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Citations for Thermo Fisher :
1751 - 1800 of 10000+ citations for TBARS MDA Universal Colorimetric Detection Kit 2 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Annexin V and 7AAD staining was performed using eBioscience™ Annexin V Apoptosis Detection Kit eFluor™ 450 (Invitrogen, 88-8006-72). Briefly ...
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bioRxiv - Developmental Biology 2021Quote: ... Detection of EdU was performed using the Click-iT EdU Cell Proliferation kit with Alexa Fluor 647 dye (Thermo Fisher Scientific, C10340) in accordance with the manufacturer’s instructions.
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bioRxiv - Genomics 2019Quote: The total ATP concentration of frozen-thawed spermatozoa was analyzed using the ATP Determination Kit A22066 (Molecular Probes TM Invitrogen detection technologies) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2019Quote: The total ATP concentration of frozen-thawed spermatozoa was analyzed using the ATP Determination Kit A22066 (Molecular Probes TM Invitrogen detection technologies) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... All these steps are described in the protocol proposed by the manufacturer (Annexin V FITC Apoptosis Detection Kit, Invitrogen, Carlsbad, CA, USA). After 10 minutes ...
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bioRxiv - Cell Biology 2020Quote: Apoptotic cell death was measured by an Alexa Fluor® 488 annexin V and propidium iodide (PI) apoptosis detection kit (ThermoFisher Scientific) according to the manufacturer’s protocol ...
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Cwl0971, a novel peptidoglycan hydrolase, plays pleiotropic roles in Clostridioides difficile R20291bioRxiv - Microbiology 2021Quote: ... the supernatants from different strains were collected and filtered with 0.22 μm filters used for LDH concentration detection with the Pierce™ LDH Cytotoxicity Assay Kit (Thermo Fisher, USA) according to the instructions of the manufacturer ...
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bioRxiv - Cell Biology 2019Quote: ... Detection of RNA was performed in a two-step RT-qPCR using the High Capacity cDNA Reverse Transcription Kit (Applied Biosystems, # 4374966) and the SYBR green master mix ...
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bioRxiv - Molecular Biology 2019Quote: ... The quantity of DNA in each digest was measured by fluorescent signal detection using the Quant-iT™ dsDNA High-Sensitivity Assay Kit (Q33232, Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Permeabilization and EdU detection was carried out using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s manual ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative RT-PCR was performed using QuantiTect Probe RT-PCR Kit (Quiagen®) in an ABI PRISM 7500 Sequence Detection System (Applied Biosystems). Amplifications were carried out in 25 µL reaction mixtures containing 2× reaction mix buffer ...
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bioRxiv - Genomics 2024Quote: ... We then compared the performance of the different kits in terms of detection and quantification rates using standard RT-PCR (Applied Biosystems 7500 Fast Instrument, Thermo Fisher) and portable RT-PCR (Magnetic Induction Cycler quantitative PCR (Mic qPCR) ...
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bioRxiv - Molecular Biology 2024Quote: ... The hybridization signal from the biotin-labeled probe (listed in Supplementary Table 1) was detected using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Fisher Scientific), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... after which then genomic DNA was isolated for detection of gene editing efficiency using the GeneArt genomic cleavage assay kit (Invitrogen, catalog #A24372). Hep3Bcas9 cells had high cleavage efficiency and thus was chosen for generating knockout cell lines using CRISPR ...
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bioRxiv - Cancer Biology 2024Quote: ... qRT-PCR was performed using DyNAmo ColorFlash SYBR Green qPCR Kit in the ABI Quant Studio 5 Sequence Detection System (Life Technologies, USA). The expression of the genes of interest was analyzed by the ΔΔCt method using ATP5G rRNA and GAPDH as internal control genes ...
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bioRxiv - Immunology 2023Quote: ... Lysates were boiled for 5 minutes (except lysates used for detection of electron transport chain complexes) and protein content was determined using a BCA kit (23225, Thermo Fisher Scientific). Proteins were separated by SDS-PAGE and transferred to a 45 µm PVDF membrane ...
