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Citations for Thermo Fisher :
1401 - 1450 of 10000+ citations for Recombinant Mouse Fcgr4 His & AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... at a 1:1 ratio and recombinant human IL-2 (Peprotech, ThermoFisher Scientific), and expanded in RPMI 1640 medium supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Immunology 2024Quote: Recombinant proteins were coated on Immulon 4 HBX 96-well plates (Thermo Scientific) at a concentration of 2 μg/mL in PBS ...
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bioRxiv - Molecular Biology 2020Quote: ... these PER2 cDNAs were sub-cloned into various tagged vectors including pcDNA3.0.HA (Thermo Fisher Scientific, Waltham, MA), 3XFLAG-CMV-7.1-2 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... FAM fluorescent tagged TaqMan® copy number assay probes detecting the ETNK1 gene (Hs01860263_cn) (Life Technologies, Carlsbad, CA) was used to quantify the copy number of 12p ...
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bioRxiv - Cell Biology 2020Quote: Overexpression of mCherry-tagged actin constructs in HEK293T cells was carried out using Lipofectamine 2000 transfection reagent (ThermoFisher) at a ratio of 1:2.5 ...
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bioRxiv - Bioengineering 2021Quote: ... The Arabidopsis eEF-1 α gene (Assay ID. No. At02337969_g1) tagged with VIC dye (Life Technologies, Carlsbad, CA) was added in each well as an internal control ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged TFs assayed in PBMs were detected either with Alexa488-conjugated anti-GST antibody (Invitrogen A-11131), or with a TF-specific PCRP antibody ...
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bioRxiv - Biophysics 2021Quote: ... 0.2 µg DNA encoding GFP-tagged T-Plastin (WT or mutant) and 0.25 µL Lipofectamine2000 (Thermo Fisher Scientific), mixed in 20 µL OptiMEM (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The expressed C-terminal 6xHis-tagged Nluc was Ni-NTA purified using HisPurTMNi-NTA Resin (ThermoFisher scientific, #88221) as per the HisPurTMNi-NTA Resin protocol ...
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bioRxiv - Cell Biology 2021Quote: ... AF555-and AF647-tagged secondary antibodies (donkey; 1:1000, cat. num. A-21208, A-31572, A-31571, ThermoFisher).
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bioRxiv - Cell Biology 2022Quote: 6-dpf zebrafish larvae were gavaged with Alexa Fluor 488-tagged dextran 10,000MW (fDex) solution (Molecular Probes, USA) or purified mCherry solution (prepared in the laboratory ...
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bioRxiv - Molecular Biology 2022Quote: MCP-tagged SARS-CoV-2 expression plasmids for the MS2 tethering assay were generated by Gateway Assembly (ThermoFisher). SARS-CoV-2 ORFs were amplified by PCR (KAPA HiFi HotStart ReadyMix ...
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bioRxiv - Biochemistry 2019Quote: ... anti V5-HRP conjugate (to detect mSCARLET and other V5 tagged fluorescent proteins, catalog number 46-0708, Invitrogen), rabbit polyclonal anti TAL1 IgG (catalog number A305-300A ...
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bioRxiv - Neuroscience 2019Quote: FLAG-tagged human LRRK2 proteins were enriched from injected rat striatum using Protein G-Dynabeads (50 µl; Invitrogen) pre-coupled with mouse anti-FLAG-M2 antibody (5 µg ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Biochemistry 2021Quote: Flag-tagged UCH37 (WT, C88A, ΔCTD, ΔCTD/C88A, EWI, EWI/C88A) were cloned into pcDNA5/FRT/TO (Invitrogen). Stable cell lines that inducibly express these proteins were constructed from Flp-InTM T-RExTM 293 Cells following manufacturer’s instructions.
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bioRxiv - Developmental Biology 2020Quote: ... Cos-7 cells were transfected with 2.5μg of hexahistidine (His6) tagged TUBA1A plasmid DNA using Lipofectamine 3000 (Invitrogen). For Cos-7 proteasome inhibition assays ...
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bioRxiv - Cell Biology 2021Quote: ... 10 μg of GST tagged protein was conjugated to Pierce™ Glutathione Magnetic Agarose Beads (Thermo Fisher, 78602), for 1hr at RT ...
