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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for hsa mir 23b Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... and placed in the QuantStudio 6 Pro Real-Time PCR system (all reagents for RT-qPCR were purchased from ThermoFisher Scientific). The PCR cycle protocol was ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Real time quantitative PCR(RT-qPCR) was performed to analyse relative gene expression levels using SYBR Green Master mix (Applied Biosystems) following manufacturer indications ...
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bioRxiv - Neuroscience 2019Quote: ... cDNA was prepared from 200 ng of total RNA using SuperScript® III First-Strand Synthesis System for real-time polymerase chain reaction (RT-PCR) (Cat. No. 18080-051, Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Quantification of pobA and 16S rRNA transcripts (primers 341f/534r) was performed by RT-qPCR using an AB7300 Real-Time PCR system (Applied Biosystems) as described previously [65] (details in Supplementary Methods) ...
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bioRxiv - Cancer Biology 2022Quote: ... was used for RT-qPCR and samples were quantified on QuantStudio™ 12K Flex Real-Time PCR System (Thermo Fisher Scientific). In brief ...
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bioRxiv - Microbiology 2022Quote: Cell RNA isolation and RT–qPCR was performed using procedures described previously [36] using the 7900HT Fast Real-Time PCR System (Applied Biosystems). Primers for GAPDH ...
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bioRxiv - Developmental Biology 2022Quote: ... The synthesized cDNA was then used for RT-qPCR using a StepOne real-time PCR system (Applied Biosystems, Waltham, MA, USA) with the KOD SYBR qPCR Mix (Toyobo Co ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... according to the manufacturer’s protocol using a StepOne Plus real-time polymerase chain reaction (RT-PCR) (Applied Biosystems, Foster City, CA). Relative gene expression of hypertension and vascular remodeling genes ...
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bioRxiv - Physiology 2020Quote: ... RT-qPCRs were performed on an Applied Biosystems 7500 Real-time PCR system (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA), with the following cycling conditions ...
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bioRxiv - Cancer Biology 2020Quote: ... Real-time RT-qPCR for miRNA was performed using TaqMan Universal PCR Master Mix II (Applied Biosystems, Foster City, CA, USA) with specific probes (Life Technologies 4440887 assay numbers 000377 (let-a) ...
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bioRxiv - Microbiology 2019Quote: ... The extracted RNA was reversely transcribed and amplified by a one-step RT-qPCR using a 48-well plate real-time PCR system Step-One (Applied Biosystems). The sets of primers and probes used for the VP1 and for the Elongation Factor −1 alpha (ELF1α ...
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bioRxiv - Plant Biology 2021Quote: RT-qPCR was performed with the ABI Prism 7900 HT fast real time PCR system (Applied Biosystems/Life Technologies, Darmstadt, Germany) using a Power SYBR® Green-PCR Master Mix (Applied Biosystems/Life Technologies ...
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bioRxiv - Plant Biology 2021Quote: RT-qPCR was performed with the ABI Prism 7900 HT fast real time PCR system (Applied Biosystems/Life Technologies, Darmstadt, Germany) using a Power SYBR® Green-PCR Master Mix (Applied Biosystems/Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... All RT-qPCR reactions were performed in triplicate on a QuantStudio5 Real-Time PCR System (Thermo Fisher Scientific, Waltham, United States), and reaction conditions were as follows ...
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bioRxiv - Evolutionary Biology 2022Quote: Extracted RNA was diluted to 5ug/ml and subjected to one step RT-qPCR on the StepOnePlus(tm) Real-Time PCR System (Applied Biosystems) using the Luna® Universal One-Step RT-qPCR Kit (New England Biolabs) ...
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bioRxiv - Neuroscience 2023Quote: ... cDNA was synthesized using the RT2 First Strand Kit according to the manufacturer’s instructions and used as template for quantitative RT-qPCR with SYBR Green Master Mix and a QuantStudio 5 Real-Time PCR System (ThermoFisher Scientific). Due to the low amount of RNA extracted from astrocytes on transwell ...