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bioRxiv - Cancer Biology 2023Quote: Fixed brain sections were rehydrated and underwent antigen retrieval before being processed for TUNEL assay using the Click-iT™ Plus TUNEL Alexa Fluor™ 647 Assay Kit for In Situ Detection (Invitrogen) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... The number of SIV copies was normalized to diploid genome cell equivalents co-determined by Q-RT-PCR using the TaqMan™ RNase P Detection Reagents Kit (Applied Biosystems), as described previously (11 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were counterstained using DAPI provided in the detection reagent kits and mounted in ProLong Gold mounting media (P36930, Thermo Fisher Scientific). The images were obtained using an Olympus FV3000 confocal laser scanning microscope with a 40X magnification oil immersion objective ...
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bioRxiv - Molecular Biology 2024Quote: TUNEL assays were performed on deparaffinized heart sections using a Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection (Invitrogen, C10617), according to the manufacturer’s instruction ...
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bioRxiv - Neuroscience 2024Quote: Single-molecule fluorescence in situ hybridization (smFISH) for miRNA detection in hippocampal neuron cultures was conducted using the QuantiGene ViewRNA miRNA Cell Assay Kit (Thermo Fisher, QVCM0001) following the manufacturer protocol with minor adjustments ...
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bioRxiv - Cell Biology 2024Quote: ... All cells were assessed for mycoplasma contamination at monthly intervals using the LookOut Mycoplasma Detection kit (MP0035, Thermo Fisher Scientific, Loughborough, UK).
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bioRxiv - Physiology 2024Quote: All real-time PCRs were carried out in 96-well plates using ChamQ SYBR qPCR Master Mix kit (Nanjing Vazyme Biotech Ltd., China) in the ABI Prism 7500 Sequence Detection System (Applied Biosystems, USA) at 95°C ...
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bioRxiv - Cell Biology 2021Quote: ... then resuspended in 10 μL 50 mM HEPES pH 8.5 and quantified by Pierce Quantitative Colorimetric Peptide Assay (Thermo Scientific 23275).
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bioRxiv - Cell Biology 2020Quote: ... followed with incubation at 37 °C for 1-hr before the colorimetric was read at 565 nm by a microplate photometer (Thermo Fisher). The iron level of each sample was normalized by the weight of skeletal muscles and presented as micrograms of iron per gram of wet tissue weight.
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bioRxiv - Immunology 2020Quote: ... The colorimetric reaction proceeded for 20 minutes at RT and was stopped by addition of 100 μl of H2SO4 1N (Fisher Scientific). The plates were then read within 30 minutes at 450 nm using a Synergy H1 microplate reader (Bio-Tek) ...
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bioRxiv - Cell Biology 2023Quote: ... Dried peptides were resuspended in DI water and then quantified using Pierce Quantitative Colorimetric Peptide Assay (Thermo Fisher Scientific, Rockford, USA).
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bioRxiv - Plant Biology 2023Quote: ... The concentration of free glucose released from crystalline CMFs from each sample was measured by colorimetric assay using a NanoDrop 2000c spectrometer (Thermo Scientific), and then cellulose content was calculated as the protocol suggests ...
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bioRxiv - Molecular Biology 2024Quote: ... and whole tissue lysates from the other studies were quantified using bicinchoninic acid (BCA) colorimetric assays (Thermo Scientific, Waltham, MA, USA). Isolates and lysates from all studies were then prepared for Western blot analysis with 4× Laemmli buffer for final concentration preparations at 1 μg/μL ...
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bioRxiv - Molecular Biology 2023Quote: ... Membrane development was performed by an enhanced chemiluminescence-based detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare, Fisher Scientific) using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Molecular Biology 2023Quote: ... Membrane development was performed by an enhanced chemiluminescence-based detection method (ECL™ Prime Western Blotting Detection Reagent, GE Healthcare, Fisher Scientific) using a ChemiDoc MP system (Bio-Rad) ...
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bioRxiv - Bioengineering 2023Quote: Human breast cancer cell lines, including MCF7, MCF7/GFP, and MDA-MB-231/GFP (ATCC, Manassas, VA) were cultured in DMEM medium (Thermo Fisher Scientific, Waltham, MA) supplemented with 10% (v/v ...
