Labshake search
Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for TBARS MDA Universal Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2021Quote: ... 1 μg RNA was reverse transcribed using the TaqMan Reverse transcription kit and gene level detection performed using SYBR Green Master Mix (all Thermo Scientific). Relative expression was normalized to GAPDH and ACTB.
-
bioRxiv - Molecular Biology 2020Quote: RT-qPCR for LASV detection was performed using the Power SYBR Green RNA-to-Ct 1-step RT-qPCR kit (Thermo Fisher) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The microscope was adjusted and checked for the absence of chromatic shift during fluorochrome detection from different spectral regions using the TetraSpeck Fluorescent Microspheres Size Kit (ThermoFisher Scientific). Sequential scanning mode was used for each of the four channels (DAPI ...
-
bioRxiv - Microbiology 2022Quote: ... For LDH assays the supernatants of the cells treated with geneticin as described before were analysed with cytotoxicity detection kit (CyQUANT™ LDH Cytotoxicity Assay, Thermofisher) and the 100% was calculated with a well in which the supernatant contained 0.05% triton ...
-
bioRxiv - Developmental Biology 2019Quote: ... and TUNEL assay was performed with a Click-iT Plus TUNEL Assay for In situ Apoptosis Detection kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: ... Apoptosis was evaluated by Annexin V / Iodure Propidium staining using the Annexin V-FITC Apoptosis Detection Kit (Invitrogen™ eBioscience™).
-
bioRxiv - Developmental Biology 2019Quote: ... cydippids were fixed and processed for fluorescent detection of incorporated EdU using the Click-iT EdU labeling kit (Invitrogen Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2019Quote: ... Chemiluminescence detection was conducted with HRP-conjugated secondary antibodies and either SuperSignalTM West Dura or SuperSignalTM Femto kits (Pierce/Thermo Fisher Scientific ...
-
bioRxiv - Developmental Biology 2020Quote: ... The chemiluminescent detection of the horseradish peroxidase on the secondary antibodies was detected by the Supersignal West Dura kit (Thermo Scientific). Images and of western blots were acquired on a ChemiDoc MP system (Bio-Rad Laboratories ...
-
bioRxiv - Molecular Biology 2021Quote: ... membranes were washed three times 10 minutes in PBS-T and enhanced chemiluminescence detection was performed using the ECL Western Blotting substrate kit (Thermo Scientific). Quantifications were performed using Bio-Rad imaging software and graphs were generated using GraphPad prism ...
-
bioRxiv - Immunology 2020Quote: ... membranes were incubated with the appropriate HRP-conjugated secondary antibodies and developed using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific).
-
bioRxiv - Biochemistry 2020Quote: ... rocked for 3 hours after the third rinse before adding detection reagent (Pierce Fast Western Blot Kit, ECL substrate, Thermo Scientific). Blots were imaged using an AI 600 imager (GE Healthcare).
-
bioRxiv - Genomics 2021Quote: ... Following library validation and quantification (Agilent tape station, Peqlab KAPA Library Quantification Kit and the Applied Biosystems 7900HT Sequence Detection System), sequencing was performed on an Illumina NovaSeq 6000 instrument with 2×150 paired-end sequencing.
-
bioRxiv - Developmental Biology 2021Quote: ... EdU detection was carried out using the Click-iT EdU Alexa Fluor 488/555 imaging kit as per manufacturer instructions (Life Technologies).
-
bioRxiv - Immunology 2021Quote: ... Cells were stained with the Annexin V Detection Kit APC (eBioscience, Germany) and analyzed in an Attune flow cytometer (Invitrogen/ Thermo Fisher Scientific ...
-
bioRxiv - Genetics 2019Quote: ... and mutant detection was performed on adult legs with the Phire Animal Tissue Direct PCR kit (Thermo Fisher Scientific, Waltham, MA) with added LCGreen Plus+ Melting dye (Idaho Technology Inc. ...