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bioRxiv - Neuroscience 2022Quote: ... along with a minus reverse transcriptase control (-RT) were ran for each gene on the QuantSudio 6 Flex Real-Time PCR System (Applied Biosystems). The -RT served as a negative control ...
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bioRxiv - Immunology 2022Quote: ... Expression levels of diverse transcripts were analyzed by real-time RT-qPCR using Power SYBR green PCR master mix (Applied Biosystems) and various primer sets (Suppl ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and real-time quantitative RT-PCR was performed with the PowerTrack™ SYBR Green Master Mix (Thermo Fisher, Waltham, MA USA). Sequences of genes as listed in supplementary table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed using KOD SYBR qPCR Mix (Toyobo) on a QuantStudio 5 Real-Time PCR System (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... RT-qPCR was performed in technical quadruplets on a QuantStudio 6 Flex Real-Time 384-well PCR System (Applied Biosystems, 4485694) using PowerUp SYBR Green Master Mix (ThermoFisher Scientific ...
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bioRxiv - Genomics 2023Quote: The RT-qPCR reactions were carried out on 384-well plates using a QuantStudio 7 Flex Real- Time PCR System (ThermoFisher #4485701) using 10 µl reactions (2 µl of cDNA 3.7 ng/µl ...
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bioRxiv - Biochemistry 2023Quote: ... Real time analyses by two-step RT-PCR were performed for quantification of miRNA levels on Bio-Rad CFX96TM real time system using Taqman (Applied Biosystems) based miRNA assay system ...
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bioRxiv - Plant Biology 2023Quote: ... 50 ng cDNA was used as a template for RT-qPCR using the 7500 fast real-time PCR system (Applied Biosystems). Data were analyzed by the comparative threshold cycle method (ΔCT methods) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and real-time quantitative RT-PCR was performed with the PowerTrack™ SYBR Green Master Mix (Thermo Fisher, Waltham, MA USA). Sequences of genes as listed in supplementary table 1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Levels of MET and target gene mRNAs were evaluated by real-time RT-PCR with Fast SYBR Green mix (Applied Biosystems). Relative gene expression levels were calculated using the 2-DCt method ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA isolated from cells with high purity was used to analyze gene expression by RT-qPCR performed on a Step One Plus Real-Time PCR System using TaqMan gene assay reagents (Applied Biosystems), according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2021Quote: ... and quantitative real-time PCR was performed using TaqMan® Fast Advanced Master Mix in a StepOnePlus™ real-time PCR system (Applied Biosystems, USA). Quantification of each mRNA was carried out with Actb or Ubc as reference genes ...
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bioRxiv - Microbiology 2021Quote: ... Quantification of vRNA load was performed by real-time PCR using the Taqman amplification system and the QuantStudio 12 K Flex Real-Time PCR System (ThermoFisher Scientific, Grand Island, NY) as described previously (43) ...
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bioRxiv - Immunology 2022Quote: ... and quantitative real-time polymerase chain reactions (qRT-PCR) were analyzed on an Applied Biosystems 7500 Fast Real-Time PCR System (ThermoFisher Scientific, Eugene, OR, USA) using Hieff® qPCR SYBR® Green Master Mix (Yeasen ...
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bioRxiv - Neuroscience 2022Quote: ... were examined by real-time PCR analysis using a StepOnePlus real-time PCR system (Applied Biosystems, Life Technologies, Thermo Fisher Scientific Inc., Waltham, MA). Gapdh was used as the internal control (forward primer ...
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bioRxiv - Cancer Biology 2019Quote: ... Quantitative real-time PCR (qPCR) reactions were performed using Fast SYBR® Green reagents in a StepOnePlus™ Real-Time PCR system (Applied Biosystems™) with a +0.7°C melt increment ...
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bioRxiv - Microbiology 2021Quote: ... A TaqMan-based real-time PCR was then performed on the DNA samples using the QuantStudio 3 Real-Time PCR Systems (ThermoFisher Scientific, Waltham, MA, USA), using optical grade 96-well plates ...
