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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Tubulin gamma Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2023Quote: Recombinant hTMEM87A proteins (WT and mutants) were transiently expressed in Expi293F cells (#A14527, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant RBD-hFc proteins were captured by Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), washed by wash buffer (100 mM Tris/HCl ...
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bioRxiv - Bioengineering 2023Quote: ... EGF at a concentration of 10 ng/ml (Recombinant Human EGF, Gibco, Thermo Fisher Scientific, PHG0314), Prinomastat® at a concentration of 10 nM [24] (Sigma Aldrich ...
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bioRxiv - Evolutionary Biology 2023Quote: Recombinant PrPAP1Xa was secretively expressed using the Bac-to-Bac™ Baculovirus Expression System (Invitrogen, USA) as previously described22 ...
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bioRxiv - Biophysics 2023Quote: ... and 5 ng/mL prequalified human recombinant epidermal growth factor 1-53 (EGF 1-53; Gibco). Hippocampal neurons were grown in neuro basal medium ...
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bioRxiv - Genetics 2023Quote: ... and the random primer 5′-GTTTCCCAGTCACGATCNNNNNNNNNNNNNNN-3′ for the RT step and recombinant Taq polymerase (Invitrogen) for the PCR step using D388 specific primers 5′-GAGAGAAGGATTAGATTGTG-3′ and 5′-CCTGTATAATAGAAGTACCA-3′ ...
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bioRxiv - Immunology 2023Quote: Recombinant Omicron RBD proteins from variants BA.2 and BA.4.5 were obtained from Fisher Scientific. To determine the specificity of mAbs ...
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bioRxiv - Cancer Biology 2023Quote: ... containing 150 ng/µl sgRNA and 500 ng/ul recombinant TrueCut Cas9 protein (Thermo Fisher, A36496) using Invitrogen Neon Electroporator with 2 pulses of 1200 V ...
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bioRxiv - Bioengineering 2024Quote: ... and 10 ng/mL recombinant Activin-A (338-AC, Bio-Techne) in RPMI 1640 (61870036, Gibco) with added B-27 supplemented minus insulin (12587010 ...
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bioRxiv - Biochemistry 2024Quote: ... Recombinant proteins were purified under denaturing conditions (8 M urea) using Ni-NTA resin (Thermo Scientific). The purity of the recombinant proteins was assessed via SDS-PAGE ...
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bioRxiv - Immunology 2024Quote: ... and containing recombinant human stem cell factor (h-SCF; Gibco, PeproTech, #300-07; 100 ng/mL) and human IL-6 (Gibco ...
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bioRxiv - Biochemistry 2024Quote: The recombinant baculovirus was prepared using the Bac-to-Bac baculovirus expression system (Thermo Fisher Scientific). For expression ...
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bioRxiv - Microbiology 2024Quote: ... the recombinant section of the hisC::’ND5i’ allele was amplified by PCR using DreamTaq (Thermo Scientific) and primers hisC-ins-f/hisC-ins-r (Table 2) ...
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bioRxiv - Cancer Biology 2024Quote: ... and rabbit anti-GFP (polyclonal anti-GFP rabbit IgG, A11122 Invitrogen, 1:000 ...
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bioRxiv - Cancer Biology 2021Quote: ... rabbit antibodies were coupled to magnetic beads conjugated to anti-rabbit IgG (Dynabeads Sheep anti-rabbit IgG, Invitrogen, #11203D). The buffer containing 200 mM NaCl ...
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bioRxiv - Immunology 2021Quote: ... hybridoma cells were adapted to and expanded in serum free medium containing 1:2 vol/vol Cell™ MAB medium (Life Technologies) and HyClone™ CDM4MAB medium (GE Life Sciences) ...
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bioRxiv - Microbiology 2020Quote: ... Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems) in casein blocking buffer (Thermo Fisher Scientific) for 90 min ...
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bioRxiv - Microbiology 2022Quote: ... recovered sporozoites cells were stained with anti-PvCSP mAbs 1:500 and fixed 10 min in 100uL of a 2% PFA solution (Thermo Scientific™) in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... They were stained for 2 days at 4°C with a mouse-derived antibody against GFP (1:400, mAB 3E6, Invitrogen, Darmstadt, Germany), washed for 2 hours at RT in 1x PBS ...