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bioRxiv - Bioengineering 2023Quote: MDA-MB-231 cells were plated in an 8-well chamber (1 × 104 cells/well) (Nunc™ Lab-Tek™ II chambered cover glass) and cultured for three days ...
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bioRxiv - Plant Biology 2020Quote: ... Four-day-old seedlings grown under constant white light (70 μmol s−1 m−2) were transferred to 96-well plates (136101 Nunc MicroWell White plate ...
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bioRxiv - Plant Biology 2020Quote: ... After 24 h illumination cells were resuspended and re-plated onto f/2-NH4 agar plates containing 80 μg mL−1 zeocin (Invitrogen). The plates were maintained under illumination for 3 weeks and the resistant colonies were screened by PCR for the presence of SYN genes as previously described (Falciatore et al. ...
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bioRxiv - Cell Biology 2020Quote: ... or on YPD plates (1% yeast extract, 1% peptone and 1% glucose) containing 2% agar supplemented with 100 µg/mL zeocin (Invitrogen), 300 µg/mL hygromycin B (Invitrogen ...
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bioRxiv - Genetics 2021Quote: Live myofibers in the 6-well plate were stained with 2 µL of 5mg/mL of Hoechst (Molecular Probes, H1399) in 2 mL of 1X PBS for 5 minutes in a 37 °C with 5% CO2 incubator ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell-conditioned medium was collected grown for 3 days in cell culture flasks (WaGa cells) or 150 mm plates (MCC26, pCDNA and Flag-circALTO1/2 transfected 293T cells) with exosome-depleted FBS (Invitrogen). The collected media was first centrifuged at 300×g for 10 min at room temperature to pellet and remove cells ...
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bioRxiv - Immunology 2021Quote: ... Individual B cells were sorted into 96-wells PCR plates (Bioké) containing 2 μL lysis buffer (0.2% (v/v) Triton X-100 + 2U/μL RNaseOUT™ Recombinant Ribonuclease (Invitrogen) in UltraPure™ DNAse/RNase free distilled water (Invitrogen) ...
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bioRxiv - Bioengineering 2019Quote: ... plasmids (100 ng) were transfected into cells as duplicates (2 × 104/well in a 96-well plate) with Lipofectamine 3000 (Invitrogen) in Opti-MEM (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... proliferating NSCs were plated on matrigel-coated 6- or 12-well plates and cultured in Neurobasal medium supplemented with B27 without vitamin A (2%, ThermoFisher), PenStrep (1% ...
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bioRxiv - Microbiology 2019Quote: ... were transfected into BV-2 cells or NA cells (106 cells/well) in 12-well plates using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... and peptide-E3 ligase fusion (250 ng) plasmids were transfected into cells (2 × 105/well in a 24-well plate) with Lipofectamine 3000 (Invitrogen) in Opti-MEM (Gibco) ...
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bioRxiv - Bioengineering 2020Quote: ... were seeded on 24-well plates and transfected 16 hours later at 60-80% confluency with 2 ul Lipofectamine 2000 (ThermoFisher), 1.5 ug pCMV-PE2 plasmid (Addgene #132775) ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Cancer Biology 2020Quote: Cells were sorted into 96-well plates at 50 cells/well in 5 µL CellsDirect™ 2× Reaction Buffer (Invitrogen). Cells lysates were reverse transcribed using Superscript™ III (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: T47D cells were seeded in six-well plates (50 000 cells/well) in 2 ml of cell medium (10% hiFBS [Fisher] and 1% Pen/Strep [Invitrogen]). On Day 1 ...
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bioRxiv - Microbiology 2020Quote: Vero or A549-hACE2 cells (5,000 cells per well in phenol-red free medium containing 2% FBS) were plated into a clear flat bottom 96-well plate (Nunc). On the next day ...
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bioRxiv - Immunology 2019Quote: Total splenocytes were cultured into 96 well tissue culture plates in triplicate at 2 x 105 cells in RPMI 1640 (Gibco) supplemented with 1M NEAA ...
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bioRxiv - Neuroscience 2020Quote: ... were premixed and incubated at room temperature for 25 min in the following amounts per plate: 2 mL opti-MEM (GIBCO), 3 μg pRTR2 ...