ...
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bioRxiv - Developmental Biology 2023Quote: ... rtf1-e3 gRNA (target sequence: 5’-GGAAGAAGGGGAAACCGAGCAA-3’) was synthesized using the MEGAshortscript T7 Transcription Kit (ThermoFisher Scientific) and purified using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Neuroscience 2023Quote: ... washed 3 x 5 min with PBS and incubated with Alexa Fluor-488 goat anti human secondary (Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were washed 3 x 5 min with TBST and developed using chemiluminescent substrate (Thermo Scientific SuperSignal West) and imaged using a BioRad Chemidoc.
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bioRxiv - Neuroscience 2023Quote: ... and then washed with PBS 3 x 5 min and mounted with ProLong Gold Antifade Mountant (ThermoFisher, P36930). Mounted coverslips were then imaged on a Zeiss Axio Observer-Z1 widefield microscope equipped with a Zeiss Plan-Apochromat 63x / 1.4 Oil objective ...
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bioRxiv - Cell Biology 2023Quote: The full length Aff3ir mRNA was cloned using commercially available 5’-RACE and 3’-RACE kits (ThermoFisher Scientific) according to the protocols provided ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were washed 3 × 5 min in PBS and embedded into ProLong Diamond Antifade Mountant (Thermo Fisher Scientific). For control experiments ...
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bioRxiv - Microbiology 2024Quote: ... exponentially growing cells in suspension were diluted to 3×109 and 5 µl spotted on 1.5% agar (Gibco) supplemented with 0.5% CTT and immediately covered with a coverslide ...
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bioRxiv - Physiology 2019Quote: ... 25 wt% N,N,N0,N0-tetrakis(2-hydroxypropyl) ethylenediamine (50-014-48142, Fisher Scientific) and 15 wt% Triton X-100 (85111 ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split every 2-3 days through 9 minutes incubation with EDTA 50mM pH=7.4 and 3 minutes of 0.25% trypsin (Gibco).
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bioRxiv - Neuroscience 2021Quote: ... was performed using the Applied Biosystems QuantStudio 3 Real-Time PCR system and analyzed with the QuantStudio 3 Design and Analysis software (v1.5.1,ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: 3′-desthiobiotin labeling was carried following the manufacturer’s’ guidelines of Pierce™ RNA 3′ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Biophysics 2019Quote: ... Procaspase-3 (0.123 mg/mL protein extract) and caspase-3 (1.0 U) were assayed in 96-well microplates (Nunc™ MicroWell™ 96-Well ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 3 µL RT reaction mix consisting of 3 µL of 50 µM random hexamers (Thermo Fisher, cat#48190011), 0.8 µL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Genomics 2021Quote: 3’-desthiobiotin labeling was carried following the manufacturers’ guidelines of Pierce™ RNA 3’ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Immunology 2020Quote: ... and 50 µL of 50 mg/mL 1-ethyl-3-[3-dimethyl-aminopropyl]-carbodiimidehydrochloride (Thermo Fisher Scientific, 22981) were simultaneously added to the reaction tubes ...
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bioRxiv - Immunology 2023Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2024Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Cancer Biology 2024Quote: ... we isolated mRNA from bone marrow of 3 Ctrl and 3 cKO animals using TRizol (Thermo Scientific, #15596026) and prepared cDNA using 500 ng of total RNA using high capacity cDNA reverse transcriptase (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and ArC Amine Reactive Compensation Beads (Invitrogen, A10346) according to manufacturer’s instruction using matching fluorophore-conjugated antibodies to the antibodies used for each instance ...
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bioRxiv - Cell Biology 2020Quote: ... siPORT™ amine (8.75 µl, Cat#AM4503, Invitrogen) and the silencer select siRNA (6 µl ...
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bioRxiv - Microbiology 2022Quote: ... and ArC Amine Reactive Compensation beads (ThermoFisher Scientific) and a known volume of CountBright absolute beads (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... All tracers were conjugated to dextran amine (Invitrogen) and had similar transport properties (85).
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bioRxiv - Bioengineering 2021Quote: ... maleic anhydride amine-binding wells (Thermo Fisher, 15100) were washed three times with PBS containing 0.05% Tween-20 ...
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bioRxiv - Immunology 2019Quote: ... and ArC™ Amine Reactive Compensation beads (Thermofisher) and manually inspected for representative samples ...
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bioRxiv - Physiology 2021Quote: ... siPORT™ amine (8.75 µl, Cat#AM4503, Invitrogen) and the silencer select siRNA (6 µl ...
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bioRxiv - Biophysics 2019Quote: 108 amine coated Dynabeads M270 (Thermo Fisher Scientific) were washed in PBS buffer pH 7.4 and incub ated in a PBS solution containing 1 % v/v glutaraldehyde for 1 h in a rotator at 18 rpm (L abnet) ...
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bioRxiv - Immunology 2023Quote: ... and ArC™ Amine Reactive Compensation Beads (Invitrogen) served as a loading control for live/dead cell staining ...
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bioRxiv - Immunology 2023Quote: ... and ArCTM Amine Reactive Compensation Bead (Thermo Fisher) individually stained with each fluorochrome ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: An amine-based protein labeling kit (Invitrogen, #A20173) was used for labeling DUNP19 with AlexaFluor-647 or AlexaFluor-594 ...
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bioRxiv - Microbiology 2020Quote: ... analyzed on a QuantStudio 3 (Applied Biosystems) thermal cycler using the standard cycling conditions ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 3 mM L-glutamine (Gibco), 0.25 mg/mL gentamycin (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3% O2 in DMEM/F12 (Gibco) with B27 supplement (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... then 3-mm glass beads (Fisher Scientific) and 1 ml fecal lysis buffer (50 mM Tris pH7.6 ...
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bioRxiv - Neuroscience 2021Quote: ... 3 % goat serum (with 0.02 % NaN3) (Invitrogen), 3 % donkey serum (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and run on a QuantStudio 3 (ThermoFisher). All reactions were normalized to the housekeeping gene RpL32 and control flies ...
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bioRxiv - Molecular Biology 2021Quote: ... on a Quant studio 3 (Applied Biosystems) Real-Time PCR Detection machine ...
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bioRxiv - Molecular Biology 2020Quote: ... 3-8% Tris-Acetate gels (Invitrogen EA0375) were used to resolve all proteins ...
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bioRxiv - Developmental Biology 2022Quote: ... To-Pro-3 (1:500, Invitrogen T3605). Secondary antibodies were obtained from Jackson ImmunoResearch or Invitrogen and used at 1:200.
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bioRxiv - Genomics 2020Quote: ... and 3 μl T4 DNA ligase (Invitrogen). All reactions were performed in LoBind tubes ...
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bioRxiv - Neuroscience 2020Quote: ... TOTO-3 (Thermo Fisher Scientific, Cat#: T3604) or DAPI (Thermo Fisher Scientific ...