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bioRxiv - Immunology 2022Quote: The binding capacity of the glycoengineered COVA1-18 (2C1) hIgG1 mAbs was tested by directly coating Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific) O/N at 4°C with 100 µL 1 µg/mL purified SARS-CoV-2 RBD-protein ...
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bioRxiv - Immunology 2022Quote: ... OEP light cages were applied to export individual cross-reactive mAb secreting B cells directly into Dynabeads mRNA DIRECT lysis buffer (Life Technologies, 61011) in 96-well plates ...
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bioRxiv - Microbiology 2022Quote: ... The internalization of the ACE2-HA protein and anti-hACE2 mAbs was then evaluated by staining with goat anti-mouse Alexa Fluor™ 594 (to detect the HA-tagged hACE2) and/or goat anti-human Alexa Fluor™ 488 antibodies (to detect the hACE2 mAbs) (ThermoFisher Scientific). Images were captured using a DeltaVision OMX SR imaging system (GE Healthcare).
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bioRxiv - Immunology 2020Quote: ... the unbound xfR5 mAb was washed off by dialysis using Float-A-Lyzer™ G2 Dialysis Devices (Thermo Fisher Scientific; NH, USA) in cold PBS supplemented with 10mM hydroxylamine to quench any non-reacted NHS groups present on the xfR5 mAb conjugated D+T NPs (xfR5-D+T NPs ...
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bioRxiv - Microbiology 2021Quote: ... operated by UNICORN software version 5.11 (Build 407) using HiTrap Protein A columns (Cytiva) for full length human mAbs and CaptureSelect CH1-XL MiniChrom columns (Thermo Fisher Scientific) for Fab fragments ...
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Development of follicular dendritic cells in lymph nodes depends on retinoic acid mediated signalingbioRxiv - Immunology 2020Quote: ... and unlabeled anti-LTβR mAb and subsequently counterstained with streptavidin-Alexa 488 and goat-anti-rat-Alexa 488 (all Invitrogen, Breda, The Netherlands), respectively ...
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bioRxiv - Microbiology 2021Quote: ... Permeabilized cells were probed with a monoclonal antibody against the HA-tag epitope (1:1000; HA Tag mAb 2-2.2.14, Thermo Fisher Scientific, Planegg, Germany) to detect SARS-2-S protein ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once before being stained with surface mAb (Table S1) and viability dye LIVE/DEAD Fixable Near-IR (Thermo-Fisher Scientific, USA) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... T-cells were incubated overnight at 4 °C with either mAb reactive to mouse phospho-CD247 (CD3 zeta) (Tyr111, Tyr123) (clone EM-55; Thermo Fisher Scientific) or mAb reactive to mouse ZAP70 phosphorylated at position 319 (clone 1503310 ...
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bioRxiv - Immunology 2023Quote: ... and next intracellularly stained with PE-conjugated anti-human phospho-Akt (Ser473) (D9E, CST) and anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) manually conjugated with Alexa 488 or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Biochemistry 2023Quote: ... Detection of bound Af2-IL was performed using a rat anti-human IL-2 mAb Alexa Fluor488 conjugate (Invitrogen, Carlsbad CA, USA). Cell nuclei were counterstained with DAPI (Sigma Aldrich ...
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bioRxiv - Microbiology 2023Quote: Monoclonal antibodies (mAbs) targeting β-GlcNAc-WTA (clone 4497) in different Ig isotypes and IgG subclasses were expressed in EXPI293F cells (Thermo Fisher) as previously described (29 ...
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bioRxiv - Immunology 2023Quote: ... anti-SIVmac251 Nef mAb (clone 17, epitope peptide corresponding to SIVmac239 Nef 71-80 not including the residue G63: Thermo Scientific/Pierce) manually conjugated with Alexa 488 or Alexa 647 ...
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bioRxiv - Immunology 2023Quote: ... they were resuspended in 0.5% BSA/PBS for prevention of experimental procedure-related loss and stained with mouse anti-SIVmac251 Nef mAb (clone 17, Thermo Scientific/Pierce) or mouse IgG1 isotype control mAb (P3.6.2.8.1 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then incubated in the dark for 30-45 min on ice with goat anti-mouse fluorochrome conjugated isotype specific mAbs (Life Technologies Corporation, Invitrogen, USA), washed twice and re-suspended in 2% BSA PBS ...