-
bioRxiv - Cell Biology 2022Quote: ... EU incorporation and detection were performed using the Click-iT RNA Alexa Fluor 488 Imaging Kit (Thermo Fisher Scientific Ref. C10329). Oocytes were incubated in M2 + BSA ...
-
bioRxiv - Physiology 2022Quote: ... mRNA RTq-PCR was performed with the GeneAmp 7000 Sequence Detection System using the SYBR® Green reagent kit (Applied Biosystems)33 ...
-
bioRxiv - Molecular Biology 2022Quote: The temporal detection of CCs RNA transcription was performed by using Click-iT RNA Alexa Fluor 594 Imaging Kit (ThermoFisher Scientific), according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: Levels of GTP-bound Rac1 and Cdc42 were determined using either active Rac1/Cdc42 pull-down and detection kit (Cat# 16118/19, ThermoFisher Scientific) following the of the manufacturer’s instructions and buffer composition or purified GST-Pak1-PBD fusion beads ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Samples that were determined to be below the detection limit where quantified using <0.3 pg/mL sensitivity porcine TNF-α ELISA kit (Invitrogen, USA).
-
bioRxiv - Genetics 2023Quote: ... The membrane was incubated with streptavidin-conjugated horseradish peroxidase and then with reagents of the Chemiluminescent Nucleic Acid Detection Module Kit (ThermoFisher, 89880). The membrane was then scanned with the use of a ChemiDoc XRS+ System (Bio-Rad).
-
bioRxiv - Physiology 2023Quote: ... Samples were visualized using horseradish peroxidase coupled to appropriate secondary antibodies with enhancement by an ECL detection kit (Thermo Fisher Scientific).
-
bioRxiv - Cell Biology 2023Quote: ... the replication detection was carried out using the Click-iT EdU Cell Proliferation Kit for Imaging (AlexaFluor 555 dye; Invitrogen, USA) following the manufacturer’s protocol.
-
bioRxiv - Developmental Biology 2023Quote: EdU detection was conducted with (Click-iT™ EdU Cell Proliferation Kit for Imaging, Alexa Fluor™ 647 dye, Invitrogen, C10340) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Detection of EdU was accomplished using the Click-iT Plus EdU Alexa Fluor 488 or 647 Imaging Kit (Thermo Fisher Scientific) according to manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2023Quote: ... they were incubated for 10 min at RT with biotinylated polyvalent secondary antibodies (UltraVision Detection System HRP kit, Thermo Fisher Scientific). After three 5-min washes in PBS ...
-
bioRxiv - Genetics 2024Quote: ... 1 μg RNA was reverse transcribed using the TaqMan Reverse transcription kit and gene level detection performed using SYBR Green Master Mix (all Thermo Scientific). Relative expression was normalized to GAPDH and ACTB ...
-
bioRxiv - Developmental Biology 2024Quote: ... detection was performed on GOCs according to the Click-iT EdU Alexa Fluor 488 Imaging Kit manufacturer’s instructions (Molecular Probes, C10337). Briefly ...
-
bioRxiv - Cell Biology 2022Quote: ... the metabolic activity of viable cells was tested by a colorimetric dimethyl-thiazole-diphenyltetrazolium bromide (MTT) assay (cat. no. M6494, Invitrogen). Briefly ...
-
bioRxiv - Cancer Biology 2019Quote: ... Drug sensitivity of wild type cells were evaluated using a colorimetric cell proliferation assay (MTT assay, V13154, Thermo Fisher, MA) and a dose response curve was constructed by culturing cells and evaluating cell viability in the presence of various chemotherapy drug concentrations ...
-
bioRxiv - Cell Biology 2019Quote: ... The colorimetric reaction was then measured at 540 nm using a Multiskan Go microplate reader (Thermo Scientific, Rockford, IL, USA).
