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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Recombinant Mouse Fcgr4 His & AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Immunology 2022Quote: Recombinant proteins were produced using the Freestyle™ 293 Expression System (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were treated with a Kv1.1 recombinant rabbit monoclonal antibody (SN66-06, ThermoFisher Scientific) overnight and with an Alexa-488 conjugated secondary antibody for two hours on the following day ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 5 μg/ml gibco recombinant AOF insulin (cat. nr.: A11382II, ThermoFisher Scientific, Massachusetts, USA) and 50 μM hydrocortisone hemisuccinate (cat ...
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bioRxiv - Molecular Biology 2022Quote: ... All the recombinant proteins were transformed and expressed in E.coli BL21 (DE3) (Invitrogen, USA).
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bioRxiv - Immunology 2022Quote: ... A recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculovirus was generated by transfecting purified bacmid DNA into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Cell Biology 2022Quote: ... 4h prior treatment with recombinant human epidermal growth factor (EGF) (Cat# RP-10927, Invitrogen) at 5 ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant baculoviruses were recovered using the Bac-to-Bac bac-ulovirus expression system (Invitrogen). N WT or N1-370 bacmids were obtained after transforma-tion of DH10EMBacY bacteria (Geneva Biotech) ...
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bioRxiv - Microbiology 2023Quote: ... Recombinant BA.5 and BQ.1.1 RBD proteins were produced in Expi293F cells (ThermoFisher) by transfection of DNA using the ExpiFectamine 293 Transfection Kit (ThermoFisher) ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 647-conjugated recombinant human EGF (Invitrogen, #35351, Lot# 1700382, 1:1000) to label EGFR.
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bioRxiv - Immunology 2023Quote: ... supplemented with 294.4 ng/l human recombinant Epidermal Growth Factor (EGF) (Gibco, 10450-013), 25 mg/l Bovine Pituitary Extract (Gibco ...
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were stabilized by adding the RNAseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific), before freezing at -80°C until ready for further analysis.
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bioRxiv - Plant Biology 2023Quote: ... RNA extracts were stabilized by adding the RNAseOUT recombinant ribonuclease inhibitor (Thermo Fisher Scientific), before freezing at −80°C until further analysis.
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bioRxiv - Biophysics 2023Quote: Recombinant baculovirus was generated by transfecting Sf9 cells with bacmid using Cellfectin II (ThermoFisher). Baculoviruses were harvested from Sf9 cell media by filtering through a 0.22 µm filter and amplified three times before using for cell transduction ...
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bioRxiv - Biochemistry 2022Quote: ... Recombinant protein was resolved by NuPAGE™ 4-12% Bis-Tris Gel (Invitrogen; NP0322BOX) to determine identity and purity ...
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bioRxiv - Biochemistry 2023Quote: The following recombinant proteins were purchased from commercial sources: alkaline phosphatase (Thermo Scientific #EF0651), Antarctic phosphatase (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: ... the epidermis was separated from the dermis and incubated in TrypLETM recombinant enzyme (ThermoFisher) for 1h and subsequently dissociated mechanically with a pipette tip ...
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bioRxiv - Immunology 2023Quote: ... and recombinant Cas9 protein via electroporation using the Neon transfection system (Thermo Fisher Scientific). The following day ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was generated using the Bac-to-Bac system (Life Technologies). Baculoviruses were generated by transfecting purified bacmid DNAs into Sf9 cells using FuGENE HD (Promega) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant proteins were purified using HisPur™ Ni-NTA resin (Thermo Fisher Scientific) and amylose resin (NEB) ...
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bioRxiv - Developmental Biology 2023Quote: ... Recombinant anti-GFP nanobody was conjugated to epoxy-activated magnetic Dynabeads M270 (Life Technologies) using 10 μg recombinant protein/1 mg Dynabeads ...
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bioRxiv - Microbiology 2023Quote: ... and STRN3 were carried out utilising immobilised recombinant protein A/G resin (Thermo Scientific). Cell pellets were harvested and processed as described in the EGFP co-immunoprecipitation section ...
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bioRxiv - Neuroscience 2022Quote: ... An injection of Cholera Toxin Subunit B (recombinant) conjugated to Alexa Fluor 647 (ThermoFisher) was made in the striatum of the same hemisphere (+1.2mm AP ...
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bioRxiv - Cell Biology 2023Quote: Recombinant tau monomers (human 4R2N T40/ Evotec, Germany) were labeled with Cy3 (Life Technologies). To prepare tau oligomers ...
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bioRxiv - Immunology 2023Quote: ... Recombinant antigen was immobilized on N-hydroxysuccinimide (NHS)–activated agarose resins (Thermo Fisher, 26197) by overnight rotation at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained with anti-CD64 (CD64 Recombinant Rabbit Monoclonal Antibody, Thermofisher, # MA5-29706) at a dilution of 1:400 in 1% BSA in PBS and incubated at 4°C overnight in the dark overnight ...
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bioRxiv - Immunology 2023Quote: ... recombinant bacmid DNA was generated using the Bac-to-Bac system (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Recombinant RBD (Arg319 – Phe541) of the same strain was obtained from Invitrogen (# RP 87678) and used as an immunogen without conjugation with KLH and used for screening assays ...
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bioRxiv - Biophysics 2021Quote: ... Control Drosophila melanogaster biotin-tagged kinesin-1 motors were labeled with streptavidin-conjugated Qdot 655 (Life Technologies, Carlsbad, CA) at a 1:4 motor:Qdot ratio as previously described [16 ...