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bioRxiv - Immunology 2023Quote: ... were coated with 10 µg/mL of anti-LAIR-1 agonist mAb (clone Dx26) or 10 µg/mL of mouse isotype control IgG1 (eBioscience-Thermo Fisher Scientific) diluted in PBS overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoprecipitations (IPs) were also performed from the same lysates with the (Neuromab) antibody mAb-α-iFGF14 conjugated to protein G dynabeads (Life Technologies), using previously described methods (Bosch et al. ...
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bioRxiv - Immunology 2024Quote: ... the mAbs following expression were purified and subjected to SDS-PAGE analysis using a NuPAGE 4-12% Bis-Tris gel (Thermo Scientific Inc) under reducing (lane 1 ...
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Polo-like kinase 1 independently controls microtubule-nucleating capacity and size of the centrosomebioRxiv - Cell Biology 2020Quote: ... elegans γ-tubulin complex was reconstituted by co-expression in human FreeStyle 293-F cells (Thermo Fisher Scientific). The GIP-2 and γ-tubulin (TBG-1 ...
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bioRxiv - Biochemistry 2021Quote: ... for spike protein or RBD detection and mouse anti-beta tubulin antibody (1:5000; Thermo Fisher; MA5-16308) as a loading control ...
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bioRxiv - Genetics 2022Quote: ... γ-tubulin (ciliary basal body marker; SIGMA cat. no. T5192; secondary antibody Invitrogen A27034 labeled with Alexa Fluor 488) or eGFP (Aves cat ...
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bioRxiv - Biochemistry 2019Quote: ... A similar method was used to conjugate biotin to tubulin (EZ-Link™ NHS-PEG4-Biotin, ThermoFisher: 21329). Conjugated tubulin was further purified for MT competent tubulin by a series of MT polymerization and pelleting rounds ...
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bioRxiv - Cell Biology 2019Quote: ... rat monoclonal anti-α-tubulin (YL1/2; 1:1000 for IF; Cat#MA1-80017, Thermo Fisher, Waltham, MA), FITC-conjugated α-tubulin (DM1A ...
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bioRxiv - Biochemistry 2022Quote: ... Primary cilia were visualized by staining with anti-acetylated alpha tubulin (1:400, Invitrogen, catalog no. 32-2700) and imaged using a Zeiss Axioplan microcope equipped with a Leica DMC6200 camera ...
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bioRxiv - Cell Biology 2024Quote: ... The primary antibodies used in this work were mouse monoclonal anti-tubulin (DM1α; Thermo Fisher Scientific; 1:200) and rabbit anti-GFP (NB600-308 ...
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bioRxiv - Microbiology 2023Quote: ... Dm0 (DSHB, ADL67.10), α-Tubulin (Beyotime, AF0001), Flag tag (HUABIO, 0912-1) and GP64 (Invitrogen, 14-6995-85) were used.
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bioRxiv - Bioengineering 2024Quote: ... and anti-acetylated tubulin staining: 4 mL blocking buffer containing 8 μL Alexa Fluor 488 (Invitrogen, A-11006), 8 μL Alexa Fluor 594 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were incubated overnight on a wheel in a cold room with protein A magnetic beads coupled with 1ug of rabbit anti-SRGAP2A or 1ug of rabbit anti-CTNND2 or 1ug of rabbit IgG (ThermoFisher) antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... rabbit monoclonal antibody against Ki67 (Rabbit, RM-9106-S0; Thermo Fisher Scientific) and caspase-3 (Mouse ...
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bioRxiv - Plant Biology 2021Quote: ... Rabbit polyclonal anti-AtTET8 (Homemade) and normal rabbit immunoglobulin G (Thermo Fisher), were coated with protein A beads in IP buffer (20 mM MES hydrate ...
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bioRxiv - Immunology 2022Quote: ... or irrelevant rabbit IgG (anti-green fluorescence protein of rabbit IgG, ThermoFisher), or each of the mouse monoclonal or rabbit polyclonal CspZ-YA IgGs (0.4 nM ...