-
bioRxiv - Biochemistry 2022Quote: ... The dried peptides were resuspended in 200 mM EPPS pH 8.5 and quantified by bicinchoninic acid assay using the quantitative colorimetric peptide assay from Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2023Quote: ... Sep-Pak Vac tC18 solid phase extraction cartridges (Waters) and the concentration determined using the Pierce quantitative colorimetric peptide assay (ThermoFisher). Peptides were dried by vacuum centrifugation.
-
bioRxiv - Microbiology 2023Quote: ... qPCR was conducted in 384-well plates using the SensiFAST SYBR Lo-ROX Kit (Meridian Bioscience) on the QuantStudio 5 (384-well plate) qPCR cycler (Applied Biosystems, Foster City, CA, USA). The qPCR program encompassed 40 cycles ...
-
bioRxiv - Systems Biology 2021Quote: ... MDA-MB-231 human breast cancer cells were cultured in DMEM/F12 (1:1) media52 (Thermo Fisher Scientific, Life Technologies, Item #11330-032) supplemented with 10% Fetal Bovine Serum (Atlanta Biological ...
-
bioRxiv - Pharmacology and Toxicology 2019Quote: ... and MDA-MB468 (HTB-132) were obtained from the American Type Culture Collection (ATCC; Manassas, USA) and cultured in DMEM (Invitrogen Inc., Carlsbad, USA) with 2 mM L-glutamine and 80 μg/ml gentamycin ...
-
bioRxiv - Immunology 2020Quote: ... The plates were blocked with phosphate buffered saline (PBS) containing goat serum (2% v/v; Gibco, Grand Island, NY) and Tween-20 (0.1% v/v) ...
-
bioRxiv - Microbiology 2019Quote: Monoclonal antibodies (mAbs) were serially diluted 1:2 across white 96-well Nunclon© plates (Thermo Fisher Scientific, UK) in 50 μl of DMEM ...
-
bioRxiv - Cell Biology 2021Quote: ... Monocytes were then seeded on plastic at 2×106 cells/well in six-well plates in RPMI 1640 (Invitrogen) without FCS ...
-
bioRxiv - Systems Biology 2020Quote: ... We plated two thirds of the sorted cells onto 2-well glass bottom chamber plate (Nunc Lab-Tek 155380) for treating with vemurafenib (see below ...
-
bioRxiv - Immunology 2022Quote: ... The diluted sera were incubated for 2 hours at room temperature in 96 well plates (Maxisorp, Nunc, Roskilde, Denmark) coated with 100 ng/well of the RBD antigen ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were counted and plated in non-adherent 24 well plates in Neurobasal medium containing 2% B27 supplement (GIBCO), penicillin/streptomycin (Life technologies ...
-
bioRxiv - Immunology 2022Quote: ... The plate was coated with 2 µg/mL mouse anti-His antibody (Invitrogen cat. #MA1-21315-1MG, ThermoFisher Scientific) overnight at 4°C ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were plated at 1 x 10^6 cells per well of a 6 well plate or 5 x 10^6 cells per T25 flask in stage 2 media consisting of 47.5% IMDM (Gibco), 47.5% F12 (Gibco) ...
-
bioRxiv - Biochemistry 2020Quote: Transiently transfected C3H/10T1/2 cells in 12-well plates were washed twice with 1x PBS (Gibco, 14190-250) and collected by trypsinization 24 hours post-transfection for luciferase activity assays ...
-
bioRxiv - Immunology 2021Quote: ... The complex RBD:mAbs was then added to a pre-coated hACE2 (2 μg/ml in PBS) 96-well plate MaxiSorp (Nunc) and incubated 1 hour at room temperature ...
-
bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
-
bioRxiv - Cancer Biology 2022Quote: T98G cells (2 × 105 into 6 well plate) were reverse transfected with siRNAs (Table S3) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2020Quote: ... DEPC water—2 μl) was loaded in 96-Well Reaction Plates enclosed with microamp Optical Adhesive Film (Applied Biosystems). Standard cycling parameters and baseline thresholds were set manually along with UBI-3 as a reference gene ...