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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: HEK293 or HeLa cells were co-transfected with pBABED and pX335 plasmids using Lipofectamine 2000 (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: Human Embryonic Kidney 293 (HEK293) cells (ATCC CRL-1573) were cultured in complete growth Dulbecco’s modified Eagle’s medium (DMEM, Gibco), supplemented with 10% fetal bovine serum (UCSF Cell Culture Facility) ...
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bioRxiv - Microbiology 2021Quote: HeLa cells (ATCC CCL-2) and HEK293 cells (ATCC CRL-1573) were maintained in DMEM high glucose (Gibco) containing 10% heat-inactivated fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The recombinant constructs were transfected into HEK293 cells either individually or together using Lipofectamine® 3000 reagent (Invitrogen) following the instructions provided ...
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bioRxiv - Systems Biology 2022Quote: ... HEK293 cells were prepared as 2 × 105 cells/mL in DMEM (Dulbecco s Modified Eagle Media, Life Technologies) + 10% FBS (Foetal Bovine Serum ...
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bioRxiv - Biophysics 2022Quote: ... The Fab version of the antibody fusion (Fab-ShK) was expressed in freestyle HEK293 (293F) cells (Thermo Scientific) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Human embryonic kidney cells (HEK293) were cultured in culture flask (150 cm2) to 80 % confluence in Dulbecco’s modified Eagle’s medium (DMEM) (Gibco) supplemented with 50 units/ml penicillin G (Gibco) ...
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bioRxiv - Biochemistry 2020Quote: ... HELA cells (DSMZ ACC-57) and HEK293 cells (DSMZ ACC-305) were cultured in DMEM (Thermo Fisher Scientific) supplemented with 10% FCS ...
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bioRxiv - Molecular Biology 2020Quote: ... and renilla luciferase plasmid (1 ng) were transfected into HEK293 cells or HCT116 cells by lipofectamine2000 (Invitrogen, 11668030) following manufacturer’s manual ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293-T cells were seeded into 10 cm plates with DMEM containing 0.5 mg/mL geneticin G418 (Thermofisher). Once the cells reached approximately 80% confluence ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 FcγRII cells (a gift from Dr. Craig Roy) were cultured in Dulbecco’s Modified Eagle Medium (DMEM; Gibco) supplemented with 10% heat-inactivated fetal bovine serum (HIFBS ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293 Flp-In T-Rex cells were co-transfected with pOG44 Flp-Recombinase Expression Vector (Invitrogen™, V600520), and pcDNA5 FRT TO plasmids expressing appropriate miniTurbo gene fusions ...
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bioRxiv - Neuroscience 2019Quote: HEK293 and SH-SY5Y cells (ATCC) were cultured in phenol red-free Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 2 mM L-Glutamine (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: HEK293 cells were transfected with EBV-BAC DNA using Lipofectamine 2000 reagent (Thermo Fisher Scientific, Waltham, MA, USA) followed by hygromycin selection ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA knockdown of AC1 and AC9 expression in HEK293 cells was carried out using Lipofectamine RNAiMAX (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The N-terminal 8xHis-TwinStrep-GFP-tagged NKCC1 constructs were expressed in GripTite HEK293 MSR cells (Thermo Fisher) in 24 well plates ...
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bioRxiv - Cell Biology 2020Quote: ... HEK293 Flp-In™ T-REx™ cell lines were generated according to the manufacturer’s instructions (ThermoFisher Scientific). BirA-containing plasmids (0.4 µg) ...
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bioRxiv - Genomics 2022Quote: Adherent HEK293 cell lines were grown in 10 mLs of DMEM (ATCC 302002) containing 10% FBS (Gibco 16000044) and 1% Pen/Strep (Gibco 15070063) ...
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bioRxiv - Microbiology 2022Quote: ... as well as the HEK293 cell line were grown in Dulbecco’s Modified Eagle’s Medium F-12 50/50 (DMEM/F-12, GIBCO) supplemented with 10% FBS at 37°C with 5% CO2 in a humidified chamber ...
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bioRxiv - Molecular Biology 2023Quote: ... Lentivirus production was performed according to the manufacturer’s recommendations in HEK293 E cells using Lipofectamine 3000 (ThermoFisher Scientific). Virus-containing supernatant was collected after 24 and 48 h and combined ...
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bioRxiv - Immunology 2023Quote: HEK293 cells (ATCC CRL-1573) were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Paneco) supplemented with glutamine (Gibco), 10% fetal bovine serum (HyClone) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Stable HEK293 cell lines were achieved by antibiotic selection with G-418 (1mg/ml, Gibco-BRL, Paisley, UK) added to the medium 3 days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK293 cells were cultured in a 2L suspension using FreeStyle 293 Expression Medium (Life Technologies, Carlsbad, CA, USA) with a cell density maintained at 1 × 10^6 cells/mL on the day of transfection ...
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bioRxiv - Microbiology 2024Quote: ... HEK293 ACE2 and lung carcinoma epithelial cells A549 ACE2 were cultivated with Dulbecco’s Modified Eagle Medium (DMEM; Gibco) with 10% fetal bovine serum (Sigma-Alrich ...
