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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Real-time qPCR for measuring mRNA levels was performed using Step-One-Plus real-time PCR System (Applied Biosystems) and the Fast SYBR Green Master mix (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Real-time Polymerase Chain Reaction (qPCR) was conducted in a StepOnePlus™ Real-time PCR system (Applied Biosystems®) to analyze the relative expression of the genes of inducible nitric oxide synthase (iNOS ...
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bioRxiv - Cell Biology 2020Quote: ... was used for the amplification and detection of cDNA targets on a StepOne Plus Real-time PCR system (Applied Biosystems, USA). The mean cycle threshold (Ct ...
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bioRxiv - Neuroscience 2019Quote: ... Real-time quantitative PCR was performed using the SYBR Green I assay on the ABI 7500 Sequence Detection System (Applied Biosystems). PCR reactions for each cDNA sample were performed in duplicate and copy numbers were calculated using standard curves generated from a master template as we previously described ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR was performed in three technical replicates for each sample using QuantStudio 6 Flex Real-Time PCR sequence detection system (Applied Biosystems) and FastStart Universal SYBR green Master mix (Roche) ...
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bioRxiv - Molecular Biology 2021Quote: ... real-time PCR of RNA templates using hybridization probe detection chemistries (e.g., TaqMan® 5’-hydrolysis probes) on ABI7500 (Applied Biosystems) was used ...
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bioRxiv - Genomics 2019Quote: ... and hybridized to a custom probe set for expression analysis on the nCounter Digital Analyzer (NanoString Technologies) or cDNA-converted for detection on the 7900HT Fast Real-time PCR System (Applied Biosystems).
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bioRxiv - Pharmacology and Toxicology 2021Quote: Temperature control and fluorescence detection were performed using a Step One Plus Real-Time PCR System (Applied Biosystems; Grand Island, NY) with indicator dye (GloMelt ...
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bioRxiv - Genetics 2019Quote: ... Real-time PCR was performed in an optical 96-well plate with an ABI PRISM 7500 sequence detection system (Applied Biosystems) and fast cycling conditions (20s at 95°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Differences in the DNA content from every immunoprecipitation assay were determined by real-time PCR using the ABI 7700 sequence detection system and SYBR Green master mix protocol (Applied Biosystems). Primers used in this study are listed in table S1 ...
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bioRxiv - Microbiology 2022Quote: ... Real-time PCR detection of the ATI gene of MPXV was performed using TaqMan Gene Expression Master Mix (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... following the manufacturer’s protocol and qPCR carried out with a CFX96 Touch Real-Time PCR Detection System using SYBR Green mix (Life Technologies). Primer sequences for qRT-PCR for mouse mRNAs were obtained from PrimerBank (https://pga.mgh.harvard.edu/primerbank/) ...
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bioRxiv - Microbiology 2023Quote: ... PCR amplifications were carried out for 40 cycles using serially diluted DNA samples (mock, IP treated and total input) in a real-time PCR detection system (Applied Biosystems). In all cases melting curve analysis confirmed amplification of a single product.
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bioRxiv - Plant Biology 2022Quote: ... Gene expression was accessed using real-time PCR (qRT-PCR) (Step One PlusTMReal Time PCR System, Applied Biosystems, CA, USA) with the SYBR green fluorescence detection (Applied Biosystems®) ...
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bioRxiv - Neuroscience 2023Quote: ... Thermo Fisher) and the Applied BiosystemsTM ViiA Real-Time PCR Systems by relative quantitative RT-PCR (Thermo Fisher Scientific, Waltham, MA; Applied Biosystems, Foster City, CA) and expression fold change was calculated using the ΔΔCq calculation method ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR was then performed using the Applied Biosystems StepOne™ system with SYBR Green RT-PCR Master Mixes (Thermo Fisher Scientific, cat: #A25742). The PrimePCR SYBR green assay primers (see Table 4 ...
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bioRxiv - Neuroscience 2021Quote: ... and placed in the QuantStudio 6 Pro Real-Time PCR system (all reagents for RT-qPCR were purchased from ThermoFisher Scientific). The PCR cycle protocol was ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real time quantitative PCR(RT-qPCR) was performed to analyse relative gene expression levels using SYBR Green Master mix (Applied Biosystems) following manufacturer indications ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was prepared from 200 ng of total RNA using SuperScript® III First-Strand Synthesis System for real-time polymerase chain reaction (RT-PCR) (Cat. No. 18080-051, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of pobA and 16S rRNA transcripts (primers 341f/534r) was performed by RT-qPCR using an AB7300 Real-Time PCR system (Applied Biosystems) as described previously [65] (details in Supplementary Methods) ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for RT-qPCR and samples were quantified on QuantStudio™ 12K Flex Real-Time PCR System (Thermo Fisher Scientific). In brief ...
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bioRxiv - Microbiology 2022Quote: Cell RNA isolation and RT–qPCR was performed using procedures described previously [36] using the 7900HT Fast Real-Time PCR System (Applied Biosystems). Primers for GAPDH ...
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bioRxiv - Developmental Biology 2022Quote: ... The synthesized cDNA was then used for RT-qPCR using a StepOne real-time PCR system (Applied Biosystems, Waltham, MA, USA) with the KOD SYBR qPCR Mix (Toyobo Co ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... according to the manufacturer’s protocol using a StepOne Plus real-time polymerase chain reaction (RT-PCR) (Applied Biosystems, Foster City, CA). Relative gene expression of hypertension and vascular remodeling genes ...
