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Citations for Thermo Fisher :
1501 - 1550 of 10000+ citations for Cyclic AMP Direct ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2021Quote: ... mRNA was extracted from the FANS-isolated VC nuclei and SC nuclei of control and TL-10/+ (about 106 nuclei per sample) with independent biological replicates using a Dynabeads™ mRNA DIRECT™ Micro Purification Kit (Life Technologies). Total RNA of late microspore (about 106 microspores per sample ...
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bioRxiv - Genetics 2022Quote: ... PCR was done to confirm the presence of the construct using F-130WH Phire Plant Direct PCR Kit (Thermo Fisher Scientific, Waltham, Massachusetts) and Sanger sequencing.
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bioRxiv - Genomics 2021Quote: ... poly(A)+ RNA was extracted by incubating 100 µg total RNA with 200 µl Dynabeads solution (Dynabeads mRNA Direct Purification Kit, Thermo Fisher Scientific, 61012) and purified following manufacturer’s protocols.
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bioRxiv - Immunology 2021Quote: ... Total mRNA was extracted from 190 pMHC-tetramerpos purified (Supplementary Figure 1A) virus-specific T-cell populations(37) using magnetic beads (Dynabead mRNA DIRECT kit; Invitrogen, Thermo Fisher Scientific). Ten μl (~1μg ...
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bioRxiv - Genetics 2022Quote: ... All amplicons were screened for sequence variations by direct sequencing using Big-Dye Terminator v3.1 sequencing kit (Applied Biosystems, Foster City, CA, USA) and ABI 3130 Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... 100 ng of RNA from each sample was then reverse transcribed into cDNA using SSIV Cell Direct cDNA Synthesis kit (lot# 00916637, Invitrogen, ThermoFisher Scientific, Vilnius, Lithuania). Subsequently ...
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bioRxiv - Molecular Biology 2023Quote: ... The RNA sequencing library was generated from 200 ng total RNA using Dynabeads® mRNA DIRECT™ Micro Purification Kit (Thermo Fisher Scientific) for mRNA purification followed by NEBNext® Ultra™ II Directional RNA Library Prep Kit (New England BioLabs ...
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bioRxiv - Cancer Biology 2023Quote: 15,000 CD133+ were FACS sorted into 40 μL of lysis binding buffer and mRNA was isolated using Dynabeads mRNA DIRECT Purification Kit (Thermo-Fisher scientific; cat# 61012) according to manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The purified PCR products were Sanger sequenced on a ThermoFisher SeqStudio Genetic Analyzer using the original reverse primer and BigDye® Direct Cycle Sequencing kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Genetics 2021Quote: ... Total protein concentrations were then assessed using Direct Detect (ThermoFisher Scientific) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... electron microscope equipped with a Falcon3 direct electron-counting camera (ThermoFisher). Cryo-EM data were acquired with EPU software (FEI ...
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bioRxiv - Neuroscience 2020Quote: Single RAGP neurons in lysis buffer (Cells Direct Lysis Buffer, Invitrogen) were processed for reverse transcription using SuperScript VILO Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... qPCR was performed by direct dye binding (SYBR Green, Applied Biosystems). Primers were designed using Primer3 and blasted on the NIH website (https://www.ncbi.nlm.nih.gov/tools/primer-blast/ ...
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bioRxiv - Biochemistry 2022Quote: ... electron microscope equipped with a Falcon3 direct electron-counting camera (ThermoFisher). Cryo-EM data were acquired with EPU software (FEI ...
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bioRxiv - Neuroscience 2021Quote: ... or direct method using Alexa Fluor Dyes (ThermoFisher Scientific, Waltham, MA). Nuclei were counterstained using 4′,6-diamidino-2-phenylindole (DAPI) ...
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bioRxiv - Neuroscience 2020Quote: Single RAGP neurons in lysis buffer (Cells Direct Lysis Buffer, Invitrogen) were directly processed for reverse transcriptase reaction using SuperScript VILO Master Mix (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... equipped with Falcon II direct electron detector (Thermo Fisher Scientific, USA) and Cs image corrector (CEOS ...
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bioRxiv - Microbiology 2022Quote: ... equipped with a Falcon 3 direct electron detector (Thermo Fisher Scientific) for cryo-ET data collection ...
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bioRxiv - Cell Biology 2022Quote: ... equipped with a Falcon 3EC direct electron detector (Thermo Fisher Scientific). The autogrids were rotated 90° between Aquilos-2 and the respective electron microscope to optimize the relationship between the tilt-axis and the lamella orientation ...
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bioRxiv - Neuroscience 2024Quote: Cells were harvested by direct lysis in TRIzol Reagent (Life Technologies). RNA isolation was performed via phase separation according to manufacturers’ protocol ...
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bioRxiv - Evolutionary Biology 2023Quote: ... transformed isolates were genotyped by Phire Plant Direct PCR (Thermo Scientific) using primers described in S7 Table ...
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bioRxiv - Biophysics 2023Quote: ... equipped with a direct electron detector (Falcon IV, Thermo Fisher Scientific). Samples of desired ice thickness were used for data collection in a 300 kV electron-microscope (Titan Krios G3i ...
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bioRxiv - Molecular Biology 2023Quote: ... 10µL 2x Phire Tissue Direct PCR Master Mix (ThermoFisher Scientific, USA) was used ...
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bioRxiv - Biophysics 2023Quote: ... equipped with a Falcon 4 direct electron detector (Thermo Fisher Scientific) and a Gatan BioContinuum energy filter ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Calcium mobilization was measured using Fluo-4 Direct calcium dye (Invitrogen). HEK293 cell lines stably transfected with 5-HT2A ...