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bioRxiv - Cell Biology 2020Quote: ... K16-flag protein (C-terminally tagged) was made using the pcDNA™3.1 Directional TOPO™ Expression Kit (ThermoFisher) using primers for the mouse Krt16 gene with flag peptide sequence on the 3’ end (Supplemental Table 1).
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bioRxiv - Cell Biology 2020Quote: ... The pDONR201 clones were transferred into GFP or SFB tagged DEST vectors using the Gateway LR cloning system (Invitrogen). KDM5AΔPID deletion mutant was generated by PCR in pDONR201 vector following standard cloning method and then subcloned into Gateway destination vector derived from pcDNA5/FRT/TO plasmids containing the NLS and SFB- or GFP-tagged in N-terminal ...
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bioRxiv - Cell Biology 2020Quote: ... GFP or luciferase tagged versions of the proteins were generated using the Gateway recombination cloning procedure (Invitrogen, Carlsbad, CA) and custom-made vectors.
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bioRxiv - Molecular Biology 2021Quote: GPI-APs were HA-tagged by cloning their coding cDNA amplified from the human cDNA library or from Invitrogen plasmids ...
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids with 3xFLAG tagged SARS-CoV-2 proteins were transiently expressed in BEAS-2B cells using Lipofectamine 3000 (ThermoFisher) transfection according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2020Quote: HEK293T cells grown on 100-mm dishes were cotransfected with Flag-tagged ASK1_KR and PKA or empty vector using Lipofectamine 2000 (Invitrogen). Twenty-four hours after transfection ...
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bioRxiv - Cell Biology 2022Quote: ... Transferrin (Tf) and Epidermal Growth Factor (EGF) tagged with Alexa Fluor 488 was obtained from Invitrogen (T13342 and E13345). Dako fluorescent mounting medium from Agilent (S3023 ...
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bioRxiv - Cell Biology 2019Quote: ... FLAG-tagged human β2AR with S204/207A double mutations (FLAG-mutant) was generated by Gibson assembly method (Thermo Fisher) using FLAG-β2AR and synthetic gBlocks with the double mutations as templates (Integrated DNA Technologies) ...
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bioRxiv - Genomics 2020Quote: ... 20.25 μg 3×Flag-tagged dCas9 plasmid and 20.25 μg sgRNA plasmid were transfected into cells using Lipofectamine 3000 (Invitrogen). Two biological replicates were performed for each condition ...
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bioRxiv - Cancer Biology 2021Quote: Control MCF-10A cells or cells expressing HA-tagged PEAK3 were cultured in -EGF medium (DMEM/F-12 (Gibco) supplemented with 0.5◻μg/ml hydrocortisone (Sigma) ...
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bioRxiv - Immunology 2021Quote: Purified human MYC- or Fc-tagged NKp46 were labeled with Alexa647 using the microscale protein labeling kit (ThermoFisher Scientific). Alexa647-labeled NKp46-Myc (50 nM ...
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bioRxiv - Biophysics 2021Quote: Full-length HA-tagged p75NTR and full-length Myc-tag TRADD were expressed from a pcDNA3 vector backbone (Invitrogen). The HA-p75E345A ...
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bioRxiv - Biochemistry 2021Quote: N-terminal GST-tagged MBNL1 (amino acids 1-260)59 was recombinantly expressed using BL21 Star (DE3) cells (Invitrogen) and protein expression induced using 0.5 mM IPTG at an OD600 = 0.6-0.7 for 2 hours at 37°C ...
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bioRxiv - Biophysics 2021Quote: ... and the tagged SΔTM was purified by affinity chromatography using Ni-NTA agarose beads (Invitrogen™, Waltham, MA, USA) and size exclusion chromatography as described above.
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bioRxiv - Microbiology 2021Quote: ... bacteria were tagged by Gfp or stained by cell membrane stain FM4-64 (10 µM final concentration, Molecular Probes) for 2 mins in the dark (without flow) ...
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bioRxiv - Cell Biology 2021Quote: ... Stable cell lines expressing BioID tagged genes of interest were generated by transfection with Lipofectamine 2000 (Thermo Fisher Scientific) and a combination of the appropriate pcDNA5/FRT/TO construct and Flipase expressing pOG44 plasmid ...
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bioRxiv - Cell Biology 2021Quote: ... Plasmids for expressing tagged proteins in S2 cells were generated by cloning coding sequences into pEntr/SD (Thermofisher, K242020), and recombining the coding sequences into pAc5 promoter destination vectors (pAWM and pAWF) ...
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bioRxiv - Cell Biology 2021Quote: ... and GST-tagged baits contained in supernatants were normalized using Pierce Glutathione Magnetic Agarose Beads (Thermo Fisher Scientific, #78602) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: IPs with FLAG-tagged protein were performed with Protein G magnetic beads (Invitrogen™ Dynabeads™ Protein G; #10004D) and α-FLAG antibody (Monoclonal ANTI-FLAG® M2 antibody produced in mouse ...
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bioRxiv - Cell Biology 2022Quote: ... Cells at 40% confluence were transfected with 1 ug of GFP-tagged LRRK2 plasmid using Lipofectamine 2000 (Invitrogen, 11668019) as described for 12-15 hrs ...
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bioRxiv - Neuroscience 2019Quote: ... Myc-tagged proteins were immunoprecipitated using anti-c-Myc monoclonal antibody supernatant (9E10, DSHB) and protein A-agarose (Invitrogen). Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma) ...