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Citations for Thermo Fisher :
1301 - 1350 of 10000+ citations for Tubulin gamma Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... washed three times in HBSS/1% BSA and incubated at 4 °C for 15 minutes in the dark with the following antibodies: LDLR (1/100; MAB CL 472413; Fisher scientific), CD36 (1/10 ...
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bioRxiv - Immunology 2022Quote: ... Fc and non-digested mAbs were removed from the flow-through by a 2 h incubation at RT with 200 µl of protein A resin per mg of initial mAb (Thermo Scientific). Finally ...
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bioRxiv - Microbiology 2022Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 μg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 μL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2019Quote: ... for the HC and LC of each mAb was transfected into 5ml of suspension Expi293 cells according to the manufacturer’s instructions (Thermo Fisher Scientific) in 50ml tubes ...
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bioRxiv - Biochemistry 2020Quote: ... primary CD4+ T cells were isolated from cones from anonymous donors with approval from the National Health Service Blood and Transplant (NHSBT) and stimulated with CD3 and CD28 mAbs on Dynabeads (Thermo Fisher) in the presence of 100 U/ml IL-2 to produce T cell blasts ...
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bioRxiv - Immunology 2020Quote: ... followed by staining with mAbs MM1a (anti-CD3) and ILA105 (anti-CD8) and secondary antibodies and Fixable Yellow Dead Cell Stain (Thermofisher Scientific). These cells were sorted using a FACSAria III (BD) ...
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bioRxiv - Immunology 2020Quote: ... the 5D5Δg Fab was produced by site-directed mutagenesis of the mAb 5D5 VH region using Accuprime Pfx Supermix (Thermo Fisher Scientific). 5D5Δg Fab was expressed in HEK293F cells and purified by chromatography as described above.
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Cryptococcus neoformans secretes small molecules that inhibit IL-1β inflammasome-dependent secretionbioRxiv - Immunology 2019Quote: ... cells were blocked for one h with a 10% FBS solution and stained for GXM with mAb 18B7 (10µg/mL) as a primary Ab and “Alexa Fluor® 633 Goat Anti-Mouse IgG” (Life Technologies) as a secondary Ab ...
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bioRxiv - Microbiology 2021Quote: ... Primary antibodies (anti-mouse nsP2 and E2 mAbs) were then added for 1h followed by incubation with Alexa Fluor (AF) 488-conjugated chicken anti-mouse antibody (Invitrogen, USA). Approximately 1×104 cells were acquired by the LSR-Fortessa flow cytometer (BD Biosciences ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Immunology 2021Quote: ... using HiTrap Protein A columns (Cytiva) for full length human and hamster mAbs and CaptureSelect CH1-XL MiniChrom columns (ThermoFisher Scientific) for Fab fragments ...
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bioRxiv - Microbiology 2020Quote: ... Cells were immunolabelled for 1 hour at RT with the indicated murine mAb and 45 min with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Life Technologies). Immunolabelled coverslips were counterstained with DAPI (Sigma) ...
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bioRxiv - Immunology 2022Quote: ... for 20 min before incubation overnight at 4°C in PBS-BR containing any of the following mAbs or reagents: DAPI nucleic acid (Molecular Probes), phalloidin Alexa fluor 488 (Molecular Probes) ...
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bioRxiv - Immunology 2023Quote: The 14.4.4 mAb producing hybridoma cell line (isotype: mouse IgG2a, kappa) was maintained at 37 °C and 5% CO2 in RPMI (Life technologies) supplemented with 5% FCS (Biowest) ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Microbiology 2023Quote: Splenocytes were stimulated with the peptide pools described in the memory phenotypic assay and supplemented with anti-CD28 and anti-CD49d mAbs (ThermoFisher Scientific) both at 1.0 μg/ml or a tissue culture medium with 1% DMSO as a negative control ...
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bioRxiv - Biochemistry 2023Quote: ... The anti-F or G mAbs were prepared under serum-free conditions using HyClone SFM4MAb (Thermo Fisher Scientific Inc., Rockford, IL) and purified by protein G-Sepharose affinity chromatography.
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bioRxiv - Immunology 2023Quote: ... plates were washed and incubated for one hour with SULFO-TAG conjugated anti-human IgG for mAbs (MSD) or SULFO-TAG conjugated polyclonal anti-human IgG+A+M (Thermo Fisher) for serum samples ...
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bioRxiv - Immunology 2023Quote: ... T cells were distributed into the round bottom 96 well plates at 3000 cells per well and stimulated by CD3/CD28 mAb-coated beads (ThermoFisher Scientific) at a ratio of 10:1 ...
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bioRxiv - Immunology 2023Quote: ... Non-specific staining was minimized by pre-incubating cells with 0.5 µg of Fc blocking mAbs (i.e., anti-CD16/CD32 antibodies, Invitrogen/eBioscience Thermo Fisher) in 100 µL of 1×PBS with 2% FCS for 15 min at 4 °C ...
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bioRxiv - Genomics 2023Quote: A mAb producing CHOK1 cell line (CHO K1 mAb) was seeded at a density of 2 × 105 cells/ml in 50ml SFM-II media (Gibco, 12052098) in 8 replicate shake flasks in a Kuhner orbital shaker at 170rpm at 5% CO2 ...
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bioRxiv - Immunology 2023Quote: Cryopreserved/thawed PBMCs were stained for 30 minutes at 4°C with the following reagents or conjugated anti-human mAbs: Live/Dead Aqua (Life Technologies), anti-CD4-PerCP (L200 ...
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bioRxiv - Microbiology 2023Quote: ... berghei infected HepG2 cells were immunolabeled using anti-PbHSP70 (1:200, 2E6 mouse mAb) (62)to mark EEFs followed by donkey anti-mouse Alexafluor488 (Invitrogen A21202). MSP1 was immunolabeled with Anti-PBANKA_083100 ...