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bioRxiv - Biochemistry 2021Quote: ... 10 mM maltose.) SKD3-MBP fusion protein was cleaved with a 6His-tagged AcTEV™ protease (Thermo Fisher) at 4 °C for 96 h in the amylose elution buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Inserts were then subcloned into pCS2+ N’ HA tagged vectors that had been converted into Gateway® (Invitrogen) destination vectors ...
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bioRxiv - Molecular Biology 2020Quote: ... at a 1:5000 dilution and endogenous LC3 was tagged with an LC3B/MAP1LC3B primary antibody (ThermoFisher, L10382) used at a dilution of 1:500 for 72 hours at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... stably expressing N-terminally GFP-tagged murine GBP2 (GFP-mGBP2) or GFP alone11 were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen/Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... and the HA-glmS-tagged parasite lines were coated on diagnostic slides (Epredia; Thermo Fisher Scientific; Waltham, US) and air-dried ...
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bioRxiv - Molecular Biology 2022Quote: ... GST-tagged bait proteins were immobilized in a 96-well Flat bottom Nunc MaxiSorp plates (Nunc, Roskilde, Denmark) at 4°C for 18 hours (10 μg per protein in 100 μl PBS) ...
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bioRxiv - Molecular Biology 2023Quote: ... Human PKCβII was YFP-tagged at the N-terminus in a pcDNA3 vector using Gateway Cloning (Life Technologies) as described in (6) ...
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bioRxiv - Biochemistry 2022Quote: Cloning of full-length N-terminal strepII-tagged hItln1 into the pcDNA4/myc-HisA vector backbone (Life Technologies) was described previously (14) ...
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bioRxiv - Cell Biology 2023Quote: ... To visualise the cell surface with Alexa488-tagged wheat germ agglutin (Thermo Fisher Scientific, UK, 1:2000 dilution) an incubation was done prior to permeabilization ...
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bioRxiv - Molecular Biology 2023Quote: ... Genes encoding tagged and untagged SOSIP.664 Env homotrimers were expressed by transient transfection of Expi293 cells (ThermoFisher). Env heterotrimers were purified from co-transfections involving a 20:1 expression plasmid DNA ratio of untagged to tagged Env constructs ...
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bioRxiv - Cell Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5′ ends were synthesized by Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: FLAG-tagged β-catenin variants were expressed in transient transfection assays using the pCDNA3.1 vector (Thermo Fisher Scientific). A plasmid expressing human β-catenin containing a S33Y mutation (pCDNA3-S33Y ...
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bioRxiv - Molecular Biology 2023Quote: ... Readout probes tagged with Alexa 488 and Alexa 647 at their 5’ ends were synthesized by Thermo Scientific, while those labeled with ATTO565 at the 5’ end was synthesized by Sigma Aldrich ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Chromatin immunoprecipitation of FLAG-tagged TnpB and TldR proteins was performed using Dynabeads Protein G (Thermo Fisher Scientific) slurry (hereafter ...
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bioRxiv - Bioengineering 2024Quote: GFP-HUVECs were added to well 1 and GFP-HUVECs co-tagged with Hoechst (NucBlue Live ReadyProbes, Invitrogen) were added to wells 2-4 ...
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bioRxiv - Cell Biology 2023Quote: ... strep tagged APC/C was pre-incubated on ice with streptavidin conjugated Alexa Fluor 700 (ThermoFisher Scientific S21383) for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... cells were either transduced to include a fluorescent reporter or fluorescently tagged using Hoechst (NucBlue Live ReadyProbes, Invitrogen) or general membrane cell tracker (Cellvue Claret Far Red ...
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bioRxiv - Evolutionary Biology 2023Quote: Lamprey embryos were injected with MYC-tagged lamprey (pou5) or Xenopus (pou5f3.1, pou5f3.2, pou5f3.3) mRNA and a FITC dextran tracer (ThermoFisher AAJ6360622) into one blastomere at the two-cell stage at the same concentration used for injections in Xenopus ...
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bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
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bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Biochemistry 2020Quote: ... optiMEM (1X) reduced serum medium and heat-inactivated fetal bovine serum (HI-FBS) were from Gibco chemicals ...
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bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
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bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
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An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using a pcDNA 3.1-V5/His TOPO-TA cloning kit (Invitrogen K480001) per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...
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bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
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bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
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bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.