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bioRxiv - Microbiology 2021Quote: ... The resulting cDNA was amplified using SYBR Green-based real-time PCR and the QuantStudio™ 12K Flex Real-Time PCR Systems (Applied Biosystems™, USA) following manufacturers protocol ...
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bioRxiv - Pathology 2019Quote: ... Quantitative real-time PCR was performed using SYBR Green Master Mix in the QuantStudio 12k Flex Real-Time PCR system (Life Technologies, Carlsbad, CA, USA) to validate DEGs ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR reactions were performed in duplicate using the TaqMan® system in a StepOne Plus® real-time PCR system (StepOne Plus® Real-Time PCR System, Applied Biosystems) and the following cycle program ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA products were run in triplicates for Quantitative real time PCR using a QuantStudio 5 Real-time PCR System (Applied Biosystems, cat no: 4368814) and Powerup SYBR green master mix (Applied Biosystems ...
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bioRxiv - Developmental Biology 2023Quote: ... and various sets of gene-specific forward and reverse primers and subsequently subjected to real-time PCR quantification using a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). The following primer sequences were used ...
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Phospholipase C β4 promotes RANKL-dependent osteoclastogenesis by interacting with MKK3 and p38 MAPKbioRxiv - Cell Biology 2024Quote: ... Quantitative real-time PCR was performed using an ABI 7500 real-time PCR system and SYBR Green dye (Applied Biosystems, Foster City, CA, USA). The mRNA expression levels were normalized to the endogenous GAPDH housekeeping gene ...
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bioRxiv - Cancer Biology 2024Quote: ... Quantitative real-time PCR was performed on an ABI PRISM 7900HT Fast Real-Time PCR machine using SYBR Green (Applied Biosystems, Foster City, CA). Data was analyzed with SDS 2.3 software ...
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bioRxiv - Physiology 2019Quote: ... Real-time quantitative polymerase chain reaction (RT-qPCR) was performed on a StepOnePlus RT-qPCR machine (Applied Biosystems) with each reaction consisting of triplicate samples containing iTaq Universal Probes Supermix (BioRad) ...
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bioRxiv - Immunology 2021Quote: ... Real-time qRT-PCR was performed using SuperScript™ III Platinum™ One-Step qRT-PCR Kit (Invitrogen, USA) following the manufacturer’s instructions to detect NDV M gene64 and enable the calculation of NDV genome copies.
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bioRxiv - Developmental Biology 2023Quote: ... Real-time PCR was performed using an ABI 7500 thermocycler with Power SYBR Green PCR Kit (Thermo Fisher, 4368702). The relative expression of interested genes is normalized to the expression of 18S rRNA or β-actin ...
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bioRxiv - Cell Biology 2020Quote: ... PCR was performed on the ABI 7900HT Real-Time PCR system (Applied Biosystems) using SYBR Green Master mix (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... All PCRs were run on a QuantStudio Real-time PCR machine (Applied Biosystems).
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bioRxiv - Developmental Biology 2020Quote: ... Quantitative real time PCR was performed on a StepOnePlus PCR machine (Applied Biosystems) using the FastStart Universal SYBR Green Master (Roche ...
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bioRxiv - Plant Biology 2019Quote: ... qRT-PCR was performed in 7500 Real-Time PCR System (Applied Biosystems, USA) using 1/20 times dilution of the respective cDNAs ...
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bioRxiv - Neuroscience 2020Quote: ... qRT-PCR plates were run on StepOnePlus Real-Time PCR machines (Applied Biosystems) and data was initially analyzed using StepOne Software (Applied Biosystems) ...
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bioRxiv - Systems Biology 2020Quote: ... qRT-PCR was performed using the ViiA7 real-time PCR system (Applied Biosystems). Primers were generated using the Primer-BLAST design tool from NCBI based on the accession number of the gene of interest ...
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bioRxiv - Genetics 2020Quote: ... qRT-PCR was performed using a ViiA7 Real-Time PCR System (Applied Biosystems) (40 cycles at 95 °C for 15 sec and 60 °C for 1 min) ...