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bioRxiv - Pathology 2024Quote: The binding affinity of Gremlin-1 or Gremlin-1-mAb mixtures to fluorescein-labelled Heparin (Heparin-FL, H7482, Thermo Fisher Scientific) was measured in a fluorescence polarization assay ...
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bioRxiv - Microbiology 2024Quote: ... Polprim and RPA -specific mAbs (approximately 100 µg of each ligand) were separately bound to 5mg of M-280 Tosyl activated Dynabeads (Invitrogen # 14203) O/N at 37⁰C on a rotating wheel ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used for these experiments: mouse anti-α-tubulin DM1α (1:500, Invitrogen 62204), rabbit anti-NuMA (1:400 ...
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bioRxiv - Cell Biology 2020Quote: ... An anti-tubulin antibody was used as a loading control (monoclonal mouse anti-a-Tubuline, Zymed, Thermofisher). The primary Rab7a antibody was purchased from Cell Signaling Technology (#2094S) ...
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bioRxiv - Cell Biology 2020Quote: ... MCAK and γ-tubulin were expressed and purified from Sf9 cells using Bac-to-Bac system (Invitrogen). The cells were lysed (EmulsiFlex ...
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bioRxiv - Cell Biology 2019Quote: Microtubules were polymerised from bovine tubulin with stochastic incorporation of ATTO390 (Attotech, Germany) and biotin (ThermoFisher, #20217) labelled tubulin ...
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bioRxiv - Cell Biology 2021Quote: ... Antibody dilutions (and suppliers) are as follows: alpha-tubulin DM1A 1:5000 (Thermo Fisher Scientific, Cat# MS581P1); TCF11/NRF1/NFE2L1 D5B10 1:1000 (Cell Signaling Technology ...
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bioRxiv - Cell Biology 2021Quote: ... The fraction of biotin-labeled tubulin was measured using a Biocytin Biotin Quantification Kit (Thermo Scientific #44610). Final stocks were diluted using unlabeled tubulin to 40 μM with a 33% biotin-labeled fraction.
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bioRxiv - Bioengineering 2021Quote: ... The alpha-tubulin goat antimouse antibody was revealed with Alexa Fluor 488 goat antimouse (Invitrogen, ThermoFisher Scientific). We rinsed Hela cells three times in PBS for 15 min ...
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bioRxiv - Bioengineering 2021Quote: ... The alpha-tubulin goat antimouse antibody was revealed with Alexa Fluor 488 goat antimouse (Invitrogen, ThermoFisher Scientific). We rinsed Hela cells three times in PBS for 15 min ...
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bioRxiv - Cell Biology 2020Quote: ... was purchased from ThermoFisher Scientific (Waltham, MA, cat# MA1-80017) and anti-a tubulin (1:10,000) was also purchased from ThermoFisher Scientific (clone B-5-1-2).
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bioRxiv - Cancer Biology 2019Quote: ... or with polyclonal antibody against β-tubulin (PA1-41331, 1:5,000; Thermo Fisher Scientific, Waltham, MA, USA) and the appropriate standard peroxidase-labeled anti-mouse IgG and anti-rabbit IgG secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... and soaked in a 1:500 dilution of primary antibody (rat anti-tubulin, Invitrogen Cat #MA1-80189) overnight at 4 °C ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Bioengineering 2023Quote: For LiveSR imaging: The tubulin-GFP plasmid was transfected into U2OS cells with Lipofectamine 3000 (Invitrogen, L3000) under the standard protocol.
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bioRxiv - Neuroscience 2023Quote: ... Cells were then stained using primary antibodies overnight at 4°C: tubulin (Invitrogen MA1-80017, 1:1000), aSyn (BD Transduction Laboratories 610786 ...
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bioRxiv - Developmental Biology 2023Quote: ... Antibodies used for immunofluorescence-labelling: FITC-conjugated mouse anti-α-tubulin (1:200, Alexa Fluor 488, Invitrogen). DNA was labelled using 10 min incubation in Hoechst 33342 (10 μg/ml ...
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bioRxiv - Microbiology 2020Quote: ... All PCR reactions were carried out using Recombinant Taq DNA Polymerase (5U/μl from Thermo Scientific) and standard PCR cycling conditions.
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bioRxiv - Cell Biology 2022Quote: ... Purified recombinant plasmid DNA was digested with PacI and transfected into HEK293 cells with Lipofectamine (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Recombinant antibodies were transiently transfected and produced in vitro in 293F Freestyle suspension cells (Thermofisher Scientific). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... and recombinant expression was undertaken using the FreeStyle™ MAX 293 Expression System (Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: ... iPSC line WTC-11 (Coriell Institute) was maintained on recombinant human vitronectin[36,37](Thermo Fisher Scientific), coating six-well tissue culture plastic plates (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2021Quote: ... The positive control consisted of 100 – 500 ng recombinant Protein A (ThermoFisher Scientific, Santa Clara, CA). The negative control consisted of ~2 μg wt-TMV from purified N ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant GST-SHP2 in supernatant was affinity-purified by Pierce glutathione agarose (Thermo Fisher Scientific) and eluted with lysis buffer containing 20 mM GSH ...
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bioRxiv - Zoology 2021Quote: ... The recombinant plasmid was quantified using a NANO-DROP 2000 spectrophotometer (Thermo Scientific, Waltham, MA, USA). The plasmid copy number was calculated using the equations described by Yun et ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human TNF-α a well established trigger of HIV-1 reactivation was purchased from Gibco. The PKC activator Bryostatin and Sodium Butyrate (NaBu ...