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bioRxiv - Biochemistry 2024Quote: ... with a Falcon 3EC direct electron detector (Thermo Fisher Scientific, USA) and operated at 200 kV using the EPU software (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... equipped with a Falcon 4i direct electron detector (Thermo Fisher Scientific). Fifty representative micrographs were recorded using EPU v3.4.0.5704 (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... the Phire Plant Direct PCR Master Mix (Thermo Scientific™, F160L) was used according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... (shown in Figure 6E,F) were cloned to pDEST-pGADT7 (Rossignol, 2007) and pDEST-pGBKT7-Amp (Galletta, 2014) using Gateway cloning system (Life Technologies) and transformed into Y187 and Y2HGold strains ...
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bioRxiv - Neuroscience 2022Quote: ... Mobile phases A and B were with 0.1% formic acid in water and 0.1% formic acid (Fisher Scientific; Cat#: A11710X1-AMP) in ACN (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The PCR touchdown protocol was applied in a Mini Amp Plus thermal cycler (Applied Biosystems, Thermo Fisher Scientific, Waltham, MA, USA) as follows ...
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bioRxiv - Biochemistry 2023Quote: ... One third of the reverse transcription mix was subjected to PCR amplification with TaqMan Universal PCR Master Mix No Amp Erase (Applied Biosystems) and the respective TaqMan reagents for target miRNA ...
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bioRxiv - Molecular Biology 2024Quote: CryoEM data of AMP-PNP-bound form and apo form of Polθ-hel were collected in a Glacios (Thermo Fisher Scientific) equipped with Falcon-4 direct electron detector operated at 200 kV in electron counting mode ...
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bioRxiv - Synthetic Biology 2022Quote: ... Selective plates contained 15 g L-1 Noble agar (Affymetrix) to eliminate bacterial growth on carbon source contaminants available in the agar ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM DTT using Zeba spin desalting plates (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Cell lysates used in immunoblotting analyses were prepared by direct lysis of drug-treated and untreated cells in 1× LDS–PAGE loading buffer (Thermo Fisher) after removal of cell culture media ...
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bioRxiv - Neuroscience 2022Quote: ... Live cultures or thawed cells were either expanded as floor plate progenitors (following Brundin approach; Floor Plate Progenitor Kit expansion protocol (ThermoFisher; A3165801) or terminally differentiated to DA neurons with all prior factors except in neurobasal base medium after day 25.
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bioRxiv - Immunology 2023Quote: ... A 10 μl supernatant sample was mixed with 90 μl substrate solution containing 0.5 mg/ml of 4-methylumbelliferyl N,N’-diacetyl-β-D -chitobioside in an assay buffer provided as part of the kit and in a 96-well plate (black/clear bottom plate, Thermo Fisher). The reaction was incubated at 37 °C for 30 min and then stopped by adding 100 μl stop solution ...
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bioRxiv - Immunology 2021Quote: ... TNF-α and IL-6 were measured according to the manufacturer’s instructions in plasma and peritoneal lavage supernatant using Novex ELISA Kits (Thermofisher Scientific Ltd.).
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bioRxiv - Pathology 2019Quote: ... IL-6 and IL-10 levels were assessed using ELISA kits (Cat# 88-7324-22, BMS603-2, 88-7105-22 Thermofisher, USA). The intensity of the colour was measured using a microplate reader (Thermal ...
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bioRxiv - Neuroscience 2019Quote: Aß1-40 and Aß1-42 concentrations from cleared supernatants of differentiated iPSCs were quantified by using the Amyloid beta 40/ 42 Human ELISA Kits (#KHB3441 and KHB3481; Thermo Fisher) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... IL-23 and tumor necrosis factor alpha (TNF-α) were quantified in lung tissue lysates and BAL using commercial ELISA kits (Invitrogen, San Diego ...
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bioRxiv - Immunology 2021Quote: ... the concentrations of human IL-2 and IFN-γ were measured using a IL-2 Human Uncoated ELISA Kit (Thermo Fisher) and IFN-γ Human Uncoated ELISA Kit (Invitrogen) ...
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bioRxiv - Biochemistry 2020Quote: ... to improve sensitivity and loading in ELISA or BLItz binding assays (EZ-Link™ Sulfo-NHS-LC-Biotinylation Kit, ThermoFisher 21435).
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bioRxiv - Molecular Biology 2021Quote: ... The concentration of IL-2 in 2x diluted supernatants was measured using an IL-2 mouse ELISA kit (Thermo Fisher Scientific) as per the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... IL-6 and TNF-α in RAW264.7 cells culture supernatants were analyzed using ELISA kits (No. EMIL6RA, 88-7324-22, bms6002; Thermo Scientific) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... of hiPSC-aCMs and -vCMs were measured by a competitive enzyme-linked immunosorbent assay (ELISA) using a commercially available kit (Invitrogen, CA). The assay was conducted according to the manufacturer’s protocol and was measured using a spectrophotometric plate reader.
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bioRxiv - Cell Biology 2020Quote: ... The concentration of cAMP in the lysates was then measured by using a competitive ELISA kit for cAMP (Invitrogen, ref. EMSCAMPL) following the recommendations of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... The cells were incubated overnight and IFNγ levels in the supernatant were detected using the human IFN-γ ELISA Ready-SET-Go! kit (Invitrogen) accorded to manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: ... TNF-α was measured using a commercially available equine TNF-α ELISA reagent kit (ESS0017; Thermo Fisher Scientific, Frederick, MD, USA), with anti-equine TNF-α coating antibody and biotinylated anti-equine TNF-α detection antibody both diluted 1:100 ...