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bioRxiv - Bioengineering 2023Quote: ... which then were co-transfected into HEK293 6E cells for expression together following standard protocols for 293Fectin (Invitrogen). The cell cultures were harvested at 5 d post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: HEK293-6E cells (used for protein expression) were cultured in expression medium (FreeStyle F17 (Gibco, Cat#A13835-01)) supplemented with 1% FBS (Sigma ...
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bioRxiv - Cell Biology 2023Quote: Hek293T and Hek293 Flp-In T-REx cells were maintained without antibiotics in Dulbecco’s modified Eagle’s medium (DMEM) (Invitrogen) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293 cells were transiently transfected with 1 µg of each cDNA using Lipofectamine 2000 (Invitrogen, Carlsbad CA, USA). After 12 h of transfection ...
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bioRxiv - Plant Biology 2023Quote: ... HEK293 cells were seeded and grown on poly-lysine-coated Lab-Tek II eight-well chamber slides (NUNC) overnight and transfected with plasmid DNA using the Lipofectamine™ 3000 Transfection System (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... HEK293 cells and MCF7 cells purchased from ATCC were cultured in DMEM (high glucose, Life Technologies Corporation, 11965084) containing 15% fetal bovine serum ...
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bioRxiv - Genomics 2023Quote: ... sgRNA-transduced HEK293-idCas9 cells were cultured in high glucose DMEM medium supplemented with L-Glutamine (Gibco 11965092) and 10% FBS ...
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bioRxiv - Biochemistry 2022Quote: Lentivirus was made by transfecting HEK293 cells with ViraPower packaging plasmids (pLP1, pLP2, and pLP/VSVG) from Invitrogen along with the viral vector using JetPrime ...
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bioRxiv - Cell Biology 2023Quote: Human embryonic kidney (HEK293) cells were obtained from ATCC and cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: Approximately 1 × 105 HEK293 cells were seeded in flat-bottom 24-well plates (Thermo Fisher Scientific or FALCON) and incubated overnight ...
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bioRxiv - Immunology 2023Quote: ... Plasmids were then transfected into human embryonic kidney cells (HEK293-E, National Research Council, Canada) using 293Fectin (Thermofisher). Six days post-transfection ...
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bioRxiv - Neuroscience 2023Quote: ... HEK293S GnTI− cells (ATCC CRL-3022) were grown in suspension at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Neuroscience 2023Quote: Human embryonic kidney (HEK293) cells obtained from ATCC (Manassas, VA, USA) were cultured in Dulbecco’s Modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: HEK293 were also cultured in Dulbecco’s Modified Eagle Medium 1X (41966-029) with 10% FBS (GIBCO, 10270-106) and 1% PIS in a 10 cm dish and maintained in an incubator at 37°C and 5% CO2 ...
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bioRxiv - Biophysics 2024Quote: ... the HEK293 cells were incubated with 1 μM Alexa Fluor-647 conjugated α-bungarotoxin (BTX-647; Life Technologies) at 4°C for 1 hr ...
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bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid was transfected into HEK293 cells at 70% confluency using Genejuice (Fisher Scientific 70-967-3) at a 1:3 ratio (DNA ...
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Recruitment of the m6A/Am demethylase FTO to target RNAs by the telomeric zinc finger protein ZBTB48bioRxiv - Molecular Biology 2024Quote: The HEK293 Flp-In T-REx cell line was obtained from Life Technologies (Invitrogen catalogue number R780-07). HEK293 and HCT-116 cells (isolated from the colon of an adult male ...
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bioRxiv - Developmental Biology 2020Quote: Mouse menisci were dissected from tibiae of 4-week-old mice and digested in 0.25% Trypsin-EDTA (Gibco) for 1 h followed by 300U/mL collagenase type I (Worthington Biochemical ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated for 4 hrs in Alexafluor donkey anti-Rabbit 488 and donkey anti-Mouse 594 (Thermofisher). Sections were mounted ...
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bioRxiv - Molecular Biology 2020Quote: ... fixed with 4% PFA and incubated with anti-rat AlexaFluor 594 and anti-mouse AlexaFluor 488 (Thermo Fisher) for 1.5 h ...
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bioRxiv - Cancer Biology 2024Quote: Mouse prostates were isolated and fixed in 10% neutral-buffered formalin (SF100-4, Thermo Fisher Scientific, Waltham, MA) overnight ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibody was donkey anti-mouse Alexa 488 (4 μg/mL; Molecular Probes, catalog# A-21202, RRID: AB_141607). Slices were mounted on slides with Vectashield mounting medium containing DAPI (Vector Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... AlexaFluor 488 anti-rabbit (1:1000, green) and 568 anti-mouse (1:1000, red) (both Invitrogen; Table 4) secondary antibodies were also diluted in 1% BSA and 10 % goat serum in TBS and incubated in the dark for 45 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Fluo-4 (Fluo 4 AM/Fluo 4 direct, Life Technologies) was used according to the manufacturer’s instructions and a previously published protocol (54) ...