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bioRxiv - Physiology 2020Quote: ... RT-qPCRs were performed on an Applied Biosystems 7500 Real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA), with the following cycling conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-qPCR for miRNA was performed using TaqMan Universal PCR Master Mix II (Applied Biosystems, Foster City, CA, USA) with specific probes (Life Technologies 4440887 assay numbers 000377 (let-a) ...
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bioRxiv - Microbiology 2019Quote: ... The extracted RNA was reversely transcribed and amplified by a one-step RT-qPCR using a 48-well plate real-time PCR system Step-One (Applied Biosystems). The sets of primers and probes used for the VP1 and for the Elongation Factor −1 alpha (ELF1α ...
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bioRxiv - Plant Biology 2021Quote: RT-qPCR was performed with the ABI Prism 7900 HT fast real time PCR system (Applied Biosystems/Life Technologies, Darmstadt, Germany) using a Power SYBR® Green-PCR Master Mix (Applied Biosystems/Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: RT-qPCR was performed with the ABI Prism 7900 HT fast real time PCR system (Applied Biosystems/Life Technologies, Darmstadt, Germany) using a Power SYBR® Green-PCR Master Mix (Applied Biosystems/Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... All RT-qPCR reactions were performed in triplicate on a QuantStudio5 Real-Time PCR System (Thermo Fisher Scientific, Waltham, United States), and reaction conditions were as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: Extracted RNA was diluted to 5ug/ml and subjected to one step RT-qPCR on the StepOnePlus(tm) Real-Time PCR System (Applied Biosystems) using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using the RT2 First Strand Kit according to the manufacturer’s instructions and used as template for quantitative RT-qPCR with SYBR Green Master Mix and a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). Due to the low amount of RNA extracted from astrocytes on transwell ...
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bioRxiv - Neuroscience 2022Quote: ... along with a minus reverse transcriptase control (-RT) were ran for each gene on the QuantSudio 6 Flex Real-Time PCR System (Applied Biosystems). The -RT served as a negative control ...
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bioRxiv - Immunology 2022Quote: ... Expression levels of diverse transcripts were analyzed by real-time RT-qPCR using Power SYBR green PCR master mix (Applied Biosystems) and various primer sets (Suppl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and real-time quantitative RT-PCR was performed with the PowerTrack™ SYBR Green Master Mix (Thermo Fisher, Waltham, MA USA). Sequences of genes as listed in supplementary table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed using KOD SYBR qPCR Mix (Toyobo) on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed in technical quadruplets on a QuantStudio 6 Flex Real-Time 384-well PCR System (Applied Biosystems, 4485694) using PowerUp SYBR Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: The RT-qPCR reactions were carried out on 384-well plates using a QuantStudio 7 Flex Real- Time PCR System (ThermoFisher #4485701) using 10 µl reactions (2 µl of cDNA 3.7 ng/µl ...
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bioRxiv - Biochemistry 2023Quote: ... Real time analyses by two-step RT-PCR were performed for quantification of miRNA levels on Bio-Rad CFX96TM real time system using Taqman (Applied Biosystems) based miRNA assay system ...
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bioRxiv - Plant Biology 2023Quote: ... 50 ng cDNA was used as a template for RT-qPCR using the 7500 fast real-time PCR system (Applied Biosystems). Data were analyzed by the comparative threshold cycle method (ΔCT methods) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and real-time quantitative RT-PCR was performed with the PowerTrack™ SYBR Green Master Mix (Thermo Fisher, Waltham, MA USA). Sequences of genes as listed in supplementary table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Levels of MET and target gene mRNAs were evaluated by real-time RT-PCR with Fast SYBR Green mix (Applied Biosystems). Relative gene expression levels were calculated using the 2-DCt method ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA isolated from cells with high purity was used to analyze gene expression by RT-qPCR performed on a Step One Plus Real-Time PCR System using TaqMan gene assay reagents (Applied Biosystems), according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2021Quote: ... and quantitative real-time PCR was performed using TaqMan® Fast Advanced Master Mix in a StepOnePlus™ real-time PCR system (Applied Biosystems, USA). Quantification of each mRNA was carried out with Actb or Ubc as reference genes ...
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bioRxiv - Microbiology 2021Quote: ... Quantification of vRNA load was performed by real-time PCR using the Taqman amplification system and the QuantStudio 12 K Flex Real-Time PCR System (ThermoFisher Scientific, Grand Island, NY) as described previously (43) ...
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bioRxiv - Immunology 2022Quote: ... and quantitative real-time polymerase chain reactions (qRT-PCR) were analyzed on an Applied Biosystems 7500 Fast Real-Time PCR System (ThermoFisher Scientific, Eugene, OR, USA) using Hieff® qPCR SYBR® Green Master Mix (Yeasen ...
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bioRxiv - Neuroscience 2022Quote: ... were examined by real-time PCR analysis using a StepOnePlus real-time PCR system (Applied Biosystems, Life Technologies, Thermo Fisher Scientific Inc., Waltham, MA). Gapdh was used as the internal control (forward primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative real-time PCR (qPCR) reactions were performed using Fast SYBR® Green reagents in a StepOnePlus™ Real-Time PCR system (Applied Biosystems™) with a +0.7°C melt increment ...
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bioRxiv - Microbiology 2021Quote: ... A TaqMan-based real-time PCR was then performed on the DNA samples using the QuantStudio 3 Real-Time PCR Systems (ThermoFisher Scientific, Waltham, MA, USA), using optical grade 96-well plates ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was amplified using SYBR Green-based real-time PCR and the QuantStudio™ 12K Flex Real-Time PCR Systems (Applied Biosystems™, USA) following manufacturers protocol ...